Genotyping and antimicrobial resistance of Streptococcus uberis isolated from bovine clinical mastitis
A genotypic characterization of Streptococcus uberis isolated from clinical mastitis (CM) in dairy cows, and the association of Strep. uberis genotypes and antimicrobial susceptibility (AMS) was performed. A total of 89 isolates identified as Strep. uberis from 86 dairy cows with CM in 17 dairy herd...
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| Veröffentlicht in: | PloS one 2019-10, Vol.14 (10), p.e0223719-e0223719 |
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| description | A genotypic characterization of Streptococcus uberis isolated from clinical mastitis (CM) in dairy cows, and the association of Strep. uberis genotypes and antimicrobial susceptibility (AMS) was performed. A total of 89 isolates identified as Strep. uberis from 86 dairy cows with CM in 17 dairy herds of Southeastern Brazil were genotyped using random amplified polymorphic DNA (RAPD) analysis. After genotyping, two clusters (I and II) were created according to RAPD types. A commercial broth microdilution test was used to determine the susceptibility of Strep. uberis isolates to 8 antimicrobials (ampicillin, ceftiofur, cephalothin, erythromycin, penicillin, penicillin+novobiocin, pirlimycin and tetracycline). For each antimicrobial, we determined the minimal inhibitory concentrations that inhibit 50% (MIC50) and 90% (MIC90) of Strep. uberis strains. Differences in AMS among genotypic clusters were evaluated using mixed regression models. Overall, a great polymorphism (56 RAPD-types) was found among Strep. uberis isolates, although a higher genetic similarity (based on the PCR bands features) was observed within herds after genotypic clustering. No differences in AMS were observed among clusters. Strep. uberis isolated from bovine CM were resistant to most antimicrobials, with the exception of cephalothin and penicillin+novobiocin. |
| doi_str_mv | 10.1371/journal.pone.0223719 |
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A total of 89 isolates identified as Strep. uberis from 86 dairy cows with CM in 17 dairy herds of Southeastern Brazil were genotyped using random amplified polymorphic DNA (RAPD) analysis. After genotyping, two clusters (I and II) were created according to RAPD types. A commercial broth microdilution test was used to determine the susceptibility of Strep. uberis isolates to 8 antimicrobials (ampicillin, ceftiofur, cephalothin, erythromycin, penicillin, penicillin+novobiocin, pirlimycin and tetracycline). For each antimicrobial, we determined the minimal inhibitory concentrations that inhibit 50% (MIC50) and 90% (MIC90) of Strep. uberis strains. Differences in AMS among genotypic clusters were evaluated using mixed regression models. Overall, a great polymorphism (56 RAPD-types) was found among Strep. uberis isolates, although a higher genetic similarity (based on the PCR bands features) was observed within herds after genotypic clustering. No differences in AMS were observed among clusters. Strep. uberis isolated from bovine CM were resistant to most antimicrobials, with the exception of cephalothin and penicillin+novobiocin.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0223719</identifier><identifier>PMID: 31639136</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Ampicillin ; Animals ; Anti-Bacterial Agents - pharmacology ; Antibacterial agents ; Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial agents ; Antimicrobial resistance ; Bacteria ; Behavior ; Biology and Life Sciences ; Care and treatment ; Cattle ; Causes of ; Cephalothin ; Clustering ; Councils ; Dairy cattle ; Deoxyribonucleic acid ; Diseases ; DNA ; Drug resistance ; Drug Resistance, Bacterial ; EDTA ; Epidemiology ; Erythromycin ; Female ; Gene polymorphism ; Genes, Bacterial ; Genetic aspects ; Genotype ; Genotypes ; Genotyping ; Identification ; Identification and classification ; Laboratories ; Mastitis ; Mastitis, Bovine - microbiology ; Medicine and Health Sciences ; Methicillin ; Methods ; Microbial drug resistance ; Microbial Sensitivity Tests ; Milk ; Novobiocin ; Nutrition ; Oxacillin ; Penicillin ; Penicillins ; Phylogeny ; Polymerase chain reaction ; Polymorphism ; Prevention ; Random amplified polymorphic DNA ; Regression analysis ; Regression models ; Research and analysis methods ; Streptococcal Infections - veterinary ; Streptococcus ; Streptococcus - classification ; Streptococcus - drug effects ; Streptococcus - genetics ; Streptococcus uberis ; Tetracyclines ; Veterinary colleges ; Veterinary medicine ; Zoonoses</subject><ispartof>PloS one, 2019-10, Vol.14 (10), p.e0223719-e0223719</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Tomazi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Tomazi et al 2019 Tomazi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-d738291c28554dd6f5008c98fff7015fc4c3a5fa265cca8473070914469cbdb23</citedby><cites>FETCH-LOGICAL-c692t-d738291c28554dd6f5008c98fff7015fc4c3a5fa265cca8473070914469cbdb23</cites><orcidid>0000-0002-4273-3494</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805098/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805098/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31639136$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Yildirim, Arda</contributor><creatorcontrib>Tomazi, Tiago</creatorcontrib><creatorcontrib>Freu, Gustavo</creatorcontrib><creatorcontrib>Alves, Bruna Gomes</creatorcontrib><creatorcontrib>de Souza Filho, Antonio Francisco</creatorcontrib><creatorcontrib>Heinemann, Marcos Bryan</creatorcontrib><creatorcontrib>Veiga Dos Santos, Marcos</creatorcontrib><title>Genotyping and antimicrobial resistance of Streptococcus uberis isolated from bovine clinical mastitis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A genotypic characterization of Streptococcus uberis isolated from clinical mastitis (CM) in dairy cows, and the association of Strep. uberis genotypes and antimicrobial susceptibility (AMS) was performed. A total of 89 isolates identified as Strep. uberis from 86 dairy cows with CM in 17 dairy herds of Southeastern Brazil were genotyped using random amplified polymorphic DNA (RAPD) analysis. After genotyping, two clusters (I and II) were created according to RAPD types. A commercial broth microdilution test was used to determine the susceptibility of Strep. uberis isolates to 8 antimicrobials (ampicillin, ceftiofur, cephalothin, erythromycin, penicillin, penicillin+novobiocin, pirlimycin and tetracycline). For each antimicrobial, we determined the minimal inhibitory concentrations that inhibit 50% (MIC50) and 90% (MIC90) of Strep. uberis strains. Differences in AMS among genotypic clusters were evaluated using mixed regression models. Overall, a great polymorphism (56 RAPD-types) was found among Strep. uberis isolates, although a higher genetic similarity (based on the PCR bands features) was observed within herds after genotypic clustering. No differences in AMS were observed among clusters. 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microbiology</subject><subject>Medicine and Health Sciences</subject><subject>Methicillin</subject><subject>Methods</subject><subject>Microbial drug resistance</subject><subject>Microbial Sensitivity Tests</subject><subject>Milk</subject><subject>Novobiocin</subject><subject>Nutrition</subject><subject>Oxacillin</subject><subject>Penicillin</subject><subject>Penicillins</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Prevention</subject><subject>Random amplified polymorphic DNA</subject><subject>Regression analysis</subject><subject>Regression models</subject><subject>Research and analysis methods</subject><subject>Streptococcal Infections - veterinary</subject><subject>Streptococcus</subject><subject>Streptococcus - classification</subject><subject>Streptococcus - drug effects</subject><subject>Streptococcus - genetics</subject><subject>Streptococcus uberis</subject><subject>Tetracyclines</subject><subject>Veterinary colleges</subject><subject>Veterinary medicine</subject><subject>Zoonoses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99rFDEQxxdRbK3-B6ILgujDnfmxm928CKVoPSgUrPoaZrPJXUo2OZNssf-9ud623EofJISEyWe-k0xmiuI1RktMG_zp2o_BgV1uvVNLREi28SfFMeaULBhB9OnB_qh4EeM1QjVtGXteHFHMKMeUHRf6XDmfbrfGrUtwfZ7JDEYG3xmwZVDRxAROqtLr8ioFtU1eeinHWI6dCiaWJnoLSfWlDn4oO39jnCqlNc7ILDBATCaZ-LJ4psFG9WpaT4qfX7_8OPu2uLg8X52dXiwk4yQt-oa2hGNJ2rqu-p7pGqFW8lZr3SBca1lJCrUGwmopoa0aihrEcVUxLru-I_SkeLvX3VofxZSiKEjmGoIZrjKx2hO9h2uxDWaAcCs8GHFn8GEtICQjrRKYSs3bhnSq1xUH3Oq6QqoC1FGkKEDW-jxFG7tB9VK5FMDOROcnzmzE2t8I1qIa8TYLfJgEgv89qpjEYKJU1oJTfry7d4s5YWyHvvsHffx1E7WG_ADjtM9x5U5UnDJEq4YgstNaPkLl0av897metMn2mcPHmUNmkvqT1jDGKFZX3_-fvfw1Z98fsBsFNm1yPY3JeBfnYLUHc2XGGJR-SDJGYtcO99kQu3YQUztktzeHH_TgdF__9C8HKQYy</recordid><startdate>20191022</startdate><enddate>20191022</enddate><creator>Tomazi, Tiago</creator><creator>Freu, Gustavo</creator><creator>Alves, Bruna Gomes</creator><creator>de Souza Filho, Antonio Francisco</creator><creator>Heinemann, Marcos Bryan</creator><creator>Veiga Dos Santos, Marcos</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4273-3494</orcidid></search><sort><creationdate>20191022</creationdate><title>Genotyping and antimicrobial resistance of Streptococcus uberis isolated from bovine clinical mastitis</title><author>Tomazi, Tiago ; Freu, Gustavo ; Alves, Bruna Gomes ; de Souza Filho, Antonio Francisco ; Heinemann, Marcos Bryan ; Veiga Dos Santos, Marcos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-d738291c28554dd6f5008c98fff7015fc4c3a5fa265cca8473070914469cbdb23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Ampicillin</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - 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A total of 89 isolates identified as Strep. uberis from 86 dairy cows with CM in 17 dairy herds of Southeastern Brazil were genotyped using random amplified polymorphic DNA (RAPD) analysis. After genotyping, two clusters (I and II) were created according to RAPD types. A commercial broth microdilution test was used to determine the susceptibility of Strep. uberis isolates to 8 antimicrobials (ampicillin, ceftiofur, cephalothin, erythromycin, penicillin, penicillin+novobiocin, pirlimycin and tetracycline). For each antimicrobial, we determined the minimal inhibitory concentrations that inhibit 50% (MIC50) and 90% (MIC90) of Strep. uberis strains. Differences in AMS among genotypic clusters were evaluated using mixed regression models. Overall, a great polymorphism (56 RAPD-types) was found among Strep. uberis isolates, although a higher genetic similarity (based on the PCR bands features) was observed within herds after genotypic clustering. No differences in AMS were observed among clusters. Strep. uberis isolated from bovine CM were resistant to most antimicrobials, with the exception of cephalothin and penicillin+novobiocin.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31639136</pmid><doi>10.1371/journal.pone.0223719</doi><tpages>e0223719</tpages><orcidid>https://orcid.org/0000-0002-4273-3494</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Ampicillin Animals Anti-Bacterial Agents - pharmacology Antibacterial agents Antibiotics Antiinfectives and antibacterials Antimicrobial agents Antimicrobial resistance Bacteria Behavior Biology and Life Sciences Care and treatment Cattle Causes of Cephalothin Clustering Councils Dairy cattle Deoxyribonucleic acid Diseases DNA Drug resistance Drug Resistance, Bacterial EDTA Epidemiology Erythromycin Female Gene polymorphism Genes, Bacterial Genetic aspects Genotype Genotypes Genotyping Identification Identification and classification Laboratories Mastitis Mastitis, Bovine - microbiology Medicine and Health Sciences Methicillin Methods Microbial drug resistance Microbial Sensitivity Tests Milk Novobiocin Nutrition Oxacillin Penicillin Penicillins Phylogeny Polymerase chain reaction Polymorphism Prevention Random amplified polymorphic DNA Regression analysis Regression models Research and analysis methods Streptococcal Infections - veterinary Streptococcus Streptococcus - classification Streptococcus - drug effects Streptococcus - genetics Streptococcus uberis Tetracyclines Veterinary colleges Veterinary medicine Zoonoses |
| title | Genotyping and antimicrobial resistance of Streptococcus uberis isolated from bovine clinical mastitis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T20%3A48%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genotyping%20and%20antimicrobial%20resistance%20of%20Streptococcus%20uberis%20isolated%20from%20bovine%20clinical%20mastitis&rft.jtitle=PloS%20one&rft.au=Tomazi,%20Tiago&rft.date=2019-10-22&rft.volume=14&rft.issue=10&rft.spage=e0223719&rft.epage=e0223719&rft.pages=e0223719-e0223719&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0223719&rft_dat=%3Cgale_plos_%3EA603472028%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2307721614&rft_id=info:pmid/31639136&rft_galeid=A603472028&rft_doaj_id=oai_doaj_org_article_13cf9872bedf49a18f540e4a0b30e3aa&rfr_iscdi=true |