A preliminary study on the application of PspA as a carrier for group A meningococcal polysaccharide
This study aimed to explore the feasibility of pneumococcal surface protein A (PspA) as a carrier protein. Three recombinant pneumococcal surface proteins from three different clades were expressed by the prokaryotic expression system and conjugated to group A meningococcal polysaccharide (GAMP) to...
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| Veröffentlicht in: | PloS one 2019-07, Vol.14 (7), p.e0218427-e0218427 |
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| creator | Wang, Lichan Tan, Yajun Wei, Chen Zhang, Huajie Luo, Peng Zhang, Shumin Ma, Xiao |
| description | This study aimed to explore the feasibility of pneumococcal surface protein A (PspA) as a carrier protein. Three recombinant pneumococcal surface proteins from three different clades were expressed by the prokaryotic expression system and conjugated to group A meningococcal polysaccharide (GAMP) to generate three polysaccharide-protein conjugates. The conjugates, unconjugated proteins, GAMP, and GAMP-TT vaccine bulk (used as positive control) were immunized into mice, and their immune effects were assessed by the methods of enzyme-linked immunosorbent assay (ELISA), flow cytometry (FCM), and serum bactericidal assay (SBA). The results showed that the polysaccharide-protein conjugates could produce higher levels of anti-GAMP IgG titers (P < 0.05), higher ratios of Th1/Th2 (P < 0.05), and higher levels of serum bactericidal activity (P < 0.05), compared with the unconjugated GAMP. The conjugation of PspAs to GAMP also enhanced the anti-PspA responses compared with unconjugated PspAs except for PspA3. In conclusion, the results indicated that the three PspAs were appropriate carrier proteins, as demonstrated by the characteristics of T-cell dependent responses to the GAMP, and might protect against group A of epidemic cerebrospinal meningitis. |
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Three recombinant pneumococcal surface proteins from three different clades were expressed by the prokaryotic expression system and conjugated to group A meningococcal polysaccharide (GAMP) to generate three polysaccharide-protein conjugates. The conjugates, unconjugated proteins, GAMP, and GAMP-TT vaccine bulk (used as positive control) were immunized into mice, and their immune effects were assessed by the methods of enzyme-linked immunosorbent assay (ELISA), flow cytometry (FCM), and serum bactericidal assay (SBA). The results showed that the polysaccharide-protein conjugates could produce higher levels of anti-GAMP IgG titers (P < 0.05), higher ratios of Th1/Th2 (P < 0.05), and higher levels of serum bactericidal activity (P < 0.05), compared with the unconjugated GAMP. The conjugation of PspAs to GAMP also enhanced the anti-PspA responses compared with unconjugated PspAs except for PspA3. In conclusion, the results indicated that the three PspAs were appropriate carrier proteins, as demonstrated by the characteristics of T-cell dependent responses to the GAMP, and might protect against group A of epidemic cerebrospinal meningitis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0218427</identifier><identifier>PMID: 31291272</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antibodies, Bacterial - immunology ; Antigens ; Bacterial proteins ; Bacterial Proteins - immunology ; Bacterial Proteins - pharmacology ; Bactericidal activity ; Biology and Life Sciences ; Conjugates ; Conjugation ; Diphtheria ; Diphtheria toxoid ; Enzyme-linked immunosorbent assay ; Enzymes ; Epidemics ; Feasibility studies ; Female ; Flow cytometry ; Food ; Genetic aspects ; Immunity (Disease) ; Immunization ; Immunoglobulin G ; Immunoglobulin G - immunology ; Immunoglobulins ; Laboratories ; Lymphocytes T ; Medical immunity ; Medicine and Health Sciences ; Meningitis ; Meningococcal Infections - immunology ; Meningococcal Infections - prevention & control ; Meningococcal Vaccines - immunology ; Meningococcal Vaccines - pharmacology ; Mice ; Mice, Inbred BALB C ; Neisseria meningitidis - immunology ; Physiological aspects ; Polysaccharides ; Polysaccharides, Bacterial - immunology ; Polysaccharides, Bacterial - pharmacology ; Preliminary Data ; Prevention ; Properties ; Protein A ; Proteins ; PspA protein ; Quality ; Research and Analysis Methods ; Salmonella ; Serum bactericidal activity ; Streptococcus infections ; Surface protein A ; T cells ; Tetanus ; Tetanus toxoid ; Toxins ; Transport proteins ; Typhoid ; Vaccines ; Vaccines, Conjugate - immunology ; Vaccines, Conjugate - pharmacology</subject><ispartof>PloS one, 2019-07, Vol.14 (7), p.e0218427-e0218427</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Wang et al 2019 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c641t-ffdf1a9807d50f1e04260ab499fa0e5a0c29c67337de06bbc33f77939505f5813</cites><orcidid>0000-0002-5078-6393</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619668/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619668/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31291272$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Lichan</creatorcontrib><creatorcontrib>Tan, Yajun</creatorcontrib><creatorcontrib>Wei, Chen</creatorcontrib><creatorcontrib>Zhang, Huajie</creatorcontrib><creatorcontrib>Luo, Peng</creatorcontrib><creatorcontrib>Zhang, Shumin</creatorcontrib><creatorcontrib>Ma, Xiao</creatorcontrib><title>A preliminary study on the application of PspA as a carrier for group A meningococcal polysaccharide</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>This study aimed to explore the feasibility of pneumococcal surface protein A (PspA) as a carrier protein. Three recombinant pneumococcal surface proteins from three different clades were expressed by the prokaryotic expression system and conjugated to group A meningococcal polysaccharide (GAMP) to generate three polysaccharide-protein conjugates. The conjugates, unconjugated proteins, GAMP, and GAMP-TT vaccine bulk (used as positive control) were immunized into mice, and their immune effects were assessed by the methods of enzyme-linked immunosorbent assay (ELISA), flow cytometry (FCM), and serum bactericidal assay (SBA). The results showed that the polysaccharide-protein conjugates could produce higher levels of anti-GAMP IgG titers (P < 0.05), higher ratios of Th1/Th2 (P < 0.05), and higher levels of serum bactericidal activity (P < 0.05), compared with the unconjugated GAMP. The conjugation of PspAs to GAMP also enhanced the anti-PspA responses compared with unconjugated PspAs except for PspA3. In conclusion, the results indicated that the three PspAs were appropriate carrier proteins, as demonstrated by the characteristics of T-cell dependent responses to the GAMP, and might protect against group A of epidemic cerebrospinal meningitis.</description><subject>Animals</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Antigens</subject><subject>Bacterial proteins</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Bactericidal activity</subject><subject>Biology and Life Sciences</subject><subject>Conjugates</subject><subject>Conjugation</subject><subject>Diphtheria</subject><subject>Diphtheria toxoid</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzymes</subject><subject>Epidemics</subject><subject>Feasibility studies</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Food</subject><subject>Genetic aspects</subject><subject>Immunity (Disease)</subject><subject>Immunization</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunoglobulins</subject><subject>Laboratories</subject><subject>Lymphocytes T</subject><subject>Medical immunity</subject><subject>Medicine and Health Sciences</subject><subject>Meningitis</subject><subject>Meningococcal Infections - immunology</subject><subject>Meningococcal Infections - prevention & control</subject><subject>Meningococcal Vaccines - immunology</subject><subject>Meningococcal Vaccines - pharmacology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Neisseria meningitidis - immunology</subject><subject>Physiological aspects</subject><subject>Polysaccharides</subject><subject>Polysaccharides, Bacterial - immunology</subject><subject>Polysaccharides, Bacterial - pharmacology</subject><subject>Preliminary Data</subject><subject>Prevention</subject><subject>Properties</subject><subject>Protein A</subject><subject>Proteins</subject><subject>PspA protein</subject><subject>Quality</subject><subject>Research and Analysis Methods</subject><subject>Salmonella</subject><subject>Serum bactericidal activity</subject><subject>Streptococcus infections</subject><subject>Surface protein A</subject><subject>T cells</subject><subject>Tetanus</subject><subject>Tetanus toxoid</subject><subject>Toxins</subject><subject>Transport proteins</subject><subject>Typhoid</subject><subject>Vaccines</subject><subject>Vaccines, Conjugate - immunology</subject><subject>Vaccines, Conjugate - pharmacology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1uL1DAUx4so7jr6DUQDgujDjLm0afMiDIuXgYUVb6_hNE06WTJNN2nF-fZmnO4ylX2QPOT2O_-Tc3JOlj0neEVYSd5d-zF04Fa97_QKU1LltHyQnRPB6JJTzB6erM-yJzFeY1ywivPH2RkjVBBa0vOsWaM-aGd3toOwR3EYmz3yHRq2GkHfO6tgsGnvDfoS-zWCiAApCMHqgIwPqA1-7NEa7XRnu9YrrxQ41Hu3j6DUFoJt9NPskQEX9bNpXmQ_Pn74fvF5eXn1aXOxvlwqnpNhaUxjCIgKl02BDdE4pxxDnQthAOsCsKJC8ZKxstGY17VizJSlYKLAhSkqwhbZy6Nu73yUU4KipLTglORlCn-RbY5E4-Fa9sHuUtTSg5V_D3xoJYTBKqdlWdG6ZDnUnPNc6Ubg5E0TQRgmdXpH0no_eRvrnW6U7oYAbiY6v-nsVrb-l-ScCM6rJPBmEgj-ZtRxkDsblXYOOu3H47sJ5gyLhL76B70_uolqIQVgO-OTX3UQletC0IoX6eMTtbqHSqPRO6tSNRmbzmcGb2cGiRn076GFMUa5-fb1_9mrn3P29Qm71eCGbfRuPBRcnIP5EVTBxxi0uUsywfLQDLfZkIdmkFMzJLMXpx90Z3Rb_ewPQmsDZQ</recordid><startdate>20190710</startdate><enddate>20190710</enddate><creator>Wang, Lichan</creator><creator>Tan, Yajun</creator><creator>Wei, Chen</creator><creator>Zhang, Huajie</creator><creator>Luo, Peng</creator><creator>Zhang, Shumin</creator><creator>Ma, Xiao</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-5078-6393</orcidid></search><sort><creationdate>20190710</creationdate><title>A preliminary study on the application of PspA as a carrier for group A meningococcal polysaccharide</title><author>Wang, Lichan ; Tan, Yajun ; Wei, Chen ; Zhang, Huajie ; Luo, Peng ; Zhang, Shumin ; Ma, Xiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c641t-ffdf1a9807d50f1e04260ab499fa0e5a0c29c67337de06bbc33f77939505f5813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - immunology</topic><topic>Antigens</topic><topic>Bacterial proteins</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacterial Proteins - pharmacology</topic><topic>Bactericidal activity</topic><topic>Biology and Life Sciences</topic><topic>Conjugates</topic><topic>Conjugation</topic><topic>Diphtheria</topic><topic>Diphtheria toxoid</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzymes</topic><topic>Epidemics</topic><topic>Feasibility studies</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Food</topic><topic>Genetic aspects</topic><topic>Immunity (Disease)</topic><topic>Immunization</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin G - immunology</topic><topic>Immunoglobulins</topic><topic>Laboratories</topic><topic>Lymphocytes T</topic><topic>Medical immunity</topic><topic>Medicine and Health Sciences</topic><topic>Meningitis</topic><topic>Meningococcal Infections - immunology</topic><topic>Meningococcal Infections - prevention & control</topic><topic>Meningococcal Vaccines - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Lichan</au><au>Tan, Yajun</au><au>Wei, Chen</au><au>Zhang, Huajie</au><au>Luo, Peng</au><au>Zhang, Shumin</au><au>Ma, Xiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A preliminary study on the application of PspA as a carrier for group A meningococcal polysaccharide</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-07-10</date><risdate>2019</risdate><volume>14</volume><issue>7</issue><spage>e0218427</spage><epage>e0218427</epage><pages>e0218427-e0218427</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This study aimed to explore the feasibility of pneumococcal surface protein A (PspA) as a carrier protein. Three recombinant pneumococcal surface proteins from three different clades were expressed by the prokaryotic expression system and conjugated to group A meningococcal polysaccharide (GAMP) to generate three polysaccharide-protein conjugates. The conjugates, unconjugated proteins, GAMP, and GAMP-TT vaccine bulk (used as positive control) were immunized into mice, and their immune effects were assessed by the methods of enzyme-linked immunosorbent assay (ELISA), flow cytometry (FCM), and serum bactericidal assay (SBA). The results showed that the polysaccharide-protein conjugates could produce higher levels of anti-GAMP IgG titers (P < 0.05), higher ratios of Th1/Th2 (P < 0.05), and higher levels of serum bactericidal activity (P < 0.05), compared with the unconjugated GAMP. The conjugation of PspAs to GAMP also enhanced the anti-PspA responses compared with unconjugated PspAs except for PspA3. In conclusion, the results indicated that the three PspAs were appropriate carrier proteins, as demonstrated by the characteristics of T-cell dependent responses to the GAMP, and might protect against group A of epidemic cerebrospinal meningitis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31291272</pmid><doi>10.1371/journal.pone.0218427</doi><tpages>e0218427</tpages><orcidid>https://orcid.org/0000-0002-5078-6393</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animals Antibodies, Bacterial - immunology Antigens Bacterial proteins Bacterial Proteins - immunology Bacterial Proteins - pharmacology Bactericidal activity Biology and Life Sciences Conjugates Conjugation Diphtheria Diphtheria toxoid Enzyme-linked immunosorbent assay Enzymes Epidemics Feasibility studies Female Flow cytometry Food Genetic aspects Immunity (Disease) Immunization Immunoglobulin G Immunoglobulin G - immunology Immunoglobulins Laboratories Lymphocytes T Medical immunity Medicine and Health Sciences Meningitis Meningococcal Infections - immunology Meningococcal Infections - prevention & control Meningococcal Vaccines - immunology Meningococcal Vaccines - pharmacology Mice Mice, Inbred BALB C Neisseria meningitidis - immunology Physiological aspects Polysaccharides Polysaccharides, Bacterial - immunology Polysaccharides, Bacterial - pharmacology Preliminary Data Prevention Properties Protein A Proteins PspA protein Quality Research and Analysis Methods Salmonella Serum bactericidal activity Streptococcus infections Surface protein A T cells Tetanus Tetanus toxoid Toxins Transport proteins Typhoid Vaccines Vaccines, Conjugate - immunology Vaccines, Conjugate - pharmacology |
| title | A preliminary study on the application of PspA as a carrier for group A meningococcal polysaccharide |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T13%3A39%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20preliminary%20study%20on%20the%20application%20of%20PspA%20as%20a%20carrier%20for%20group%20A%20meningococcal%20polysaccharide&rft.jtitle=PloS%20one&rft.au=Wang,%20Lichan&rft.date=2019-07-10&rft.volume=14&rft.issue=7&rft.spage=e0218427&rft.epage=e0218427&rft.pages=e0218427-e0218427&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0218427&rft_dat=%3Cgale_plos_%3EA592865312%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2256214705&rft_id=info:pmid/31291272&rft_galeid=A592865312&rft_doaj_id=oai_doaj_org_article_782b734ab6664ced903f7e191301b9c6&rfr_iscdi=true |