Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop

The present study mainly consists of a re-evaluation of the rate at which C12E8, a typical non-ionic detergent used for membrane studies, is able to dissociate from biological membranes, with sarcoplasmic reticulum membrane vesicles being used as an example. Utilizing a brominated derivative of C12E...

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Veröffentlicht in:	PloS one 2019-10, Vol.14 (10), p.e0222932-e0222932
Hauptverfasser:	Champeil, Philippe, de Foresta, Béatrice, Picard, Martin, Gauron, Carole, Georgin, Dominique, le Maire, Marc, Møller, Jesper V, Lenoir, Guillaume, Montigny, Cédric
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Biochemistry Biological membranes Biology Biology and Life Sciences Bromination Detergents Detergents - chemistry Detergents - pharmacology Dissociation Endoplasmic reticulum Engineering and Technology Experiments Filtration Filtration - methods Fluorescence Glass fibers Intracellular Membranes - metabolism Kinetics Life Sciences Lipids Membrane vesicles Membranes NMR Nuclear magnetic resonance Physical Sciences Proteins Reaction kinetics Research and Analysis Methods Sarcoplasmic reticulum Sarcoplasmic Reticulum - metabolism Spectrometry, Fluorescence Transport Vesicles - metabolism
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