Chemotactic migration of newly excysted juvenile Clonorchis sinensis is suppressed by neuro-antagonists
The metacercariae of the Clonorchis sinensis liver fluke excyst in the duodenum of mammalian hosts, and the newly excysted juveniles (CsNEJs) migrate along the bile duct via bile chemotaxis. Cholic acid is a major component of bile that induces this migration. We investigated the neuronal control of...
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| creator | Li, Shunyu Song, Jin-Ho Kim, Tae Im Yoo, Won Gi Won, Moo-Ho Dai, Fuhong Hong, Sung-Jong |
| description | The metacercariae of the Clonorchis sinensis liver fluke excyst in the duodenum of mammalian hosts, and the newly excysted juveniles (CsNEJs) migrate along the bile duct via bile chemotaxis. Cholic acid is a major component of bile that induces this migration. We investigated the neuronal control of chemotactic behavior of CsNEJs toward cholic acid. The migration of CsNEJs was strongly inhibited at sub-micromolar concentration by dopamine D1 (LE-300 and SKF-83566), D2 (spiramide, nemonapride, and sulpiride), and D3 (GR-103691 and NGB-2904) receptor antagonists, as well as a dopamine reuptake inhibitor (BTCP). Neuropeptides, FMRFamide, peptide YY, and neuropeptide Y were also potent inhibitors of chemotaxis. Meanwhile, serotonergic, glutamatergic, and cholinergic inhibitors did not affect chemotaxis, with the exception of fluoxetine and CNQX. Confocal immunofluorescence analysis indicated that dopaminergic and cholinergic neurons were colocalized in the somatic muscle tissues of adult C. sinensis. Our findings suggest that dopaminergic neurons and neuropeptides play a major role in the chemotactic migration of CsNEJs to bile, and their inhibitors or modulators could be utilized to prevent their migration from the bile duct. |
| doi_str_mv | 10.1371/journal.pntd.0007573 |
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Cholic acid is a major component of bile that induces this migration. We investigated the neuronal control of chemotactic behavior of CsNEJs toward cholic acid. The migration of CsNEJs was strongly inhibited at sub-micromolar concentration by dopamine D1 (LE-300 and SKF-83566), D2 (spiramide, nemonapride, and sulpiride), and D3 (GR-103691 and NGB-2904) receptor antagonists, as well as a dopamine reuptake inhibitor (BTCP). Neuropeptides, FMRFamide, peptide YY, and neuropeptide Y were also potent inhibitors of chemotaxis. Meanwhile, serotonergic, glutamatergic, and cholinergic inhibitors did not affect chemotaxis, with the exception of fluoxetine and CNQX. Confocal immunofluorescence analysis indicated that dopaminergic and cholinergic neurons were colocalized in the somatic muscle tissues of adult C. sinensis. Our findings suggest that dopaminergic neurons and neuropeptides play a major role in the chemotactic migration of CsNEJs to bile, and their inhibitors or modulators could be utilized to prevent their migration from the bile duct.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0007573</identifier><identifier>PMID: 31408466</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Animals ; Antidepressants ; Benzamides - pharmacology ; Bile ; Bile ducts ; Biology ; Biology and Life Sciences ; Biphenyl Compounds - pharmacology ; Care and treatment ; Cell migration ; Chemotaxis ; Chemotaxis - drug effects ; Chemotaxis - physiology ; Cholic Acid ; Cholinergics ; Cloning ; Clonorchis sinensis ; Clonorchis sinensis - drug effects ; Clonorchis sinensis - physiology ; Dopamine ; Dopamine - pharmacology ; Dopamine D1 receptors ; Dopamine D2 receptors ; Dopamine D3 receptors ; Dopamine Uptake Inhibitors - pharmacology ; Duodenum ; Excitatory Amino Acid Agents - pharmacology ; Fasciola hepatica - drug effects ; Fluke infections ; Fluorenes - pharmacology ; Fluorescent antibody technique ; Fluoxetine ; FMRFamide ; FMRFamide - pharmacology ; Glutamatergic transmission ; Immunofluorescence ; Immunomodulation ; Inhibitors ; Juveniles ; Laboratory animals ; Liver ; Medicine ; Migratory species ; Minors ; Modulators ; Motility ; Muscles ; Nervous system ; Neuromodulation ; Neurons ; Neuropeptide Y ; Neuropeptide Y - pharmacology ; Neuropeptides ; Neurosciences ; Neurotransmitter Agents - pharmacology ; Parasites ; Parasitology ; Peptide YY - pharmacology ; Peptides ; Phenols (Class of compounds) ; Physical Sciences ; Piperazines - pharmacology ; Pirenzepine ; Proteins ; Receptors ; Risk factors ; Serotonin ; Serotonin Agents - pharmacology ; Spiro Compounds - pharmacology ; Sulpiride ; Sulpiride - pharmacology ; Tropical diseases ; University colleges</subject><ispartof>PLoS neglected tropical diseases, 2019-08, Vol.13 (8), p.e0007573-e0007573</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Li et al 2019 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c690t-657d604c0df9755cbd9c7efcfba281e3395794ef06a68587baf0f4e8437fa22a3</citedby><cites>FETCH-LOGICAL-c690t-657d604c0df9755cbd9c7efcfba281e3395794ef06a68587baf0f4e8437fa22a3</cites><orcidid>0000-0002-8677-4714 ; 0000-0002-3041-5560</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691982/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691982/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31408466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Chai, Jong-Yil</contributor><creatorcontrib>Li, Shunyu</creatorcontrib><creatorcontrib>Song, Jin-Ho</creatorcontrib><creatorcontrib>Kim, Tae Im</creatorcontrib><creatorcontrib>Yoo, Won Gi</creatorcontrib><creatorcontrib>Won, Moo-Ho</creatorcontrib><creatorcontrib>Dai, Fuhong</creatorcontrib><creatorcontrib>Hong, Sung-Jong</creatorcontrib><title>Chemotactic migration of newly excysted juvenile Clonorchis sinensis is suppressed by neuro-antagonists</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>The metacercariae of the Clonorchis sinensis liver fluke excyst in the duodenum of mammalian hosts, and the newly excysted juveniles (CsNEJs) migrate along the bile duct via bile chemotaxis. Cholic acid is a major component of bile that induces this migration. We investigated the neuronal control of chemotactic behavior of CsNEJs toward cholic acid. The migration of CsNEJs was strongly inhibited at sub-micromolar concentration by dopamine D1 (LE-300 and SKF-83566), D2 (spiramide, nemonapride, and sulpiride), and D3 (GR-103691 and NGB-2904) receptor antagonists, as well as a dopamine reuptake inhibitor (BTCP). Neuropeptides, FMRFamide, peptide YY, and neuropeptide Y were also potent inhibitors of chemotaxis. Meanwhile, serotonergic, glutamatergic, and cholinergic inhibitors did not affect chemotaxis, with the exception of fluoxetine and CNQX. Confocal immunofluorescence analysis indicated that dopaminergic and cholinergic neurons were colocalized in the somatic muscle tissues of adult C. sinensis. Our findings suggest that dopaminergic neurons and neuropeptides play a major role in the chemotactic migration of CsNEJs to bile, and their inhibitors or modulators could be utilized to prevent their migration from the bile duct.</description><subject>Acids</subject><subject>Animals</subject><subject>Antidepressants</subject><subject>Benzamides - pharmacology</subject><subject>Bile</subject><subject>Bile ducts</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Biphenyl Compounds - pharmacology</subject><subject>Care and treatment</subject><subject>Cell migration</subject><subject>Chemotaxis</subject><subject>Chemotaxis - drug effects</subject><subject>Chemotaxis - physiology</subject><subject>Cholic Acid</subject><subject>Cholinergics</subject><subject>Cloning</subject><subject>Clonorchis sinensis</subject><subject>Clonorchis sinensis - drug effects</subject><subject>Clonorchis sinensis - physiology</subject><subject>Dopamine</subject><subject>Dopamine - pharmacology</subject><subject>Dopamine D1 receptors</subject><subject>Dopamine D2 receptors</subject><subject>Dopamine D3 receptors</subject><subject>Dopamine Uptake Inhibitors - pharmacology</subject><subject>Duodenum</subject><subject>Excitatory Amino Acid Agents - pharmacology</subject><subject>Fasciola hepatica - drug effects</subject><subject>Fluke infections</subject><subject>Fluorenes - pharmacology</subject><subject>Fluorescent antibody technique</subject><subject>Fluoxetine</subject><subject>FMRFamide</subject><subject>FMRFamide - pharmacology</subject><subject>Glutamatergic transmission</subject><subject>Immunofluorescence</subject><subject>Immunomodulation</subject><subject>Inhibitors</subject><subject>Juveniles</subject><subject>Laboratory animals</subject><subject>Liver</subject><subject>Medicine</subject><subject>Migratory species</subject><subject>Minors</subject><subject>Modulators</subject><subject>Motility</subject><subject>Muscles</subject><subject>Nervous system</subject><subject>Neuromodulation</subject><subject>Neurons</subject><subject>Neuropeptide Y</subject><subject>Neuropeptide Y - 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pharmacology</topic><topic>Bile</topic><topic>Bile ducts</topic><topic>Biology</topic><topic>Biology and Life Sciences</topic><topic>Biphenyl Compounds - pharmacology</topic><topic>Care and treatment</topic><topic>Cell migration</topic><topic>Chemotaxis</topic><topic>Chemotaxis - drug effects</topic><topic>Chemotaxis - physiology</topic><topic>Cholic Acid</topic><topic>Cholinergics</topic><topic>Cloning</topic><topic>Clonorchis sinensis</topic><topic>Clonorchis sinensis - drug effects</topic><topic>Clonorchis sinensis - physiology</topic><topic>Dopamine</topic><topic>Dopamine - pharmacology</topic><topic>Dopamine D1 receptors</topic><topic>Dopamine D2 receptors</topic><topic>Dopamine D3 receptors</topic><topic>Dopamine Uptake Inhibitors - pharmacology</topic><topic>Duodenum</topic><topic>Excitatory Amino Acid Agents - pharmacology</topic><topic>Fasciola hepatica - drug effects</topic><topic>Fluke infections</topic><topic>Fluorenes - pharmacology</topic><topic>Fluorescent antibody technique</topic><topic>Fluoxetine</topic><topic>FMRFamide</topic><topic>FMRFamide - pharmacology</topic><topic>Glutamatergic transmission</topic><topic>Immunofluorescence</topic><topic>Immunomodulation</topic><topic>Inhibitors</topic><topic>Juveniles</topic><topic>Laboratory animals</topic><topic>Liver</topic><topic>Medicine</topic><topic>Migratory species</topic><topic>Minors</topic><topic>Modulators</topic><topic>Motility</topic><topic>Muscles</topic><topic>Nervous system</topic><topic>Neuromodulation</topic><topic>Neurons</topic><topic>Neuropeptide Y</topic><topic>Neuropeptide Y - pharmacology</topic><topic>Neuropeptides</topic><topic>Neurosciences</topic><topic>Neurotransmitter Agents - pharmacology</topic><topic>Parasites</topic><topic>Parasitology</topic><topic>Peptide YY - pharmacology</topic><topic>Peptides</topic><topic>Phenols (Class of compounds)</topic><topic>Physical Sciences</topic><topic>Piperazines - pharmacology</topic><topic>Pirenzepine</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Risk factors</topic><topic>Serotonin</topic><topic>Serotonin Agents - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS neglected tropical diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Shunyu</au><au>Song, Jin-Ho</au><au>Kim, Tae Im</au><au>Yoo, Won Gi</au><au>Won, Moo-Ho</au><au>Dai, Fuhong</au><au>Hong, Sung-Jong</au><au>Chai, Jong-Yil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemotactic migration of newly excysted juvenile Clonorchis sinensis is suppressed by neuro-antagonists</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2019-08-13</date><risdate>2019</risdate><volume>13</volume><issue>8</issue><spage>e0007573</spage><epage>e0007573</epage><pages>e0007573-e0007573</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>The metacercariae of the Clonorchis sinensis liver fluke excyst in the duodenum of mammalian hosts, and the newly excysted juveniles (CsNEJs) migrate along the bile duct via bile chemotaxis. Cholic acid is a major component of bile that induces this migration. We investigated the neuronal control of chemotactic behavior of CsNEJs toward cholic acid. The migration of CsNEJs was strongly inhibited at sub-micromolar concentration by dopamine D1 (LE-300 and SKF-83566), D2 (spiramide, nemonapride, and sulpiride), and D3 (GR-103691 and NGB-2904) receptor antagonists, as well as a dopamine reuptake inhibitor (BTCP). Neuropeptides, FMRFamide, peptide YY, and neuropeptide Y were also potent inhibitors of chemotaxis. Meanwhile, serotonergic, glutamatergic, and cholinergic inhibitors did not affect chemotaxis, with the exception of fluoxetine and CNQX. Confocal immunofluorescence analysis indicated that dopaminergic and cholinergic neurons were colocalized in the somatic muscle tissues of adult C. sinensis. Our findings suggest that dopaminergic neurons and neuropeptides play a major role in the chemotactic migration of CsNEJs to bile, and their inhibitors or modulators could be utilized to prevent their migration from the bile duct.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31408466</pmid><doi>10.1371/journal.pntd.0007573</doi><orcidid>https://orcid.org/0000-0002-8677-4714</orcidid><orcidid>https://orcid.org/0000-0002-3041-5560</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2019-08, Vol.13 (8), p.e0007573-e0007573 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Acids Animals Antidepressants Benzamides - pharmacology Bile Bile ducts Biology Biology and Life Sciences Biphenyl Compounds - pharmacology Care and treatment Cell migration Chemotaxis Chemotaxis - drug effects Chemotaxis - physiology Cholic Acid Cholinergics Cloning Clonorchis sinensis Clonorchis sinensis - drug effects Clonorchis sinensis - physiology Dopamine Dopamine - pharmacology Dopamine D1 receptors Dopamine D2 receptors Dopamine D3 receptors Dopamine Uptake Inhibitors - pharmacology Duodenum Excitatory Amino Acid Agents - pharmacology Fasciola hepatica - drug effects Fluke infections Fluorenes - pharmacology Fluorescent antibody technique Fluoxetine FMRFamide FMRFamide - pharmacology Glutamatergic transmission Immunofluorescence Immunomodulation Inhibitors Juveniles Laboratory animals Liver Medicine Migratory species Minors Modulators Motility Muscles Nervous system Neuromodulation Neurons Neuropeptide Y Neuropeptide Y - pharmacology Neuropeptides Neurosciences Neurotransmitter Agents - pharmacology Parasites Parasitology Peptide YY - pharmacology Peptides Phenols (Class of compounds) Physical Sciences Piperazines - pharmacology Pirenzepine Proteins Receptors Risk factors Serotonin Serotonin Agents - pharmacology Spiro Compounds - pharmacology Sulpiride Sulpiride - pharmacology Tropical diseases University colleges |
| title | Chemotactic migration of newly excysted juvenile Clonorchis sinensis is suppressed by neuro-antagonists |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T15%3A58%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Chemotactic%20migration%20of%20newly%20excysted%20juvenile%20Clonorchis%20sinensis%20is%20suppressed%20by%20neuro-antagonists&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Li,%20Shunyu&rft.date=2019-08-13&rft.volume=13&rft.issue=8&rft.spage=e0007573&rft.epage=e0007573&rft.pages=e0007573-e0007573&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0007573&rft_dat=%3Cgale_plos_%3EA598979014%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2291479750&rft_id=info:pmid/31408466&rft_galeid=A598979014&rft_doaj_id=oai_doaj_org_article_a053e51d64cf4a029b6d772c11910236&rfr_iscdi=true |