Fretibacterium sp. human oral taxon 360 is a novel biomarker for periodontitis screening in the Japanese population
Periodontitis is a common inflammatory disease, leading to bone destruction and tooth loss. Screening for periodontitis is important in preventing the progress of this disease. Various types of bacteria have been examined as potential screening targets, but only culturable pathogenic bacteria have b...
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| Veröffentlicht in: | PloS one 2019-06, Vol.14 (6), p.e0218266-e0218266 |
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| creator | Khemwong, Thatawee Kobayashi, Hiroaki Ikeda, Yuichi Matsuura, Takanori Sudo, Takeaki Kano, Chihiro Mikami, Ryo Izumi, Yuichi |
| description | Periodontitis is a common inflammatory disease, leading to bone destruction and tooth loss. Screening for periodontitis is important in preventing the progress of this disease. Various types of bacteria have been examined as potential screening targets, but only culturable pathogenic bacteria have been considered candidates. Recently, the various uncultivable bacteria have been identified in microbiome studies, but the value of these bacteria in periodontitis screening remains unknown.
The aim of this study was to evaluate the diagnostic use of uncultivable bacteria Fretibacterium sp. HOT 360 and TM7 sp. HOT 356 for periodontitis screening in the Japanese population.
Stimulated saliva samples were collected from 217 participants (periodontitis group, n = 157; healthy group, n = 60). The two uncultivable bacterial species selected were: Fretibacterium sp. human oral taxon 360 (Fretibacterium sp. HOT 360) and TM7 sp. human oral taxon 356 (TM7 sp. HOT 356). The levels of these two bacterial species were compared with those of Porphyromonas gingivalis (P. gingivalis), a keystone pathogen in periodontitis. These three species of bacteria were then quantified using qualitative real-time polymerase chain reaction (qPCR) with specific primers and Taqman probes. Statistical analysis was performed by SPSS 20.0 software. P value was statistically significant at .05.
The populations of uncultivable bacterial species TM7 sp. HOT 356 and Fretibacterium sp. HOT 360 were significantly higher in periodontitis group than in healthy group. Only Fretibacterium sp. HOT 360 showed a significantly positive correlation with such periodontal parameters as probing pocket depth (PPD) and bleeding on probing (BOP).
These findings indicate that uncultivable bacteria Fretibacterium sp. HOT 360 can be used as a saliva-based diagnostic bacterial biomarker for periodontitis screening. |
| doi_str_mv | 10.1371/journal.pone.0218266 |
| format | Article |
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The aim of this study was to evaluate the diagnostic use of uncultivable bacteria Fretibacterium sp. HOT 360 and TM7 sp. HOT 356 for periodontitis screening in the Japanese population.
Stimulated saliva samples were collected from 217 participants (periodontitis group, n = 157; healthy group, n = 60). The two uncultivable bacterial species selected were: Fretibacterium sp. human oral taxon 360 (Fretibacterium sp. HOT 360) and TM7 sp. human oral taxon 356 (TM7 sp. HOT 356). The levels of these two bacterial species were compared with those of Porphyromonas gingivalis (P. gingivalis), a keystone pathogen in periodontitis. These three species of bacteria were then quantified using qualitative real-time polymerase chain reaction (qPCR) with specific primers and Taqman probes. Statistical analysis was performed by SPSS 20.0 software. P value was statistically significant at .05.
The populations of uncultivable bacterial species TM7 sp. HOT 356 and Fretibacterium sp. HOT 360 were significantly higher in periodontitis group than in healthy group. Only Fretibacterium sp. HOT 360 showed a significantly positive correlation with such periodontal parameters as probing pocket depth (PPD) and bleeding on probing (BOP).
These findings indicate that uncultivable bacteria Fretibacterium sp. HOT 360 can be used as a saliva-based diagnostic bacterial biomarker for periodontitis screening.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0218266</identifier><identifier>PMID: 31216300</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aged ; Bacteria ; Bacteroides - genetics ; Biofilms - growth & development ; Bioindicators ; Biological markers ; Biology and Life Sciences ; Biomarkers ; Biomarkers - analysis ; Bleeding ; Bone loss ; Dental Plaque - diagnosis ; Dental Plaque - microbiology ; Diagnosis ; Diagnostic systems ; Disease ; Female ; Gene expression ; Gum disease ; Health screening ; Humans ; Inflammatory diseases ; Japan - epidemiology ; Male ; Medicine and Health Sciences ; Microbiomes ; Microbiota ; Microbiota - genetics ; Middle Aged ; Oral hygiene ; Periodontitis ; Periodontitis - diagnosis ; Periodontitis - microbiology ; Polymerase chain reaction ; Population (statistical) ; Porphyromonas gingivalis - pathogenicity ; Primers ; Saliva ; Saliva - microbiology ; Screening ; Smoking ; Social Sciences ; Species ; Statistical analysis ; Statistical methods ; Taxa ; Teeth ; Tooth loss</subject><ispartof>PloS one, 2019-06, Vol.14 (6), p.e0218266-e0218266</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Khemwong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Khemwong et al 2019 Khemwong et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-53069af0428282bf3877b8b0a16bb7697b038789a4d30f1dbab50fc0c80d90783</citedby><cites>FETCH-LOGICAL-c758t-53069af0428282bf3877b8b0a16bb7697b038789a4d30f1dbab50fc0c80d90783</cites><orcidid>0000-0001-9481-5013</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584019/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584019/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31216300$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ojcius, David M.</contributor><creatorcontrib>Khemwong, Thatawee</creatorcontrib><creatorcontrib>Kobayashi, Hiroaki</creatorcontrib><creatorcontrib>Ikeda, Yuichi</creatorcontrib><creatorcontrib>Matsuura, Takanori</creatorcontrib><creatorcontrib>Sudo, Takeaki</creatorcontrib><creatorcontrib>Kano, Chihiro</creatorcontrib><creatorcontrib>Mikami, Ryo</creatorcontrib><creatorcontrib>Izumi, Yuichi</creatorcontrib><title>Fretibacterium sp. human oral taxon 360 is a novel biomarker for periodontitis screening in the Japanese population</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Periodontitis is a common inflammatory disease, leading to bone destruction and tooth loss. Screening for periodontitis is important in preventing the progress of this disease. Various types of bacteria have been examined as potential screening targets, but only culturable pathogenic bacteria have been considered candidates. Recently, the various uncultivable bacteria have been identified in microbiome studies, but the value of these bacteria in periodontitis screening remains unknown.
The aim of this study was to evaluate the diagnostic use of uncultivable bacteria Fretibacterium sp. HOT 360 and TM7 sp. HOT 356 for periodontitis screening in the Japanese population.
Stimulated saliva samples were collected from 217 participants (periodontitis group, n = 157; healthy group, n = 60). The two uncultivable bacterial species selected were: Fretibacterium sp. human oral taxon 360 (Fretibacterium sp. HOT 360) and TM7 sp. human oral taxon 356 (TM7 sp. HOT 356). The levels of these two bacterial species were compared with those of Porphyromonas gingivalis (P. gingivalis), a keystone pathogen in periodontitis. These three species of bacteria were then quantified using qualitative real-time polymerase chain reaction (qPCR) with specific primers and Taqman probes. Statistical analysis was performed by SPSS 20.0 software. P value was statistically significant at .05.
The populations of uncultivable bacterial species TM7 sp. HOT 356 and Fretibacterium sp. HOT 360 were significantly higher in periodontitis group than in healthy group. Only Fretibacterium sp. HOT 360 showed a significantly positive correlation with such periodontal parameters as probing pocket depth (PPD) and bleeding on probing (BOP).
These findings indicate that uncultivable bacteria Fretibacterium sp. HOT 360 can be used as a saliva-based diagnostic bacterial biomarker for periodontitis screening.</description><subject>Aged</subject><subject>Bacteria</subject><subject>Bacteroides - genetics</subject><subject>Biofilms - growth & development</subject><subject>Bioindicators</subject><subject>Biological markers</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biomarkers - analysis</subject><subject>Bleeding</subject><subject>Bone loss</subject><subject>Dental Plaque - diagnosis</subject><subject>Dental Plaque - microbiology</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>Disease</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gum disease</subject><subject>Health screening</subject><subject>Humans</subject><subject>Inflammatory diseases</subject><subject>Japan - epidemiology</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>Microbiota - genetics</subject><subject>Middle Aged</subject><subject>Oral hygiene</subject><subject>Periodontitis</subject><subject>Periodontitis - diagnosis</subject><subject>Periodontitis - microbiology</subject><subject>Polymerase chain reaction</subject><subject>Population (statistical)</subject><subject>Porphyromonas gingivalis - pathogenicity</subject><subject>Primers</subject><subject>Saliva</subject><subject>Saliva - microbiology</subject><subject>Screening</subject><subject>Smoking</subject><subject>Social Sciences</subject><subject>Species</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>Taxa</subject><subject>Teeth</subject><subject>Tooth loss</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11vFCEUhidGY-vqPzBKYmL0YldmYIC5MWkaq2uaNPHrlgADu9RZmALT1H8v0502O6YXhgvI4Tnv-YBTFC9LuCoRLT9c-iE40a167_QKViWrCHlUHJcNqpakgujxwfmoeBbjJYQ1YoQ8LY5QWZUEQXhcxLOgk5VCJR3ssAOxX4HtsBMO-CA6kMSNdwARCGwEAjh_rTsgrd-J8FsHYHwAfXb0rXfJpsxEFbR21m2AdSBtNfgqeuF01KD3_dCJZL17Xjwxoov6xbQvip9nn36cflmeX3xen56cLxWtWVrWCJJGGIgrlpc0iFEqmYSiJFJS0lAJs4k1ArcImrKVQtbQKKgYbBtIGVoUr_e6fecjn_oVeVVhhAmu8Uis90TrxSXvg811_eFeWH5r8GHDRUhWdZobUdFaNjkFzDBWpmk0a3MGSBtZ01pkrY9TtEHudKu0S7mDM9H5jbNbvvHXnNQMw_xUi-LdJBD81aBj4jsble663D8_3OaNSwQZrTP65h_04eomaiNyAdYZn-OqUZSf1KyhGNNqDLt6gMqr1Tur8ucyNttnDu9nDplJ-iZtxBAjX3__9v_sxa85-_aA3WrRpW303TB-mTgH8R5UwccYtLlvcgn5OBt33eDjbPBpNrLbq8MHune6Gwb0F4bCCYw</recordid><startdate>20190619</startdate><enddate>20190619</enddate><creator>Khemwong, Thatawee</creator><creator>Kobayashi, Hiroaki</creator><creator>Ikeda, Yuichi</creator><creator>Matsuura, Takanori</creator><creator>Sudo, Takeaki</creator><creator>Kano, Chihiro</creator><creator>Mikami, Ryo</creator><creator>Izumi, Yuichi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-9481-5013</orcidid></search><sort><creationdate>20190619</creationdate><title>Fretibacterium sp. human oral taxon 360 is a novel biomarker for periodontitis screening in the Japanese population</title><author>Khemwong, Thatawee ; Kobayashi, Hiroaki ; Ikeda, Yuichi ; Matsuura, Takanori ; Sudo, Takeaki ; Kano, Chihiro ; Mikami, Ryo ; Izumi, Yuichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-53069af0428282bf3877b8b0a16bb7697b038789a4d30f1dbab50fc0c80d90783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aged</topic><topic>Bacteria</topic><topic>Bacteroides - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khemwong, Thatawee</au><au>Kobayashi, Hiroaki</au><au>Ikeda, Yuichi</au><au>Matsuura, Takanori</au><au>Sudo, Takeaki</au><au>Kano, Chihiro</au><au>Mikami, Ryo</au><au>Izumi, Yuichi</au><au>Ojcius, David M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fretibacterium sp. human oral taxon 360 is a novel biomarker for periodontitis screening in the Japanese population</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-06-19</date><risdate>2019</risdate><volume>14</volume><issue>6</issue><spage>e0218266</spage><epage>e0218266</epage><pages>e0218266-e0218266</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Periodontitis is a common inflammatory disease, leading to bone destruction and tooth loss. Screening for periodontitis is important in preventing the progress of this disease. Various types of bacteria have been examined as potential screening targets, but only culturable pathogenic bacteria have been considered candidates. Recently, the various uncultivable bacteria have been identified in microbiome studies, but the value of these bacteria in periodontitis screening remains unknown.
The aim of this study was to evaluate the diagnostic use of uncultivable bacteria Fretibacterium sp. HOT 360 and TM7 sp. HOT 356 for periodontitis screening in the Japanese population.
Stimulated saliva samples were collected from 217 participants (periodontitis group, n = 157; healthy group, n = 60). The two uncultivable bacterial species selected were: Fretibacterium sp. human oral taxon 360 (Fretibacterium sp. HOT 360) and TM7 sp. human oral taxon 356 (TM7 sp. HOT 356). The levels of these two bacterial species were compared with those of Porphyromonas gingivalis (P. gingivalis), a keystone pathogen in periodontitis. These three species of bacteria were then quantified using qualitative real-time polymerase chain reaction (qPCR) with specific primers and Taqman probes. Statistical analysis was performed by SPSS 20.0 software. P value was statistically significant at .05.
The populations of uncultivable bacterial species TM7 sp. HOT 356 and Fretibacterium sp. HOT 360 were significantly higher in periodontitis group than in healthy group. Only Fretibacterium sp. HOT 360 showed a significantly positive correlation with such periodontal parameters as probing pocket depth (PPD) and bleeding on probing (BOP).
These findings indicate that uncultivable bacteria Fretibacterium sp. HOT 360 can be used as a saliva-based diagnostic bacterial biomarker for periodontitis screening.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31216300</pmid><doi>10.1371/journal.pone.0218266</doi><tpages>e0218266</tpages><orcidid>https://orcid.org/0000-0001-9481-5013</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_2243464548 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aged Bacteria Bacteroides - genetics Biofilms - growth & development Bioindicators Biological markers Biology and Life Sciences Biomarkers Biomarkers - analysis Bleeding Bone loss Dental Plaque - diagnosis Dental Plaque - microbiology Diagnosis Diagnostic systems Disease Female Gene expression Gum disease Health screening Humans Inflammatory diseases Japan - epidemiology Male Medicine and Health Sciences Microbiomes Microbiota Microbiota - genetics Middle Aged Oral hygiene Periodontitis Periodontitis - diagnosis Periodontitis - microbiology Polymerase chain reaction Population (statistical) Porphyromonas gingivalis - pathogenicity Primers Saliva Saliva - microbiology Screening Smoking Social Sciences Species Statistical analysis Statistical methods Taxa Teeth Tooth loss |
| title | Fretibacterium sp. human oral taxon 360 is a novel biomarker for periodontitis screening in the Japanese population |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T08%3A55%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fretibacterium%20sp.%20human%20oral%20taxon%20360%20is%20a%20novel%20biomarker%20for%20periodontitis%20screening%20in%20the%20Japanese%20population&rft.jtitle=PloS%20one&rft.au=Khemwong,%20Thatawee&rft.date=2019-06-19&rft.volume=14&rft.issue=6&rft.spage=e0218266&rft.epage=e0218266&rft.pages=e0218266-e0218266&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0218266&rft_dat=%3Cgale_plos_%3EA589744729%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2243464548&rft_id=info:pmid/31216300&rft_galeid=A589744729&rft_doaj_id=oai_doaj_org_article_fa275b977b4844cf99e8db033efb575a&rfr_iscdi=true |