Seroprevalence, cross antigenicity and circulation sphere of bat-borne hantaviruses revealed by serological and antigenic analyses
Bats are newly identified reservoirs of hantaviruses (HVs) among which very divergent HVs have been discovered in recent years. However, their significance for public health remains unclear since their seroprevalence as well as antigenic relationship with human-infecting HVs have not been investigat...
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creator | Xu, Lin Wu, Jianmin Li, Qi Wei, Yamei Tan, Zhizhou Cai, Jianqiu Guo, Huancheng Yang, Ling'en Huang, Xiaohong Chen, Jing Zhang, Fuqiang He, Biao Tu, Changchun |
description | Bats are newly identified reservoirs of hantaviruses (HVs) among which very divergent HVs have been discovered in recent years. However, their significance for public health remains unclear since their seroprevalence as well as antigenic relationship with human-infecting HVs have not been investigated. In the present study archived tissues of 1,419 bats of 22 species from 6 families collected in 5 south and southwest provinces in China were screened by pan-HV RT-PCR following viral metagenomic analysis. As a result nine HVs have been identified in two bat species in two provinces and phylogenetically classified into two species, Laibin virus (LAIV, ICTV approved species, 1 strain) and Xuan son virus (XSV, proposed species, 8 strains). Additionally, 709 serum samples of these bats were also analyzed by ELISA to investigate the seroprevalence and cross-reactivity between different HVs using expressed recombinant nucleocapsid proteins (rNPs) of LAIV, XSV and Seoul virus (SEOV). The cross-reactivity of some bat sera were further confirmed by western blot (WB) using three rNPs followed by fluorescent antibody virus neutralization test (FAVNT) against live SEOV. Results showed that the total HV seropositive rate of bat sera was 18.5% (131/709) with many cross reacting with two or all three rNPs and several able to neutralize SEOV. WB analysis using the three rNPs and their specific hyperimmune sera demonstrated cross-reactivity between XSV/SEOV and LAIV/XSV, but not LAIV/SEOV, indicating that XSV is antigenically closer to human-infecting HVs. In addition a study of the distribution of the viruses identified an area covering the region between Chinese Guangxi and North Vietnam, in which XSV and LAIV circulate within different bat colonies with a high seroprevalence. A circulation sphere of bat-borne HVs has therefore been proposed. |
doi_str_mv | 10.1371/journal.ppat.1007545 |
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However, their significance for public health remains unclear since their seroprevalence as well as antigenic relationship with human-infecting HVs have not been investigated. In the present study archived tissues of 1,419 bats of 22 species from 6 families collected in 5 south and southwest provinces in China were screened by pan-HV RT-PCR following viral metagenomic analysis. As a result nine HVs have been identified in two bat species in two provinces and phylogenetically classified into two species, Laibin virus (LAIV, ICTV approved species, 1 strain) and Xuan son virus (XSV, proposed species, 8 strains). Additionally, 709 serum samples of these bats were also analyzed by ELISA to investigate the seroprevalence and cross-reactivity between different HVs using expressed recombinant nucleocapsid proteins (rNPs) of LAIV, XSV and Seoul virus (SEOV). The cross-reactivity of some bat sera were further confirmed by western blot (WB) using three rNPs followed by fluorescent antibody virus neutralization test (FAVNT) against live SEOV. Results showed that the total HV seropositive rate of bat sera was 18.5% (131/709) with many cross reacting with two or all three rNPs and several able to neutralize SEOV. WB analysis using the three rNPs and their specific hyperimmune sera demonstrated cross-reactivity between XSV/SEOV and LAIV/XSV, but not LAIV/SEOV, indicating that XSV is antigenically closer to human-infecting HVs. In addition a study of the distribution of the viruses identified an area covering the region between Chinese Guangxi and North Vietnam, in which XSV and LAIV circulate within different bat colonies with a high seroprevalence. A circulation sphere of bat-borne HVs has therefore been proposed.</description><identifier>ISSN: 1553-7374</identifier><identifier>ISSN: 1553-7366</identifier><identifier>EISSN: 1553-7374</identifier><identifier>DOI: 10.1371/journal.ppat.1007545</identifier><identifier>PMID: 30668611</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibodies ; Antigenic relationship ; Antigenicity ; Antigens ; Bats ; Bats (Animals) ; Biology and Life Sciences ; Chiroptera ; Computer and Information Sciences ; Cross-reactivity ; Disease prevention ; Distribution ; Enzyme-linked immunosorbent assay ; Fluorescence ; Genetic diversity ; Genomes ; Hantaviruses ; Infectious diseases ; Laboratories ; Medicine and Health Sciences ; Neutralization ; Nucleocapsids ; People and Places ; Phylogenetics ; Phylogeny ; Polymerase chain reaction ; Prevalence studies (Epidemiology) ; Proteins ; Public health ; Reactivity ; Research and Analysis Methods ; RNA polymerase ; Serology ; Species ; Species classification ; Veterinary medicine ; Virology ; Viruses ; Zoonoses</subject><ispartof>PLoS pathogens, 2019-01, Vol.15 (1), p.e1007545-e1007545</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Xu et al 2019 Xu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c661t-2ef2d26ff5e836b645fc26c5ebb31682325eb9123178542660bfb4433e9986b03</citedby><cites>FETCH-LOGICAL-c661t-2ef2d26ff5e836b645fc26c5ebb31682325eb9123178542660bfb4433e9986b03</cites><orcidid>0000-0002-5322-1435 ; 0000-0002-2844-9673 ; 0000-0002-8134-7502 ; 0000-0001-6215-7888 ; 0000-0001-7587-152X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358112/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358112/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23864,27922,27923,53789,53791,79370,79371</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30668611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Klein, Sabra L.</contributor><creatorcontrib>Xu, Lin</creatorcontrib><creatorcontrib>Wu, Jianmin</creatorcontrib><creatorcontrib>Li, Qi</creatorcontrib><creatorcontrib>Wei, Yamei</creatorcontrib><creatorcontrib>Tan, Zhizhou</creatorcontrib><creatorcontrib>Cai, Jianqiu</creatorcontrib><creatorcontrib>Guo, Huancheng</creatorcontrib><creatorcontrib>Yang, Ling'en</creatorcontrib><creatorcontrib>Huang, Xiaohong</creatorcontrib><creatorcontrib>Chen, Jing</creatorcontrib><creatorcontrib>Zhang, Fuqiang</creatorcontrib><creatorcontrib>He, Biao</creatorcontrib><creatorcontrib>Tu, Changchun</creatorcontrib><title>Seroprevalence, cross antigenicity and circulation sphere of bat-borne hantaviruses revealed by serological and antigenic analyses</title><title>PLoS pathogens</title><addtitle>PLoS Pathog</addtitle><description>Bats are newly identified reservoirs of hantaviruses (HVs) among which very divergent HVs have been discovered in recent years. However, their significance for public health remains unclear since their seroprevalence as well as antigenic relationship with human-infecting HVs have not been investigated. In the present study archived tissues of 1,419 bats of 22 species from 6 families collected in 5 south and southwest provinces in China were screened by pan-HV RT-PCR following viral metagenomic analysis. As a result nine HVs have been identified in two bat species in two provinces and phylogenetically classified into two species, Laibin virus (LAIV, ICTV approved species, 1 strain) and Xuan son virus (XSV, proposed species, 8 strains). Additionally, 709 serum samples of these bats were also analyzed by ELISA to investigate the seroprevalence and cross-reactivity between different HVs using expressed recombinant nucleocapsid proteins (rNPs) of LAIV, XSV and Seoul virus (SEOV). The cross-reactivity of some bat sera were further confirmed by western blot (WB) using three rNPs followed by fluorescent antibody virus neutralization test (FAVNT) against live SEOV. Results showed that the total HV seropositive rate of bat sera was 18.5% (131/709) with many cross reacting with two or all three rNPs and several able to neutralize SEOV. WB analysis using the three rNPs and their specific hyperimmune sera demonstrated cross-reactivity between XSV/SEOV and LAIV/XSV, but not LAIV/SEOV, indicating that XSV is antigenically closer to human-infecting HVs. In addition a study of the distribution of the viruses identified an area covering the region between Chinese Guangxi and North Vietnam, in which XSV and LAIV circulate within different bat colonies with a high seroprevalence. A circulation sphere of bat-borne HVs has therefore been proposed.</description><subject>Antibodies</subject><subject>Antigenic relationship</subject><subject>Antigenicity</subject><subject>Antigens</subject><subject>Bats</subject><subject>Bats (Animals)</subject><subject>Biology and Life Sciences</subject><subject>Chiroptera</subject><subject>Computer and Information Sciences</subject><subject>Cross-reactivity</subject><subject>Disease prevention</subject><subject>Distribution</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Fluorescence</subject><subject>Genetic diversity</subject><subject>Genomes</subject><subject>Hantaviruses</subject><subject>Infectious diseases</subject><subject>Laboratories</subject><subject>Medicine and Health Sciences</subject><subject>Neutralization</subject><subject>Nucleocapsids</subject><subject>People and Places</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Prevalence studies (Epidemiology)</subject><subject>Proteins</subject><subject>Public health</subject><subject>Reactivity</subject><subject>Research and Analysis Methods</subject><subject>RNA polymerase</subject><subject>Serology</subject><subject>Species</subject><subject>Species classification</subject><subject>Veterinary 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cross antigenicity and circulation sphere of bat-borne hantaviruses revealed by serological and antigenic analyses</title><author>Xu, Lin ; Wu, Jianmin ; Li, Qi ; Wei, Yamei ; Tan, Zhizhou ; Cai, Jianqiu ; Guo, Huancheng ; Yang, Ling'en ; Huang, Xiaohong ; Chen, Jing ; Zhang, Fuqiang ; He, Biao ; Tu, Changchun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c661t-2ef2d26ff5e836b645fc26c5ebb31682325eb9123178542660bfb4433e9986b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibodies</topic><topic>Antigenic relationship</topic><topic>Antigenicity</topic><topic>Antigens</topic><topic>Bats</topic><topic>Bats (Animals)</topic><topic>Biology and Life Sciences</topic><topic>Chiroptera</topic><topic>Computer and Information Sciences</topic><topic>Cross-reactivity</topic><topic>Disease prevention</topic><topic>Distribution</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Fluorescence</topic><topic>Genetic diversity</topic><topic>Genomes</topic><topic>Hantaviruses</topic><topic>Infectious diseases</topic><topic>Laboratories</topic><topic>Medicine and Health Sciences</topic><topic>Neutralization</topic><topic>Nucleocapsids</topic><topic>People and Places</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Prevalence studies (Epidemiology)</topic><topic>Proteins</topic><topic>Public health</topic><topic>Reactivity</topic><topic>Research and Analysis Methods</topic><topic>RNA polymerase</topic><topic>Serology</topic><topic>Species</topic><topic>Species classification</topic><topic>Veterinary medicine</topic><topic>Virology</topic><topic>Viruses</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Lin</creatorcontrib><creatorcontrib>Wu, Jianmin</creatorcontrib><creatorcontrib>Li, 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Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Lin</au><au>Wu, Jianmin</au><au>Li, Qi</au><au>Wei, Yamei</au><au>Tan, Zhizhou</au><au>Cai, Jianqiu</au><au>Guo, Huancheng</au><au>Yang, Ling'en</au><au>Huang, Xiaohong</au><au>Chen, Jing</au><au>Zhang, Fuqiang</au><au>He, Biao</au><au>Tu, Changchun</au><au>Klein, Sabra L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Seroprevalence, cross antigenicity and circulation sphere of bat-borne hantaviruses revealed by serological and antigenic analyses</atitle><jtitle>PLoS pathogens</jtitle><addtitle>PLoS Pathog</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>15</volume><issue>1</issue><spage>e1007545</spage><epage>e1007545</epage><pages>e1007545-e1007545</pages><issn>1553-7374</issn><issn>1553-7366</issn><eissn>1553-7374</eissn><abstract>Bats are newly identified reservoirs of hantaviruses (HVs) among which very divergent HVs have been discovered in recent years. However, their significance for public health remains unclear since their seroprevalence as well as antigenic relationship with human-infecting HVs have not been investigated. In the present study archived tissues of 1,419 bats of 22 species from 6 families collected in 5 south and southwest provinces in China were screened by pan-HV RT-PCR following viral metagenomic analysis. As a result nine HVs have been identified in two bat species in two provinces and phylogenetically classified into two species, Laibin virus (LAIV, ICTV approved species, 1 strain) and Xuan son virus (XSV, proposed species, 8 strains). Additionally, 709 serum samples of these bats were also analyzed by ELISA to investigate the seroprevalence and cross-reactivity between different HVs using expressed recombinant nucleocapsid proteins (rNPs) of LAIV, XSV and Seoul virus (SEOV). The cross-reactivity of some bat sera were further confirmed by western blot (WB) using three rNPs followed by fluorescent antibody virus neutralization test (FAVNT) against live SEOV. Results showed that the total HV seropositive rate of bat sera was 18.5% (131/709) with many cross reacting with two or all three rNPs and several able to neutralize SEOV. WB analysis using the three rNPs and their specific hyperimmune sera demonstrated cross-reactivity between XSV/SEOV and LAIV/XSV, but not LAIV/SEOV, indicating that XSV is antigenically closer to human-infecting HVs. In addition a study of the distribution of the viruses identified an area covering the region between Chinese Guangxi and North Vietnam, in which XSV and LAIV circulate within different bat colonies with a high seroprevalence. A circulation sphere of bat-borne HVs has therefore been proposed.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30668611</pmid><doi>10.1371/journal.ppat.1007545</doi><orcidid>https://orcid.org/0000-0002-5322-1435</orcidid><orcidid>https://orcid.org/0000-0002-2844-9673</orcidid><orcidid>https://orcid.org/0000-0002-8134-7502</orcidid><orcidid>https://orcid.org/0000-0001-6215-7888</orcidid><orcidid>https://orcid.org/0000-0001-7587-152X</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Antibodies Antigenic relationship Antigenicity Antigens Bats Bats (Animals) Biology and Life Sciences Chiroptera Computer and Information Sciences Cross-reactivity Disease prevention Distribution Enzyme-linked immunosorbent assay Fluorescence Genetic diversity Genomes Hantaviruses Infectious diseases Laboratories Medicine and Health Sciences Neutralization Nucleocapsids People and Places Phylogenetics Phylogeny Polymerase chain reaction Prevalence studies (Epidemiology) Proteins Public health Reactivity Research and Analysis Methods RNA polymerase Serology Species Species classification Veterinary medicine Virology Viruses Zoonoses |
title | Seroprevalence, cross antigenicity and circulation sphere of bat-borne hantaviruses revealed by serological and antigenic analyses |
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