Emergence of an Australian-like pstS-null vancomycin resistant Enterococcus faecium clone in Scotland
Multi-locus sequencing typing (MLST) is widely used to monitor the phylogeny of microbial outbreaks. However, several strains of vancomycin-resistant Enterococcus faecium (VREfm) with a missing MLST locus (pstS) have recently emerged in Australia, with a few cases also reported in England. Here, we...
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| Veröffentlicht in: | PloS one 2019-06, Vol.14 (6), p.e0218185-e0218185 |
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| creator | Lemonidis, Kimon Salih, Talal S Dancer, Stephanie J Hunter, Iain S Tucker, Nicholas P |
| description | Multi-locus sequencing typing (MLST) is widely used to monitor the phylogeny of microbial outbreaks. However, several strains of vancomycin-resistant Enterococcus faecium (VREfm) with a missing MLST locus (pstS) have recently emerged in Australia, with a few cases also reported in England. Here, we identified similarly distinct strains circulating in two neighbouring hospitals in Scotland. Whole genome sequencing of five VREfm strains isolated from these hospitals identified four pstS-null strains in both hospitals, while the fifth was multi-locus sequence type (ST) 262, which is the first documented in the UK. All five Scottish isolates had an insertion in the tetM gene, which is associated with increased susceptibility to tetracyclines, providing no other tetracycline-resistant gene is present. Such an insertion, which encompasses a dfrG gene and two currently uncharacterised genes, was additionally identified in all tested vanA-type pstS-null VREfm strains (5 English and 68 Australian). Phylogenetic comparison with other VREfm genomes indicates that the four pstS-null Scottish isolates sequenced in this study are more closely related to pstS-null strains from Australia rather than the English pstS-null isolates. Given how rapidly such pstS-null strains have expanded in Australia, the emergence of this clone in Scotland raises concerns for a potential outbreak. |
| doi_str_mv | 10.1371/journal.pone.0218185 |
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However, several strains of vancomycin-resistant Enterococcus faecium (VREfm) with a missing MLST locus (pstS) have recently emerged in Australia, with a few cases also reported in England. Here, we identified similarly distinct strains circulating in two neighbouring hospitals in Scotland. Whole genome sequencing of five VREfm strains isolated from these hospitals identified four pstS-null strains in both hospitals, while the fifth was multi-locus sequence type (ST) 262, which is the first documented in the UK. All five Scottish isolates had an insertion in the tetM gene, which is associated with increased susceptibility to tetracyclines, providing no other tetracycline-resistant gene is present. Such an insertion, which encompasses a dfrG gene and two currently uncharacterised genes, was additionally identified in all tested vanA-type pstS-null VREfm strains (5 English and 68 Australian). Phylogenetic comparison with other VREfm genomes indicates that the four pstS-null Scottish isolates sequenced in this study are more closely related to pstS-null strains from Australia rather than the English pstS-null isolates. Given how rapidly such pstS-null strains have expanded in Australia, the emergence of this clone in Scotland raises concerns for a potential outbreak.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0218185</identifier><identifier>PMID: 31194809</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Anti-Bacterial Agents - therapeutic use ; Antibacterial agents ; Antibiotic resistance ; Antibiotics ; Antimicrobial agents ; Australia ; Biology and Life Sciences ; Computer and Information Sciences ; Cross Infection - drug therapy ; Cross Infection - genetics ; Cross Infection - microbiology ; Dalfopristin ; Deoxyribonucleic acid ; Disease Outbreaks ; DNA ; DNA sequencing ; Drug resistance ; Emergence ; England ; Enterococcus ; Enterococcus faecium ; Enterococcus faecium - drug effects ; Enterococcus faecium - genetics ; Gene sequencing ; Genes ; Genetic aspects ; Genomes ; Genomics ; Genotype ; Gram-positive bacteria ; Gram-Positive Bacterial Infections - drug therapy ; Gram-Positive Bacterial Infections - genetics ; Gram-Positive Bacterial Infections - microbiology ; Hospitals ; Humans ; Infections ; Insertion ; Loci ; Medicine and Health Sciences ; Metronidazole ; Microbial drug resistance ; Microbial Sensitivity Tests - methods ; Microorganisms ; Multilocus sequence typing ; Multilocus Sequence Typing - methods ; Outbreaks ; Oxytetracycline ; Pharmacy ; Phylogeny ; Physiological aspects ; Quinupristin ; Research and Analysis Methods ; Scotland ; Semiconductors ; TetM gene ; Tetracyclines ; Tigecycline ; Vancomycin ; Vancomycin - therapeutic use ; Vancomycin-Resistant Enterococci - genetics</subject><ispartof>PloS one, 2019-06, Vol.14 (6), p.e0218185-e0218185</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Lemonidis et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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However, several strains of vancomycin-resistant Enterococcus faecium (VREfm) with a missing MLST locus (pstS) have recently emerged in Australia, with a few cases also reported in England. Here, we identified similarly distinct strains circulating in two neighbouring hospitals in Scotland. Whole genome sequencing of five VREfm strains isolated from these hospitals identified four pstS-null strains in both hospitals, while the fifth was multi-locus sequence type (ST) 262, which is the first documented in the UK. All five Scottish isolates had an insertion in the tetM gene, which is associated with increased susceptibility to tetracyclines, providing no other tetracycline-resistant gene is present. Such an insertion, which encompasses a dfrG gene and two currently uncharacterised genes, was additionally identified in all tested vanA-type pstS-null VREfm strains (5 English and 68 Australian). Phylogenetic comparison with other VREfm genomes indicates that the four pstS-null Scottish isolates sequenced in this study are more closely related to pstS-null strains from Australia rather than the English pstS-null isolates. Given how rapidly such pstS-null strains have expanded in Australia, the emergence of this clone in Scotland raises concerns for a potential outbreak.</description><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Antibacterial agents</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Australia</subject><subject>Biology and Life Sciences</subject><subject>Computer and Information Sciences</subject><subject>Cross Infection - drug therapy</subject><subject>Cross Infection - genetics</subject><subject>Cross Infection - microbiology</subject><subject>Dalfopristin</subject><subject>Deoxyribonucleic acid</subject><subject>Disease Outbreaks</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>Drug resistance</subject><subject>Emergence</subject><subject>England</subject><subject>Enterococcus</subject><subject>Enterococcus faecium</subject><subject>Enterococcus faecium - drug effects</subject><subject>Enterococcus faecium - 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However, several strains of vancomycin-resistant Enterococcus faecium (VREfm) with a missing MLST locus (pstS) have recently emerged in Australia, with a few cases also reported in England. Here, we identified similarly distinct strains circulating in two neighbouring hospitals in Scotland. Whole genome sequencing of five VREfm strains isolated from these hospitals identified four pstS-null strains in both hospitals, while the fifth was multi-locus sequence type (ST) 262, which is the first documented in the UK. All five Scottish isolates had an insertion in the tetM gene, which is associated with increased susceptibility to tetracyclines, providing no other tetracycline-resistant gene is present. Such an insertion, which encompasses a dfrG gene and two currently uncharacterised genes, was additionally identified in all tested vanA-type pstS-null VREfm strains (5 English and 68 Australian). Phylogenetic comparison with other VREfm genomes indicates that the four pstS-null Scottish isolates sequenced in this study are more closely related to pstS-null strains from Australia rather than the English pstS-null isolates. Given how rapidly such pstS-null strains have expanded in Australia, the emergence of this clone in Scotland raises concerns for a potential outbreak.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31194809</pmid><doi>10.1371/journal.pone.0218185</doi><tpages>e0218185</tpages><orcidid>https://orcid.org/0000-0002-8777-2614</orcidid><orcidid>https://orcid.org/0000-0003-1332-6002</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2019-06, Vol.14 (6), p.e0218185-e0218185 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2239658322 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Anti-Bacterial Agents - therapeutic use Antibacterial agents Antibiotic resistance Antibiotics Antimicrobial agents Australia Biology and Life Sciences Computer and Information Sciences Cross Infection - drug therapy Cross Infection - genetics Cross Infection - microbiology Dalfopristin Deoxyribonucleic acid Disease Outbreaks DNA DNA sequencing Drug resistance Emergence England Enterococcus Enterococcus faecium Enterococcus faecium - drug effects Enterococcus faecium - genetics Gene sequencing Genes Genetic aspects Genomes Genomics Genotype Gram-positive bacteria Gram-Positive Bacterial Infections - drug therapy Gram-Positive Bacterial Infections - genetics Gram-Positive Bacterial Infections - microbiology Hospitals Humans Infections Insertion Loci Medicine and Health Sciences Metronidazole Microbial drug resistance Microbial Sensitivity Tests - methods Microorganisms Multilocus sequence typing Multilocus Sequence Typing - methods Outbreaks Oxytetracycline Pharmacy Phylogeny Physiological aspects Quinupristin Research and Analysis Methods Scotland Semiconductors TetM gene Tetracyclines Tigecycline Vancomycin Vancomycin - therapeutic use Vancomycin-Resistant Enterococci - genetics |
| title | Emergence of an Australian-like pstS-null vancomycin resistant Enterococcus faecium clone in Scotland |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T15%3A42%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Emergence%20of%20an%20Australian-like%20pstS-null%20vancomycin%20resistant%20Enterococcus%20faecium%20clone%20in%20Scotland&rft.jtitle=PloS%20one&rft.au=Lemonidis,%20Kimon&rft.date=2019-06-13&rft.volume=14&rft.issue=6&rft.spage=e0218185&rft.epage=e0218185&rft.pages=e0218185-e0218185&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0218185&rft_dat=%3Cgale_plos_%3EA588924207%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2239658322&rft_id=info:pmid/31194809&rft_galeid=A588924207&rft_doaj_id=oai_doaj_org_article_b8350ff58f234db0be8f984f0db37e3e&rfr_iscdi=true |