The synergistic effects of TaAGP.L-B1 and TaSSIVb-D mutations in wheat lead to alterations of gene expression patterns and starch content in grain development
Starch is synthesized from a series of reactions catalyzed by enzymes. ADP-glucose pyrophosphorylase (AGPase) initiates the synthesis pathway and synthesizes ADP-glucose, the substrate of starch synthase (SS), of which SSIV is an isoform. Mutations of the AGPase subunit and SSIV-coding genes affect...
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| creator | Zhang, Shunlin Guo, Huijun Irshad, Ahsan Xie, Yongdun Zhao, Linshu Xiong, Hongchun Gu, Jiayu Zhao, Shirong Ding, Yuping Liu, Luxiang |
| description | Starch is synthesized from a series of reactions catalyzed by enzymes. ADP-glucose pyrophosphorylase (AGPase) initiates the synthesis pathway and synthesizes ADP-glucose, the substrate of starch synthase (SS), of which SSIV is an isoform. Mutations of the AGPase subunit and SSIV-coding genes affect starch content and cause variation in the number of granules. Here, we pyramided the functional mutation alleles of the AGPase subunit gene TaAGP.L-B1 and the SSIV-coding gene TaSSIVb-D to elucidate their synergistic effects on other key starch biosynthesis genes and their impact on starch content. Both the TaAGP.L-B1 and TaSSIVb-D genes were expressed in wheat grain development, and the expression level of TaAGP.L-B1 was higher than that of TaSSIVb-D. The TaAGP.L-B1 gene was downregulated in the agp.L-B1 single and agp.L-B1/ssIV-D double mutants at 12 to 18 days after flowering (DAF). TaSSIVb-D expression was significantly reduced at 6 DAF in both ssIV-D single and double mutants. In the agp.L-B1/ssIV-D double mutant, TaGBSSII was upregulated, while TaAGPSS, TaSSI, and TaSBEII were downregulated. Under the interaction of these genes, the total starch and amylopectin contents were significantly decreased in agp.L-B1 and agp.L-B1/ssIV-D mutants. The results suggested that the mutations of TaAGP.L-B1 and TaSSIVb-D genes resulted in variation in the expression patterns of the other four starch synthetic genes and led to a reduction in starch and amylopectin contents. These mutants could be used further as germplasm for resistant starch analysis. |
| doi_str_mv | 10.1371/journal.pone.0223783 |
| format | Article |
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ADP-glucose pyrophosphorylase (AGPase) initiates the synthesis pathway and synthesizes ADP-glucose, the substrate of starch synthase (SS), of which SSIV is an isoform. Mutations of the AGPase subunit and SSIV-coding genes affect starch content and cause variation in the number of granules. Here, we pyramided the functional mutation alleles of the AGPase subunit gene TaAGP.L-B1 and the SSIV-coding gene TaSSIVb-D to elucidate their synergistic effects on other key starch biosynthesis genes and their impact on starch content. Both the TaAGP.L-B1 and TaSSIVb-D genes were expressed in wheat grain development, and the expression level of TaAGP.L-B1 was higher than that of TaSSIVb-D. The TaAGP.L-B1 gene was downregulated in the agp.L-B1 single and agp.L-B1/ssIV-D double mutants at 12 to 18 days after flowering (DAF). TaSSIVb-D expression was significantly reduced at 6 DAF in both ssIV-D single and double mutants. In the agp.L-B1/ssIV-D double mutant, TaGBSSII was upregulated, while TaAGPSS, TaSSI, and TaSBEII were downregulated. Under the interaction of these genes, the total starch and amylopectin contents were significantly decreased in agp.L-B1 and agp.L-B1/ssIV-D mutants. The results suggested that the mutations of TaAGP.L-B1 and TaSSIVb-D genes resulted in variation in the expression patterns of the other four starch synthetic genes and led to a reduction in starch and amylopectin contents. These mutants could be used further as germplasm for resistant starch analysis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0223783</identifier><identifier>PMID: 31603940</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenosine diphosphate ; Alleles ; Amylopectin ; Amylopectin - metabolism ; B1 gene ; Binding sites ; Biology and Life Sciences ; Biosynthesis ; Biosynthetic Pathways ; Chemical reactions ; Crop science ; Edible Grain - genetics ; Edible Grain - growth & development ; Edible Grain - metabolism ; Engineering ; Engineering and Technology ; Enzymes ; Flowering ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Gene mutation ; Genes ; Genetic aspects ; Genomes ; Genomics ; Germplasm ; Glucose ; Glucose-1-Phosphate Adenylyltransferase - genetics ; Glucose-1-Phosphate Adenylyltransferase - metabolism ; Grain ; Laboratories ; Metabolism ; Mutagenesis ; Mutants ; Mutation ; Physical Sciences ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Rice ; Starch ; Starch - biosynthesis ; Starch synthase ; Starch Synthase - genetics ; Starch Synthase - metabolism ; Substrates ; Synergistic effect ; Triticum - genetics ; Triticum - growth & development ; Triticum - metabolism ; Wheat</subject><ispartof>PloS one, 2019-10, Vol.14 (10), p.e0223783</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Zhang et al 2019 Zhang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-feb04f71b6c3844b0406e0494bdd3c7c97a0a2724f2fc999a85c41c15e0620853</citedby><cites>FETCH-LOGICAL-c692t-feb04f71b6c3844b0406e0494bdd3c7c97a0a2724f2fc999a85c41c15e0620853</cites><orcidid>0000-0001-6912-1482</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788705/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788705/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31603940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Shunlin</creatorcontrib><creatorcontrib>Guo, Huijun</creatorcontrib><creatorcontrib>Irshad, Ahsan</creatorcontrib><creatorcontrib>Xie, Yongdun</creatorcontrib><creatorcontrib>Zhao, Linshu</creatorcontrib><creatorcontrib>Xiong, Hongchun</creatorcontrib><creatorcontrib>Gu, Jiayu</creatorcontrib><creatorcontrib>Zhao, Shirong</creatorcontrib><creatorcontrib>Ding, Yuping</creatorcontrib><creatorcontrib>Liu, Luxiang</creatorcontrib><title>The synergistic effects of TaAGP.L-B1 and TaSSIVb-D mutations in wheat lead to alterations of gene expression patterns and starch content in grain development</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Starch is synthesized from a series of reactions catalyzed by enzymes. ADP-glucose pyrophosphorylase (AGPase) initiates the synthesis pathway and synthesizes ADP-glucose, the substrate of starch synthase (SS), of which SSIV is an isoform. Mutations of the AGPase subunit and SSIV-coding genes affect starch content and cause variation in the number of granules. Here, we pyramided the functional mutation alleles of the AGPase subunit gene TaAGP.L-B1 and the SSIV-coding gene TaSSIVb-D to elucidate their synergistic effects on other key starch biosynthesis genes and their impact on starch content. Both the TaAGP.L-B1 and TaSSIVb-D genes were expressed in wheat grain development, and the expression level of TaAGP.L-B1 was higher than that of TaSSIVb-D. The TaAGP.L-B1 gene was downregulated in the agp.L-B1 single and agp.L-B1/ssIV-D double mutants at 12 to 18 days after flowering (DAF). TaSSIVb-D expression was significantly reduced at 6 DAF in both ssIV-D single and double mutants. In the agp.L-B1/ssIV-D double mutant, TaGBSSII was upregulated, while TaAGPSS, TaSSI, and TaSBEII were downregulated. Under the interaction of these genes, the total starch and amylopectin contents were significantly decreased in agp.L-B1 and agp.L-B1/ssIV-D mutants. The results suggested that the mutations of TaAGP.L-B1 and TaSSIVb-D genes resulted in variation in the expression patterns of the other four starch synthetic genes and led to a reduction in starch and amylopectin contents. These mutants could be used further as germplasm for resistant starch analysis.</description><subject>Adenosine diphosphate</subject><subject>Alleles</subject><subject>Amylopectin</subject><subject>Amylopectin - metabolism</subject><subject>B1 gene</subject><subject>Binding sites</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biosynthetic Pathways</subject><subject>Chemical reactions</subject><subject>Crop science</subject><subject>Edible Grain - genetics</subject><subject>Edible Grain - growth & development</subject><subject>Edible Grain - metabolism</subject><subject>Engineering</subject><subject>Engineering and Technology</subject><subject>Enzymes</subject><subject>Flowering</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene mutation</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Germplasm</subject><subject>Glucose</subject><subject>Glucose-1-Phosphate Adenylyltransferase - genetics</subject><subject>Glucose-1-Phosphate Adenylyltransferase - metabolism</subject><subject>Grain</subject><subject>Laboratories</subject><subject>Metabolism</subject><subject>Mutagenesis</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Physical Sciences</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Rice</subject><subject>Starch</subject><subject>Starch - biosynthesis</subject><subject>Starch synthase</subject><subject>Starch Synthase - genetics</subject><subject>Starch Synthase - metabolism</subject><subject>Substrates</subject><subject>Synergistic effect</subject><subject>Triticum - genetics</subject><subject>Triticum - growth & development</subject><subject>Triticum - metabolism</subject><subject>Wheat</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QDguBFx3z160YYV10HBlaccW9Dmp50unSamqTr7p_xt5qZ6S5TUJBC25zznLenb3Ki6CXBM8Iy8v7aDLaT7aw3HcwwpSzL2aPolBSMxinF7PHR-0n0zLlrjBOWp-nT6ISRFLOC49Po93oDyN11YOvG-UYh0BqUd8hotJbzi2-zZfyRINlVYblaLa7K-BPaDl76xnQONR36tQHpUQuyQt4g2XqwYzJI1NABgtvegnMhhnrpQz7kdoLOS6s2SJnOQ-d3WrWV4V7BDbSm34bg8-iJlq2DF-PzLPrx5fP6_Gu8vLxYnM-XsUoL6mMNJeY6I2WqWM55WOAUMC94WVVMZarIJJY0o1xTrYqikHmiOFEkARzsyRN2Fr0-6PatcWK01gnKMCc445gFYnEgKiOvRW-brbR3wshG7APG1kLaYGALoiJFWQKhJE8lLzNSJFRpUvAk9EJzVgatD-PXhnILlQo_amU7EZ1mumYjanMj0izPM7xr980oYM3PAZz_R8sjVcvQVdNpE8TUtnFKzFNMOc3DAQnU7C9UuCrYNmFvQDchPil4NynY79-tr-XgnFisvv8_e3k1Zd8eseFUtX7jTDvsD9MU5AdQWeOcBf3gHMFiNxv3bojdbIhxNkLZq2PXH4ruh4H9ASRKChA</recordid><startdate>20191011</startdate><enddate>20191011</enddate><creator>Zhang, Shunlin</creator><creator>Guo, Huijun</creator><creator>Irshad, Ahsan</creator><creator>Xie, Yongdun</creator><creator>Zhao, Linshu</creator><creator>Xiong, Hongchun</creator><creator>Gu, Jiayu</creator><creator>Zhao, Shirong</creator><creator>Ding, Yuping</creator><creator>Liu, Luxiang</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-6912-1482</orcidid></search><sort><creationdate>20191011</creationdate><title>The synergistic effects of TaAGP.L-B1 and TaSSIVb-D mutations in wheat lead to alterations of gene expression patterns and starch content in grain development</title><author>Zhang, Shunlin ; Guo, Huijun ; Irshad, Ahsan ; Xie, Yongdun ; Zhao, Linshu ; Xiong, Hongchun ; Gu, Jiayu ; Zhao, Shirong ; Ding, Yuping ; Liu, Luxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-feb04f71b6c3844b0406e0494bdd3c7c97a0a2724f2fc999a85c41c15e0620853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adenosine diphosphate</topic><topic>Alleles</topic><topic>Amylopectin</topic><topic>Amylopectin - metabolism</topic><topic>B1 gene</topic><topic>Binding sites</topic><topic>Biology and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biosynthetic Pathways</topic><topic>Chemical reactions</topic><topic>Crop science</topic><topic>Edible Grain - genetics</topic><topic>Edible Grain - growth & development</topic><topic>Edible Grain - metabolism</topic><topic>Engineering</topic><topic>Engineering and Technology</topic><topic>Enzymes</topic><topic>Flowering</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene mutation</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Germplasm</topic><topic>Glucose</topic><topic>Glucose-1-Phosphate Adenylyltransferase - genetics</topic><topic>Glucose-1-Phosphate Adenylyltransferase - metabolism</topic><topic>Grain</topic><topic>Laboratories</topic><topic>Metabolism</topic><topic>Mutagenesis</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Physical Sciences</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Rice</topic><topic>Starch</topic><topic>Starch - biosynthesis</topic><topic>Starch synthase</topic><topic>Starch Synthase - genetics</topic><topic>Starch Synthase - metabolism</topic><topic>Substrates</topic><topic>Synergistic effect</topic><topic>Triticum - genetics</topic><topic>Triticum - growth & development</topic><topic>Triticum - metabolism</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Shunlin</creatorcontrib><creatorcontrib>Guo, Huijun</creatorcontrib><creatorcontrib>Irshad, Ahsan</creatorcontrib><creatorcontrib>Xie, Yongdun</creatorcontrib><creatorcontrib>Zhao, Linshu</creatorcontrib><creatorcontrib>Xiong, Hongchun</creatorcontrib><creatorcontrib>Gu, Jiayu</creatorcontrib><creatorcontrib>Zhao, Shirong</creatorcontrib><creatorcontrib>Ding, Yuping</creatorcontrib><creatorcontrib>Liu, Luxiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints In Context: Health and Medicine</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Shunlin</au><au>Guo, Huijun</au><au>Irshad, Ahsan</au><au>Xie, Yongdun</au><au>Zhao, Linshu</au><au>Xiong, Hongchun</au><au>Gu, Jiayu</au><au>Zhao, Shirong</au><au>Ding, Yuping</au><au>Liu, Luxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The synergistic effects of TaAGP.L-B1 and TaSSIVb-D mutations in wheat lead to alterations of gene expression patterns and starch content in grain development</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-10-11</date><risdate>2019</risdate><volume>14</volume><issue>10</issue><spage>e0223783</spage><pages>e0223783-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Starch is synthesized from a series of reactions catalyzed by enzymes. ADP-glucose pyrophosphorylase (AGPase) initiates the synthesis pathway and synthesizes ADP-glucose, the substrate of starch synthase (SS), of which SSIV is an isoform. Mutations of the AGPase subunit and SSIV-coding genes affect starch content and cause variation in the number of granules. Here, we pyramided the functional mutation alleles of the AGPase subunit gene TaAGP.L-B1 and the SSIV-coding gene TaSSIVb-D to elucidate their synergistic effects on other key starch biosynthesis genes and their impact on starch content. Both the TaAGP.L-B1 and TaSSIVb-D genes were expressed in wheat grain development, and the expression level of TaAGP.L-B1 was higher than that of TaSSIVb-D. The TaAGP.L-B1 gene was downregulated in the agp.L-B1 single and agp.L-B1/ssIV-D double mutants at 12 to 18 days after flowering (DAF). TaSSIVb-D expression was significantly reduced at 6 DAF in both ssIV-D single and double mutants. In the agp.L-B1/ssIV-D double mutant, TaGBSSII was upregulated, while TaAGPSS, TaSSI, and TaSBEII were downregulated. Under the interaction of these genes, the total starch and amylopectin contents were significantly decreased in agp.L-B1 and agp.L-B1/ssIV-D mutants. The results suggested that the mutations of TaAGP.L-B1 and TaSSIVb-D genes resulted in variation in the expression patterns of the other four starch synthetic genes and led to a reduction in starch and amylopectin contents. These mutants could be used further as germplasm for resistant starch analysis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31603940</pmid><doi>10.1371/journal.pone.0223783</doi><tpages>e0223783</tpages><orcidid>https://orcid.org/0000-0001-6912-1482</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-10, Vol.14 (10), p.e0223783 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2304107403 |
| source | MEDLINE; Public Library of Science; Full-Text Journals in Chemistry (Open access); PubMed Central; Directory of Open Access Journals; EZB Electronic Journals Library |
| subjects | Adenosine diphosphate Alleles Amylopectin Amylopectin - metabolism B1 gene Binding sites Biology and Life Sciences Biosynthesis Biosynthetic Pathways Chemical reactions Crop science Edible Grain - genetics Edible Grain - growth & development Edible Grain - metabolism Engineering Engineering and Technology Enzymes Flowering Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Gene mutation Genes Genetic aspects Genomes Genomics Germplasm Glucose Glucose-1-Phosphate Adenylyltransferase - genetics Glucose-1-Phosphate Adenylyltransferase - metabolism Grain Laboratories Metabolism Mutagenesis Mutants Mutation Physical Sciences Plant Proteins - genetics Plant Proteins - metabolism Rice Starch Starch - biosynthesis Starch synthase Starch Synthase - genetics Starch Synthase - metabolism Substrates Synergistic effect Triticum - genetics Triticum - growth & development Triticum - metabolism Wheat |
| title | The synergistic effects of TaAGP.L-B1 and TaSSIVb-D mutations in wheat lead to alterations of gene expression patterns and starch content in grain development |
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