A novel electron emission-based cell culture device promotes cell proliferation and differentiation of pre-osteoblastic MC3T3-E1 cells
In this report we demonstrate the effect of a novel electron emission-based cell culture device on the proliferation and differentiation of pre-osteoblastic MC3T3-E1 cells. Our device has an electron emission element that allows, for the first time, stable emission of electrons into an atmosphere. A...
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| Veröffentlicht in: | PloS one 2019-03, Vol.14 (3), p.e0213579-e0213579 |
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| creator | Sugimori, Fumiaki Hirakawa, Hiroyuki Tsutsui, Ai Yamaji, Hiroyuki Komaru, Shohei Takasaki, Mai Iwamatsu, Tadashi Uemura, Toshimasa Uemura, Yo Morita, Kenichi Tsumura, Takashi |
| description | In this report we demonstrate the effect of a novel electron emission-based cell culture device on the proliferation and differentiation of pre-osteoblastic MC3T3-E1 cells. Our device has an electron emission element that allows, for the first time, stable emission of electrons into an atmosphere. Atmospheric electrons react with gas molecules to generate radicals and negative ions, which induce a variety of biochemical reactions in the attached cell culture system. In this study, we demonstrated the effect of this new electron emission-based cell culture device on cell proliferation and differentiation using pre-osteoblastic MC3T3-E1 cells. Electron emission stimulation (EES) was applied directly to culture medium containing plated cells, after which the number of living cells, the mRNA levels of osteogenesis-related genes, and the alkaline phosphatase (ALP) activity were evaluated. The growth rate of EES-exposed cells increased by approximately 20% in comparison with unexposed control cells. We also found the mRNA levels of osteogenic specific genes such as collagen type I α-1, core-binding factor α-1, and osteocalcin to be up-regulated following EES. ALP activity, a marker for osteogenic activity, was significantly enhanced in EES-treated cells. Furthermore, reactive oxygen species generated by EES were measured to determine their effect on MC3T3-E1 cells. These results suggest that our new electron emission-based cell culture device, while providing a relatively weak stimulus in comparison with atmospheric plasma systems, promotes cell proliferation and differentiation. This system is expected to find application in regenerative medicine, specifically in relation to bone regeneration. |
| doi_str_mv | 10.1371/journal.pone.0213579 |
| format | Article |
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Our device has an electron emission element that allows, for the first time, stable emission of electrons into an atmosphere. Atmospheric electrons react with gas molecules to generate radicals and negative ions, which induce a variety of biochemical reactions in the attached cell culture system. In this study, we demonstrated the effect of this new electron emission-based cell culture device on cell proliferation and differentiation using pre-osteoblastic MC3T3-E1 cells. Electron emission stimulation (EES) was applied directly to culture medium containing plated cells, after which the number of living cells, the mRNA levels of osteogenesis-related genes, and the alkaline phosphatase (ALP) activity were evaluated. The growth rate of EES-exposed cells increased by approximately 20% in comparison with unexposed control cells. We also found the mRNA levels of osteogenic specific genes such as collagen type I α-1, core-binding factor α-1, and osteocalcin to be up-regulated following EES. ALP activity, a marker for osteogenic activity, was significantly enhanced in EES-treated cells. Furthermore, reactive oxygen species generated by EES were measured to determine their effect on MC3T3-E1 cells. These results suggest that our new electron emission-based cell culture device, while providing a relatively weak stimulus in comparison with atmospheric plasma systems, promotes cell proliferation and differentiation. This system is expected to find application in regenerative medicine, specifically in relation to bone regeneration.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0213579</identifier><identifier>PMID: 30921357</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Alkaline phosphatase ; Aluminum ; Analysis ; Animals ; Antigens, Differentiation - biosynthesis ; Atmosphere ; Atmospheric pressure ; Biochemistry ; Biocompatibility ; Biology and Life Sciences ; Biotechnology ; Bone growth ; Cell culture ; Cell Culture Techniques - instrumentation ; Cell Culture Techniques - methods ; Cell differentiation ; Cell Line ; Cell Proliferation ; Collagen ; Collagen (type I) ; Cooperation ; Differentiation ; EDTA ; Electric fields ; Electrodes ; Electromagnetic radiation ; Electron emission ; Electrons ; Emission analysis ; Emissions ; Gene expression ; Genes ; Growth rate ; Medicine and Health Sciences ; Messenger RNA ; Mice ; mRNA ; Negative ions ; Novels ; Osteoblastogenesis ; Osteoblasts ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Osteocalcin ; Osteogenesis ; Oxygen ; Phosphatases ; Plasma Gases - chemistry ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Regeneration (physiology) ; Regenerative medicine ; Research and Analysis Methods ; RNA</subject><ispartof>PloS one, 2019-03, Vol.14 (3), p.e0213579-e0213579</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Sugimori et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Our device has an electron emission element that allows, for the first time, stable emission of electrons into an atmosphere. Atmospheric electrons react with gas molecules to generate radicals and negative ions, which induce a variety of biochemical reactions in the attached cell culture system. In this study, we demonstrated the effect of this new electron emission-based cell culture device on cell proliferation and differentiation using pre-osteoblastic MC3T3-E1 cells. Electron emission stimulation (EES) was applied directly to culture medium containing plated cells, after which the number of living cells, the mRNA levels of osteogenesis-related genes, and the alkaline phosphatase (ALP) activity were evaluated. The growth rate of EES-exposed cells increased by approximately 20% in comparison with unexposed control cells. We also found the mRNA levels of osteogenic specific genes such as collagen type I α-1, core-binding factor α-1, and osteocalcin to be up-regulated following EES. 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This system is expected to find application in regenerative medicine, specifically in relation to bone regeneration.</description><subject>Alkaline phosphatase</subject><subject>Aluminum</subject><subject>Analysis</subject><subject>Animals</subject><subject>Antigens, Differentiation - biosynthesis</subject><subject>Atmosphere</subject><subject>Atmospheric pressure</subject><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Bone growth</subject><subject>Cell culture</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell differentiation</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>Collagen</subject><subject>Collagen (type I)</subject><subject>Cooperation</subject><subject>Differentiation</subject><subject>EDTA</subject><subject>Electric fields</subject><subject>Electrodes</subject><subject>Electromagnetic radiation</subject><subject>Electron emission</subject><subject>Electrons</subject><subject>Emission analysis</subject><subject>Emissions</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Growth rate</subject><subject>Medicine and Health Sciences</subject><subject>Messenger RNA</subject><subject>Mice</subject><subject>mRNA</subject><subject>Negative ions</subject><subject>Novels</subject><subject>Osteoblastogenesis</subject><subject>Osteoblasts</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Osteocalcin</subject><subject>Osteogenesis</subject><subject>Oxygen</subject><subject>Phosphatases</subject><subject>Plasma Gases - chemistry</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Regeneration (physiology)</subject><subject>Regenerative medicine</subject><subject>Research and Analysis Methods</subject><subject>RNA</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99u0zAUxiMEYmPwBggiISG4SPGfxE5ukKpqQKWhSTC4tRznuE3lxp3tVPACPDdOk00t2gXKhePj3_fZ59gnSV5iNMOU4w8b27tOmtnOdjBDBNOCV4-Sc1xRkjGC6OOj_7PkmfcbhApaMvY0OaOoOgjOkz_ztLN7MCkYUMHZLoVt631ru6yWHppUgTGp6k3oHaQN7FsF6c7ZrQ3gx8U4M60GJ0NUpbJr0qbVcQ5daMeY1RGCzPoAtjbSh1alXxf0hmaX-ODhnydPtDQeXkzjRfLj0-XN4kt2df15uZhfZYpVJGS0zlXJtCyx5jTXFSp5URJoKoaKHFcFQ4SVhdYKEUWLumBUYSYBK86kblBJL5LXo-_OWC-mEnpBCEKEMMyrSCxHorFyI3au3Ur3W1jZikPAupWQLiZgQHBOEC8VVKhGeYlqSRRoLmXV1JBrpKPXx2m3vt5Co2JFnDQnpqcrXbsWK7sXLKdlTCwavJsMnL3twQcRL2comOzA9uO5OeeYDOd-8w_6cHYTtZIxgbbTNu6rBlMxL0qUU4RzGqnZA1T8mvg4VHxvuo3xE8H7E0FkAvwKK9l7L5bfv_0_e_3zlH17xK5BmrD21vTDq_KnYD6CylnvHej7ImMkhna5q4YY2kVM7RJlr44v6F501x_0LxaUEGQ</recordid><startdate>20190328</startdate><enddate>20190328</enddate><creator>Sugimori, Fumiaki</creator><creator>Hirakawa, Hiroyuki</creator><creator>Tsutsui, Ai</creator><creator>Yamaji, Hiroyuki</creator><creator>Komaru, Shohei</creator><creator>Takasaki, Mai</creator><creator>Iwamatsu, Tadashi</creator><creator>Uemura, Toshimasa</creator><creator>Uemura, Yo</creator><creator>Morita, Kenichi</creator><creator>Tsumura, Takashi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-7670-1426</orcidid><orcidid>https://orcid.org/0000-0002-0588-6117</orcidid></search><sort><creationdate>20190328</creationdate><title>A novel electron emission-based cell culture device promotes cell proliferation and differentiation of pre-osteoblastic MC3T3-E1 cells</title><author>Sugimori, Fumiaki ; Hirakawa, Hiroyuki ; Tsutsui, Ai ; Yamaji, Hiroyuki ; Komaru, Shohei ; Takasaki, Mai ; Iwamatsu, Tadashi ; Uemura, Toshimasa ; Uemura, Yo ; Morita, Kenichi ; Tsumura, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-3b4c86fa81f734f9087582ed96054195602685ffc02c35b563c16ae1c76afd083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Alkaline phosphatase</topic><topic>Aluminum</topic><topic>Analysis</topic><topic>Animals</topic><topic>Antigens, Differentiation - 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Our device has an electron emission element that allows, for the first time, stable emission of electrons into an atmosphere. Atmospheric electrons react with gas molecules to generate radicals and negative ions, which induce a variety of biochemical reactions in the attached cell culture system. In this study, we demonstrated the effect of this new electron emission-based cell culture device on cell proliferation and differentiation using pre-osteoblastic MC3T3-E1 cells. Electron emission stimulation (EES) was applied directly to culture medium containing plated cells, after which the number of living cells, the mRNA levels of osteogenesis-related genes, and the alkaline phosphatase (ALP) activity were evaluated. The growth rate of EES-exposed cells increased by approximately 20% in comparison with unexposed control cells. We also found the mRNA levels of osteogenic specific genes such as collagen type I α-1, core-binding factor α-1, and osteocalcin to be up-regulated following EES. ALP activity, a marker for osteogenic activity, was significantly enhanced in EES-treated cells. Furthermore, reactive oxygen species generated by EES were measured to determine their effect on MC3T3-E1 cells. These results suggest that our new electron emission-based cell culture device, while providing a relatively weak stimulus in comparison with atmospheric plasma systems, promotes cell proliferation and differentiation. This system is expected to find application in regenerative medicine, specifically in relation to bone regeneration.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30921357</pmid><doi>10.1371/journal.pone.0213579</doi><tpages>e0213579</tpages><orcidid>https://orcid.org/0000-0001-7670-1426</orcidid><orcidid>https://orcid.org/0000-0002-0588-6117</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_2200226179 |
| source | Open Access: PubMed Central; MEDLINE; Public Library of Science; Directory of Open Access Journals; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Alkaline phosphatase Aluminum Analysis Animals Antigens, Differentiation - biosynthesis Atmosphere Atmospheric pressure Biochemistry Biocompatibility Biology and Life Sciences Biotechnology Bone growth Cell culture Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cell differentiation Cell Line Cell Proliferation Collagen Collagen (type I) Cooperation Differentiation EDTA Electric fields Electrodes Electromagnetic radiation Electron emission Electrons Emission analysis Emissions Gene expression Genes Growth rate Medicine and Health Sciences Messenger RNA Mice mRNA Negative ions Novels Osteoblastogenesis Osteoblasts Osteoblasts - cytology Osteoblasts - metabolism Osteocalcin Osteogenesis Oxygen Phosphatases Plasma Gases - chemistry Reactive oxygen species Reactive Oxygen Species - metabolism Regeneration (physiology) Regenerative medicine Research and Analysis Methods RNA |
| title | A novel electron emission-based cell culture device promotes cell proliferation and differentiation of pre-osteoblastic MC3T3-E1 cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T09%3A49%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20novel%20electron%20emission-based%20cell%20culture%20device%20promotes%20cell%20proliferation%20and%20differentiation%20of%20pre-osteoblastic%20MC3T3-E1%20cells&rft.jtitle=PloS%20one&rft.au=Sugimori,%20Fumiaki&rft.date=2019-03-28&rft.volume=14&rft.issue=3&rft.spage=e0213579&rft.epage=e0213579&rft.pages=e0213579-e0213579&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0213579&rft_dat=%3Cgale_plos_%3EA580430143%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2200226179&rft_id=info:pmid/30921357&rft_galeid=A580430143&rft_doaj_id=oai_doaj_org_article_772078ce90b0480ba2cef7aa9dbe4f0f&rfr_iscdi=true |