Resistance of Neisseria gonorrhoeae isolates to beta-lactam antibiotics (benzylpenicillin and ceftriaxone) in Russia, 2015-2017
The goal of this work was to study the phenotypic susceptibility and resistance determinants of N. gonorrhoeae isolates to beta-lactam antimicrobials (benzylpenicillin and ceftriaxone). A total of 522 clinical isolates collected in Russia in 2015-2017 were analysed for susceptibility using the agar...
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| Veröffentlicht in: | PloS one 2019-07, Vol.14 (7), p.e0220339-e0220339 |
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| creator | Shaskolskiy, Boris Dementieva, Ekaterina Kandinov, Ilya Filippova, Marina Petrova, Natalia Plakhova, Xenia Chestkov, Alexander Kubanov, Alexey Deryabin, Dmitry Gryadunov, Dmitry |
| description | The goal of this work was to study the phenotypic susceptibility and resistance determinants of N. gonorrhoeae isolates to beta-lactam antimicrobials (benzylpenicillin and ceftriaxone). A total of 522 clinical isolates collected in Russia in 2015-2017 were analysed for susceptibility using the agar dilution method. DNA loci involved in antimicrobial resistance were identified using DNA microarray analysis and sequencing. Resistance to benzylpenicillin remained high, with 7.7% of isolates resistant (MICpen > 1 mg/L) and 47.5% of isolates showing intermediate susceptibility (MICpen = 0.12-1 mg/L). The most frequent resistance determinant (72.4% isolates) was the Asp345 insertion in penA, both as a single mutation and in combination with other mutations, particularly with the substitution Leu421Pro in ponA (39.0%). Mutations affecting the influx and efflux of drugs were also found, including amino acid substitutions in PorB (26.8% isolates) and delA in the promoter region of mtrR (22.8%). The accumulation of mutations in chromosomal genes (penA, pon, porA, and mtrR) led to a stepwise increase in MICpen to values characteristic of intermediate resistance. The presence of blaTEM plasmids was found in 25 isolates (4.8%), resulting in a strong increase in resistance to penicillin (MICpen > 16 mg/L) compared with the chromosomal mutations; 23 plasmids were of the African type with TEM-1 beta-lactamase, and two plasmids were of the Toronto/Rio type with TEM-135 beta-lactamase. Only three isolates were found with reduced susceptibility to ceftriaxone, with MICcef = 0.12-0.25 mg/L. Sequencing of penA did not reveal mutations associated with resistance to third-generation cephalosporins, and the gene structure was non-mosaic. The majority of isolates (21 of 25) carrying the blaTEM plasmid also contained the conjugative plasmid with tetM (resistance to tetracyclines), consistent with previously reported data that the presence of the conjugative plasmid facilitates the transfer of other plasmids associated with antimicrobial resistance. |
| doi_str_mv | 10.1371/journal.pone.0220339 |
| format | Article |
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A total of 522 clinical isolates collected in Russia in 2015-2017 were analysed for susceptibility using the agar dilution method. DNA loci involved in antimicrobial resistance were identified using DNA microarray analysis and sequencing. Resistance to benzylpenicillin remained high, with 7.7% of isolates resistant (MICpen > 1 mg/L) and 47.5% of isolates showing intermediate susceptibility (MICpen = 0.12-1 mg/L). The most frequent resistance determinant (72.4% isolates) was the Asp345 insertion in penA, both as a single mutation and in combination with other mutations, particularly with the substitution Leu421Pro in ponA (39.0%). Mutations affecting the influx and efflux of drugs were also found, including amino acid substitutions in PorB (26.8% isolates) and delA in the promoter region of mtrR (22.8%). The accumulation of mutations in chromosomal genes (penA, pon, porA, and mtrR) led to a stepwise increase in MICpen to values characteristic of intermediate resistance. The presence of blaTEM plasmids was found in 25 isolates (4.8%), resulting in a strong increase in resistance to penicillin (MICpen > 16 mg/L) compared with the chromosomal mutations; 23 plasmids were of the African type with TEM-1 beta-lactamase, and two plasmids were of the Toronto/Rio type with TEM-135 beta-lactamase. Only three isolates were found with reduced susceptibility to ceftriaxone, with MICcef = 0.12-0.25 mg/L. Sequencing of penA did not reveal mutations associated with resistance to third-generation cephalosporins, and the gene structure was non-mosaic. The majority of isolates (21 of 25) carrying the blaTEM plasmid also contained the conjugative plasmid with tetM (resistance to tetracyclines), consistent with previously reported data that the presence of the conjugative plasmid facilitates the transfer of other plasmids associated with antimicrobial resistance.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0220339</identifier><identifier>PMID: 31344102</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Amides ; Amino Acid Substitution - genetics ; Amino acids ; Anti-Bacterial Agents - pharmacology ; Anti-Bacterial Agents - therapeutic use ; Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial agents ; Antimicrobial resistance ; Benzylpenicillin ; Beta lactam antibiotics ; Beta lactamases ; beta-Lactam Resistance - drug effects ; beta-Lactam Resistance - genetics ; beta-Lactamases - genetics ; Biology and Life Sciences ; Ceftriaxone ; Ceftriaxone - pharmacology ; Ceftriaxone - therapeutic use ; Cephalosporins ; Clinical isolates ; Cosmetology ; Deoxyribonucleic acid ; Dilution ; Distribution ; DNA ; DNA chips ; DNA microarrays ; DNA Mutational Analysis ; DNA sequencing ; Dosage and administration ; Drug resistance ; Drug Resistance, Bacterial - drug effects ; Drug Resistance, Bacterial - genetics ; Drug therapy ; Efflux ; Female ; Gene mutation ; Gene sequencing ; Genes ; Gonorrhea ; Gonorrhea - drug therapy ; Gonorrhea - epidemiology ; Gonorrhea - microbiology ; Humans ; Laboratories ; Lactams ; Male ; Medicine and Health Sciences ; Microbial drug resistance ; Microbial Sensitivity Tests ; Mitochondrial DNA ; Molecular biology ; Mutation ; Neisseria gonorrhoeae - drug effects ; Neisseria gonorrhoeae - genetics ; Neisseria gonorrhoeae - isolation & purification ; Neisseria infections ; Penicillin ; Penicillin G ; Penicillin G - pharmacology ; Penicillin G - therapeutic use ; Penicillins ; People and Places ; Plasmids ; Polymorphism, Genetic ; Prevention ; Proteins ; Russia - epidemiology ; Surveillance ; Tetracyclines ; β Lactamase ; β-Lactam antibiotics</subject><ispartof>PloS one, 2019-07, Vol.14 (7), p.e0220339-e0220339</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Shaskolskiy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Shaskolskiy et al 2019 Shaskolskiy et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c659t-cff98ad175c4176cef26499599ae79997a85cd7579fc0054d20af4b9aada59613</citedby><cites>FETCH-LOGICAL-c659t-cff98ad175c4176cef26499599ae79997a85cd7579fc0054d20af4b9aada59613</cites><orcidid>0000-0002-0316-2262</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657886/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657886/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2919,23857,27915,27916,53782,53784,79361,79362</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31344102$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Shafer, William M.</contributor><creatorcontrib>Shaskolskiy, Boris</creatorcontrib><creatorcontrib>Dementieva, Ekaterina</creatorcontrib><creatorcontrib>Kandinov, Ilya</creatorcontrib><creatorcontrib>Filippova, Marina</creatorcontrib><creatorcontrib>Petrova, Natalia</creatorcontrib><creatorcontrib>Plakhova, Xenia</creatorcontrib><creatorcontrib>Chestkov, Alexander</creatorcontrib><creatorcontrib>Kubanov, Alexey</creatorcontrib><creatorcontrib>Deryabin, Dmitry</creatorcontrib><creatorcontrib>Gryadunov, Dmitry</creatorcontrib><title>Resistance of Neisseria gonorrhoeae isolates to beta-lactam antibiotics (benzylpenicillin and ceftriaxone) in Russia, 2015-2017</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The goal of this work was to study the phenotypic susceptibility and resistance determinants of N. gonorrhoeae isolates to beta-lactam antimicrobials (benzylpenicillin and ceftriaxone). A total of 522 clinical isolates collected in Russia in 2015-2017 were analysed for susceptibility using the agar dilution method. DNA loci involved in antimicrobial resistance were identified using DNA microarray analysis and sequencing. Resistance to benzylpenicillin remained high, with 7.7% of isolates resistant (MICpen > 1 mg/L) and 47.5% of isolates showing intermediate susceptibility (MICpen = 0.12-1 mg/L). The most frequent resistance determinant (72.4% isolates) was the Asp345 insertion in penA, both as a single mutation and in combination with other mutations, particularly with the substitution Leu421Pro in ponA (39.0%). Mutations affecting the influx and efflux of drugs were also found, including amino acid substitutions in PorB (26.8% isolates) and delA in the promoter region of mtrR (22.8%). The accumulation of mutations in chromosomal genes (penA, pon, porA, and mtrR) led to a stepwise increase in MICpen to values characteristic of intermediate resistance. The presence of blaTEM plasmids was found in 25 isolates (4.8%), resulting in a strong increase in resistance to penicillin (MICpen > 16 mg/L) compared with the chromosomal mutations; 23 plasmids were of the African type with TEM-1 beta-lactamase, and two plasmids were of the Toronto/Rio type with TEM-135 beta-lactamase. Only three isolates were found with reduced susceptibility to ceftriaxone, with MICcef = 0.12-0.25 mg/L. Sequencing of penA did not reveal mutations associated with resistance to third-generation cephalosporins, and the gene structure was non-mosaic. The majority of isolates (21 of 25) carrying the blaTEM plasmid also contained the conjugative plasmid with tetM (resistance to tetracyclines), consistent with previously reported data that the presence of the conjugative plasmid facilitates the transfer of other plasmids associated with antimicrobial resistance.</description><subject>Adult</subject><subject>Amides</subject><subject>Amino Acid Substitution - genetics</subject><subject>Amino acids</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial resistance</subject><subject>Benzylpenicillin</subject><subject>Beta lactam antibiotics</subject><subject>Beta lactamases</subject><subject>beta-Lactam Resistance - drug effects</subject><subject>beta-Lactam Resistance - genetics</subject><subject>beta-Lactamases - genetics</subject><subject>Biology and Life Sciences</subject><subject>Ceftriaxone</subject><subject>Ceftriaxone - pharmacology</subject><subject>Ceftriaxone - therapeutic use</subject><subject>Cephalosporins</subject><subject>Clinical isolates</subject><subject>Cosmetology</subject><subject>Deoxyribonucleic acid</subject><subject>Dilution</subject><subject>Distribution</subject><subject>DNA</subject><subject>DNA chips</subject><subject>DNA microarrays</subject><subject>DNA Mutational Analysis</subject><subject>DNA sequencing</subject><subject>Dosage and administration</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial - drug effects</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Drug therapy</subject><subject>Efflux</subject><subject>Female</subject><subject>Gene mutation</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Gonorrhea</subject><subject>Gonorrhea - drug therapy</subject><subject>Gonorrhea - epidemiology</subject><subject>Gonorrhea - microbiology</subject><subject>Humans</subject><subject>Laboratories</subject><subject>Lactams</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Microbial drug resistance</subject><subject>Microbial Sensitivity Tests</subject><subject>Mitochondrial DNA</subject><subject>Molecular biology</subject><subject>Mutation</subject><subject>Neisseria gonorrhoeae - drug effects</subject><subject>Neisseria gonorrhoeae - genetics</subject><subject>Neisseria gonorrhoeae - isolation & purification</subject><subject>Neisseria infections</subject><subject>Penicillin</subject><subject>Penicillin G</subject><subject>Penicillin G - pharmacology</subject><subject>Penicillin G - therapeutic use</subject><subject>Penicillins</subject><subject>People and Places</subject><subject>Plasmids</subject><subject>Polymorphism, Genetic</subject><subject>Prevention</subject><subject>Proteins</subject><subject>Russia - epidemiology</subject><subject>Surveillance</subject><subject>Tetracyclines</subject><subject>β Lactamase</subject><subject>β-Lactam antibiotics</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUm1rFDEQXkSxtfoPRBf8UsE9N5u3zRehFF8KRaHo5zCbzF5TcsmZ7In1i3_dXG9belICeZl55pl5JlNVL0m7IFSS91dxkwL4xToGXLRd11KqHlWHRNGuEeX1-N79oHqW81XbctoL8bQ6oIQyRtrusPp7gdnlCYLBOo71V3Q5Y3JQL2OIKV1GBKxdjh4mzPUU6wEnaDyYCVY1hMkNLk7O5Pp4wPDn2q8xOOO8d6F4bW1wnArb71Lj27rYLjY5O3hXdy3hTdnk8-rJCD7ji_k8qn58-vj99Etz_u3z2enJeWMEV1NjxlH1YInkhhEpCm0nmFJcKUCplJLQc2Mll2o0RSazXQsjGxSABa4EoUfV6x3v2ses595l3RUaRqUSoiDOdggb4Uqvk1tButYRnL4xxLTUkIpUj9pKglbAYJmhjPNOdYPtCGGSDmzsURauD3O2zbBCazBMCfwe6b4nuEu9jL-0EFz2_baY45kgxZ8bzJNeuWzQewgYNzd1cymKLl6gb_6DPqxuRi2hCHBhjCWv2ZLqE64Yb9WuS4sHUGVZXDlTPnF0xb4XwHYBJsWcE453Gkmrt2N6W4zejqmex7SEvbrfn7ug27mk_wB_QeT-</recordid><startdate>20190725</startdate><enddate>20190725</enddate><creator>Shaskolskiy, Boris</creator><creator>Dementieva, Ekaterina</creator><creator>Kandinov, Ilya</creator><creator>Filippova, Marina</creator><creator>Petrova, Natalia</creator><creator>Plakhova, Xenia</creator><creator>Chestkov, Alexander</creator><creator>Kubanov, Alexey</creator><creator>Deryabin, Dmitry</creator><creator>Gryadunov, Dmitry</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-0316-2262</orcidid></search><sort><creationdate>20190725</creationdate><title>Resistance of Neisseria gonorrhoeae isolates to beta-lactam antibiotics (benzylpenicillin and ceftriaxone) in Russia, 2015-2017</title><author>Shaskolskiy, Boris ; Dementieva, Ekaterina ; Kandinov, Ilya ; Filippova, Marina ; Petrova, Natalia ; Plakhova, Xenia ; Chestkov, Alexander ; Kubanov, Alexey ; Deryabin, Dmitry ; Gryadunov, Dmitry</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c659t-cff98ad175c4176cef26499599ae79997a85cd7579fc0054d20af4b9aada59613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Amides</topic><topic>Amino Acid Substitution - genetics</topic><topic>Amino acids</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Anti-Bacterial Agents - therapeutic use</topic><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial agents</topic><topic>Antimicrobial resistance</topic><topic>Benzylpenicillin</topic><topic>Beta lactam antibiotics</topic><topic>Beta lactamases</topic><topic>beta-Lactam Resistance - drug effects</topic><topic>beta-Lactam Resistance - genetics</topic><topic>beta-Lactamases - genetics</topic><topic>Biology and Life Sciences</topic><topic>Ceftriaxone</topic><topic>Ceftriaxone - pharmacology</topic><topic>Ceftriaxone - therapeutic use</topic><topic>Cephalosporins</topic><topic>Clinical isolates</topic><topic>Cosmetology</topic><topic>Deoxyribonucleic acid</topic><topic>Dilution</topic><topic>Distribution</topic><topic>DNA</topic><topic>DNA chips</topic><topic>DNA microarrays</topic><topic>DNA Mutational Analysis</topic><topic>DNA sequencing</topic><topic>Dosage and administration</topic><topic>Drug resistance</topic><topic>Drug Resistance, Bacterial - drug effects</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Drug therapy</topic><topic>Efflux</topic><topic>Female</topic><topic>Gene mutation</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Gonorrhea</topic><topic>Gonorrhea - drug therapy</topic><topic>Gonorrhea - epidemiology</topic><topic>Gonorrhea - microbiology</topic><topic>Humans</topic><topic>Laboratories</topic><topic>Lactams</topic><topic>Male</topic><topic>Medicine and Health Sciences</topic><topic>Microbial drug resistance</topic><topic>Microbial Sensitivity Tests</topic><topic>Mitochondrial DNA</topic><topic>Molecular biology</topic><topic>Mutation</topic><topic>Neisseria gonorrhoeae - drug effects</topic><topic>Neisseria gonorrhoeae - genetics</topic><topic>Neisseria gonorrhoeae - isolation & purification</topic><topic>Neisseria infections</topic><topic>Penicillin</topic><topic>Penicillin G</topic><topic>Penicillin G - pharmacology</topic><topic>Penicillin G - therapeutic use</topic><topic>Penicillins</topic><topic>People and Places</topic><topic>Plasmids</topic><topic>Polymorphism, Genetic</topic><topic>Prevention</topic><topic>Proteins</topic><topic>Russia - epidemiology</topic><topic>Surveillance</topic><topic>Tetracyclines</topic><topic>β Lactamase</topic><topic>β-Lactam antibiotics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shaskolskiy, Boris</creatorcontrib><creatorcontrib>Dementieva, Ekaterina</creatorcontrib><creatorcontrib>Kandinov, Ilya</creatorcontrib><creatorcontrib>Filippova, Marina</creatorcontrib><creatorcontrib>Petrova, Natalia</creatorcontrib><creatorcontrib>Plakhova, Xenia</creatorcontrib><creatorcontrib>Chestkov, Alexander</creatorcontrib><creatorcontrib>Kubanov, Alexey</creatorcontrib><creatorcontrib>Deryabin, Dmitry</creatorcontrib><creatorcontrib>Gryadunov, Dmitry</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Database (1962 - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shaskolskiy, Boris</au><au>Dementieva, Ekaterina</au><au>Kandinov, Ilya</au><au>Filippova, Marina</au><au>Petrova, Natalia</au><au>Plakhova, Xenia</au><au>Chestkov, Alexander</au><au>Kubanov, Alexey</au><au>Deryabin, Dmitry</au><au>Gryadunov, Dmitry</au><au>Shafer, William M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resistance of Neisseria gonorrhoeae isolates to beta-lactam antibiotics (benzylpenicillin and ceftriaxone) in Russia, 2015-2017</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-07-25</date><risdate>2019</risdate><volume>14</volume><issue>7</issue><spage>e0220339</spage><epage>e0220339</epage><pages>e0220339-e0220339</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The goal of this work was to study the phenotypic susceptibility and resistance determinants of N. gonorrhoeae isolates to beta-lactam antimicrobials (benzylpenicillin and ceftriaxone). A total of 522 clinical isolates collected in Russia in 2015-2017 were analysed for susceptibility using the agar dilution method. DNA loci involved in antimicrobial resistance were identified using DNA microarray analysis and sequencing. Resistance to benzylpenicillin remained high, with 7.7% of isolates resistant (MICpen > 1 mg/L) and 47.5% of isolates showing intermediate susceptibility (MICpen = 0.12-1 mg/L). The most frequent resistance determinant (72.4% isolates) was the Asp345 insertion in penA, both as a single mutation and in combination with other mutations, particularly with the substitution Leu421Pro in ponA (39.0%). Mutations affecting the influx and efflux of drugs were also found, including amino acid substitutions in PorB (26.8% isolates) and delA in the promoter region of mtrR (22.8%). The accumulation of mutations in chromosomal genes (penA, pon, porA, and mtrR) led to a stepwise increase in MICpen to values characteristic of intermediate resistance. The presence of blaTEM plasmids was found in 25 isolates (4.8%), resulting in a strong increase in resistance to penicillin (MICpen > 16 mg/L) compared with the chromosomal mutations; 23 plasmids were of the African type with TEM-1 beta-lactamase, and two plasmids were of the Toronto/Rio type with TEM-135 beta-lactamase. Only three isolates were found with reduced susceptibility to ceftriaxone, with MICcef = 0.12-0.25 mg/L. Sequencing of penA did not reveal mutations associated with resistance to third-generation cephalosporins, and the gene structure was non-mosaic. The majority of isolates (21 of 25) carrying the blaTEM plasmid also contained the conjugative plasmid with tetM (resistance to tetracyclines), consistent with previously reported data that the presence of the conjugative plasmid facilitates the transfer of other plasmids associated with antimicrobial resistance.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31344102</pmid><doi>10.1371/journal.pone.0220339</doi><orcidid>https://orcid.org/0000-0002-0316-2262</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-07, Vol.14 (7), p.e0220339-e0220339 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2264437966 |
| source | PLoS; MEDLINE; Full-Text Journals in Chemistry (Open access); DOAJ Directory of Open Access Journals; PubMed Central; EZB Electronic Journals Library |
| subjects | Adult Amides Amino Acid Substitution - genetics Amino acids Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Antibiotics Antiinfectives and antibacterials Antimicrobial agents Antimicrobial resistance Benzylpenicillin Beta lactam antibiotics Beta lactamases beta-Lactam Resistance - drug effects beta-Lactam Resistance - genetics beta-Lactamases - genetics Biology and Life Sciences Ceftriaxone Ceftriaxone - pharmacology Ceftriaxone - therapeutic use Cephalosporins Clinical isolates Cosmetology Deoxyribonucleic acid Dilution Distribution DNA DNA chips DNA microarrays DNA Mutational Analysis DNA sequencing Dosage and administration Drug resistance Drug Resistance, Bacterial - drug effects Drug Resistance, Bacterial - genetics Drug therapy Efflux Female Gene mutation Gene sequencing Genes Gonorrhea Gonorrhea - drug therapy Gonorrhea - epidemiology Gonorrhea - microbiology Humans Laboratories Lactams Male Medicine and Health Sciences Microbial drug resistance Microbial Sensitivity Tests Mitochondrial DNA Molecular biology Mutation Neisseria gonorrhoeae - drug effects Neisseria gonorrhoeae - genetics Neisseria gonorrhoeae - isolation & purification Neisseria infections Penicillin Penicillin G Penicillin G - pharmacology Penicillin G - therapeutic use Penicillins People and Places Plasmids Polymorphism, Genetic Prevention Proteins Russia - epidemiology Surveillance Tetracyclines β Lactamase β-Lactam antibiotics |
| title | Resistance of Neisseria gonorrhoeae isolates to beta-lactam antibiotics (benzylpenicillin and ceftriaxone) in Russia, 2015-2017 |
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