Evaluation of different detector types in measurement of ATP bioluminescence compared to colony counting method for measuring bacterial burden of hospital surfaces
The ATP bioluminescence method has been increasingly employed as a rapid, on-site detection method in nosocomial infections control. In this study, we used a paired design of monitoring methods, the colony counting method (C) and the ATP bioluminescence method, to evaluate environmental surfaces aft...
Gespeichert in:
| Veröffentlicht in: | PloS one 2019-09, Vol.14 (9), p.e0221665 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 9 |
| container_start_page | e0221665 |
| container_title | PloS one |
| container_volume | 14 |
| creator | Xu, Huiqiong Liang, Jiansheng Wang, Yimei Wang, Bin Zhang, Tianbao Liu, Xiaoli Gong, Lin |
| description | The ATP bioluminescence method has been increasingly employed as a rapid, on-site detection method in nosocomial infections control. In this study, we used a paired design of monitoring methods, the colony counting method (C) and the ATP bioluminescence method, to evaluate environmental surfaces after disinfection. The ATP bioluminescence method included three detector types (B, P, and N). Every surface after disinfection was performed by combining two types of monitoring methods or detectors. There was no statistically significant difference in theATP content per surface siteamong samples from intensive care units (ICUs)and internal medicine wards using B (p = 0.435) and P (p = 0.260). According to the Spearman's rank correlation coefficients, with the exception of the correlation between the ATP content values detected by B and P, the correlation between the values generated by the remaining methods/detectors was weak or lacking, whereasthe differences between the detectors were statistically significant. Therefore, there are differences between the ATP bioluminescence method and the colony counting method, also between different detectors. |
| doi_str_mv | 10.1371/journal.pone.0221665 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2285715885</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A598585289</galeid><doaj_id>oai_doaj_org_article_80f422debc9f4f8f8c349f77ea1330ac</doaj_id><sourcerecordid>A598585289</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-bbe60d18be559b3f7d11389b6427bfb667bbb96fe6ab5e0750cc33adb7a21b333</originalsourceid><addsrcrecordid>eNqNk9GK1DAUhoso7rr6BqIFQfBixqRp0_RGGJZVBxZWdPU2JOnJTJY2qUm6OM_ji5rZ6S5TUJBeJJx8_9_DzzlZ9hKjJSY1fn_jRm9FtxychSUqCkxp9Sg7xQ0pFrRA5PHR_SR7FsINQhVhlD7NTgguG9SU7DT7fXErulFE42zudN4arcGDjXkLEVR0Po-7AUJubN6DCKOHfv-a0NX1l1wa1429sRAUWAW5cv0gPLR5dOneObtLx2ijsZskj1vX5jpZHpz2RSlUBG9El8vRt3DXw9aFwcRUSowWCsLz7IkWXYAX03mWff94cX3-eXF59Wl9vrpcKNoUcSElUNRiJqGqGkl03WJMWCNpWdRSS0prKWVDNVAhK0B1hZQiRLSyFgWWhJCz7PXBd-hc4FO-gRcFq2pcMVYlYn0gWidu-OBNL_yOO2H4XcH5DRc-GtUBZ0iXRdGCVI0uNdNMkbLRdQ0CE4KESl4fpr-Nsoc2BRi96Gam8xdrtnzjbjmtCWoITgZvJgPvfo4Q4j9anqiNSF0Zq10yU70Jiq-qhlWsKliTqOVfqPS10BuVJkybVJ8J3s0EiYnwK27EGAJff_v6_-zVjzn79ojdgujiNqQh2w9omIPlAVTeheBBPySHEd8vyH0afL8gfFqQJHt1nPqD6H4jyB-KSRDh</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2285715885</pqid></control><display><type>article</type><title>Evaluation of different detector types in measurement of ATP bioluminescence compared to colony counting method for measuring bacterial burden of hospital surfaces</title><source>MEDLINE</source><source>Directory of Open Access Journals</source><source>Public Library of Science</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>EZB Electronic Journals Library</source><creator>Xu, Huiqiong ; Liang, Jiansheng ; Wang, Yimei ; Wang, Bin ; Zhang, Tianbao ; Liu, Xiaoli ; Gong, Lin</creator><contributor>Leekha, Surbhi</contributor><creatorcontrib>Xu, Huiqiong ; Liang, Jiansheng ; Wang, Yimei ; Wang, Bin ; Zhang, Tianbao ; Liu, Xiaoli ; Gong, Lin ; Leekha, Surbhi</creatorcontrib><description>The ATP bioluminescence method has been increasingly employed as a rapid, on-site detection method in nosocomial infections control. In this study, we used a paired design of monitoring methods, the colony counting method (C) and the ATP bioluminescence method, to evaluate environmental surfaces after disinfection. The ATP bioluminescence method included three detector types (B, P, and N). Every surface after disinfection was performed by combining two types of monitoring methods or detectors. There was no statistically significant difference in theATP content per surface siteamong samples from intensive care units (ICUs)and internal medicine wards using B (p = 0.435) and P (p = 0.260). According to the Spearman's rank correlation coefficients, with the exception of the correlation between the ATP content values detected by B and P, the correlation between the values generated by the remaining methods/detectors was weak or lacking, whereasthe differences between the detectors were statistically significant. Therefore, there are differences between the ATP bioluminescence method and the colony counting method, also between different detectors.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0221665</identifier><identifier>PMID: 31490948</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenosine triphosphate ; Adenosine Triphosphate - metabolism ; ATP ; Bacteria - isolation & purification ; Bacteria - metabolism ; Bacterial Physiological Phenomena ; Biology and Life Sciences ; Bioluminescence ; Care and treatment ; Colonies ; Colony Count, Microbial ; Community health care ; Correlation ; Correlation coefficient ; Correlation coefficients ; Cross infection ; Detection equipment ; Detectors ; Disease control ; Disease prevention ; Disinfection ; Disinfection & disinfectants ; Gram-positive bacteria ; Health services ; Hospitals ; Humans ; Infection ; Infection control ; Intensive care ; Intensive care units ; Internal medicine ; Luminescent Measurements ; Medical equipment ; Medical research ; Medicine ; Medicine and Health Sciences ; Methods ; Microorganisms ; Monitoring ; Monitoring methods ; Nosocomial infection ; Nosocomial infections ; Patient care ; Pest control ; Physical Sciences ; Research and Analysis Methods ; Safety and security measures ; Sensors ; Statistical analysis ; Statistical methods ; Statistical significance</subject><ispartof>PloS one, 2019-09, Vol.14 (9), p.e0221665</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Xu et al 2019 Xu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-bbe60d18be559b3f7d11389b6427bfb667bbb96fe6ab5e0750cc33adb7a21b333</citedby><cites>FETCH-LOGICAL-c692t-bbe60d18be559b3f7d11389b6427bfb667bbb96fe6ab5e0750cc33adb7a21b333</cites><orcidid>0000-0001-5001-2825</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6730931/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6730931/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31490948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Leekha, Surbhi</contributor><creatorcontrib>Xu, Huiqiong</creatorcontrib><creatorcontrib>Liang, Jiansheng</creatorcontrib><creatorcontrib>Wang, Yimei</creatorcontrib><creatorcontrib>Wang, Bin</creatorcontrib><creatorcontrib>Zhang, Tianbao</creatorcontrib><creatorcontrib>Liu, Xiaoli</creatorcontrib><creatorcontrib>Gong, Lin</creatorcontrib><title>Evaluation of different detector types in measurement of ATP bioluminescence compared to colony counting method for measuring bacterial burden of hospital surfaces</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The ATP bioluminescence method has been increasingly employed as a rapid, on-site detection method in nosocomial infections control. In this study, we used a paired design of monitoring methods, the colony counting method (C) and the ATP bioluminescence method, to evaluate environmental surfaces after disinfection. The ATP bioluminescence method included three detector types (B, P, and N). Every surface after disinfection was performed by combining two types of monitoring methods or detectors. There was no statistically significant difference in theATP content per surface siteamong samples from intensive care units (ICUs)and internal medicine wards using B (p = 0.435) and P (p = 0.260). According to the Spearman's rank correlation coefficients, with the exception of the correlation between the ATP content values detected by B and P, the correlation between the values generated by the remaining methods/detectors was weak or lacking, whereasthe differences between the detectors were statistically significant. Therefore, there are differences between the ATP bioluminescence method and the colony counting method, also between different detectors.</description><subject>Adenosine triphosphate</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>ATP</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteria - metabolism</subject><subject>Bacterial Physiological Phenomena</subject><subject>Biology and Life Sciences</subject><subject>Bioluminescence</subject><subject>Care and treatment</subject><subject>Colonies</subject><subject>Colony Count, Microbial</subject><subject>Community health care</subject><subject>Correlation</subject><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Cross infection</subject><subject>Detection equipment</subject><subject>Detectors</subject><subject>Disease control</subject><subject>Disease prevention</subject><subject>Disinfection</subject><subject>Disinfection & disinfectants</subject><subject>Gram-positive bacteria</subject><subject>Health services</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Infection</subject><subject>Infection control</subject><subject>Intensive care</subject><subject>Intensive care units</subject><subject>Internal medicine</subject><subject>Luminescent Measurements</subject><subject>Medical equipment</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Microorganisms</subject><subject>Monitoring</subject><subject>Monitoring methods</subject><subject>Nosocomial infection</subject><subject>Nosocomial infections</subject><subject>Patient care</subject><subject>Pest control</subject><subject>Physical Sciences</subject><subject>Research and Analysis Methods</subject><subject>Safety and security measures</subject><subject>Sensors</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>Statistical significance</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9GK1DAUhoso7rr6BqIFQfBixqRp0_RGGJZVBxZWdPU2JOnJTJY2qUm6OM_ji5rZ6S5TUJBeJJx8_9_DzzlZ9hKjJSY1fn_jRm9FtxychSUqCkxp9Sg7xQ0pFrRA5PHR_SR7FsINQhVhlD7NTgguG9SU7DT7fXErulFE42zudN4arcGDjXkLEVR0Po-7AUJubN6DCKOHfv-a0NX1l1wa1429sRAUWAW5cv0gPLR5dOneObtLx2ijsZskj1vX5jpZHpz2RSlUBG9El8vRt3DXw9aFwcRUSowWCsLz7IkWXYAX03mWff94cX3-eXF59Wl9vrpcKNoUcSElUNRiJqGqGkl03WJMWCNpWdRSS0prKWVDNVAhK0B1hZQiRLSyFgWWhJCz7PXBd-hc4FO-gRcFq2pcMVYlYn0gWidu-OBNL_yOO2H4XcH5DRc-GtUBZ0iXRdGCVI0uNdNMkbLRdQ0CE4KESl4fpr-Nsoc2BRi96Gam8xdrtnzjbjmtCWoITgZvJgPvfo4Q4j9anqiNSF0Zq10yU70Jiq-qhlWsKliTqOVfqPS10BuVJkybVJ8J3s0EiYnwK27EGAJff_v6_-zVjzn79ojdgujiNqQh2w9omIPlAVTeheBBPySHEd8vyH0afL8gfFqQJHt1nPqD6H4jyB-KSRDh</recordid><startdate>20190906</startdate><enddate>20190906</enddate><creator>Xu, Huiqiong</creator><creator>Liang, Jiansheng</creator><creator>Wang, Yimei</creator><creator>Wang, Bin</creator><creator>Zhang, Tianbao</creator><creator>Liu, Xiaoli</creator><creator>Gong, Lin</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5001-2825</orcidid></search><sort><creationdate>20190906</creationdate><title>Evaluation of different detector types in measurement of ATP bioluminescence compared to colony counting method for measuring bacterial burden of hospital surfaces</title><author>Xu, Huiqiong ; Liang, Jiansheng ; Wang, Yimei ; Wang, Bin ; Zhang, Tianbao ; Liu, Xiaoli ; Gong, Lin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-bbe60d18be559b3f7d11389b6427bfb667bbb96fe6ab5e0750cc33adb7a21b333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adenosine triphosphate</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>ATP</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteria - metabolism</topic><topic>Bacterial Physiological Phenomena</topic><topic>Biology and Life Sciences</topic><topic>Bioluminescence</topic><topic>Care and treatment</topic><topic>Colonies</topic><topic>Colony Count, Microbial</topic><topic>Community health care</topic><topic>Correlation</topic><topic>Correlation coefficient</topic><topic>Correlation coefficients</topic><topic>Cross infection</topic><topic>Detection equipment</topic><topic>Detectors</topic><topic>Disease control</topic><topic>Disease prevention</topic><topic>Disinfection</topic><topic>Disinfection & disinfectants</topic><topic>Gram-positive bacteria</topic><topic>Health services</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Infection</topic><topic>Infection control</topic><topic>Intensive care</topic><topic>Intensive care units</topic><topic>Internal medicine</topic><topic>Luminescent Measurements</topic><topic>Medical equipment</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Methods</topic><topic>Microorganisms</topic><topic>Monitoring</topic><topic>Monitoring methods</topic><topic>Nosocomial infection</topic><topic>Nosocomial infections</topic><topic>Patient care</topic><topic>Pest control</topic><topic>Physical Sciences</topic><topic>Research and Analysis Methods</topic><topic>Safety and security measures</topic><topic>Sensors</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><topic>Statistical significance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Huiqiong</creatorcontrib><creatorcontrib>Liang, Jiansheng</creatorcontrib><creatorcontrib>Wang, Yimei</creatorcontrib><creatorcontrib>Wang, Bin</creatorcontrib><creatorcontrib>Zhang, Tianbao</creatorcontrib><creatorcontrib>Liu, Xiaoli</creatorcontrib><creatorcontrib>Gong, Lin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints In Context</collection><collection>Gale in Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Database (1962 - current)</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>https://resources.nclive.org/materials</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Huiqiong</au><au>Liang, Jiansheng</au><au>Wang, Yimei</au><au>Wang, Bin</au><au>Zhang, Tianbao</au><au>Liu, Xiaoli</au><au>Gong, Lin</au><au>Leekha, Surbhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of different detector types in measurement of ATP bioluminescence compared to colony counting method for measuring bacterial burden of hospital surfaces</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-09-06</date><risdate>2019</risdate><volume>14</volume><issue>9</issue><spage>e0221665</spage><pages>e0221665-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The ATP bioluminescence method has been increasingly employed as a rapid, on-site detection method in nosocomial infections control. In this study, we used a paired design of monitoring methods, the colony counting method (C) and the ATP bioluminescence method, to evaluate environmental surfaces after disinfection. The ATP bioluminescence method included three detector types (B, P, and N). Every surface after disinfection was performed by combining two types of monitoring methods or detectors. There was no statistically significant difference in theATP content per surface siteamong samples from intensive care units (ICUs)and internal medicine wards using B (p = 0.435) and P (p = 0.260). According to the Spearman's rank correlation coefficients, with the exception of the correlation between the ATP content values detected by B and P, the correlation between the values generated by the remaining methods/detectors was weak or lacking, whereasthe differences between the detectors were statistically significant. Therefore, there are differences between the ATP bioluminescence method and the colony counting method, also between different detectors.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31490948</pmid><doi>10.1371/journal.pone.0221665</doi><tpages>e0221665</tpages><orcidid>https://orcid.org/0000-0001-5001-2825</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-09, Vol.14 (9), p.e0221665 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2285715885 |
| source | MEDLINE; Directory of Open Access Journals; Public Library of Science; PubMed Central; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Adenosine triphosphate Adenosine Triphosphate - metabolism ATP Bacteria - isolation & purification Bacteria - metabolism Bacterial Physiological Phenomena Biology and Life Sciences Bioluminescence Care and treatment Colonies Colony Count, Microbial Community health care Correlation Correlation coefficient Correlation coefficients Cross infection Detection equipment Detectors Disease control Disease prevention Disinfection Disinfection & disinfectants Gram-positive bacteria Health services Hospitals Humans Infection Infection control Intensive care Intensive care units Internal medicine Luminescent Measurements Medical equipment Medical research Medicine Medicine and Health Sciences Methods Microorganisms Monitoring Monitoring methods Nosocomial infection Nosocomial infections Patient care Pest control Physical Sciences Research and Analysis Methods Safety and security measures Sensors Statistical analysis Statistical methods Statistical significance |
| title | Evaluation of different detector types in measurement of ATP bioluminescence compared to colony counting method for measuring bacterial burden of hospital surfaces |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T16%3A35%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20different%20detector%20types%20in%20measurement%20of%20ATP%20bioluminescence%20compared%20to%20colony%20counting%20method%20for%20measuring%20bacterial%20burden%20of%20hospital%20surfaces&rft.jtitle=PloS%20one&rft.au=Xu,%20Huiqiong&rft.date=2019-09-06&rft.volume=14&rft.issue=9&rft.spage=e0221665&rft.pages=e0221665-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0221665&rft_dat=%3Cgale_plos_%3EA598585289%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2285715885&rft_id=info:pmid/31490948&rft_galeid=A598585289&rft_doaj_id=oai_doaj_org_article_80f422debc9f4f8f8c349f77ea1330ac&rfr_iscdi=true |