Rapid and highly sensitive detection of pyocyanin biomarker in different Pseudomonas aeruginosa infections using gold nanoparticles modified sensor
Successful antibiotic treatment of infections relies on accurate and rapid identification of the infectious agents. Pseudomonas aeruginosa is implicated in a wide range of human infections that mostly become complicated and life threating, especially in immunocompromised and critically ill patients....
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| Veröffentlicht in: | PloS one 2019-07, Vol.14 (7), p.e0216438-e0216438 |
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| creator | Elkhawaga, Amal A Khalifa, Marwa M El-Badawy, Omnia Hassan, Mona A El-Said, Waleed A |
| description | Successful antibiotic treatment of infections relies on accurate and rapid identification of the infectious agents. Pseudomonas aeruginosa is implicated in a wide range of human infections that mostly become complicated and life threating, especially in immunocompromised and critically ill patients. Conventional microbiological methods take more than three days to obtain accurate results. Pyocyanin is a distinctive electroactive biomarker for Pseudomonas aeruginosa. Here, we have prepared polyaniline/gold nanoparticles decorated ITO electrode and tested it to establish a rapid, diagnostic and highly sensitive pyocyanin sensor in a culture of Pseudomonas aeruginosa clinical isolates with high selectivity for traces of pyocyanin when measured in the existence of different interferences like vitamin C, uric acid, and glucose. The scanning electron microscopy and cyclic voltammetry techniques were used to characterize the morphology and electrical conductivity of the constructed electrode. The determined linear range for pyocyanin detection was from 238 μM to 1.9 μM with a detection limit of 500 nM. Compared to the screen-printed electrode used before, the constructed electrode showed a 4-fold enhanced performance. Furthermore, PANI/Au NPs/ITO modified electrodes have demonstrated the ability to detect pyocyanin directly in Pseudomonas aeruginosa culture without any potential interference with other species. |
| doi_str_mv | 10.1371/journal.pone.0216438 |
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Pseudomonas aeruginosa is implicated in a wide range of human infections that mostly become complicated and life threating, especially in immunocompromised and critically ill patients. Conventional microbiological methods take more than three days to obtain accurate results. Pyocyanin is a distinctive electroactive biomarker for Pseudomonas aeruginosa. Here, we have prepared polyaniline/gold nanoparticles decorated ITO electrode and tested it to establish a rapid, diagnostic and highly sensitive pyocyanin sensor in a culture of Pseudomonas aeruginosa clinical isolates with high selectivity for traces of pyocyanin when measured in the existence of different interferences like vitamin C, uric acid, and glucose. The scanning electron microscopy and cyclic voltammetry techniques were used to characterize the morphology and electrical conductivity of the constructed electrode. The determined linear range for pyocyanin detection was from 238 μM to 1.9 μM with a detection limit of 500 nM. Compared to the screen-printed electrode used before, the constructed electrode showed a 4-fold enhanced performance. Furthermore, PANI/Au NPs/ITO modified electrodes have demonstrated the ability to detect pyocyanin directly in Pseudomonas aeruginosa culture without any potential interference with other species.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0216438</identifier><identifier>PMID: 31361746</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analytical chemistry ; Aniline Compounds - chemistry ; Antibiotics ; Ascorbic acid ; Bacteria ; Bioindicators ; Biological markers ; Biology and Life Sciences ; Biomarkers ; Biomarkers - analysis ; Biosensing Techniques - economics ; Biosensing Techniques - methods ; Biosensors ; Carbon ; Clinical isolates ; Conductivity ; Critically ill persons ; Culture ; Diagnostic systems ; Electrical conductivity ; Electrical resistivity ; Electrochemical Techniques - economics ; Electrochemical Techniques - methods ; Electrodes ; Electron microscopy ; Engineering and Technology ; Genetic research ; Glucose ; Gold ; Gold - chemistry ; Health aspects ; Humans ; Immunology ; Infection ; Infections ; Limit of Detection ; Medicine ; Medicine and Health Sciences ; Metal Nanoparticles - chemistry ; Microbiology ; Microscopy ; Morphology ; Nanocomposites ; Nanoparticles ; Organic light emitting diodes ; Pathogens ; Performance enhancement ; Physical Sciences ; Polyanilines ; Polymers ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - isolation & purification ; Pseudomonas aeruginosa infections ; Pseudomonas Infections - diagnosis ; Pseudomonas Infections - microbiology ; Purines ; Pyocyanin ; Pyocyanine - analysis ; Research and Analysis Methods ; Scanning electron microscopy ; Selectivity ; Sensors ; Time Factors ; Uric acid ; Vitamin C ; Voltammetry</subject><ispartof>PloS one, 2019-07, Vol.14 (7), p.e0216438-e0216438</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Elkhawaga et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Pseudomonas aeruginosa is implicated in a wide range of human infections that mostly become complicated and life threating, especially in immunocompromised and critically ill patients. Conventional microbiological methods take more than three days to obtain accurate results. Pyocyanin is a distinctive electroactive biomarker for Pseudomonas aeruginosa. Here, we have prepared polyaniline/gold nanoparticles decorated ITO electrode and tested it to establish a rapid, diagnostic and highly sensitive pyocyanin sensor in a culture of Pseudomonas aeruginosa clinical isolates with high selectivity for traces of pyocyanin when measured in the existence of different interferences like vitamin C, uric acid, and glucose. The scanning electron microscopy and cyclic voltammetry techniques were used to characterize the morphology and electrical conductivity of the constructed electrode. The determined linear range for pyocyanin detection was from 238 μM to 1.9 μM with a detection limit of 500 nM. Compared to the screen-printed electrode used before, the constructed electrode showed a 4-fold enhanced performance. Furthermore, PANI/Au NPs/ITO modified electrodes have demonstrated the ability to detect pyocyanin directly in Pseudomonas aeruginosa culture without any potential interference with other species.</description><subject>Analytical chemistry</subject><subject>Aniline Compounds - chemistry</subject><subject>Antibiotics</subject><subject>Ascorbic acid</subject><subject>Bacteria</subject><subject>Bioindicators</subject><subject>Biological markers</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biomarkers - analysis</subject><subject>Biosensing Techniques - economics</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Carbon</subject><subject>Clinical isolates</subject><subject>Conductivity</subject><subject>Critically ill persons</subject><subject>Culture</subject><subject>Diagnostic systems</subject><subject>Electrical conductivity</subject><subject>Electrical resistivity</subject><subject>Electrochemical Techniques - 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Pseudomonas aeruginosa is implicated in a wide range of human infections that mostly become complicated and life threating, especially in immunocompromised and critically ill patients. Conventional microbiological methods take more than three days to obtain accurate results. Pyocyanin is a distinctive electroactive biomarker for Pseudomonas aeruginosa. Here, we have prepared polyaniline/gold nanoparticles decorated ITO electrode and tested it to establish a rapid, diagnostic and highly sensitive pyocyanin sensor in a culture of Pseudomonas aeruginosa clinical isolates with high selectivity for traces of pyocyanin when measured in the existence of different interferences like vitamin C, uric acid, and glucose. The scanning electron microscopy and cyclic voltammetry techniques were used to characterize the morphology and electrical conductivity of the constructed electrode. The determined linear range for pyocyanin detection was from 238 μM to 1.9 μM with a detection limit of 500 nM. Compared to the screen-printed electrode used before, the constructed electrode showed a 4-fold enhanced performance. Furthermore, PANI/Au NPs/ITO modified electrodes have demonstrated the ability to detect pyocyanin directly in Pseudomonas aeruginosa culture without any potential interference with other species.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31361746</pmid><doi>10.1371/journal.pone.0216438</doi><tpages>e0216438</tpages><orcidid>https://orcid.org/0000-0002-7052-7612</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2019-07, Vol.14 (7), p.e0216438-e0216438 |
| issn | 1932-6203 1932-6203 |
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| recordid | cdi_plos_journals_2267005721 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Analytical chemistry Aniline Compounds - chemistry Antibiotics Ascorbic acid Bacteria Bioindicators Biological markers Biology and Life Sciences Biomarkers Biomarkers - analysis Biosensing Techniques - economics Biosensing Techniques - methods Biosensors Carbon Clinical isolates Conductivity Critically ill persons Culture Diagnostic systems Electrical conductivity Electrical resistivity Electrochemical Techniques - economics Electrochemical Techniques - methods Electrodes Electron microscopy Engineering and Technology Genetic research Glucose Gold Gold - chemistry Health aspects Humans Immunology Infection Infections Limit of Detection Medicine Medicine and Health Sciences Metal Nanoparticles - chemistry Microbiology Microscopy Morphology Nanocomposites Nanoparticles Organic light emitting diodes Pathogens Performance enhancement Physical Sciences Polyanilines Polymers Pseudomonas aeruginosa Pseudomonas aeruginosa - isolation & purification Pseudomonas aeruginosa infections Pseudomonas Infections - diagnosis Pseudomonas Infections - microbiology Purines Pyocyanin Pyocyanine - analysis Research and Analysis Methods Scanning electron microscopy Selectivity Sensors Time Factors Uric acid Vitamin C Voltammetry |
| title | Rapid and highly sensitive detection of pyocyanin biomarker in different Pseudomonas aeruginosa infections using gold nanoparticles modified sensor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T16%3A21%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20and%20highly%20sensitive%20detection%20of%20pyocyanin%20biomarker%20in%20different%20Pseudomonas%20aeruginosa%20infections%20using%20gold%20nanoparticles%20modified%20sensor&rft.jtitle=PloS%20one&rft.au=Elkhawaga,%20Amal%20A&rft.date=2019-07-30&rft.volume=14&rft.issue=7&rft.spage=e0216438&rft.epage=e0216438&rft.pages=e0216438-e0216438&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0216438&rft_dat=%3Cgale_plos_%3EA594981728%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2267005721&rft_id=info:pmid/31361746&rft_galeid=A594981728&rft_doaj_id=oai_doaj_org_article_ba606cb5e5f74b3e971c643901dfd8d2&rfr_iscdi=true |