Identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2D-DIGE approach
A comparative analysis of blood samples (depleted of albumin and IgG) obtained from lung cancer patients before chemotherapy versus after a second cycle of chemotherapy was performed using two-dimensional difference gel electrophoresis (2D-DIGE). The control group consisted of eight patients with no...
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| creator | Ciereszko, Andrzej Dietrich, Mariola A Słowińska, Mariola Nynca, Joanna Ciborowski, Michał Kisluk, Joanna Michalska-Falkowska, Anna Reszec, Joanna Sierko, Ewa Nikliński, Jacek |
| description | A comparative analysis of blood samples (depleted of albumin and IgG) obtained from lung cancer patients before chemotherapy versus after a second cycle of chemotherapy was performed using two-dimensional difference gel electrophoresis (2D-DIGE). The control group consisted of eight patients with non-cancerous lung diseases, and the experimental group consisted of four adenocarcinoma (ADC) and four squamous cell carcinoma (SCC) patients. Analyses of gels revealed significant changes in proteins and/or their proteoforms between control patients and lung cancer patients, both before and after a second cycle of chemotherapy. Most of these proteins were related to inflammation, including acute phase proteins (APPs) such as forms of haptoglobin and transferrin, complement component C3, and clusterin. The variable expression of APPs can potentially be used for profiling lung cancer. The greatest changes observed after chemotherapy were in transferrin and serotransferrin, which likely reflect disturbances in iron turnover after chemotherapy-induced anaemia. Significant changes in plasma proteins between ADC and SCC patients were also revealed, suggesting use of plasma vitronectin as a potential marker of SCC. |
| doi_str_mv | 10.1371/journal.pone.0223840 |
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The control group consisted of eight patients with non-cancerous lung diseases, and the experimental group consisted of four adenocarcinoma (ADC) and four squamous cell carcinoma (SCC) patients. Analyses of gels revealed significant changes in proteins and/or their proteoforms between control patients and lung cancer patients, both before and after a second cycle of chemotherapy. Most of these proteins were related to inflammation, including acute phase proteins (APPs) such as forms of haptoglobin and transferrin, complement component C3, and clusterin. The variable expression of APPs can potentially be used for profiling lung cancer. The greatest changes observed after chemotherapy were in transferrin and serotransferrin, which likely reflect disturbances in iron turnover after chemotherapy-induced anaemia. Significant changes in plasma proteins between ADC and SCC patients were also revealed, suggesting use of plasma vitronectin as a potential marker of SCC.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0223840</identifier><identifier>PMID: 31622403</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acute phase proteins ; Acute phase substances ; Adenocarcinoma ; Adenocarcinoma - blood ; Adenocarcinoma - drug therapy ; Adenocarcinoma - pathology ; Aged ; Albumins ; Anemia ; Antigens ; Antineoplastic Agents - therapeutic use ; Biology and Life Sciences ; Biomarkers ; Biomarkers, Tumor - blood ; Biomarkers, Tumor - metabolism ; Blood ; Blood plasma ; Blood Proteins - analysis ; Blood Proteins - metabolism ; Breast cancer ; Cancer therapies ; Carcinoma, Squamous Cell - blood ; Carcinoma, Squamous Cell - drug therapy ; Carcinoma, Squamous Cell - pathology ; Chemotherapy ; Clusterin ; Comparative analysis ; Complement component C3 ; Electrophoresis ; Female ; Food ; Gel electrophoresis ; Gels ; Growth factors ; Haptoglobin ; Humans ; Immunoglobulin G ; Iron ; Labeling ; Lung cancer ; Lung diseases ; Lung Neoplasms - blood ; Lung Neoplasms - drug therapy ; Lung Neoplasms - pathology ; Male ; Mass Spectrometry ; Medical prognosis ; Medicine and Health Sciences ; Middle Aged ; Molecular biology ; Phosphorylation ; Plasma proteins ; Proteins ; Proteomics ; Research and Analysis Methods ; Squamous cell carcinoma ; Transferrin ; Transferrin - metabolism ; Transferrins ; Two dimensional analysis ; Two-Dimensional Difference Gel Electrophoresis - methods ; Vitronectin ; VOCs ; Volatile organic compounds</subject><ispartof>PloS one, 2019-10, Vol.14 (10), p.e0223840-e0223840</ispartof><rights>2019 Ciereszko et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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The control group consisted of eight patients with non-cancerous lung diseases, and the experimental group consisted of four adenocarcinoma (ADC) and four squamous cell carcinoma (SCC) patients. Analyses of gels revealed significant changes in proteins and/or their proteoforms between control patients and lung cancer patients, both before and after a second cycle of chemotherapy. Most of these proteins were related to inflammation, including acute phase proteins (APPs) such as forms of haptoglobin and transferrin, complement component C3, and clusterin. The variable expression of APPs can potentially be used for profiling lung cancer. The greatest changes observed after chemotherapy were in transferrin and serotransferrin, which likely reflect disturbances in iron turnover after chemotherapy-induced anaemia. 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The control group consisted of eight patients with non-cancerous lung diseases, and the experimental group consisted of four adenocarcinoma (ADC) and four squamous cell carcinoma (SCC) patients. Analyses of gels revealed significant changes in proteins and/or their proteoforms between control patients and lung cancer patients, both before and after a second cycle of chemotherapy. Most of these proteins were related to inflammation, including acute phase proteins (APPs) such as forms of haptoglobin and transferrin, complement component C3, and clusterin. The variable expression of APPs can potentially be used for profiling lung cancer. The greatest changes observed after chemotherapy were in transferrin and serotransferrin, which likely reflect disturbances in iron turnover after chemotherapy-induced anaemia. Significant changes in plasma proteins between ADC and SCC patients were also revealed, suggesting use of plasma vitronectin as a potential marker of SCC.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31622403</pmid><doi>10.1371/journal.pone.0223840</doi><orcidid>https://orcid.org/0000-0002-2525-0941</orcidid><orcidid>https://orcid.org/0000-0003-3729-0518</orcidid><orcidid>https://orcid.org/0000-0002-1103-624X</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_2306488110 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acute phase proteins Acute phase substances Adenocarcinoma Adenocarcinoma - blood Adenocarcinoma - drug therapy Adenocarcinoma - pathology Aged Albumins Anemia Antigens Antineoplastic Agents - therapeutic use Biology and Life Sciences Biomarkers Biomarkers, Tumor - blood Biomarkers, Tumor - metabolism Blood Blood plasma Blood Proteins - analysis Blood Proteins - metabolism Breast cancer Cancer therapies Carcinoma, Squamous Cell - blood Carcinoma, Squamous Cell - drug therapy Carcinoma, Squamous Cell - pathology Chemotherapy Clusterin Comparative analysis Complement component C3 Electrophoresis Female Food Gel electrophoresis Gels Growth factors Haptoglobin Humans Immunoglobulin G Iron Labeling Lung cancer Lung diseases Lung Neoplasms - blood Lung Neoplasms - drug therapy Lung Neoplasms - pathology Male Mass Spectrometry Medical prognosis Medicine and Health Sciences Middle Aged Molecular biology Phosphorylation Plasma proteins Proteins Proteomics Research and Analysis Methods Squamous cell carcinoma Transferrin Transferrin - metabolism Transferrins Two dimensional analysis Two-Dimensional Difference Gel Electrophoresis - methods Vitronectin VOCs Volatile organic compounds |
| title | Identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2D-DIGE approach |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T15%3A26%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20protein%20changes%20in%20the%20blood%20plasma%20of%20lung%20cancer%20patients%20subjected%20to%20chemotherapy%20using%20a%202D-DIGE%20approach&rft.jtitle=PloS%20one&rft.au=Ciereszko,%20Andrzej&rft.date=2019-10-17&rft.volume=14&rft.issue=10&rft.spage=e0223840&rft.epage=e0223840&rft.pages=e0223840-e0223840&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0223840&rft_dat=%3Cproquest_plos_%3E2307130022%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2306488110&rft_id=info:pmid/31622403&rft_doaj_id=oai_doaj_org_article_ef3f0af264d44202a0e700073dc7b573&rfr_iscdi=true |