Flow imaging microscopy as a novel tool for high-throughput evaluation of elastin-like polymer coacervates
Biological and bioinspired polymer microparticles have broad biomedical and industrial applications, including drug delivery, tissue engineering, surface modification, environmental remediation, imaging, and sensing. Full realization of the potential of biopolymer microparticles will require methods...
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| Veröffentlicht in: | PloS one 2019-05, Vol.14 (5), p.e0216406-e0216406 |
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| creator | Marvin, Laura Paiva, Wynter Gill, Nicole Morales, Marissa A Halpern, Jeffrey Mark Vesenka, James Balog, Eva Rose M |
| description | Biological and bioinspired polymer microparticles have broad biomedical and industrial applications, including drug delivery, tissue engineering, surface modification, environmental remediation, imaging, and sensing. Full realization of the potential of biopolymer microparticles will require methods for rigorous characterization of particle sizes, morphologies, and dynamics, so that researchers may correlate particle characteristics with synthesis methods and desired functions. Toward this end, we evaluated biopolymer microparticles using flow imaging microscopy. This technology is widely used in the biopharmaceutical industry but is not yet well-known among the materials community. Our polymer, a genetically engineered elastin-like polypeptide (ELP), self-assembles into micron-scale coacervates. We performed flow imaging of ELP coacervates using two different instruments, one with a lower size limit of approximately 2 microns, the other with a lower size limit of approximately 300 nanometers. We validated flow imaging results by comparison with dynamic light scattering and atomic force microscopy analyses. We explored the effects of various solvent conditions on ELP coacervate size, morphology, and behavior, such as the dispersion of single particles versus aggregates. We found that flow imaging is a superior tool for rapid and thorough particle analysis of ELP coacervates in solution. We anticipate that researchers studying many types of microscale protein or polymer assemblies will be interested in flow imaging as a tool for quantitative, solution-based characterization. |
| doi_str_mv | 10.1371/journal.pone.0216406 |
| format | Article |
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Full realization of the potential of biopolymer microparticles will require methods for rigorous characterization of particle sizes, morphologies, and dynamics, so that researchers may correlate particle characteristics with synthesis methods and desired functions. Toward this end, we evaluated biopolymer microparticles using flow imaging microscopy. This technology is widely used in the biopharmaceutical industry but is not yet well-known among the materials community. Our polymer, a genetically engineered elastin-like polypeptide (ELP), self-assembles into micron-scale coacervates. We performed flow imaging of ELP coacervates using two different instruments, one with a lower size limit of approximately 2 microns, the other with a lower size limit of approximately 300 nanometers. We validated flow imaging results by comparison with dynamic light scattering and atomic force microscopy analyses. We explored the effects of various solvent conditions on ELP coacervate size, morphology, and behavior, such as the dispersion of single particles versus aggregates. We found that flow imaging is a superior tool for rapid and thorough particle analysis of ELP coacervates in solution. We anticipate that researchers studying many types of microscale protein or polymer assemblies will be interested in flow imaging as a tool for quantitative, solution-based characterization.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0216406</identifier><identifier>PMID: 31071134</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Atomic force microscopy ; Biomedical engineering ; Biomedical materials ; Biopharmaceuticals ; Biopolymers ; Carbenicillin ; Chemical engineering ; Chemistry ; Drug delivery ; Drug Delivery Systems ; Drug Evaluation, Preclinical ; Elastin ; Elastin - chemistry ; Engineering and Technology ; Flow ; Genetic engineering ; Genetically modified organisms ; Imaging ; Industrial applications ; Industrial equipment ; Light scattering ; Methods ; Microparticles ; Microscopy ; Morphology ; Nanoparticles ; Particle analysis ; Pharmaceuticals ; Phase transitions ; Photon correlation spectroscopy ; Physical Sciences ; Physics ; Polymer industry ; Polymers ; Polypeptides ; Proteins ; Research and Analysis Methods ; Technology ; Tissue engineering</subject><ispartof>PloS one, 2019-05, Vol.14 (5), p.e0216406-e0216406</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Marvin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Full realization of the potential of biopolymer microparticles will require methods for rigorous characterization of particle sizes, morphologies, and dynamics, so that researchers may correlate particle characteristics with synthesis methods and desired functions. Toward this end, we evaluated biopolymer microparticles using flow imaging microscopy. This technology is widely used in the biopharmaceutical industry but is not yet well-known among the materials community. Our polymer, a genetically engineered elastin-like polypeptide (ELP), self-assembles into micron-scale coacervates. We performed flow imaging of ELP coacervates using two different instruments, one with a lower size limit of approximately 2 microns, the other with a lower size limit of approximately 300 nanometers. We validated flow imaging results by comparison with dynamic light scattering and atomic force microscopy analyses. 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We anticipate that researchers studying many types of microscale protein or polymer assemblies will be interested in flow imaging as a tool for quantitative, solution-based characterization.</description><subject>Atomic force microscopy</subject><subject>Biomedical engineering</subject><subject>Biomedical materials</subject><subject>Biopharmaceuticals</subject><subject>Biopolymers</subject><subject>Carbenicillin</subject><subject>Chemical engineering</subject><subject>Chemistry</subject><subject>Drug delivery</subject><subject>Drug Delivery Systems</subject><subject>Drug Evaluation, Preclinical</subject><subject>Elastin</subject><subject>Elastin - chemistry</subject><subject>Engineering and Technology</subject><subject>Flow</subject><subject>Genetic engineering</subject><subject>Genetically modified organisms</subject><subject>Imaging</subject><subject>Industrial applications</subject><subject>Industrial equipment</subject><subject>Light 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imaging microscopy as a novel tool for high-throughput evaluation of elastin-like polymer coacervates</title><author>Marvin, Laura ; Paiva, Wynter ; Gill, Nicole ; Morales, Marissa A ; Halpern, Jeffrey Mark ; Vesenka, James ; Balog, Eva Rose M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-79ab3b3a2ea598c158254a64445b2890fa5a05b094eb6b0d87ccb779abc52adc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Atomic force microscopy</topic><topic>Biomedical engineering</topic><topic>Biomedical materials</topic><topic>Biopharmaceuticals</topic><topic>Biopolymers</topic><topic>Carbenicillin</topic><topic>Chemical engineering</topic><topic>Chemistry</topic><topic>Drug delivery</topic><topic>Drug Delivery Systems</topic><topic>Drug Evaluation, Preclinical</topic><topic>Elastin</topic><topic>Elastin - chemistry</topic><topic>Engineering and 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one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marvin, Laura</au><au>Paiva, Wynter</au><au>Gill, Nicole</au><au>Morales, Marissa A</au><au>Halpern, Jeffrey Mark</au><au>Vesenka, James</au><au>Balog, Eva Rose M</au><au>Zelikin, Alexander N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow imaging microscopy as a novel tool for high-throughput evaluation of elastin-like polymer coacervates</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-05-09</date><risdate>2019</risdate><volume>14</volume><issue>5</issue><spage>e0216406</spage><epage>e0216406</epage><pages>e0216406-e0216406</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Biological and bioinspired polymer microparticles have broad biomedical and industrial applications, including drug delivery, tissue engineering, surface modification, environmental remediation, imaging, and sensing. Full realization of the potential of biopolymer microparticles will require methods for rigorous characterization of particle sizes, morphologies, and dynamics, so that researchers may correlate particle characteristics with synthesis methods and desired functions. Toward this end, we evaluated biopolymer microparticles using flow imaging microscopy. This technology is widely used in the biopharmaceutical industry but is not yet well-known among the materials community. Our polymer, a genetically engineered elastin-like polypeptide (ELP), self-assembles into micron-scale coacervates. We performed flow imaging of ELP coacervates using two different instruments, one with a lower size limit of approximately 2 microns, the other with a lower size limit of approximately 300 nanometers. We validated flow imaging results by comparison with dynamic light scattering and atomic force microscopy analyses. We explored the effects of various solvent conditions on ELP coacervate size, morphology, and behavior, such as the dispersion of single particles versus aggregates. We found that flow imaging is a superior tool for rapid and thorough particle analysis of ELP coacervates in solution. We anticipate that researchers studying many types of microscale protein or polymer assemblies will be interested in flow imaging as a tool for quantitative, solution-based characterization.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31071134</pmid><doi>10.1371/journal.pone.0216406</doi><tpages>e0216406</tpages><orcidid>https://orcid.org/0000-0003-0814-1986</orcidid><orcidid>https://orcid.org/0000-0002-6490-4071</orcidid><orcidid>https://orcid.org/0000-0001-6792-6914</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Atomic force microscopy Biomedical engineering Biomedical materials Biopharmaceuticals Biopolymers Carbenicillin Chemical engineering Chemistry Drug delivery Drug Delivery Systems Drug Evaluation, Preclinical Elastin Elastin - chemistry Engineering and Technology Flow Genetic engineering Genetically modified organisms Imaging Industrial applications Industrial equipment Light scattering Methods Microparticles Microscopy Morphology Nanoparticles Particle analysis Pharmaceuticals Phase transitions Photon correlation spectroscopy Physical Sciences Physics Polymer industry Polymers Polypeptides Proteins Research and Analysis Methods Technology Tissue engineering |
| title | Flow imaging microscopy as a novel tool for high-throughput evaluation of elastin-like polymer coacervates |
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