Systemically transplanted mesenchymal stem cells induce vascular-like structure formation in a rat model of vaginal injury
The beneficial effect of mesenchymal stem cells (MSCs) on wound healing is mostly attributed to a trophic effect that promotes angiogenesis. Whether MSCs can contribute to the formation of new blood vessels by direct differentiation is still controversial. Pelvic floor dysfunction (PFD) is a group o...
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| creator | Ben Menachem-Zidon, Ofra Gropp, Michal Ben Shushan, Etti Reubinoff, Benjamin Shveiky, David |
| description | The beneficial effect of mesenchymal stem cells (MSCs) on wound healing is mostly attributed to a trophic effect that promotes angiogenesis. Whether MSCs can contribute to the formation of new blood vessels by direct differentiation is still controversial. Pelvic floor dysfunction (PFD) is a group of disorders that negatively affect the quality of women's lives. Traditional vaginal surgical repair provides disappointing anatomical outcome. Stem cell transplantation may be used to supplement surgery and improve its outcome. Here we aimed to examine the engraftment, survival, differentiation and angiogenic effect of transplanted MSCs in a vaginal injury rat model. MSCs were obtained from the bone marrow of Sprague Drawley (SD) rats, expanded and characterized in vitro. The MSCs expressed CD90 and CD29, did not express CD45, CD34, CD11b and CD31 and could differentiate into osteogenic, chondrogenic and adipogenic lineages. Cells were labeled with either PKH-26 or GFP and transplanted systemically or locally to female SD rats, just after a standardized vaginal incision was made. Engraftment after local transplantation was less efficient at all-time points compared to systemic administration. In the systemically transplanted animal group, MSCs migrated to the injury site and were present in the healed vagina for at least 30 days. Both systemic and local MSCs transplantation promoted host angiogenesis. Systemically transplanted MSCs created new vascular-like structures by direct differentiation into endothelium. These findings pave the way to further studies of the potential role of MSCs transplantation in improving surgical outcome in women with PFD. |
| doi_str_mv | 10.1371/journal.pone.0218081 |
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Whether MSCs can contribute to the formation of new blood vessels by direct differentiation is still controversial. Pelvic floor dysfunction (PFD) is a group of disorders that negatively affect the quality of women's lives. Traditional vaginal surgical repair provides disappointing anatomical outcome. Stem cell transplantation may be used to supplement surgery and improve its outcome. Here we aimed to examine the engraftment, survival, differentiation and angiogenic effect of transplanted MSCs in a vaginal injury rat model. MSCs were obtained from the bone marrow of Sprague Drawley (SD) rats, expanded and characterized in vitro. The MSCs expressed CD90 and CD29, did not express CD45, CD34, CD11b and CD31 and could differentiate into osteogenic, chondrogenic and adipogenic lineages. Cells were labeled with either PKH-26 or GFP and transplanted systemically or locally to female SD rats, just after a standardized vaginal incision was made. Engraftment after local transplantation was less efficient at all-time points compared to systemic administration. In the systemically transplanted animal group, MSCs migrated to the injury site and were present in the healed vagina for at least 30 days. Both systemic and local MSCs transplantation promoted host angiogenesis. Systemically transplanted MSCs created new vascular-like structures by direct differentiation into endothelium. These findings pave the way to further studies of the potential role of MSCs transplantation in improving surgical outcome in women with PFD.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0218081</identifier><identifier>PMID: 31194823</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Angiogenesis ; Animals ; Biocompatibility ; Biology and Life Sciences ; Biomedical materials ; Blood vessels ; Blood Vessels - growth & development ; Bone marrow ; Bone marrow transplantation ; Care and treatment ; CD11b antigen ; CD29 antigen ; CD34 antigen ; CD45 antigen ; CD90 antigen ; Cell Differentiation ; Differentiation ; Disease Models, Animal ; EDTA ; Endothelium ; Endothelium, Vascular - cytology ; Female ; Gene therapy ; Gynecology ; Hospitals ; Injuries ; Medical research ; Medicine and Health Sciences ; Mesenchymal Stem Cell Transplantation ; Mesenchymal stem cells ; Mesenchymal Stem Cells - cytology ; Mesenchyme ; Neovascularization ; Obstetrics ; Patient outcomes ; Pelvic Floor Disorders - therapy ; Rats ; Rats, Sprague-Dawley ; Research and Analysis Methods ; Stem cell transplantation ; Stem cells ; Studies ; Surgery ; Transplantation ; Vagina ; Vagina - injuries ; Wound care ; Wound healing</subject><ispartof>PloS one, 2019-06, Vol.14 (6), p.e0218081</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Ben Menachem- Zidon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Whether MSCs can contribute to the formation of new blood vessels by direct differentiation is still controversial. Pelvic floor dysfunction (PFD) is a group of disorders that negatively affect the quality of women's lives. Traditional vaginal surgical repair provides disappointing anatomical outcome. Stem cell transplantation may be used to supplement surgery and improve its outcome. Here we aimed to examine the engraftment, survival, differentiation and angiogenic effect of transplanted MSCs in a vaginal injury rat model. MSCs were obtained from the bone marrow of Sprague Drawley (SD) rats, expanded and characterized in vitro. The MSCs expressed CD90 and CD29, did not express CD45, CD34, CD11b and CD31 and could differentiate into osteogenic, chondrogenic and adipogenic lineages. Cells were labeled with either PKH-26 or GFP and transplanted systemically or locally to female SD rats, just after a standardized vaginal incision was made. 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These findings pave the way to further studies of the potential role of MSCs transplantation in improving surgical outcome in women with PFD.</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biology and Life Sciences</subject><subject>Biomedical materials</subject><subject>Blood vessels</subject><subject>Blood Vessels - growth & development</subject><subject>Bone marrow</subject><subject>Bone marrow transplantation</subject><subject>Care and treatment</subject><subject>CD11b antigen</subject><subject>CD29 antigen</subject><subject>CD34 antigen</subject><subject>CD45 antigen</subject><subject>CD90 antigen</subject><subject>Cell Differentiation</subject><subject>Differentiation</subject><subject>Disease Models, Animal</subject><subject>EDTA</subject><subject>Endothelium</subject><subject>Endothelium, Vascular - cytology</subject><subject>Female</subject><subject>Gene therapy</subject><subject>Gynecology</subject><subject>Hospitals</subject><subject>Injuries</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - 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Whether MSCs can contribute to the formation of new blood vessels by direct differentiation is still controversial. Pelvic floor dysfunction (PFD) is a group of disorders that negatively affect the quality of women's lives. Traditional vaginal surgical repair provides disappointing anatomical outcome. Stem cell transplantation may be used to supplement surgery and improve its outcome. Here we aimed to examine the engraftment, survival, differentiation and angiogenic effect of transplanted MSCs in a vaginal injury rat model. MSCs were obtained from the bone marrow of Sprague Drawley (SD) rats, expanded and characterized in vitro. The MSCs expressed CD90 and CD29, did not express CD45, CD34, CD11b and CD31 and could differentiate into osteogenic, chondrogenic and adipogenic lineages. Cells were labeled with either PKH-26 or GFP and transplanted systemically or locally to female SD rats, just after a standardized vaginal incision was made. Engraftment after local transplantation was less efficient at all-time points compared to systemic administration. In the systemically transplanted animal group, MSCs migrated to the injury site and were present in the healed vagina for at least 30 days. Both systemic and local MSCs transplantation promoted host angiogenesis. Systemically transplanted MSCs created new vascular-like structures by direct differentiation into endothelium. These findings pave the way to further studies of the potential role of MSCs transplantation in improving surgical outcome in women with PFD.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31194823</pmid><doi>10.1371/journal.pone.0218081</doi><tpages>e0218081</tpages><orcidid>https://orcid.org/0000-0003-0353-1299</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Angiogenesis Animals Biocompatibility Biology and Life Sciences Biomedical materials Blood vessels Blood Vessels - growth & development Bone marrow Bone marrow transplantation Care and treatment CD11b antigen CD29 antigen CD34 antigen CD45 antigen CD90 antigen Cell Differentiation Differentiation Disease Models, Animal EDTA Endothelium Endothelium, Vascular - cytology Female Gene therapy Gynecology Hospitals Injuries Medical research Medicine and Health Sciences Mesenchymal Stem Cell Transplantation Mesenchymal stem cells Mesenchymal Stem Cells - cytology Mesenchyme Neovascularization Obstetrics Patient outcomes Pelvic Floor Disorders - therapy Rats Rats, Sprague-Dawley Research and Analysis Methods Stem cell transplantation Stem cells Studies Surgery Transplantation Vagina Vagina - injuries Wound care Wound healing |
| title | Systemically transplanted mesenchymal stem cells induce vascular-like structure formation in a rat model of vaginal injury |
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