Rapid and sensitive detection of Chlamydia trachomatis sexually transmitted infections in resource-constrained settings in Thailand at the point-of-care
Infection is often asymptomatic, causing the epidemiology to be underestimated. [...]we have developed a rapid, inexpensive, easy-to-interpret, sensitive and specific point-of-care (POC) C. trachomatis detection system, using loop-mediated isothermal amplification (LAMP) for target C. trachomatis DN...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2018-12, Vol.12 (12), p.e0006900-e0006900 |
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| creator | Somboonna, Naraporn Choopara, Ilada Arunrut, Narong Sukhonpan, Kanchapan Sayasathid, Jarun Dean, Deborah Kiatpathomchai, Wansika |
| description | Infection is often asymptomatic, causing the epidemiology to be underestimated. [...]we have developed a rapid, inexpensive, easy-to-interpret, sensitive and specific point-of-care (POC) C. trachomatis detection system, using loop-mediated isothermal amplification (LAMP) for target C. trachomatis DNA amplification, followed by gold nanoparticle probe (AuNP) for colorimetric C. trachomatis specific readout. Results Optimization of LAMP-AuNP Loop primers LF and LB, which specifically locate the dumbbell products of LAMP, allow for extra amplification of the loop amplicons. [...]a sufficient amount of amplicons for detection by GE (or AuNP) were made at the shorter assay time. The results demonstrated an LOD of 22.5 copies for PCR-GE and 45 copies for LAMP-GE and LAMP-AuNP. [...]increasing the LAMP reaction incubation from 20 to 35 minutes allowed the LOD to become consistent at 11.25 copies (S4 Fig, lane 1). The colorimetric change of the LAMP-AuNP was confirmed by UV-vis spectrophotometry [7]. [...]the LAMP-AuNP has an LOD of 45 copies when incubated for 20 minutes and 11.25 copies when incubated for 35 minutes. |
| doi_str_mv | 10.1371/journal.pntd.0006900 |
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C.</contributor><creatorcontrib>Somboonna, Naraporn ; Choopara, Ilada ; Arunrut, Narong ; Sukhonpan, Kanchapan ; Sayasathid, Jarun ; Dean, Deborah ; Kiatpathomchai, Wansika ; Small, Pamela L. C.</creatorcontrib><description>Infection is often asymptomatic, causing the epidemiology to be underestimated. [...]we have developed a rapid, inexpensive, easy-to-interpret, sensitive and specific point-of-care (POC) C. trachomatis detection system, using loop-mediated isothermal amplification (LAMP) for target C. trachomatis DNA amplification, followed by gold nanoparticle probe (AuNP) for colorimetric C. trachomatis specific readout. Results Optimization of LAMP-AuNP Loop primers LF and LB, which specifically locate the dumbbell products of LAMP, allow for extra amplification of the loop amplicons. [...]a sufficient amount of amplicons for detection by GE (or AuNP) were made at the shorter assay time. The results demonstrated an LOD of 22.5 copies for PCR-GE and 45 copies for LAMP-GE and LAMP-AuNP. [...]increasing the LAMP reaction incubation from 20 to 35 minutes allowed the LOD to become consistent at 11.25 copies (S4 Fig, lane 1). The colorimetric change of the LAMP-AuNP was confirmed by UV-vis spectrophotometry [7]. [...]the LAMP-AuNP has an LOD of 45 copies when incubated for 20 minutes and 11.25 copies when incubated for 35 minutes.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0006900</identifier><identifier>PMID: 30571705</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Bacteria ; Bioengineering ; Biology and Life Sciences ; Biotechnology ; Causes of ; Chlamydia ; Chlamydia Infections - diagnosis ; Chlamydia Infections - microbiology ; Chlamydia Infections - prevention & control ; Chlamydia trachomatis ; Chlamydia trachomatis - genetics ; Chlamydia trachomatis - isolation & purification ; Colorimetry ; Condoms ; Deoxyribonucleic acid ; Detection ; Diagnosis ; Disease control ; Disease prevention ; Distribution ; DNA ; DNA polymerase ; Epidemiology ; Female ; From Innovation to Application ; Genetic engineering ; Gold ; Hospitals ; Humans ; Incubation period ; Infections ; Male ; Medical laboratories ; Medicine and Health Sciences ; Nanoparticles ; Nucleotide sequence ; Optimization ; PCR ; Point-of-Care Systems ; Polymerase chain reaction ; Primers ; Research and Analysis Methods ; Sensitivity and Specificity ; Sexually transmitted diseases ; Spectrophotometry ; STD ; Thailand ; Time Factors ; Tropical diseases ; Womens health</subject><ispartof>PLoS neglected tropical diseases, 2018-12, Vol.12 (12), p.e0006900-e0006900</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication: https://creativecommons.org/publicdomain/zero/1.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c624t-d2ff21240e40489982c40f6ee08ed6f8b964d486c5c3dfce5c5169b10b466eef3</citedby><cites>FETCH-LOGICAL-c624t-d2ff21240e40489982c40f6ee08ed6f8b964d486c5c3dfce5c5169b10b466eef3</cites><orcidid>0000-0002-7830-3509</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6301561/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6301561/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2919,23857,27915,27916,53782,53784,79361,79362</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30571705$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Small, Pamela L. C.</contributor><creatorcontrib>Somboonna, Naraporn</creatorcontrib><creatorcontrib>Choopara, Ilada</creatorcontrib><creatorcontrib>Arunrut, Narong</creatorcontrib><creatorcontrib>Sukhonpan, Kanchapan</creatorcontrib><creatorcontrib>Sayasathid, Jarun</creatorcontrib><creatorcontrib>Dean, Deborah</creatorcontrib><creatorcontrib>Kiatpathomchai, Wansika</creatorcontrib><title>Rapid and sensitive detection of Chlamydia trachomatis sexually transmitted infections in resource-constrained settings in Thailand at the point-of-care</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Infection is often asymptomatic, causing the epidemiology to be underestimated. [...]we have developed a rapid, inexpensive, easy-to-interpret, sensitive and specific point-of-care (POC) C. trachomatis detection system, using loop-mediated isothermal amplification (LAMP) for target C. trachomatis DNA amplification, followed by gold nanoparticle probe (AuNP) for colorimetric C. trachomatis specific readout. Results Optimization of LAMP-AuNP Loop primers LF and LB, which specifically locate the dumbbell products of LAMP, allow for extra amplification of the loop amplicons. [...]a sufficient amount of amplicons for detection by GE (or AuNP) were made at the shorter assay time. The results demonstrated an LOD of 22.5 copies for PCR-GE and 45 copies for LAMP-GE and LAMP-AuNP. [...]increasing the LAMP reaction incubation from 20 to 35 minutes allowed the LOD to become consistent at 11.25 copies (S4 Fig, lane 1). The colorimetric change of the LAMP-AuNP was confirmed by UV-vis spectrophotometry [7]. [...]the LAMP-AuNP has an LOD of 45 copies when incubated for 20 minutes and 11.25 copies when incubated for 35 minutes.</description><subject>Bacteria</subject><subject>Bioengineering</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Causes of</subject><subject>Chlamydia</subject><subject>Chlamydia Infections - diagnosis</subject><subject>Chlamydia Infections - microbiology</subject><subject>Chlamydia Infections - prevention & control</subject><subject>Chlamydia trachomatis</subject><subject>Chlamydia trachomatis - genetics</subject><subject>Chlamydia trachomatis - isolation & purification</subject><subject>Colorimetry</subject><subject>Condoms</subject><subject>Deoxyribonucleic acid</subject><subject>Detection</subject><subject>Diagnosis</subject><subject>Disease control</subject><subject>Disease prevention</subject><subject>Distribution</subject><subject>DNA</subject><subject>DNA 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and sensitive detection of Chlamydia trachomatis sexually transmitted infections in resource-constrained settings in Thailand at the point-of-care</title><author>Somboonna, Naraporn ; Choopara, Ilada ; Arunrut, Narong ; Sukhonpan, Kanchapan ; Sayasathid, Jarun ; Dean, Deborah ; Kiatpathomchai, Wansika</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c624t-d2ff21240e40489982c40f6ee08ed6f8b964d486c5c3dfce5c5169b10b466eef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bacteria</topic><topic>Bioengineering</topic><topic>Biology and Life Sciences</topic><topic>Biotechnology</topic><topic>Causes of</topic><topic>Chlamydia</topic><topic>Chlamydia Infections - diagnosis</topic><topic>Chlamydia Infections - microbiology</topic><topic>Chlamydia Infections - prevention & control</topic><topic>Chlamydia trachomatis</topic><topic>Chlamydia trachomatis - 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transmitted diseases</topic><topic>Spectrophotometry</topic><topic>STD</topic><topic>Thailand</topic><topic>Time Factors</topic><topic>Tropical diseases</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Somboonna, Naraporn</creatorcontrib><creatorcontrib>Choopara, Ilada</creatorcontrib><creatorcontrib>Arunrut, Narong</creatorcontrib><creatorcontrib>Sukhonpan, Kanchapan</creatorcontrib><creatorcontrib>Sayasathid, Jarun</creatorcontrib><creatorcontrib>Dean, Deborah</creatorcontrib><creatorcontrib>Kiatpathomchai, Wansika</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full 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C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid and sensitive detection of Chlamydia trachomatis sexually transmitted infections in resource-constrained settings in Thailand at the point-of-care</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2018-12-01</date><risdate>2018</risdate><volume>12</volume><issue>12</issue><spage>e0006900</spage><epage>e0006900</epage><pages>e0006900-e0006900</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>Infection is often asymptomatic, causing the epidemiology to be underestimated. [...]we have developed a rapid, inexpensive, easy-to-interpret, sensitive and specific point-of-care (POC) C. trachomatis detection system, using loop-mediated isothermal amplification (LAMP) for target C. trachomatis DNA amplification, followed by gold nanoparticle probe (AuNP) for colorimetric C. trachomatis specific readout. Results Optimization of LAMP-AuNP Loop primers LF and LB, which specifically locate the dumbbell products of LAMP, allow for extra amplification of the loop amplicons. [...]a sufficient amount of amplicons for detection by GE (or AuNP) were made at the shorter assay time. The results demonstrated an LOD of 22.5 copies for PCR-GE and 45 copies for LAMP-GE and LAMP-AuNP. [...]increasing the LAMP reaction incubation from 20 to 35 minutes allowed the LOD to become consistent at 11.25 copies (S4 Fig, lane 1). The colorimetric change of the LAMP-AuNP was confirmed by UV-vis spectrophotometry [7]. [...]the LAMP-AuNP has an LOD of 45 copies when incubated for 20 minutes and 11.25 copies when incubated for 35 minutes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30571705</pmid><doi>10.1371/journal.pntd.0006900</doi><orcidid>https://orcid.org/0000-0002-7830-3509</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2018-12, Vol.12 (12), p.e0006900-e0006900 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Public Library of Science (PLoS); PubMed Central |
| subjects | Bacteria Bioengineering Biology and Life Sciences Biotechnology Causes of Chlamydia Chlamydia Infections - diagnosis Chlamydia Infections - microbiology Chlamydia Infections - prevention & control Chlamydia trachomatis Chlamydia trachomatis - genetics Chlamydia trachomatis - isolation & purification Colorimetry Condoms Deoxyribonucleic acid Detection Diagnosis Disease control Disease prevention Distribution DNA DNA polymerase Epidemiology Female From Innovation to Application Genetic engineering Gold Hospitals Humans Incubation period Infections Male Medical laboratories Medicine and Health Sciences Nanoparticles Nucleotide sequence Optimization PCR Point-of-Care Systems Polymerase chain reaction Primers Research and Analysis Methods Sensitivity and Specificity Sexually transmitted diseases Spectrophotometry STD Thailand Time Factors Tropical diseases Womens health |
| title | Rapid and sensitive detection of Chlamydia trachomatis sexually transmitted infections in resource-constrained settings in Thailand at the point-of-care |
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