A pull-down and slot blot-based screening system for inhibitor compounds of the podoplanin-CLEC-2 interaction

Podoplanin, a transmembrane glycoprotein, is overexpressed in certain types of tumors and induces platelet aggregation by binding to C-type lectin-like receptor 2 (CLEC-2) on the platelet membrane. Activated platelets release granule components, which in turn, trigger epithelial-mesenchymal transiti...

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Veröffentlicht in:	PloS one 2019-09, Vol.14 (9), p.e0222331-e0222331
Hauptverfasser:	Watanabe, Nobuo, Kidokoro, Masako, Suzuki, Yusuke, Tanaka, Makiko, Inoue, Shigeaki, Tsukamoto, Hideo, Hirayama, Noriaki, Hsieh, Pei-Wen, Tseng, Ching-Ping, Nakagawa, Yoshihide, Inokuchi, Sadaki
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Baits Beads Biology and life sciences Blood platelets Cancer Cancer cells Cancer metastasis Cancer prevention Centrifugal filtration Centrifugation Chemical compounds Critical care Deoxyribonucleic acid DNA Drug Evaluation, Preclinical - methods Electrophoresis Fc receptors Fusion protein Gel electrophoresis Glycoproteins Health screening HeLa Cells Humans Immunoglobulin Fc Fragments - metabolism Immunoglobulins Inhibitors Invasiveness Laboratories Lectins Lectins, C-Type - antagonists & inhibitors Lectins, C-Type - metabolism Medicine Medicine and Health Sciences Membrane Glycoproteins - antagonists & inhibitors Membrane Glycoproteins - metabolism Mesenchyme Metastases Metastasis Pharmacology Plasmids Platelet aggregation Platelets Prevention Protein A Protein Binding Protein interaction Protein-protein interactions Proteins Recombinant Proteins Research and Analysis Methods Screening Stem cells Thrombosis Tumor cells Tumors Workload
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