"Smart Eye Camera": An innovative technique to evaluate tear film breakup time in a murine dry eye disease model
Tear film breakup time (TFBUT) is an essential parameter used to diagnose dry eye disease (DED). However, a robust method for examining TFBUT in murine models has yet to be established. We invented an innovative device, namely, the "Smart Eye Camera", which addresses several problems assoc...
Gespeichert in:
| Veröffentlicht in: | PloS one 2019-05, Vol.14 (5), p.e0215130-e0215130 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0215130 |
|---|---|
| container_issue | 5 |
| container_start_page | e0215130 |
| container_title | PloS one |
| container_volume | 14 |
| creator | Shimizu, Eisuke Ogawa, Yoko Yazu, Hiroyuki Aketa, Naohiko Yang, Fan Yamane, Mio Sato, Yasunori Kawakami, Yutaka Tsubota, Kazuo |
| description | Tear film breakup time (TFBUT) is an essential parameter used to diagnose dry eye disease (DED). However, a robust method for examining TFBUT in murine models has yet to be established. We invented an innovative device, namely, the "Smart Eye Camera", which addresses several problems associated with existing methods and is capable of evaluating TFBUT in a murine DED model. We compared images taken by existing devices and the Smart Eye Camera in a graft-versus-host disease-related DED murine model. We observed that the quality of the images obtained by the Smart Eye Camera were sufficient for practical use. Moreover, this new technique could be used to obtain measurements for several consecutive ocular phenotypes in a variety of environments. Here, we demonstrate the effectiveness of our new invention in the examination of ocular phenotypes, including TFBUT in a murine model. We highlight the potential for future translational studies adopting the Smart Eye Camera in clinical settings. |
| doi_str_mv | 10.1371/journal.pone.0215130 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2222681027</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A584928769</galeid><doaj_id>oai_doaj_org_article_52aa8dd9f7534b0dba517e716d9a6868</doaj_id><sourcerecordid>A584928769</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-8b6078f8a90dd51b80014ef1562846f648d345f91145efded63afd084e607bf13</originalsourceid><addsrcrecordid>eNqNk19v0zAUxSMEYmPwDRBYQ0Lw0GLnj-PwgFRVAypNmsSAV-smvmk9Eruzk4p-exyaTQ3aA_FDLOd3ju1zc6PoJaNzluTsw43tnYFmvrUG5zRmGUvoo-iUFUk84zFNHh_NT6Jn3t9QmiWC86fRScJozlhMT6Pt-XULriMXeyRLaNHB-UeyMEQbY3fQ6R2SDquN0bd9mFmCO2h66IZVcKTWTUtKh_Cr35JOtxh0BEjbO22QKLcnGHyV9ggeSWsVNs-jJzU0Hl-M77Pox-eL78uvs8urL6vl4nJW8SLuZqLkNBe1gIIqlbFSUMpSrFnGY5HymqdCJWlWF4ylGdYKFU-gVlSkGHRlzZKz6PXBd9tYL8ewvIzDwwWjcR6I1YFQFm7k1ukQxF5a0PLvgnVrGZLRVYMyiwGEUkWdZ0laUlVCxnLMGVcFcMFF8Po07taXLaoKTeegmZhOvxi9kWu7kzyjgqc0GLwbDZwNUftOttpX2DRg0PbDuZNQTZpmw7nf_IM-fLuRWkO4gDa1DftWg6lcZCItYpHzIlDzB6gwFLa6Cn9WqDBOBe8ngsB0-LtbQ--9XF1_-3_26ueUfXvEbhCabuNt03faGj8F0wNYOeu9w_o-ZEbl0Bh3acihMeTYGEH26rhA96K7Tkj-AMGwBzc</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2222681027</pqid></control><display><type>article</type><title>"Smart Eye Camera": An innovative technique to evaluate tear film breakup time in a murine dry eye disease model</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Shimizu, Eisuke ; Ogawa, Yoko ; Yazu, Hiroyuki ; Aketa, Naohiko ; Yang, Fan ; Yamane, Mio ; Sato, Yasunori ; Kawakami, Yutaka ; Tsubota, Kazuo</creator><contributor>Lin, Meng C.</contributor><creatorcontrib>Shimizu, Eisuke ; Ogawa, Yoko ; Yazu, Hiroyuki ; Aketa, Naohiko ; Yang, Fan ; Yamane, Mio ; Sato, Yasunori ; Kawakami, Yutaka ; Tsubota, Kazuo ; Lin, Meng C.</creatorcontrib><description>Tear film breakup time (TFBUT) is an essential parameter used to diagnose dry eye disease (DED). However, a robust method for examining TFBUT in murine models has yet to be established. We invented an innovative device, namely, the "Smart Eye Camera", which addresses several problems associated with existing methods and is capable of evaluating TFBUT in a murine DED model. We compared images taken by existing devices and the Smart Eye Camera in a graft-versus-host disease-related DED murine model. We observed that the quality of the images obtained by the Smart Eye Camera were sufficient for practical use. Moreover, this new technique could be used to obtain measurements for several consecutive ocular phenotypes in a variety of environments. Here, we demonstrate the effectiveness of our new invention in the examination of ocular phenotypes, including TFBUT in a murine model. We highlight the potential for future translational studies adopting the Smart Eye Camera in clinical settings.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0215130</identifier><identifier>PMID: 31071120</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal models ; Animals ; Biology and Life Sciences ; Bone Marrow Transplantation - adverse effects ; Cameras ; Cameras (Photography) ; Cellular telephones ; Chronic fatigue syndrome ; Cornea - pathology ; Diagnosis ; Disease Models, Animal ; Dry eye syndromes ; Dry Eye Syndromes - diagnosis ; Dry Eye Syndromes - etiology ; Engineering and Technology ; Eye diseases ; Genotype & phenotype ; Graft vs Host Disease - complications ; Graft vs Host Disease - pathology ; Graft-versus-host reaction ; Health care ; Image quality ; Medical schools ; Medical technology ; Medicine ; Medicine and Health Sciences ; Mice ; Mice, Inbred BALB C ; Phenotype ; Phenotypes ; Photography - methods ; Printing, Three-Dimensional ; Research and Analysis Methods ; Scleroderma ; Serotonin ; Smartphones ; Tears - chemistry ; Tears - metabolism ; Transplants & implants</subject><ispartof>PloS one, 2019-05, Vol.14 (5), p.e0215130-e0215130</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Shimizu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Shimizu et al 2019 Shimizu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-8b6078f8a90dd51b80014ef1562846f648d345f91145efded63afd084e607bf13</citedby><cites>FETCH-LOGICAL-c692t-8b6078f8a90dd51b80014ef1562846f648d345f91145efded63afd084e607bf13</cites><orcidid>0000-0002-2906-9621 ; 0000-0002-3422-8578</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508640/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508640/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23853,27911,27912,53778,53780,79355,79356</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31071120$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lin, Meng C.</contributor><creatorcontrib>Shimizu, Eisuke</creatorcontrib><creatorcontrib>Ogawa, Yoko</creatorcontrib><creatorcontrib>Yazu, Hiroyuki</creatorcontrib><creatorcontrib>Aketa, Naohiko</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>Yamane, Mio</creatorcontrib><creatorcontrib>Sato, Yasunori</creatorcontrib><creatorcontrib>Kawakami, Yutaka</creatorcontrib><creatorcontrib>Tsubota, Kazuo</creatorcontrib><title>"Smart Eye Camera": An innovative technique to evaluate tear film breakup time in a murine dry eye disease model</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Tear film breakup time (TFBUT) is an essential parameter used to diagnose dry eye disease (DED). However, a robust method for examining TFBUT in murine models has yet to be established. We invented an innovative device, namely, the "Smart Eye Camera", which addresses several problems associated with existing methods and is capable of evaluating TFBUT in a murine DED model. We compared images taken by existing devices and the Smart Eye Camera in a graft-versus-host disease-related DED murine model. We observed that the quality of the images obtained by the Smart Eye Camera were sufficient for practical use. Moreover, this new technique could be used to obtain measurements for several consecutive ocular phenotypes in a variety of environments. Here, we demonstrate the effectiveness of our new invention in the examination of ocular phenotypes, including TFBUT in a murine model. We highlight the potential for future translational studies adopting the Smart Eye Camera in clinical settings.</description><subject>Animal models</subject><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Bone Marrow Transplantation - adverse effects</subject><subject>Cameras</subject><subject>Cameras (Photography)</subject><subject>Cellular telephones</subject><subject>Chronic fatigue syndrome</subject><subject>Cornea - pathology</subject><subject>Diagnosis</subject><subject>Disease Models, Animal</subject><subject>Dry eye syndromes</subject><subject>Dry Eye Syndromes - diagnosis</subject><subject>Dry Eye Syndromes - etiology</subject><subject>Engineering and Technology</subject><subject>Eye diseases</subject><subject>Genotype & phenotype</subject><subject>Graft vs Host Disease - complications</subject><subject>Graft vs Host Disease - pathology</subject><subject>Graft-versus-host reaction</subject><subject>Health care</subject><subject>Image quality</subject><subject>Medical schools</subject><subject>Medical technology</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Photography - methods</subject><subject>Printing, Three-Dimensional</subject><subject>Research and Analysis Methods</subject><subject>Scleroderma</subject><subject>Serotonin</subject><subject>Smartphones</subject><subject>Tears - chemistry</subject><subject>Tears - metabolism</subject><subject>Transplants & implants</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk19v0zAUxSMEYmPwDRBYQ0Lw0GLnj-PwgFRVAypNmsSAV-smvmk9Eruzk4p-exyaTQ3aA_FDLOd3ju1zc6PoJaNzluTsw43tnYFmvrUG5zRmGUvoo-iUFUk84zFNHh_NT6Jn3t9QmiWC86fRScJozlhMT6Pt-XULriMXeyRLaNHB-UeyMEQbY3fQ6R2SDquN0bd9mFmCO2h66IZVcKTWTUtKh_Cr35JOtxh0BEjbO22QKLcnGHyV9ggeSWsVNs-jJzU0Hl-M77Pox-eL78uvs8urL6vl4nJW8SLuZqLkNBe1gIIqlbFSUMpSrFnGY5HymqdCJWlWF4ylGdYKFU-gVlSkGHRlzZKz6PXBd9tYL8ewvIzDwwWjcR6I1YFQFm7k1ukQxF5a0PLvgnVrGZLRVYMyiwGEUkWdZ0laUlVCxnLMGVcFcMFF8Po07taXLaoKTeegmZhOvxi9kWu7kzyjgqc0GLwbDZwNUftOttpX2DRg0PbDuZNQTZpmw7nf_IM-fLuRWkO4gDa1DftWg6lcZCItYpHzIlDzB6gwFLa6Cn9WqDBOBe8ngsB0-LtbQ--9XF1_-3_26ueUfXvEbhCabuNt03faGj8F0wNYOeu9w_o-ZEbl0Bh3acihMeTYGEH26rhA96K7Tkj-AMGwBzc</recordid><startdate>20190509</startdate><enddate>20190509</enddate><creator>Shimizu, Eisuke</creator><creator>Ogawa, Yoko</creator><creator>Yazu, Hiroyuki</creator><creator>Aketa, Naohiko</creator><creator>Yang, Fan</creator><creator>Yamane, Mio</creator><creator>Sato, Yasunori</creator><creator>Kawakami, Yutaka</creator><creator>Tsubota, Kazuo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-2906-9621</orcidid><orcidid>https://orcid.org/0000-0002-3422-8578</orcidid></search><sort><creationdate>20190509</creationdate><title>"Smart Eye Camera": An innovative technique to evaluate tear film breakup time in a murine dry eye disease model</title><author>Shimizu, Eisuke ; Ogawa, Yoko ; Yazu, Hiroyuki ; Aketa, Naohiko ; Yang, Fan ; Yamane, Mio ; Sato, Yasunori ; Kawakami, Yutaka ; Tsubota, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-8b6078f8a90dd51b80014ef1562846f648d345f91145efded63afd084e607bf13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Biology and Life Sciences</topic><topic>Bone Marrow Transplantation - adverse effects</topic><topic>Cameras</topic><topic>Cameras (Photography)</topic><topic>Cellular telephones</topic><topic>Chronic fatigue syndrome</topic><topic>Cornea - pathology</topic><topic>Diagnosis</topic><topic>Disease Models, Animal</topic><topic>Dry eye syndromes</topic><topic>Dry Eye Syndromes - diagnosis</topic><topic>Dry Eye Syndromes - etiology</topic><topic>Engineering and Technology</topic><topic>Eye diseases</topic><topic>Genotype & phenotype</topic><topic>Graft vs Host Disease - complications</topic><topic>Graft vs Host Disease - pathology</topic><topic>Graft-versus-host reaction</topic><topic>Health care</topic><topic>Image quality</topic><topic>Medical schools</topic><topic>Medical technology</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Phenotype</topic><topic>Phenotypes</topic><topic>Photography - methods</topic><topic>Printing, Three-Dimensional</topic><topic>Research and Analysis Methods</topic><topic>Scleroderma</topic><topic>Serotonin</topic><topic>Smartphones</topic><topic>Tears - chemistry</topic><topic>Tears - metabolism</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimizu, Eisuke</creatorcontrib><creatorcontrib>Ogawa, Yoko</creatorcontrib><creatorcontrib>Yazu, Hiroyuki</creatorcontrib><creatorcontrib>Aketa, Naohiko</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>Yamane, Mio</creatorcontrib><creatorcontrib>Sato, Yasunori</creatorcontrib><creatorcontrib>Kawakami, Yutaka</creatorcontrib><creatorcontrib>Tsubota, Kazuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimizu, Eisuke</au><au>Ogawa, Yoko</au><au>Yazu, Hiroyuki</au><au>Aketa, Naohiko</au><au>Yang, Fan</au><au>Yamane, Mio</au><au>Sato, Yasunori</au><au>Kawakami, Yutaka</au><au>Tsubota, Kazuo</au><au>Lin, Meng C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>"Smart Eye Camera": An innovative technique to evaluate tear film breakup time in a murine dry eye disease model</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-05-09</date><risdate>2019</risdate><volume>14</volume><issue>5</issue><spage>e0215130</spage><epage>e0215130</epage><pages>e0215130-e0215130</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Tear film breakup time (TFBUT) is an essential parameter used to diagnose dry eye disease (DED). However, a robust method for examining TFBUT in murine models has yet to be established. We invented an innovative device, namely, the "Smart Eye Camera", which addresses several problems associated with existing methods and is capable of evaluating TFBUT in a murine DED model. We compared images taken by existing devices and the Smart Eye Camera in a graft-versus-host disease-related DED murine model. We observed that the quality of the images obtained by the Smart Eye Camera were sufficient for practical use. Moreover, this new technique could be used to obtain measurements for several consecutive ocular phenotypes in a variety of environments. Here, we demonstrate the effectiveness of our new invention in the examination of ocular phenotypes, including TFBUT in a murine model. We highlight the potential for future translational studies adopting the Smart Eye Camera in clinical settings.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31071120</pmid><doi>10.1371/journal.pone.0215130</doi><tpages>e0215130</tpages><orcidid>https://orcid.org/0000-0002-2906-9621</orcidid><orcidid>https://orcid.org/0000-0002-3422-8578</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-05, Vol.14 (5), p.e0215130-e0215130 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2222681027 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animal models Animals Biology and Life Sciences Bone Marrow Transplantation - adverse effects Cameras Cameras (Photography) Cellular telephones Chronic fatigue syndrome Cornea - pathology Diagnosis Disease Models, Animal Dry eye syndromes Dry Eye Syndromes - diagnosis Dry Eye Syndromes - etiology Engineering and Technology Eye diseases Genotype & phenotype Graft vs Host Disease - complications Graft vs Host Disease - pathology Graft-versus-host reaction Health care Image quality Medical schools Medical technology Medicine Medicine and Health Sciences Mice Mice, Inbred BALB C Phenotype Phenotypes Photography - methods Printing, Three-Dimensional Research and Analysis Methods Scleroderma Serotonin Smartphones Tears - chemistry Tears - metabolism Transplants & implants |
| title | "Smart Eye Camera": An innovative technique to evaluate tear film breakup time in a murine dry eye disease model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T04%3A09%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%22Smart%20Eye%20Camera%22:%20An%20innovative%20technique%20to%20evaluate%20tear%20film%20breakup%20time%20in%20a%20murine%20dry%20eye%20disease%20model&rft.jtitle=PloS%20one&rft.au=Shimizu,%20Eisuke&rft.date=2019-05-09&rft.volume=14&rft.issue=5&rft.spage=e0215130&rft.epage=e0215130&rft.pages=e0215130-e0215130&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0215130&rft_dat=%3Cgale_plos_%3EA584928769%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2222681027&rft_id=info:pmid/31071120&rft_galeid=A584928769&rft_doaj_id=oai_doaj_org_article_52aa8dd9f7534b0dba517e716d9a6868&rfr_iscdi=true |