Analysis of complexes formed by small gold nanoparticles in low concentration in cell culture media
New nanomaterials are constantly developed with applications in everything from cosmetics to high tech electronics. Assessing their biological impact has been done by analysis of their adsorbed protein corona, in vitro cell assays, and larger scale ecotoxicological studies. This has proved to be a h...
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| creator | Gunnarsson, Stefán B Bernfur, Katja Englund-Johansson, Ulrica Johansson, Fredrik Cedervall, Tommy |
| description | New nanomaterials are constantly developed with applications in everything from cosmetics to high tech electronics. Assessing their biological impact has been done by analysis of their adsorbed protein corona, in vitro cell assays, and larger scale ecotoxicological studies. This has proved to be a huge challenge due to the wide range of available nanomaterials and their unpredictable behaviour in different environments. Furthermore, the enormous number of experimental variables make comparisons difficult. Concentration is one of these variables and can vary greatly depending on the aim of the study. When analysing the protein corona, concentrations are often higher than in cell assays. Using a combination of complementary techniques, we have characterised 20 nm gold nanoparticles in a concentration level commonly used in cell studies. We compare their behaviour in a commonly used, protein rich medium and one protein poor medium over 24 hours. Under these conditions, the NPs were stable in protein rich environment but underwent gradual aggregation in protein poor medium. We characterise the biomolecular corona in both media. In protein poor medium, we can describe the often overlooked aggregation. The aggregates' morphology is confirmed by cryo-TEM. Finally, in the protein poor medium, by infrared spectroscopy, we have identified the amino acid arginine in the biomolecular corona which drives the aggregation. |
| doi_str_mv | 10.1371/journal.pone.0218211 |
| format | Article |
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Assessing their biological impact has been done by analysis of their adsorbed protein corona, in vitro cell assays, and larger scale ecotoxicological studies. This has proved to be a huge challenge due to the wide range of available nanomaterials and their unpredictable behaviour in different environments. Furthermore, the enormous number of experimental variables make comparisons difficult. Concentration is one of these variables and can vary greatly depending on the aim of the study. When analysing the protein corona, concentrations are often higher than in cell assays. Using a combination of complementary techniques, we have characterised 20 nm gold nanoparticles in a concentration level commonly used in cell studies. We compare their behaviour in a commonly used, protein rich medium and one protein poor medium over 24 hours. Under these conditions, the NPs were stable in protein rich environment but underwent gradual aggregation in protein poor medium. We characterise the biomolecular corona in both media. In protein poor medium, we can describe the often overlooked aggregation. The aggregates' morphology is confirmed by cryo-TEM. Finally, in the protein poor medium, by infrared spectroscopy, we have identified the amino acid arginine in the biomolecular corona which drives the aggregation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0218211</identifier><identifier>PMID: 31199838</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agglomeration ; Amino acids ; Analysis ; Arginine ; Biochemistry ; Biochemistry and Molecular Biology ; Biofysiks ; Biokemi och molekylärbiologi ; Biologi ; Biological Sciences ; Biology ; Biology and Life Sciences ; Biomedical Sciences ; Biomedicinsk vetenskap ; Biophysics ; Cell culture ; Chemical properties ; Corona ; Cosmetics ; Culture media ; Culture Media - chemistry ; Electric properties ; Electron microscopy ; Engineering and Technology ; Environmental toxicology ; Fractals ; Gold ; Gold - chemistry ; Gold compounds ; High technology ; Infrared spectroscopy ; Media (culture) ; Medical and Health Sciences ; Medical Biotechnology ; Medicin och hälsovetenskap ; Medicinsk bioteknologi ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - ultrastructure ; Morphology ; Nanomaterials ; Nanoparticles ; Nanostructured materials ; Nanotechnology ; Natural Sciences ; Naturvetenskap ; Optical properties ; Physical Sciences ; Protein Corona - chemistry ; Proteins ; Research and Analysis Methods ; Sedimentation & deposition ; Silver ; Spectroscopy ; Spectrum analysis ; Technology</subject><ispartof>PloS one, 2019-06, Vol.14 (6), p.e0218211</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Gunnarsson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Assessing their biological impact has been done by analysis of their adsorbed protein corona, in vitro cell assays, and larger scale ecotoxicological studies. This has proved to be a huge challenge due to the wide range of available nanomaterials and their unpredictable behaviour in different environments. Furthermore, the enormous number of experimental variables make comparisons difficult. Concentration is one of these variables and can vary greatly depending on the aim of the study. When analysing the protein corona, concentrations are often higher than in cell assays. Using a combination of complementary techniques, we have characterised 20 nm gold nanoparticles in a concentration level commonly used in cell studies. We compare their behaviour in a commonly used, protein rich medium and one protein poor medium over 24 hours. Under these conditions, the NPs were stable in protein rich environment but underwent gradual aggregation in protein poor medium. We characterise the biomolecular corona in both media. In protein poor medium, we can describe the often overlooked aggregation. The aggregates' morphology is confirmed by cryo-TEM. Finally, in the protein poor medium, by infrared spectroscopy, we have identified the amino acid arginine in the biomolecular corona which drives the aggregation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31199838</pmid><doi>10.1371/journal.pone.0218211</doi><tpages>e0218211</tpages><orcidid>https://orcid.org/0000-0003-2255-8446</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-06, Vol.14 (6), p.e0218211 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2240300685 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; SWEPUB Freely available online; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Agglomeration Amino acids Analysis Arginine Biochemistry Biochemistry and Molecular Biology Biofysiks Biokemi och molekylärbiologi Biologi Biological Sciences Biology Biology and Life Sciences Biomedical Sciences Biomedicinsk vetenskap Biophysics Cell culture Chemical properties Corona Cosmetics Culture media Culture Media - chemistry Electric properties Electron microscopy Engineering and Technology Environmental toxicology Fractals Gold Gold - chemistry Gold compounds High technology Infrared spectroscopy Media (culture) Medical and Health Sciences Medical Biotechnology Medicin och hälsovetenskap Medicinsk bioteknologi Metal Nanoparticles - chemistry Metal Nanoparticles - ultrastructure Morphology Nanomaterials Nanoparticles Nanostructured materials Nanotechnology Natural Sciences Naturvetenskap Optical properties Physical Sciences Protein Corona - chemistry Proteins Research and Analysis Methods Sedimentation & deposition Silver Spectroscopy Spectrum analysis Technology |
| title | Analysis of complexes formed by small gold nanoparticles in low concentration in cell culture media |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T15%3A41%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analysis%20of%20complexes%20formed%20by%20small%20gold%20nanoparticles%20in%20low%20concentration%20in%20cell%20culture%20media&rft.jtitle=PloS%20one&rft.au=Gunnarsson,%20Stef%C3%A1n%20B&rft.date=2019-06-14&rft.volume=14&rft.issue=6&rft.spage=e0218211&rft.pages=e0218211-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0218211&rft_dat=%3Cgale_plos_%3EA589072963%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2240300685&rft_id=info:pmid/31199838&rft_galeid=A589072963&rft_doaj_id=oai_doaj_org_article_3bea796f2e2c4d579562e1cdceec744f&rfr_iscdi=true |