A microfluidic based biosensor for rapid detection of Salmonella in food products
An impedance based microfluidic biosensor for simultaneous and rapid detection of Salmonella serotypes B and D in ready-to-eat (RTE) Turkey matrix has been presented. Detection of Salmonella at a concentration as low as 300 cells/ml with a total detection time of 1 hour has been achieved. The sensor...
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| Veröffentlicht in: | PloS one 2019-05, Vol.14 (5), p.e0216873-e0216873 |
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| creator | Liu, Jiayu Jasim, Ibrahem Shen, Zhenyu Zhao, Lu Dweik, Majed Zhang, Shuping Almasri, Mahmoud |
| description | An impedance based microfluidic biosensor for simultaneous and rapid detection of Salmonella serotypes B and D in ready-to-eat (RTE) Turkey matrix has been presented. Detection of Salmonella at a concentration as low as 300 cells/ml with a total detection time of 1 hour has been achieved. The sensor has two sensing regions, with each formed from one interdigitated electrode array (IDE array) consisting of 50 finger pairs. First, Salmonella antibody type B and D were prepared and delivered to the sensor to functionalize each sensing region without causing any cross contamination. Then the RTE Turkey samples spiked with Salmonella types B and D were introduced into the biosensor via the antigen inlet. The response signal resulted from the binding between Salmonella and its specific antibody demonstrated the sensor's ability to detect a single type of pathogen, and multiple pathogens simultaneously. In addition, the biosensor's selectivity was tested using non-specific binding of E. coli O157 and E. coli DH5 Alpha while the IDE array was coated with the Salmonella antibody. The results also showed the sensor is capable to differentiate low concentration of live Salmonella cells from high concentration of dead Salmonella cells, and high concentration of E. coli cells. A detailed study on antibody immobilization that includes antibody concentration, antibody coating time (0.5-3 hours) and use of cross-linker has been performed. The study showed that Salmonella antibody to Salmonella antigen is not a factor of antibody concentration after electrodes were saturated with antibody, while the optimal coating time was found to be 1.5 hours, and the use of cross-linker has improved the signal response by 45-60%. |
| doi_str_mv | 10.1371/journal.pone.0216873 |
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Detection of Salmonella at a concentration as low as 300 cells/ml with a total detection time of 1 hour has been achieved. The sensor has two sensing regions, with each formed from one interdigitated electrode array (IDE array) consisting of 50 finger pairs. First, Salmonella antibody type B and D were prepared and delivered to the sensor to functionalize each sensing region without causing any cross contamination. Then the RTE Turkey samples spiked with Salmonella types B and D were introduced into the biosensor via the antigen inlet. The response signal resulted from the binding between Salmonella and its specific antibody demonstrated the sensor's ability to detect a single type of pathogen, and multiple pathogens simultaneously. In addition, the biosensor's selectivity was tested using non-specific binding of E. coli O157 and E. coli DH5 Alpha while the IDE array was coated with the Salmonella antibody. The results also showed the sensor is capable to differentiate low concentration of live Salmonella cells from high concentration of dead Salmonella cells, and high concentration of E. coli cells. A detailed study on antibody immobilization that includes antibody concentration, antibody coating time (0.5-3 hours) and use of cross-linker has been performed. The study showed that Salmonella antibody to Salmonella antigen is not a factor of antibody concentration after electrodes were saturated with antibody, while the optimal coating time was found to be 1.5 hours, and the use of cross-linker has improved the signal response by 45-60%.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0216873</identifier><identifier>PMID: 31086396</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acoustics ; Antibodies ; Antibodies, Immobilized - chemistry ; Antigens ; Arrays ; Bacteria ; Binding ; Biology and Life Sciences ; Biosensing Techniques - economics ; Biosensing Techniques - instrumentation ; Biosensors ; Carbon ; Coated electrodes ; Coatings ; Computer engineering ; Contamination ; Convenience foods ; Crosslinking ; Detection equipment ; E coli ; Electrodes ; Engineering and Technology ; Equipment Design ; Food ; Food Analysis - economics ; Food Analysis - instrumentation ; Food contamination ; Food Contamination - analysis ; Food production ; Food products ; Food safety ; Identification and classification ; Immobilization ; Medical research ; Medicine and Health Sciences ; Microfluidic Analytical Techniques - economics ; Microfluidic Analytical Techniques - instrumentation ; Microfluidics ; Nanoparticles ; Pathogenic microorganisms ; Pathogens ; Physical Sciences ; Product recalls ; Properties ; Research and Analysis Methods ; Salmonella ; Salmonella - isolation & purification ; Salmonella food poisoning ; Selectivity ; Sensors ; Serotypes ; Time Factors ; Veterinary colleges ; Veterinary medicine</subject><ispartof>PloS one, 2019-05, Vol.14 (5), p.e0216873-e0216873</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Liu et al 2019 Liu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-defbcca2c12ea73e71ecc366c885fdc44cae99de7158361c2ce3f813009ecfe03</citedby><cites>FETCH-LOGICAL-c692t-defbcca2c12ea73e71ecc366c885fdc44cae99de7158361c2ce3f813009ecfe03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6516674/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6516674/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31086396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Jiayu</creatorcontrib><creatorcontrib>Jasim, Ibrahem</creatorcontrib><creatorcontrib>Shen, Zhenyu</creatorcontrib><creatorcontrib>Zhao, Lu</creatorcontrib><creatorcontrib>Dweik, Majed</creatorcontrib><creatorcontrib>Zhang, Shuping</creatorcontrib><creatorcontrib>Almasri, Mahmoud</creatorcontrib><title>A microfluidic based biosensor for rapid detection of Salmonella in food products</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>An impedance based microfluidic biosensor for simultaneous and rapid detection of Salmonella serotypes B and D in ready-to-eat (RTE) Turkey matrix has been presented. Detection of Salmonella at a concentration as low as 300 cells/ml with a total detection time of 1 hour has been achieved. The sensor has two sensing regions, with each formed from one interdigitated electrode array (IDE array) consisting of 50 finger pairs. First, Salmonella antibody type B and D were prepared and delivered to the sensor to functionalize each sensing region without causing any cross contamination. Then the RTE Turkey samples spiked with Salmonella types B and D were introduced into the biosensor via the antigen inlet. The response signal resulted from the binding between Salmonella and its specific antibody demonstrated the sensor's ability to detect a single type of pathogen, and multiple pathogens simultaneously. In addition, the biosensor's selectivity was tested using non-specific binding of E. coli O157 and E. coli DH5 Alpha while the IDE array was coated with the Salmonella antibody. The results also showed the sensor is capable to differentiate low concentration of live Salmonella cells from high concentration of dead Salmonella cells, and high concentration of E. coli cells. A detailed study on antibody immobilization that includes antibody concentration, antibody coating time (0.5-3 hours) and use of cross-linker has been performed. The study showed that Salmonella antibody to Salmonella antigen is not a factor of antibody concentration after electrodes were saturated with antibody, while the optimal coating time was found to be 1.5 hours, and the use of cross-linker has improved the signal response by 45-60%.</description><subject>Acoustics</subject><subject>Antibodies</subject><subject>Antibodies, Immobilized - chemistry</subject><subject>Antigens</subject><subject>Arrays</subject><subject>Bacteria</subject><subject>Binding</subject><subject>Biology and Life Sciences</subject><subject>Biosensing Techniques - economics</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensors</subject><subject>Carbon</subject><subject>Coated electrodes</subject><subject>Coatings</subject><subject>Computer engineering</subject><subject>Contamination</subject><subject>Convenience foods</subject><subject>Crosslinking</subject><subject>Detection equipment</subject><subject>E coli</subject><subject>Electrodes</subject><subject>Engineering and Technology</subject><subject>Equipment Design</subject><subject>Food</subject><subject>Food Analysis - economics</subject><subject>Food Analysis - instrumentation</subject><subject>Food contamination</subject><subject>Food Contamination - analysis</subject><subject>Food production</subject><subject>Food products</subject><subject>Food safety</subject><subject>Identification and classification</subject><subject>Immobilization</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Microfluidic Analytical Techniques - economics</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Microfluidics</subject><subject>Nanoparticles</subject><subject>Pathogenic microorganisms</subject><subject>Pathogens</subject><subject>Physical Sciences</subject><subject>Product recalls</subject><subject>Properties</subject><subject>Research and Analysis Methods</subject><subject>Salmonella</subject><subject>Salmonella - isolation & purification</subject><subject>Salmonella food poisoning</subject><subject>Selectivity</subject><subject>Sensors</subject><subject>Serotypes</subject><subject>Time Factors</subject><subject>Veterinary colleges</subject><subject>Veterinary medicine</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7of-A9GCsOjFjPlo0_RGGBY_BhYWXfU2pMnJTIZM001a0X9v6nSXqeyFlNBw8pz3JOe8WfYCoyWmFX6380NopVt2voUlIpjxij7KTnFNyYIRRB8f7U-ysxh3CJWUM_Y0O6EYcUZrdpp9WeV7q4I3brDaqryREXTeWB-hjT7kJq0gO6tzDT2o3vo29ya_kW6f6jonc9smyOu8C14Pqo_PsidGugjPp_959v3jh2-XnxdX15_Wl6urhWI16RcaTKOUJAoTkBWFCoNSlDHFeWm0Kgoloa51ipecMqyIAmo4pgjVoAwgep69Ouh2zkcxdSMKQkiZqJJViVgfCO3lTnTB7mX4Lby04m_Ah42QobfKgai5KZpS4wZrXYA0DSlRajLWlawbjmjSej9VG5o9aAVtH6Sbic5PWrsVG_9TsBIzVhVJ4M0kEPztALEXexvV2MEW_DDemxKEioKwhL7-B334dRO1kekBtjU-1VWjqFiVvCwwL-h47-UDVPo0pLmnERqb4rOEt7OExPTwq9_IIUaxvvn6_-z1jzl7ccRuQbp-G70bRkfFOVgcwOTKGAOY-yZjJEbn33VDjM4Xk_NT2svjAd0n3Vmd_gHB-f6w</recordid><startdate>20190514</startdate><enddate>20190514</enddate><creator>Liu, Jiayu</creator><creator>Jasim, Ibrahem</creator><creator>Shen, Zhenyu</creator><creator>Zhao, Lu</creator><creator>Dweik, Majed</creator><creator>Zhang, Shuping</creator><creator>Almasri, Mahmoud</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20190514</creationdate><title>A microfluidic based biosensor for rapid detection of Salmonella in food products</title><author>Liu, Jiayu ; Jasim, Ibrahem ; Shen, Zhenyu ; Zhao, Lu ; Dweik, Majed ; Zhang, Shuping ; Almasri, Mahmoud</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-defbcca2c12ea73e71ecc366c885fdc44cae99de7158361c2ce3f813009ecfe03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acoustics</topic><topic>Antibodies</topic><topic>Antibodies, Immobilized - chemistry</topic><topic>Antigens</topic><topic>Arrays</topic><topic>Bacteria</topic><topic>Binding</topic><topic>Biology and Life Sciences</topic><topic>Biosensing Techniques - economics</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensors</topic><topic>Carbon</topic><topic>Coated electrodes</topic><topic>Coatings</topic><topic>Computer engineering</topic><topic>Contamination</topic><topic>Convenience foods</topic><topic>Crosslinking</topic><topic>Detection equipment</topic><topic>E coli</topic><topic>Electrodes</topic><topic>Engineering and Technology</topic><topic>Equipment Design</topic><topic>Food</topic><topic>Food Analysis - economics</topic><topic>Food Analysis - instrumentation</topic><topic>Food contamination</topic><topic>Food Contamination - analysis</topic><topic>Food production</topic><topic>Food products</topic><topic>Food safety</topic><topic>Identification and classification</topic><topic>Immobilization</topic><topic>Medical research</topic><topic>Medicine and Health Sciences</topic><topic>Microfluidic Analytical Techniques - 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Detection of Salmonella at a concentration as low as 300 cells/ml with a total detection time of 1 hour has been achieved. The sensor has two sensing regions, with each formed from one interdigitated electrode array (IDE array) consisting of 50 finger pairs. First, Salmonella antibody type B and D were prepared and delivered to the sensor to functionalize each sensing region without causing any cross contamination. Then the RTE Turkey samples spiked with Salmonella types B and D were introduced into the biosensor via the antigen inlet. The response signal resulted from the binding between Salmonella and its specific antibody demonstrated the sensor's ability to detect a single type of pathogen, and multiple pathogens simultaneously. In addition, the biosensor's selectivity was tested using non-specific binding of E. coli O157 and E. coli DH5 Alpha while the IDE array was coated with the Salmonella antibody. The results also showed the sensor is capable to differentiate low concentration of live Salmonella cells from high concentration of dead Salmonella cells, and high concentration of E. coli cells. A detailed study on antibody immobilization that includes antibody concentration, antibody coating time (0.5-3 hours) and use of cross-linker has been performed. The study showed that Salmonella antibody to Salmonella antigen is not a factor of antibody concentration after electrodes were saturated with antibody, while the optimal coating time was found to be 1.5 hours, and the use of cross-linker has improved the signal response by 45-60%.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31086396</pmid><doi>10.1371/journal.pone.0216873</doi><tpages>e0216873</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-05, Vol.14 (5), p.e0216873-e0216873 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Acoustics Antibodies Antibodies, Immobilized - chemistry Antigens Arrays Bacteria Binding Biology and Life Sciences Biosensing Techniques - economics Biosensing Techniques - instrumentation Biosensors Carbon Coated electrodes Coatings Computer engineering Contamination Convenience foods Crosslinking Detection equipment E coli Electrodes Engineering and Technology Equipment Design Food Food Analysis - economics Food Analysis - instrumentation Food contamination Food Contamination - analysis Food production Food products Food safety Identification and classification Immobilization Medical research Medicine and Health Sciences Microfluidic Analytical Techniques - economics Microfluidic Analytical Techniques - instrumentation Microfluidics Nanoparticles Pathogenic microorganisms Pathogens Physical Sciences Product recalls Properties Research and Analysis Methods Salmonella Salmonella - isolation & purification Salmonella food poisoning Selectivity Sensors Serotypes Time Factors Veterinary colleges Veterinary medicine |
| title | A microfluidic based biosensor for rapid detection of Salmonella in food products |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T00%3A41%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20microfluidic%20based%20biosensor%20for%20rapid%20detection%20of%20Salmonella%20in%20food%20products&rft.jtitle=PloS%20one&rft.au=Liu,%20Jiayu&rft.date=2019-05-14&rft.volume=14&rft.issue=5&rft.spage=e0216873&rft.epage=e0216873&rft.pages=e0216873-e0216873&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0216873&rft_dat=%3Cgale_plos_%3EA585418433%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2225130567&rft_id=info:pmid/31086396&rft_galeid=A585418433&rft_doaj_id=oai_doaj_org_article_98f4b5d1b1dd4eafb2503711d7a9b803&rfr_iscdi=true |