Assessment of genome stability in various breeds of cattle
Chromosomal instability is a type of genome instability involving changes in genetic information at the chromosomal level. The basic tests used to identify this form of instability are sister chromatid exchange (SCE) tests and identification of fragile sites (FS). SCE is the process by which sister...
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| description | Chromosomal instability is a type of genome instability involving changes in genetic information at the chromosomal level. The basic tests used to identify this form of instability are sister chromatid exchange (SCE) tests and identification of fragile sites (FS). SCE is the process by which sister chromatids become fragmented as a result of DNA strand breakage and reassembly, followed by exchange of these fragments. FS can be observed in the form of breaks, gaps or constrictions on chromosomes, which often result from multiple nucleotide repeats in DNA that are difficult to replicate. The research material was the peripheral blood of ten breeds of cattle raised in Poland, including four native breeds covered by a genetic resources conservation programme, i.e. Polish Red, Polish Red-and-White, White-Backed, and Polish Black-and-White, as well as Polish Holstein-Friesian, Simmental, Montbéliarde, Jersey, Limousine and Danish Red. Two tests were performed on chromosomes obtained from in vitro cultures: SCE and FS. The average frequency of SCE was 5.08 ± 1.31, while the incidence of FS was 3.45 ± 0.94. Differences in the incidence of SCE and FS were observed between breeds. The least damage was observed in the Polish Red and White-Backed breeds, and the most in Polish Holstein-Friesians. The most damage was observed in the interstitial part of the chromosomes. Age was shown to significantly affect the incidence of SCE and FS. Younger cows showed less damage than older ones (SCE: 4.84 ± 1.25; 5.34 ± 1.24; FS: 3.10 ± 0.88, 3.80 ± 0.92). |
| doi_str_mv | 10.1371/journal.pone.0217799 |
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The basic tests used to identify this form of instability are sister chromatid exchange (SCE) tests and identification of fragile sites (FS). SCE is the process by which sister chromatids become fragmented as a result of DNA strand breakage and reassembly, followed by exchange of these fragments. FS can be observed in the form of breaks, gaps or constrictions on chromosomes, which often result from multiple nucleotide repeats in DNA that are difficult to replicate. The research material was the peripheral blood of ten breeds of cattle raised in Poland, including four native breeds covered by a genetic resources conservation programme, i.e. Polish Red, Polish Red-and-White, White-Backed, and Polish Black-and-White, as well as Polish Holstein-Friesian, Simmental, Montbéliarde, Jersey, Limousine and Danish Red. Two tests were performed on chromosomes obtained from in vitro cultures: SCE and FS. The average frequency of SCE was 5.08 ± 1.31, while the incidence of FS was 3.45 ± 0.94. Differences in the incidence of SCE and FS were observed between breeds. The least damage was observed in the Polish Red and White-Backed breeds, and the most in Polish Holstein-Friesians. The most damage was observed in the interstitial part of the chromosomes. Age was shown to significantly affect the incidence of SCE and FS. Younger cows showed less damage than older ones (SCE: 4.84 ± 1.25; 5.34 ± 1.24; FS: 3.10 ± 0.88, 3.80 ± 0.92).</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0217799</identifier><identifier>PMID: 31163060</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Beef cattle ; Bioengineering ; Biology and Life Sciences ; Bovidae ; Breakage ; Breeding ; Breeding of animals ; Cattle ; Cattle - genetics ; Cattle breeding ; Cell cycle ; Cell division ; Chromatids ; Chromosome abnormalities ; Chromosome Fragile Sites - genetics ; Chromosomes ; Chromosomes, Mammalian - genetics ; Computer and Information Sciences ; Conservation ; Damage ; Deoxyribonucleic acid ; Diagnosis ; Dioxins ; DNA ; DNA damage ; Exchanging ; Fragile sites ; Genetic aspects ; Genetic research ; Genetic resources ; Genomes ; Genomic Instability ; Genomics ; Health aspects ; Humanities ; Identification ; Incidence ; Instability ; Laboratory animals ; Metaphase - genetics ; Peripheral blood ; Resource conservation ; Sheep ; Sister chromatid exchange ; Sister Chromatid Exchange - genetics ; Sister chromatids ; Social Sciences ; Stability ; Stability analysis</subject><ispartof>PloS one, 2019-06, Vol.14 (6), p.e0217799</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Wójcik, Szostek. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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The basic tests used to identify this form of instability are sister chromatid exchange (SCE) tests and identification of fragile sites (FS). SCE is the process by which sister chromatids become fragmented as a result of DNA strand breakage and reassembly, followed by exchange of these fragments. FS can be observed in the form of breaks, gaps or constrictions on chromosomes, which often result from multiple nucleotide repeats in DNA that are difficult to replicate. The research material was the peripheral blood of ten breeds of cattle raised in Poland, including four native breeds covered by a genetic resources conservation programme, i.e. Polish Red, Polish Red-and-White, White-Backed, and Polish Black-and-White, as well as Polish Holstein-Friesian, Simmental, Montbéliarde, Jersey, Limousine and Danish Red. Two tests were performed on chromosomes obtained from in vitro cultures: SCE and FS. The average frequency of SCE was 5.08 ± 1.31, while the incidence of FS was 3.45 ± 0.94. Differences in the incidence of SCE and FS were observed between breeds. The least damage was observed in the Polish Red and White-Backed breeds, and the most in Polish Holstein-Friesians. The most damage was observed in the interstitial part of the chromosomes. Age was shown to significantly affect the incidence of SCE and FS. Younger cows showed less damage than older ones (SCE: 4.84 ± 1.25; 5.34 ± 1.24; FS: 3.10 ± 0.88, 3.80 ± 0.92).</description><subject>Animals</subject><subject>Beef cattle</subject><subject>Bioengineering</subject><subject>Biology and Life Sciences</subject><subject>Bovidae</subject><subject>Breakage</subject><subject>Breeding</subject><subject>Breeding of animals</subject><subject>Cattle</subject><subject>Cattle - genetics</subject><subject>Cattle breeding</subject><subject>Cell cycle</subject><subject>Cell division</subject><subject>Chromatids</subject><subject>Chromosome abnormalities</subject><subject>Chromosome Fragile Sites - genetics</subject><subject>Chromosomes</subject><subject>Chromosomes, Mammalian - genetics</subject><subject>Computer and Information Sciences</subject><subject>Conservation</subject><subject>Damage</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>Dioxins</subject><subject>DNA</subject><subject>DNA damage</subject><subject>Exchanging</subject><subject>Fragile sites</subject><subject>Genetic aspects</subject><subject>Genetic research</subject><subject>Genetic resources</subject><subject>Genomes</subject><subject>Genomic Instability</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Humanities</subject><subject>Identification</subject><subject>Incidence</subject><subject>Instability</subject><subject>Laboratory animals</subject><subject>Metaphase - genetics</subject><subject>Peripheral blood</subject><subject>Resource conservation</subject><subject>Sheep</subject><subject>Sister chromatid exchange</subject><subject>Sister Chromatid Exchange - genetics</subject><subject>Sister chromatids</subject><subject>Social Sciences</subject><subject>Stability</subject><subject>Stability analysis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7jr6D0QLgujFjPlO6oUwLH4MLCz4dRvS9HQmQ9uMTbq4_97U6S5T2QvJRcLJc96Tc_Jm2XOMVphK_G7vh74zzergO1ghgqUsigfZOS4oWQqC6MOT81n2JIQ9QpwqIR5nZxRjQZFA59n7dQgQQgtdzH2db6HzLeQhmtI1Lt7krsuvTe_8EPKyB6jCSFkTYwNPs0e1aQI8m_ZF9uPTx-8XX5aXV583F-vLpRUFicuKUlUgsBUGXhJUlJQAQbJQEnFATEpFuFDWKiSYZMB4ZQUuQBheqKq2nC6yl0fdQ-ODntoOmhDKEUMFxYnYHInKm70-9K41_Y32xum_Ad9vtemjsw1ogUorVMlrWmLGJBioKFSGW2GRNLROWh-makPZQmXTYHrTzETnN53b6a2_1oIzRYVMAm8mgd7_GiBE3bpgoWlMB2mM6d2MKEwIH9FX_6D3dzdRW5MacF3tU107iuo1V1JSJhO9yFb3UGlV0DqbPFK7FJ8lvJ0lJCbC77g1Qwh68-3r_7NXP-fs6xN2B6aJu-CbITrfhTnIjqDtfQg91HdDxkiPFr-dhh4trieLp7QXpx90l3TrafoH50L0rQ</recordid><startdate>20190604</startdate><enddate>20190604</enddate><creator>Wójcik, Ewa</creator><creator>Szostek, Małgorzata</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-9446-7132</orcidid></search><sort><creationdate>20190604</creationdate><title>Assessment of genome stability in various breeds of cattle</title><author>Wójcik, Ewa ; Szostek, Małgorzata</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-d33890ecd1e5b209b32e20798705e047782568cc806474e45dc619e6a598dfc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Beef cattle</topic><topic>Bioengineering</topic><topic>Biology and Life Sciences</topic><topic>Bovidae</topic><topic>Breakage</topic><topic>Breeding</topic><topic>Breeding of animals</topic><topic>Cattle</topic><topic>Cattle - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wójcik, Ewa</au><au>Szostek, Małgorzata</au><au>Loor, Juan J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of genome stability in various breeds of cattle</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-06-04</date><risdate>2019</risdate><volume>14</volume><issue>6</issue><spage>e0217799</spage><pages>e0217799-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Chromosomal instability is a type of genome instability involving changes in genetic information at the chromosomal level. The basic tests used to identify this form of instability are sister chromatid exchange (SCE) tests and identification of fragile sites (FS). SCE is the process by which sister chromatids become fragmented as a result of DNA strand breakage and reassembly, followed by exchange of these fragments. FS can be observed in the form of breaks, gaps or constrictions on chromosomes, which often result from multiple nucleotide repeats in DNA that are difficult to replicate. The research material was the peripheral blood of ten breeds of cattle raised in Poland, including four native breeds covered by a genetic resources conservation programme, i.e. Polish Red, Polish Red-and-White, White-Backed, and Polish Black-and-White, as well as Polish Holstein-Friesian, Simmental, Montbéliarde, Jersey, Limousine and Danish Red. Two tests were performed on chromosomes obtained from in vitro cultures: SCE and FS. The average frequency of SCE was 5.08 ± 1.31, while the incidence of FS was 3.45 ± 0.94. Differences in the incidence of SCE and FS were observed between breeds. The least damage was observed in the Polish Red and White-Backed breeds, and the most in Polish Holstein-Friesians. The most damage was observed in the interstitial part of the chromosomes. Age was shown to significantly affect the incidence of SCE and FS. Younger cows showed less damage than older ones (SCE: 4.84 ± 1.25; 5.34 ± 1.24; FS: 3.10 ± 0.88, 3.80 ± 0.92).</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31163060</pmid><doi>10.1371/journal.pone.0217799</doi><tpages>e0217799</tpages><orcidid>https://orcid.org/0000-0002-9446-7132</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Beef cattle Bioengineering Biology and Life Sciences Bovidae Breakage Breeding Breeding of animals Cattle Cattle - genetics Cattle breeding Cell cycle Cell division Chromatids Chromosome abnormalities Chromosome Fragile Sites - genetics Chromosomes Chromosomes, Mammalian - genetics Computer and Information Sciences Conservation Damage Deoxyribonucleic acid Diagnosis Dioxins DNA DNA damage Exchanging Fragile sites Genetic aspects Genetic research Genetic resources Genomes Genomic Instability Genomics Health aspects Humanities Identification Incidence Instability Laboratory animals Metaphase - genetics Peripheral blood Resource conservation Sheep Sister chromatid exchange Sister Chromatid Exchange - genetics Sister chromatids Social Sciences Stability Stability analysis |
| title | Assessment of genome stability in various breeds of cattle |
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