Clinical correlation of influenza and respiratory syncytial virus load measured by digital PCR
Acute respiratory tract infections are a major cause of respiratory morbidity and mortality in pediatric patients worldwide. However, accurate viral and immunologic markers to predict clinical outcomes of this patient population are still lacking. Droplet digital PCR assays for influenza and respira...
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| Veröffentlicht in: | PloS one 2019-09, Vol.14 (9), p.e0220908-e0220908 |
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| creator | Hijano, Diego R Brazelton de Cardenas, Jessica Maron, Gabriela Garner, Cherilyn D Ferrolino, Jose A Dallas, Ronald H Gu, Zhengming Hayden, Randall T |
| description | Acute respiratory tract infections are a major cause of respiratory morbidity and mortality in pediatric patients worldwide. However, accurate viral and immunologic markers to predict clinical outcomes of this patient population are still lacking. Droplet digital PCR assays for influenza and respiratory syncytial virus (RSV) were designed and performed in 64 respiratory samples from 23 patients with influenza virus infection and 73 samples from 19 patients with RSV infection. Samples of patients with hematologic malignancies, solid tumors, or sickle cell disease were included. Clinical information from institutional medical records was reviewed to assess disease severity. Samples from patients with fever or respiratory symptoms had a significantly higher viral loads than those from asymptomatic patients. Samples from patients with influenza virus and RSV infection collected at presentation had significantly higher viral loads than those collected from patients after completing a course of oseltamivir or ribavirin, respectively. RSV loads correlated positively with clinical symptoms in patients ≤5 years of age, whereas influenza viral loads were associated with clinical symptoms, irrespective of age. Patients receiving antivirals for influenza and RSV had a significant reduction in viral loads after completing therapy. Digital PCR offers an effective method to monitor the efficacy of antiviral treatment for respiratory tract infections in immunocompromised hosts. |
| doi_str_mv | 10.1371/journal.pone.0220908 |
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However, accurate viral and immunologic markers to predict clinical outcomes of this patient population are still lacking. Droplet digital PCR assays for influenza and respiratory syncytial virus (RSV) were designed and performed in 64 respiratory samples from 23 patients with influenza virus infection and 73 samples from 19 patients with RSV infection. Samples of patients with hematologic malignancies, solid tumors, or sickle cell disease were included. Clinical information from institutional medical records was reviewed to assess disease severity. Samples from patients with fever or respiratory symptoms had a significantly higher viral loads than those from asymptomatic patients. Samples from patients with influenza virus and RSV infection collected at presentation had significantly higher viral loads than those collected from patients after completing a course of oseltamivir or ribavirin, respectively. RSV loads correlated positively with clinical symptoms in patients ≤5 years of age, whereas influenza viral loads were associated with clinical symptoms, irrespective of age. Patients receiving antivirals for influenza and RSV had a significant reduction in viral loads after completing therapy. Digital PCR offers an effective method to monitor the efficacy of antiviral treatment for respiratory tract infections in immunocompromised hosts.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0220908</identifier><identifier>PMID: 31479459</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Antiviral agents ; Antiviral Agents - therapeutic use ; Biology and Life Sciences ; Blood cancer ; Child ; Child health ; Child, Preschool ; Children & youth ; Clinical outcomes ; Coinfection ; Cytomegalovirus ; Drug resistance ; Female ; Fever ; Hospitals ; Humans ; Immunocompromised hosts ; Infant ; Infection ; Infections ; Infectious diseases ; Influenza ; Influenza A virus - genetics ; Influenza B virus - genetics ; Influenza viruses ; Influenza, Human - diagnosis ; Influenza, Human - drug therapy ; Influenza, Human - virology ; Lymphopenia - diagnosis ; Lymphopenia - etiology ; Male ; Measurement ; Medical records ; Medical research ; Medicine and Health Sciences ; Methods ; Monitoring methods ; Morbidity ; Mortality ; Oseltamivir ; Oseltamivir phosphate ; Pathology ; Patients ; Pediatrics ; Physiological aspects ; Polymerase chain reaction ; Real-Time Polymerase Chain Reaction ; Reproducibility of Results ; Research and Analysis Methods ; Respiratory symptoms ; Respiratory syncytial virus ; Respiratory Syncytial Virus Infections - diagnosis ; Respiratory Syncytial Virus Infections - drug therapy ; Respiratory Syncytial Virus Infections - virology ; Respiratory Syncytial Virus, Human - genetics ; Respiratory system agents ; Respiratory tract ; Respiratory tract diseases ; Ribavirin ; Sickle cell anemia ; Sickle cell disease ; Signs and symptoms ; Solid tumors ; Symptom Assessment ; Treatment Outcome ; Tumors ; Viral infections ; Viral Load ; Virus diseases ; Viruses ; Young Adult</subject><ispartof>PloS one, 2019-09, Vol.14 (9), p.e0220908-e0220908</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Hijano et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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However, accurate viral and immunologic markers to predict clinical outcomes of this patient population are still lacking. Droplet digital PCR assays for influenza and respiratory syncytial virus (RSV) were designed and performed in 64 respiratory samples from 23 patients with influenza virus infection and 73 samples from 19 patients with RSV infection. Samples of patients with hematologic malignancies, solid tumors, or sickle cell disease were included. Clinical information from institutional medical records was reviewed to assess disease severity. Samples from patients with fever or respiratory symptoms had a significantly higher viral loads than those from asymptomatic patients. Samples from patients with influenza virus and RSV infection collected at presentation had significantly higher viral loads than those collected from patients after completing a course of oseltamivir or ribavirin, respectively. 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diagnosis</subject><subject>Influenza, Human - drug therapy</subject><subject>Influenza, Human - virology</subject><subject>Lymphopenia - diagnosis</subject><subject>Lymphopenia - etiology</subject><subject>Male</subject><subject>Measurement</subject><subject>Medical records</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Monitoring methods</subject><subject>Morbidity</subject><subject>Mortality</subject><subject>Oseltamivir</subject><subject>Oseltamivir phosphate</subject><subject>Pathology</subject><subject>Patients</subject><subject>Pediatrics</subject><subject>Physiological aspects</subject><subject>Polymerase chain reaction</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reproducibility of Results</subject><subject>Research and Analysis Methods</subject><subject>Respiratory symptoms</subject><subject>Respiratory syncytial virus</subject><subject>Respiratory Syncytial Virus Infections - diagnosis</subject><subject>Respiratory Syncytial Virus Infections - drug therapy</subject><subject>Respiratory Syncytial Virus Infections - virology</subject><subject>Respiratory Syncytial Virus, Human - genetics</subject><subject>Respiratory system agents</subject><subject>Respiratory tract</subject><subject>Respiratory tract diseases</subject><subject>Ribavirin</subject><subject>Sickle cell anemia</subject><subject>Sickle cell disease</subject><subject>Signs and symptoms</subject><subject>Solid tumors</subject><subject>Symptom Assessment</subject><subject>Treatment Outcome</subject><subject>Tumors</subject><subject>Viral infections</subject><subject>Viral Load</subject><subject>Virus diseases</subject><subject>Viruses</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk0tvEzEQx1cIREvgGyBYCQnBIcHPzfqCVEU8IlUqKo8j1qx3NnHlrIO9WxE-PU6zrbKoB-yDLfv3n_GMZ7LsOSUzyuf03ZXvQwtutvUtzghjRJHyQXZKFWfTghH-8Gh_kj2J8YoQycuieJydcCrmSkh1mv1cONtaAy43PgR00Fnf5r7Jbdu4Hts_kENb5wHj1gbofNjlcdeaXWeT5NqGPubOQ51vEGIfsM6rXV7ble3S9ZfF5dPsUQMu4rNhnWTfP374tvg8Pb_4tFycnU_NXJbdlBa1rOeqAiUEN6xpUBIhKlWIIoWqRIlYNkgrAkCJbBpGKQFRAOcVQSNKPsleHuxunY96SE3UjJVcScFYkYjlgag9XOltsBsIO-3B6psDH1YaQmeNQ02lLEtkpSEURa0qRZhABXUpOFY8jUn2fvDWVxusDbZdADcyOr5p7Vqv_LUu5owQtn_um8FA8L96jJ3e2GjQOWjR9zfvFlIKQmhCX_2D3h_dQK0gBZD-zie_Zm9Un0lVskLOpUzU7B4qzRo31qQ6amw6HwnejgSJ6fB3t4I-Rr38evn_7MWPMfv6iF0juG4dvev3xRfHoDiAJvgYAzZ3SaZE79vgNht63wZ6aIMke3H8QXei27rnfwG7wgI6</recordid><startdate>20190903</startdate><enddate>20190903</enddate><creator>Hijano, Diego R</creator><creator>Brazelton de Cardenas, Jessica</creator><creator>Maron, Gabriela</creator><creator>Garner, Cherilyn D</creator><creator>Ferrolino, Jose A</creator><creator>Dallas, Ronald H</creator><creator>Gu, Zhengming</creator><creator>Hayden, Randall T</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>COVID</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3259-6801</orcidid><orcidid>https://orcid.org/0000-0002-2903-666X</orcidid></search><sort><creationdate>20190903</creationdate><title>Clinical correlation of influenza and respiratory syncytial virus load measured by digital PCR</title><author>Hijano, Diego R ; Brazelton de Cardenas, Jessica ; Maron, Gabriela ; Garner, Cherilyn D ; Ferrolino, Jose A ; Dallas, Ronald H ; Gu, Zhengming ; Hayden, Randall T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-16d5d79ba9443c2ffe5044b9646371948ee8fe1b0aa105ff2110a46a33b0ec483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adolescent</topic><topic>Antiviral agents</topic><topic>Antiviral Agents - 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However, accurate viral and immunologic markers to predict clinical outcomes of this patient population are still lacking. Droplet digital PCR assays for influenza and respiratory syncytial virus (RSV) were designed and performed in 64 respiratory samples from 23 patients with influenza virus infection and 73 samples from 19 patients with RSV infection. Samples of patients with hematologic malignancies, solid tumors, or sickle cell disease were included. Clinical information from institutional medical records was reviewed to assess disease severity. Samples from patients with fever or respiratory symptoms had a significantly higher viral loads than those from asymptomatic patients. Samples from patients with influenza virus and RSV infection collected at presentation had significantly higher viral loads than those collected from patients after completing a course of oseltamivir or ribavirin, respectively. RSV loads correlated positively with clinical symptoms in patients ≤5 years of age, whereas influenza viral loads were associated with clinical symptoms, irrespective of age. Patients receiving antivirals for influenza and RSV had a significant reduction in viral loads after completing therapy. Digital PCR offers an effective method to monitor the efficacy of antiviral treatment for respiratory tract infections in immunocompromised hosts.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31479459</pmid><doi>10.1371/journal.pone.0220908</doi><tpages>e0220908</tpages><orcidid>https://orcid.org/0000-0002-3259-6801</orcidid><orcidid>https://orcid.org/0000-0002-2903-666X</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_2283954226 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Adolescent Antiviral agents Antiviral Agents - therapeutic use Biology and Life Sciences Blood cancer Child Child health Child, Preschool Children & youth Clinical outcomes Coinfection Cytomegalovirus Drug resistance Female Fever Hospitals Humans Immunocompromised hosts Infant Infection Infections Infectious diseases Influenza Influenza A virus - genetics Influenza B virus - genetics Influenza viruses Influenza, Human - diagnosis Influenza, Human - drug therapy Influenza, Human - virology Lymphopenia - diagnosis Lymphopenia - etiology Male Measurement Medical records Medical research Medicine and Health Sciences Methods Monitoring methods Morbidity Mortality Oseltamivir Oseltamivir phosphate Pathology Patients Pediatrics Physiological aspects Polymerase chain reaction Real-Time Polymerase Chain Reaction Reproducibility of Results Research and Analysis Methods Respiratory symptoms Respiratory syncytial virus Respiratory Syncytial Virus Infections - diagnosis Respiratory Syncytial Virus Infections - drug therapy Respiratory Syncytial Virus Infections - virology Respiratory Syncytial Virus, Human - genetics Respiratory system agents Respiratory tract Respiratory tract diseases Ribavirin Sickle cell anemia Sickle cell disease Signs and symptoms Solid tumors Symptom Assessment Treatment Outcome Tumors Viral infections Viral Load Virus diseases Viruses Young Adult |
| title | Clinical correlation of influenza and respiratory syncytial virus load measured by digital PCR |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T03%3A41%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clinical%20correlation%20of%20influenza%20and%20respiratory%20syncytial%20virus%20load%20measured%20by%20digital%20PCR&rft.jtitle=PloS%20one&rft.au=Hijano,%20Diego%20R&rft.date=2019-09-03&rft.volume=14&rft.issue=9&rft.spage=e0220908&rft.epage=e0220908&rft.pages=e0220908-e0220908&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0220908&rft_dat=%3Cgale_plos_%3EA598265755%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2283954226&rft_id=info:pmid/31479459&rft_galeid=A598265755&rft_doaj_id=oai_doaj_org_article_15588e28c01e4d9b9024e9ad843eb333&rfr_iscdi=true |