Comparative genomic analyses reveal diverse virulence factors and antimicrobial resistance mechanisms in clinical Elizabethkingia meningoseptica strains
Three human clinical isolates of bacteria (designated strains Em1, Em2 and Em3) had high average nucleotide identity (ANI) to Elizabethkingia meningoseptica. Their genome sizes (3.89, 4.04 and 4.04 Mb) were comparable to those of other Elizabethkingia species and strains, and exhibited open pan-geno...
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| description | Three human clinical isolates of bacteria (designated strains Em1, Em2 and Em3) had high average nucleotide identity (ANI) to Elizabethkingia meningoseptica. Their genome sizes (3.89, 4.04 and 4.04 Mb) were comparable to those of other Elizabethkingia species and strains, and exhibited open pan-genome characteristics, with two strains being nearly identical and the third divergent. These strains were susceptible only to trimethoprim/sulfamethoxazole and ciprofloxacin amongst 16 antibiotics in minimum inhibitory tests. The resistome exhibited a high diversity of resistance genes, including 5 different lactamase- and 18 efflux protein- encoding genes. Forty-four genes encoding virulence factors were conserved among the strains. Sialic acid transporters and curli synthesis genes were well conserved in E. meningoseptica but absent in E. anophelis and E. miricola. E. meningoseptica carried several genes contributing to biofilm formation. 58 glycoside hydrolases (GH) and 25 putative polysaccharide utilization loci (PULs) were found. The strains carried numerous genes encoding two-component system proteins (56), transcription factor proteins (187~191), and DNA-binding proteins (6~7). Several prophages and CRISPR/Cas elements were uniquely present in the genomes. |
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Their genome sizes (3.89, 4.04 and 4.04 Mb) were comparable to those of other Elizabethkingia species and strains, and exhibited open pan-genome characteristics, with two strains being nearly identical and the third divergent. These strains were susceptible only to trimethoprim/sulfamethoxazole and ciprofloxacin amongst 16 antibiotics in minimum inhibitory tests. The resistome exhibited a high diversity of resistance genes, including 5 different lactamase- and 18 efflux protein- encoding genes. Forty-four genes encoding virulence factors were conserved among the strains. Sialic acid transporters and curli synthesis genes were well conserved in E. meningoseptica but absent in E. anophelis and E. miricola. E. meningoseptica carried several genes contributing to biofilm formation. 58 glycoside hydrolases (GH) and 25 putative polysaccharide utilization loci (PULs) were found. 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Their genome sizes (3.89, 4.04 and 4.04 Mb) were comparable to those of other Elizabethkingia species and strains, and exhibited open pan-genome characteristics, with two strains being nearly identical and the third divergent. These strains were susceptible only to trimethoprim/sulfamethoxazole and ciprofloxacin amongst 16 antibiotics in minimum inhibitory tests. The resistome exhibited a high diversity of resistance genes, including 5 different lactamase- and 18 efflux protein- encoding genes. Forty-four genes encoding virulence factors were conserved among the strains. Sialic acid transporters and curli synthesis genes were well conserved in E. meningoseptica but absent in E. anophelis and E. miricola. E. meningoseptica carried several genes contributing to biofilm formation. 58 glycoside hydrolases (GH) and 25 putative polysaccharide utilization loci (PULs) were found. 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genetics</subject><subject>Trimethoprim</subject><subject>Viral infections</subject><subject>Virulence</subject><subject>Virulence (Microbiology)</subject><subject>Virulence factors</subject><subject>Virulence Factors - genetics</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk81q3DAQx01padJt36C0hkJpDrvVhy2tL4UQ0iYQCPTrKmbl8a5SW9pI9tL0TXrrs_TJOpvdhGzIoRhhMfrNXzOjmSx7ydmES83fX4Qhemgny-BxwoQQqpg-yvZ5JcVYCSYf39nvZc9SumCslFOlnmZ7kivGhCz2s99HoVtChN6tMJ-jD52zOZDuVcKUR1whtHlNhzFhvnJxaNFbzBuwfYiJyJpW78grhpkjNmJyqYc11KFdgHepS7nzuW2dd5aI49b9ghn2ix_Ozx0Q5mkTEi57Ov_7J_URnE_PsycNtAlfbP-j7NvH469HJ-Oz80-nR4dnY6uF6se1AGbLqcVmCmBhBoWcYlkgR1ayugHBC1lAWfKyEZohVo0quGy4ZbxqtEI5yl5vdJdtSGZb1WSEZLLSVcUUEacbog5wYZbRdRCvTABnrg0hzg1Eir1FwzUK0AwUQFVUQleiBF0WtVJ1rZEL0vqwvW2YdVhb9JRtuyO6e-LdwszDyihNT0cPOMoONgKLe24nh2dmbWOi0qKUxYoT-257WQyXA6bedC5ZbFvwGIbrHEsmdcHWcb25hz5ciS01B0rW-SZQjHYtag4VqXCuK0nU5AGKvhqpT6hdG0f2HYeDHQdievzZz2FIyZx--fz_7Pn3XfbtHXZBrdwvUmiH3gWfdsFiA1IXpxSxua0sZ2Y9bTfVMOtpM9tpI7dXdx_z1ulmvOQ_pOMotg</recordid><startdate>20191010</startdate><enddate>20191010</enddate><creator>Chen, Shicheng</creator><creator>Soehnlen, Marty</creator><creator>Blom, Jochen</creator><creator>Terrapon, Nicolas</creator><creator>Henrissat, Bernard</creator><creator>Walker, Edward D</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3693-6017</orcidid><orcidid>https://orcid.org/0000-0001-6455-3622</orcidid></search><sort><creationdate>20191010</creationdate><title>Comparative genomic analyses reveal diverse virulence factors and antimicrobial resistance mechanisms in clinical Elizabethkingia meningoseptica strains</title><author>Chen, Shicheng ; Soehnlen, Marty ; Blom, Jochen ; Terrapon, Nicolas ; Henrissat, Bernard ; Walker, Edward D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c726t-d2a0c58cef8aacaba438e54e1e050dfa21434a5515f270ee9f6413f1c019f76e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anti-Bacterial Agents - 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Their genome sizes (3.89, 4.04 and 4.04 Mb) were comparable to those of other Elizabethkingia species and strains, and exhibited open pan-genome characteristics, with two strains being nearly identical and the third divergent. These strains were susceptible only to trimethoprim/sulfamethoxazole and ciprofloxacin amongst 16 antibiotics in minimum inhibitory tests. The resistome exhibited a high diversity of resistance genes, including 5 different lactamase- and 18 efflux protein- encoding genes. Forty-four genes encoding virulence factors were conserved among the strains. Sialic acid transporters and curli synthesis genes were well conserved in E. meningoseptica but absent in E. anophelis and E. miricola. E. meningoseptica carried several genes contributing to biofilm formation. 58 glycoside hydrolases (GH) and 25 putative polysaccharide utilization loci (PULs) were found. 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| language | eng |
| recordid | cdi_plos_journals_2303979906 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Anti-Bacterial Agents - therapeutic use Antibiotics Antimicrobial agents Antimicrobial resistance Bacteria Binding proteins Biofilms Biofilms - growth & development Biology and Life Sciences Ciprofloxacin Clinical isolates Clustered Regularly Interspaced Short Palindromic Repeats - genetics Comparative Genomic Hybridization CRISPR Deoxyribonucleic acid DNA DNA sequencing DNA-binding protein DNA-Binding Proteins - genetics Drug resistance Drug Resistance, Bacterial - genetics Efflux Epidemics Flavobacteriaceae - genetics Flavobacteriaceae - pathogenicity Flavobacteriaceae Infections - drug therapy Flavobacteriaceae Infections - epidemiology Flavobacteriaceae Infections - genetics Flavobacteriaceae Infections - microbiology Gene expression Genes Genetic aspects Genome, Bacterial - genetics Genomes Genomic analysis Genomics Genomics - methods Glycoside hydrolase Gram-negative bacteria Gram-positive bacteria Growth hormone Humans Hydrolases Infections Laboratories Life Sciences Medicine and Health Sciences Microbial drug resistance Nucleotides Organic acids Patients Phylogeny Physical Sciences Polysaccharides Prophages Protein binding Proteins Resistance factors Strains (organisms) Studies Sulfamethoxazole Tetracyclines Transcription (Genetics) Transcription Factors - genetics Trimethoprim Viral infections Virulence Virulence (Microbiology) Virulence factors Virulence Factors - genetics |
| title | Comparative genomic analyses reveal diverse virulence factors and antimicrobial resistance mechanisms in clinical Elizabethkingia meningoseptica strains |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T12%3A13%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20genomic%20analyses%20reveal%20diverse%20virulence%20factors%20and%20antimicrobial%20resistance%20mechanisms%20in%20clinical%20Elizabethkingia%20meningoseptica%C2%A0strains&rft.jtitle=PloS%20one&rft.au=Chen,%20Shicheng&rft.date=2019-10-10&rft.volume=14&rft.issue=10&rft.spage=e0222648&rft.epage=e0222648&rft.pages=e0222648-e0222648&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0222648&rft_dat=%3Cgale_plos_%3EA602311793%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2303979906&rft_id=info:pmid/31600234&rft_galeid=A602311793&rft_doaj_id=oai_doaj_org_article_17e2a70a6aa94927925a754d66dd7e12&rfr_iscdi=true |