Bacterial cellulose membrane functionalized with hydroxiapatite and anti-bone morphogenetic protein 2: A promising material for bone regeneration
Bone tissue engineering seeks to adequately restore functions related to physical and biological properties, aiming at a repair process similar to natural bone. The use of compatible biopolymers, such as bacterial cellulose (BC), as well as having interesting mechanical characteristics, presents a s...
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| Veröffentlicht in: | PloS one 2019-08, Vol.14 (8), p.e0221286 |
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| creator | Coelho, Fernanda Cavicchioli, Maurício Specian, Sybele Saska Scarel-Caminaga, Raquel Mantuaneli Penteado, Letícia de Aquino Medeiros, Alexandra Ivo de Ribeiro, Sidney José de Lima Capote, Ticiana Sidorenko de Oliveira |
| description | Bone tissue engineering seeks to adequately restore functions related to physical and biological properties, aiming at a repair process similar to natural bone. The use of compatible biopolymers, such as bacterial cellulose (BC), as well as having interesting mechanical characteristics, presents a slow in vivo degradation rate, and the ability to be chemically modified. To promote better bioactivity towards BC, we synthesized an innovative BC membrane associated to hydroxyapatite (HA) and anti-bone morphogenetic protein antibody (anti-BMP-2) (BC-HA-anti-BMP-2). We present the physical-chemical, biological and toxicological characterization of BC-HA-anti-BMP-2. Presence of BC and HA components in the membranes was confirmed by SEM-EDS and FTIR assays. No toxic potential was found in MC3T3-E1 cells by cytotoxicity assays (XTT Assay and Clonogenic Survival), genotoxicity (Comet Assay) and mutagenicity (Cytokinesis-blocked micronucleus Test). The in vitro release kinetics of anti-BMP-2 antibodies detected gradually reducing antibody levels, reducing approximately 70% in 7 days and 90% in 14 days. BC-HA-anti-BMP-2 increased SPP1, BGLAP, VEGF, ALPL, RUNX2 and TNFRSF11B expression, genes involved in bone repair and also increased mineralization nodules and phosphatase alcalin (ALP) activity levels. In conclusion, we developed BC-HA-anti-BMP-2 as an innovative and promising biomaterial with interesting physical-chemical and biological properties which may be a good alternative to treatment with commercial BMP-2 protein. |
| doi_str_mv | 10.1371/journal.pone.0221286 |
| format | Article |
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The use of compatible biopolymers, such as bacterial cellulose (BC), as well as having interesting mechanical characteristics, presents a slow in vivo degradation rate, and the ability to be chemically modified. To promote better bioactivity towards BC, we synthesized an innovative BC membrane associated to hydroxyapatite (HA) and anti-bone morphogenetic protein antibody (anti-BMP-2) (BC-HA-anti-BMP-2). We present the physical-chemical, biological and toxicological characterization of BC-HA-anti-BMP-2. Presence of BC and HA components in the membranes was confirmed by SEM-EDS and FTIR assays. No toxic potential was found in MC3T3-E1 cells by cytotoxicity assays (XTT Assay and Clonogenic Survival), genotoxicity (Comet Assay) and mutagenicity (Cytokinesis-blocked micronucleus Test). The in vitro release kinetics of anti-BMP-2 antibodies detected gradually reducing antibody levels, reducing approximately 70% in 7 days and 90% in 14 days. BC-HA-anti-BMP-2 increased SPP1, BGLAP, VEGF, ALPL, RUNX2 and TNFRSF11B expression, genes involved in bone repair and also increased mineralization nodules and phosphatase alcalin (ALP) activity levels. In conclusion, we developed BC-HA-anti-BMP-2 as an innovative and promising biomaterial with interesting physical-chemical and biological properties which may be a good alternative to treatment with commercial BMP-2 protein.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0221286</identifier><identifier>PMID: 31425530</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Anti-antibodies ; Antibodies ; Antibodies, Immobilized - immunology ; Antibodies, Immobilized - pharmacology ; Antibodies, Monoclonal - immunology ; Antibodies, Monoclonal - pharmacology ; Bacterial proteins ; Bioassays ; Biocompatibility ; Biodegradation ; Biological activity ; Biological products ; Biological properties ; Biology and Life Sciences ; Biomaterials ; Biomedical materials ; Biopolymers ; Bone growth ; Bone healing ; Bone morphogenetic protein 2 ; Bone Morphogenetic Protein 2 - immunology ; Bone Morphogenetic Protein 2 - metabolism ; Bone morphogenetic proteins ; Bone regeneration ; Bone Regeneration - drug effects ; Bone Substitutes - chemistry ; Bone Substitutes - pharmacology ; Bones ; Calcium phosphates ; Cbfa-1 protein ; Cell adhesion & migration ; Cell Differentiation - drug effects ; Cell Line ; Cell survival ; Cellulose ; Cellulose - chemistry ; Cellulose - pharmacology ; Chemistry ; Comet assay ; Cytokinesis ; Cytotoxicity ; Damage detection ; Dentistry ; Durapatite - chemistry ; Durapatite - pharmacology ; Endothelial growth factors ; Engineering and Technology ; Gene expression ; Genes ; Genotoxicity ; Gluconacetobacter xylinus - chemistry ; Health aspects ; Hydroxyapatite ; Hydroxyapatites ; In vitro methods and tests ; In vivo methods and tests ; Inorganic chemistry ; Kinetics ; Materials ; Materials Testing ; Mechanical properties ; Medical research ; Medicine and Health Sciences ; Membranes ; Methods ; Mice ; Mineralization ; Morphology ; Mutagenicity ; Nanocomposites ; Nodules ; Organic chemistry ; Osteoblasts ; Osteogenesis - drug effects ; Osteoprotegerin ; Peptides ; Pharmaceutical sciences ; Phosphatases ; Physical Sciences ; Polymers ; Polysaccharides ; Proteins ; Regeneration ; Regeneration (physiology) ; Repair ; Signal transduction ; Signal Transduction - drug effects ; Stem cells ; Surgical implants ; Testing ; Tissue engineering ; Tissue Engineering - methods ; Toxicity ; Toxicology ; Trauma ; Vascular endothelial growth factor</subject><ispartof>PloS one, 2019-08, Vol.14 (8), p.e0221286</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Coelho et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Coelho et al 2019 Coelho et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-5bfb027c0adaa27bedc82ac230f735afb1a63e98ff339c376a2feb6090bed7063</citedby><cites>FETCH-LOGICAL-c692t-5bfb027c0adaa27bedc82ac230f735afb1a63e98ff339c376a2feb6090bed7063</cites><orcidid>0000-0001-5794-4174</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6699690/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6699690/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31425530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coelho, Fernanda</creatorcontrib><creatorcontrib>Cavicchioli, Maurício</creatorcontrib><creatorcontrib>Specian, Sybele Saska</creatorcontrib><creatorcontrib>Scarel-Caminaga, Raquel Mantuaneli</creatorcontrib><creatorcontrib>Penteado, Letícia de Aquino</creatorcontrib><creatorcontrib>Medeiros, Alexandra Ivo de</creatorcontrib><creatorcontrib>Ribeiro, Sidney José de Lima</creatorcontrib><creatorcontrib>Capote, Ticiana Sidorenko de Oliveira</creatorcontrib><title>Bacterial cellulose membrane functionalized with hydroxiapatite and anti-bone morphogenetic protein 2: A promising material for bone regeneration</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Bone tissue engineering seeks to adequately restore functions related to physical and biological properties, aiming at a repair process similar to natural bone. The use of compatible biopolymers, such as bacterial cellulose (BC), as well as having interesting mechanical characteristics, presents a slow in vivo degradation rate, and the ability to be chemically modified. To promote better bioactivity towards BC, we synthesized an innovative BC membrane associated to hydroxyapatite (HA) and anti-bone morphogenetic protein antibody (anti-BMP-2) (BC-HA-anti-BMP-2). We present the physical-chemical, biological and toxicological characterization of BC-HA-anti-BMP-2. Presence of BC and HA components in the membranes was confirmed by SEM-EDS and FTIR assays. No toxic potential was found in MC3T3-E1 cells by cytotoxicity assays (XTT Assay and Clonogenic Survival), genotoxicity (Comet Assay) and mutagenicity (Cytokinesis-blocked micronucleus Test). The in vitro release kinetics of anti-BMP-2 antibodies detected gradually reducing antibody levels, reducing approximately 70% in 7 days and 90% in 14 days. BC-HA-anti-BMP-2 increased SPP1, BGLAP, VEGF, ALPL, RUNX2 and TNFRSF11B expression, genes involved in bone repair and also increased mineralization nodules and phosphatase alcalin (ALP) activity levels. In conclusion, we developed BC-HA-anti-BMP-2 as an innovative and promising biomaterial with interesting physical-chemical and biological properties which may be a good alternative to treatment with commercial BMP-2 protein.</description><subject>Animals</subject><subject>Anti-antibodies</subject><subject>Antibodies</subject><subject>Antibodies, Immobilized - immunology</subject><subject>Antibodies, Immobilized - pharmacology</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Bacterial proteins</subject><subject>Bioassays</subject><subject>Biocompatibility</subject><subject>Biodegradation</subject><subject>Biological activity</subject><subject>Biological products</subject><subject>Biological properties</subject><subject>Biology and Life Sciences</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Biopolymers</subject><subject>Bone growth</subject><subject>Bone healing</subject><subject>Bone morphogenetic protein 2</subject><subject>Bone Morphogenetic Protein 2 - immunology</subject><subject>Bone Morphogenetic Protein 2 - metabolism</subject><subject>Bone morphogenetic proteins</subject><subject>Bone regeneration</subject><subject>Bone Regeneration - drug effects</subject><subject>Bone Substitutes - chemistry</subject><subject>Bone Substitutes - pharmacology</subject><subject>Bones</subject><subject>Calcium phosphates</subject><subject>Cbfa-1 protein</subject><subject>Cell adhesion & migration</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>Cell survival</subject><subject>Cellulose</subject><subject>Cellulose - chemistry</subject><subject>Cellulose - pharmacology</subject><subject>Chemistry</subject><subject>Comet assay</subject><subject>Cytokinesis</subject><subject>Cytotoxicity</subject><subject>Damage detection</subject><subject>Dentistry</subject><subject>Durapatite - chemistry</subject><subject>Durapatite - pharmacology</subject><subject>Endothelial growth factors</subject><subject>Engineering and Technology</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genotoxicity</subject><subject>Gluconacetobacter xylinus - chemistry</subject><subject>Health aspects</subject><subject>Hydroxyapatite</subject><subject>Hydroxyapatites</subject><subject>In vitro methods and tests</subject><subject>In vivo methods and tests</subject><subject>Inorganic chemistry</subject><subject>Kinetics</subject><subject>Materials</subject><subject>Materials Testing</subject><subject>Mechanical properties</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Membranes</subject><subject>Methods</subject><subject>Mice</subject><subject>Mineralization</subject><subject>Morphology</subject><subject>Mutagenicity</subject><subject>Nanocomposites</subject><subject>Nodules</subject><subject>Organic chemistry</subject><subject>Osteoblasts</subject><subject>Osteogenesis - drug effects</subject><subject>Osteoprotegerin</subject><subject>Peptides</subject><subject>Pharmaceutical sciences</subject><subject>Phosphatases</subject><subject>Physical Sciences</subject><subject>Polymers</subject><subject>Polysaccharides</subject><subject>Proteins</subject><subject>Regeneration</subject><subject>Regeneration (physiology)</subject><subject>Repair</subject><subject>Signal transduction</subject><subject>Signal Transduction - drug effects</subject><subject>Stem cells</subject><subject>Surgical implants</subject><subject>Testing</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Toxicity</subject><subject>Toxicology</subject><subject>Trauma</subject><subject>Vascular endothelial growth factor</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1uL1DAYhoso7rr6D0QLgujFjGnSpK0Xwrh4GFhY8HQbvqZJm6Ftukmqu_4L_7HpTneZyl5IKT097_ud-kXR0wStE5Ilb3ZmtD2068H0co0wTnDO7kXHSUHwimFE7h_cH0WPnNshREnO2MPoiCQpppSg4-jPexBeWg1tLGTbjq1xMu5kV1roZazGXnhtQhj9W1bxL-2buLmqrLnUMIDXXsbQV-H0elWGPOLO2KExteyl1yIerPFS9zF-G2-mh0473ddxB3NEZWx8LbNykliYYj2OHihonXwyX0-i7x8_fDv9vDo7_7Q93ZytBCuwX9FSlQhnAkEFgLNSViLHIDBBKiMUVJkAI7LIlSKkECRjgJUsGSpQQDPEyEn0fO87hJr53E3HMc5oQVCCs0Bs90RlYMcHqzuwV9yA5tcvjK052FBnKzlN8workYJKixQDLVmVJ4yUFUaYllQFr3dztLHsQq6y9xbahenyS68bXpufnLGiYAUKBq9mA2suRuk8D92cRhbmZMYp75wmKE2yKe8X_6B3VzdTNYQCdK9MiCsmU76hBcsZodnUpfUdVDgq2WkRZqd0eL8QvF4IAuPlpa9hdI5vv375f_b8x5J9ecA2ElrfONOO0y_jlmC6B4U1zlmpbpucID5tzk03-LQ5fN6cIHt2OKBb0c2qkL_o8BeC</recordid><startdate>20190819</startdate><enddate>20190819</enddate><creator>Coelho, Fernanda</creator><creator>Cavicchioli, Maurício</creator><creator>Specian, Sybele Saska</creator><creator>Scarel-Caminaga, Raquel Mantuaneli</creator><creator>Penteado, Letícia de Aquino</creator><creator>Medeiros, Alexandra Ivo de</creator><creator>Ribeiro, Sidney José de Lima</creator><creator>Capote, Ticiana Sidorenko de Oliveira</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5794-4174</orcidid></search><sort><creationdate>20190819</creationdate><title>Bacterial cellulose membrane functionalized with hydroxiapatite and anti-bone morphogenetic protein 2: A promising material for bone regeneration</title><author>Coelho, Fernanda ; Cavicchioli, Maurício ; Specian, Sybele Saska ; Scarel-Caminaga, Raquel Mantuaneli ; Penteado, Letícia de Aquino ; Medeiros, Alexandra Ivo de ; Ribeiro, Sidney José de Lima ; Capote, Ticiana Sidorenko de Oliveira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-5bfb027c0adaa27bedc82ac230f735afb1a63e98ff339c376a2feb6090bed7063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Anti-antibodies</topic><topic>Antibodies</topic><topic>Antibodies, Immobilized - 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drug effects</topic><topic>Cell Line</topic><topic>Cell survival</topic><topic>Cellulose</topic><topic>Cellulose - chemistry</topic><topic>Cellulose - pharmacology</topic><topic>Chemistry</topic><topic>Comet assay</topic><topic>Cytokinesis</topic><topic>Cytotoxicity</topic><topic>Damage detection</topic><topic>Dentistry</topic><topic>Durapatite - chemistry</topic><topic>Durapatite - pharmacology</topic><topic>Endothelial growth factors</topic><topic>Engineering and Technology</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genotoxicity</topic><topic>Gluconacetobacter xylinus - chemistry</topic><topic>Health aspects</topic><topic>Hydroxyapatite</topic><topic>Hydroxyapatites</topic><topic>In vitro methods and tests</topic><topic>In vivo methods and tests</topic><topic>Inorganic chemistry</topic><topic>Kinetics</topic><topic>Materials</topic><topic>Materials Testing</topic><topic>Mechanical properties</topic><topic>Medical research</topic><topic>Medicine and Health Sciences</topic><topic>Membranes</topic><topic>Methods</topic><topic>Mice</topic><topic>Mineralization</topic><topic>Morphology</topic><topic>Mutagenicity</topic><topic>Nanocomposites</topic><topic>Nodules</topic><topic>Organic chemistry</topic><topic>Osteoblasts</topic><topic>Osteogenesis - drug effects</topic><topic>Osteoprotegerin</topic><topic>Peptides</topic><topic>Pharmaceutical sciences</topic><topic>Phosphatases</topic><topic>Physical Sciences</topic><topic>Polymers</topic><topic>Polysaccharides</topic><topic>Proteins</topic><topic>Regeneration</topic><topic>Regeneration (physiology)</topic><topic>Repair</topic><topic>Signal transduction</topic><topic>Signal Transduction - drug effects</topic><topic>Stem cells</topic><topic>Surgical implants</topic><topic>Testing</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Toxicity</topic><topic>Toxicology</topic><topic>Trauma</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coelho, Fernanda</creatorcontrib><creatorcontrib>Cavicchioli, Maurício</creatorcontrib><creatorcontrib>Specian, Sybele Saska</creatorcontrib><creatorcontrib>Scarel-Caminaga, Raquel Mantuaneli</creatorcontrib><creatorcontrib>Penteado, Letícia de Aquino</creatorcontrib><creatorcontrib>Medeiros, Alexandra Ivo de</creatorcontrib><creatorcontrib>Ribeiro, Sidney José de Lima</creatorcontrib><creatorcontrib>Capote, Ticiana Sidorenko de Oliveira</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints in Context (Gale)</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coelho, Fernanda</au><au>Cavicchioli, Maurício</au><au>Specian, Sybele Saska</au><au>Scarel-Caminaga, Raquel Mantuaneli</au><au>Penteado, Letícia de Aquino</au><au>Medeiros, Alexandra Ivo de</au><au>Ribeiro, Sidney José de Lima</au><au>Capote, Ticiana Sidorenko de Oliveira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial cellulose membrane functionalized with hydroxiapatite and anti-bone morphogenetic protein 2: A promising material for bone regeneration</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-08-19</date><risdate>2019</risdate><volume>14</volume><issue>8</issue><spage>e0221286</spage><pages>e0221286-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Bone tissue engineering seeks to adequately restore functions related to physical and biological properties, aiming at a repair process similar to natural bone. The use of compatible biopolymers, such as bacterial cellulose (BC), as well as having interesting mechanical characteristics, presents a slow in vivo degradation rate, and the ability to be chemically modified. To promote better bioactivity towards BC, we synthesized an innovative BC membrane associated to hydroxyapatite (HA) and anti-bone morphogenetic protein antibody (anti-BMP-2) (BC-HA-anti-BMP-2). We present the physical-chemical, biological and toxicological characterization of BC-HA-anti-BMP-2. Presence of BC and HA components in the membranes was confirmed by SEM-EDS and FTIR assays. No toxic potential was found in MC3T3-E1 cells by cytotoxicity assays (XTT Assay and Clonogenic Survival), genotoxicity (Comet Assay) and mutagenicity (Cytokinesis-blocked micronucleus Test). The in vitro release kinetics of anti-BMP-2 antibodies detected gradually reducing antibody levels, reducing approximately 70% in 7 days and 90% in 14 days. BC-HA-anti-BMP-2 increased SPP1, BGLAP, VEGF, ALPL, RUNX2 and TNFRSF11B expression, genes involved in bone repair and also increased mineralization nodules and phosphatase alcalin (ALP) activity levels. In conclusion, we developed BC-HA-anti-BMP-2 as an innovative and promising biomaterial with interesting physical-chemical and biological properties which may be a good alternative to treatment with commercial BMP-2 protein.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31425530</pmid><doi>10.1371/journal.pone.0221286</doi><tpages>e0221286</tpages><orcidid>https://orcid.org/0000-0001-5794-4174</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-08, Vol.14 (8), p.e0221286 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2275930127 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Anti-antibodies Antibodies Antibodies, Immobilized - immunology Antibodies, Immobilized - pharmacology Antibodies, Monoclonal - immunology Antibodies, Monoclonal - pharmacology Bacterial proteins Bioassays Biocompatibility Biodegradation Biological activity Biological products Biological properties Biology and Life Sciences Biomaterials Biomedical materials Biopolymers Bone growth Bone healing Bone morphogenetic protein 2 Bone Morphogenetic Protein 2 - immunology Bone Morphogenetic Protein 2 - metabolism Bone morphogenetic proteins Bone regeneration Bone Regeneration - drug effects Bone Substitutes - chemistry Bone Substitutes - pharmacology Bones Calcium phosphates Cbfa-1 protein Cell adhesion & migration Cell Differentiation - drug effects Cell Line Cell survival Cellulose Cellulose - chemistry Cellulose - pharmacology Chemistry Comet assay Cytokinesis Cytotoxicity Damage detection Dentistry Durapatite - chemistry Durapatite - pharmacology Endothelial growth factors Engineering and Technology Gene expression Genes Genotoxicity Gluconacetobacter xylinus - chemistry Health aspects Hydroxyapatite Hydroxyapatites In vitro methods and tests In vivo methods and tests Inorganic chemistry Kinetics Materials Materials Testing Mechanical properties Medical research Medicine and Health Sciences Membranes Methods Mice Mineralization Morphology Mutagenicity Nanocomposites Nodules Organic chemistry Osteoblasts Osteogenesis - drug effects Osteoprotegerin Peptides Pharmaceutical sciences Phosphatases Physical Sciences Polymers Polysaccharides Proteins Regeneration Regeneration (physiology) Repair Signal transduction Signal Transduction - drug effects Stem cells Surgical implants Testing Tissue engineering Tissue Engineering - methods Toxicity Toxicology Trauma Vascular endothelial growth factor |
| title | Bacterial cellulose membrane functionalized with hydroxiapatite and anti-bone morphogenetic protein 2: A promising material for bone regeneration |
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