Assessment on reticuloendotheliosis virus infection in specific-pathogen-free chickens based on detection of yolk antibody
Reticuloendotheliosis virus (REV) is the most frequent exogenous virus that contaminates attenuated vaccines. Therefore, it is extremely important to select REV-free specific-pathogen-free (SPF) chicken embryos. Generally, REV infection is assessed by detecting REV antibodies in SPF chickens. This p...
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| creator | Li, Yang Wang, Tuanjie Wang, Lin Sun, Mingjun Cui, Zhizhong Chang, Shuang Wu, Yongping Zhang, Xiaodong Yu, Xiaohui Sun, Tao Zhao, Peng |
| description | Reticuloendotheliosis virus (REV) is the most frequent exogenous virus that contaminates attenuated vaccines. Therefore, it is extremely important to select REV-free specific-pathogen-free (SPF) chicken embryos. Generally, REV infection is assessed by detecting REV antibodies in SPF chickens. This present study seeks to evaluate REV infection by replacing serum antibody detection with yolk antibody detection. A cohort of 40 nineteen-week-old SPF chickens were artificially inoculated with REV, with 32 SPF chickens raised in another isolation environment served as a blank control. Eggs and serum from 23-week-old chickens were sampled, and yolks were diluted separately to ratios of 1:150, 1:200, 1:300 and 1:400, which were detected together with serum. We found that the yolk antibody detection findings at a dilution of 1:300 had the highest coincidence rate compared with that based on serum antibody measurements. At a dilution ratio of 1:300 for yolk antibody, 72 chickens were continuously observed for 10 weeks from 25- to 34-weeks-old. Our findings were based on serum antibody or yolk antibody detection, and the evaluation results were completely consistent. Therefore, all serum antibody-positive chickens were yolk antibody-positive, and vice versa. Accordingly, vaccine producers can estimate REV cleanliness in a poultry farm by sampling yolk antibody titers. |
| doi_str_mv | 10.1371/journal.pone.0213978 |
| format | Article |
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Therefore, it is extremely important to select REV-free specific-pathogen-free (SPF) chicken embryos. Generally, REV infection is assessed by detecting REV antibodies in SPF chickens. This present study seeks to evaluate REV infection by replacing serum antibody detection with yolk antibody detection. A cohort of 40 nineteen-week-old SPF chickens were artificially inoculated with REV, with 32 SPF chickens raised in another isolation environment served as a blank control. Eggs and serum from 23-week-old chickens were sampled, and yolks were diluted separately to ratios of 1:150, 1:200, 1:300 and 1:400, which were detected together with serum. We found that the yolk antibody detection findings at a dilution of 1:300 had the highest coincidence rate compared with that based on serum antibody measurements. At a dilution ratio of 1:300 for yolk antibody, 72 chickens were continuously observed for 10 weeks from 25- to 34-weeks-old. Our findings were based on serum antibody or yolk antibody detection, and the evaluation results were completely consistent. Therefore, all serum antibody-positive chickens were yolk antibody-positive, and vice versa. Accordingly, vaccine producers can estimate REV cleanliness in a poultry farm by sampling yolk antibody titers.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0213978</identifier><identifier>PMID: 31009463</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antibodies ; Antibodies, Viral - isolation & purification ; Chick Embryo ; Chickens ; Chickens - virology ; Dilution ; Eggs ; Embryos ; Epidemiology ; Infection ; Infections ; Li, Yang ; Livestock farms ; Medical research ; Pathogens ; Patient outcomes ; Poultry ; Poultry Diseases - diagnosis ; Poultry Diseases - virology ; Poultry farming ; Reticuloendotheliosis ; Reticuloendotheliosis virus - immunology ; Reticuloendotheliosis virus - isolation & purification ; Specific Pathogen-Free Organisms ; Vaccines ; Vaccines, Attenuated ; Veterinary colleges ; Virus Cultivation - methods ; Virus Cultivation - veterinary ; Virus diseases ; Viruses ; Yolk ; Yolk Sac - virology</subject><ispartof>PloS one, 2019-04, Vol.14 (4), p.e0213978-e0213978</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Li et al 2019 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c641t-8252b59941ce54062ca83ff61fa082bcc27ad2d0037c149be4df0fcaab2ce7b03</cites><orcidid>0000-0003-0555-8727</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476468/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476468/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31009463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lau, Eric HY</contributor><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Wang, Tuanjie</creatorcontrib><creatorcontrib>Wang, Lin</creatorcontrib><creatorcontrib>Sun, Mingjun</creatorcontrib><creatorcontrib>Cui, Zhizhong</creatorcontrib><creatorcontrib>Chang, Shuang</creatorcontrib><creatorcontrib>Wu, Yongping</creatorcontrib><creatorcontrib>Zhang, Xiaodong</creatorcontrib><creatorcontrib>Yu, Xiaohui</creatorcontrib><creatorcontrib>Sun, Tao</creatorcontrib><creatorcontrib>Zhao, Peng</creatorcontrib><title>Assessment on reticuloendotheliosis virus infection in specific-pathogen-free chickens based on detection of yolk antibody</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Reticuloendotheliosis virus (REV) is the most frequent exogenous virus that contaminates attenuated vaccines. Therefore, it is extremely important to select REV-free specific-pathogen-free (SPF) chicken embryos. Generally, REV infection is assessed by detecting REV antibodies in SPF chickens. This present study seeks to evaluate REV infection by replacing serum antibody detection with yolk antibody detection. A cohort of 40 nineteen-week-old SPF chickens were artificially inoculated with REV, with 32 SPF chickens raised in another isolation environment served as a blank control. Eggs and serum from 23-week-old chickens were sampled, and yolks were diluted separately to ratios of 1:150, 1:200, 1:300 and 1:400, which were detected together with serum. We found that the yolk antibody detection findings at a dilution of 1:300 had the highest coincidence rate compared with that based on serum antibody measurements. At a dilution ratio of 1:300 for yolk antibody, 72 chickens were continuously observed for 10 weeks from 25- to 34-weeks-old. Our findings were based on serum antibody or yolk antibody detection, and the evaluation results were completely consistent. Therefore, all serum antibody-positive chickens were yolk antibody-positive, and vice versa. Accordingly, vaccine producers can estimate REV cleanliness in a poultry farm by sampling yolk antibody titers.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Viral - isolation & purification</subject><subject>Chick Embryo</subject><subject>Chickens</subject><subject>Chickens - virology</subject><subject>Dilution</subject><subject>Eggs</subject><subject>Embryos</subject><subject>Epidemiology</subject><subject>Infection</subject><subject>Infections</subject><subject>Li, Yang</subject><subject>Livestock farms</subject><subject>Medical research</subject><subject>Pathogens</subject><subject>Patient outcomes</subject><subject>Poultry</subject><subject>Poultry Diseases - diagnosis</subject><subject>Poultry Diseases - virology</subject><subject>Poultry farming</subject><subject>Reticuloendotheliosis</subject><subject>Reticuloendotheliosis virus - immunology</subject><subject>Reticuloendotheliosis virus - isolation & purification</subject><subject>Specific Pathogen-Free Organisms</subject><subject>Vaccines</subject><subject>Vaccines, Attenuated</subject><subject>Veterinary colleges</subject><subject>Virus Cultivation - methods</subject><subject>Virus Cultivation - veterinary</subject><subject>Virus diseases</subject><subject>Viruses</subject><subject>Yolk</subject><subject>Yolk Sac - virology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLguhFxyTtpO2NMCx-DCws-HUb0uRkmt1MMibp4vjrTZ3uMpW9kF60pM_7npw3OVn2HKMFLmv87soN3nKz2DkLC0Rw2dbNg-wUtyUpKEHlw6Pvk-xJCFcILcuG0sfZSYkRaitanma_VyFACFuwMXc29xC1GIwDK13swWgXdMhvtB9Crq0CEXWitM3DDoRWWhQ7Hnu3AVsoD5CLXotrsCHveAA5OkqIk8qpfO_Mdc5t1J2T-6fZI8VNgGfT-yz7_vHDt_PPxcXlp_X56qIQtMKxaMiSdMu2rbCAZYUoEbwplaJYcdSQTghSc0kkQmUtcNV2UEmFlOC8IwLqDpVn2cuD7864wKbYAiMpMkqrtmkTsT4Q0vErtvN6y_2eOa7Z3wXnN4z7FIwBJgiRSnW0oalQ18qmrpUAKoHLrm3w6PV-qjZ0W5AiBeu5mZnO_1jds427YbSqaUWbZPBmMvDu5wAhsq0OAozhFtxw2DeuaozHzl79g97f3URteGognaJLdcVoylbLpiQYt3WdqMU9VHokbLVId0zptD4TvJ0JEhPhV9zwIQS2_vrl_9nLH3P29RHbAzexD84M4x0Kc7A6gMK7EDyou5AxYuOI3KbBxhFh04gk2YvjA7oT3c5E-Qf4SA-g</recordid><startdate>20190422</startdate><enddate>20190422</enddate><creator>Li, Yang</creator><creator>Wang, Tuanjie</creator><creator>Wang, Lin</creator><creator>Sun, Mingjun</creator><creator>Cui, Zhizhong</creator><creator>Chang, Shuang</creator><creator>Wu, Yongping</creator><creator>Zhang, Xiaodong</creator><creator>Yu, Xiaohui</creator><creator>Sun, Tao</creator><creator>Zhao, Peng</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-0555-8727</orcidid></search><sort><creationdate>20190422</creationdate><title>Assessment on reticuloendotheliosis virus infection in specific-pathogen-free chickens based on detection of yolk antibody</title><author>Li, Yang ; Wang, Tuanjie ; Wang, Lin ; Sun, Mingjun ; Cui, Zhizhong ; Chang, Shuang ; Wu, Yongping ; Zhang, Xiaodong ; Yu, Xiaohui ; Sun, Tao ; Zhao, Peng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c641t-8252b59941ce54062ca83ff61fa082bcc27ad2d0037c149be4df0fcaab2ce7b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Viral - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yang</au><au>Wang, Tuanjie</au><au>Wang, Lin</au><au>Sun, Mingjun</au><au>Cui, Zhizhong</au><au>Chang, Shuang</au><au>Wu, Yongping</au><au>Zhang, Xiaodong</au><au>Yu, Xiaohui</au><au>Sun, Tao</au><au>Zhao, Peng</au><au>Lau, Eric HY</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment on reticuloendotheliosis virus infection in specific-pathogen-free chickens based on detection of yolk antibody</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-04-22</date><risdate>2019</risdate><volume>14</volume><issue>4</issue><spage>e0213978</spage><epage>e0213978</epage><pages>e0213978-e0213978</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Reticuloendotheliosis virus (REV) is the most frequent exogenous virus that contaminates attenuated vaccines. Therefore, it is extremely important to select REV-free specific-pathogen-free (SPF) chicken embryos. Generally, REV infection is assessed by detecting REV antibodies in SPF chickens. This present study seeks to evaluate REV infection by replacing serum antibody detection with yolk antibody detection. A cohort of 40 nineteen-week-old SPF chickens were artificially inoculated with REV, with 32 SPF chickens raised in another isolation environment served as a blank control. Eggs and serum from 23-week-old chickens were sampled, and yolks were diluted separately to ratios of 1:150, 1:200, 1:300 and 1:400, which were detected together with serum. We found that the yolk antibody detection findings at a dilution of 1:300 had the highest coincidence rate compared with that based on serum antibody measurements. At a dilution ratio of 1:300 for yolk antibody, 72 chickens were continuously observed for 10 weeks from 25- to 34-weeks-old. Our findings were based on serum antibody or yolk antibody detection, and the evaluation results were completely consistent. Therefore, all serum antibody-positive chickens were yolk antibody-positive, and vice versa. Accordingly, vaccine producers can estimate REV cleanliness in a poultry farm by sampling yolk antibody titers.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31009463</pmid><doi>10.1371/journal.pone.0213978</doi><tpages>e0213978</tpages><orcidid>https://orcid.org/0000-0003-0555-8727</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2019-04, Vol.14 (4), p.e0213978-e0213978 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2213664989 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies Antibodies, Viral - isolation & purification Chick Embryo Chickens Chickens - virology Dilution Eggs Embryos Epidemiology Infection Infections Li, Yang Livestock farms Medical research Pathogens Patient outcomes Poultry Poultry Diseases - diagnosis Poultry Diseases - virology Poultry farming Reticuloendotheliosis Reticuloendotheliosis virus - immunology Reticuloendotheliosis virus - isolation & purification Specific Pathogen-Free Organisms Vaccines Vaccines, Attenuated Veterinary colleges Virus Cultivation - methods Virus Cultivation - veterinary Virus diseases Viruses Yolk Yolk Sac - virology |
| title | Assessment on reticuloendotheliosis virus infection in specific-pathogen-free chickens based on detection of yolk antibody |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T11%3A34%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Assessment%20on%20reticuloendotheliosis%20virus%20infection%20in%20specific-pathogen-free%20chickens%20based%20on%20detection%20of%20yolk%20antibody&rft.jtitle=PloS%20one&rft.au=Li,%20Yang&rft.date=2019-04-22&rft.volume=14&rft.issue=4&rft.spage=e0213978&rft.epage=e0213978&rft.pages=e0213978-e0213978&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0213978&rft_dat=%3Cgale_plos_%3EA583211977%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2213664989&rft_id=info:pmid/31009463&rft_galeid=A583211977&rft_doaj_id=oai_doaj_org_article_c22dffb686be4b9d877fce6deadb9819&rfr_iscdi=true |