Analysis of the dysregulation between regulatory B and T cells (Breg and Treg) in human immunodeficiency virus (HIV)-infected patients
This study examines the relationship between regulatory B (Breg) and T (Treg) compartments, which play crucial roles in the maintenance of immune homeostasis in the context of HIV. Using flow cytometry, the phenotypes of different Breg and Treg subsets from HIV-infected and healthy individuals were...
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| Veröffentlicht in: | PloS one 2019-03, Vol.14 (3), p.e0213744-e0213744 |
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| creator | Gutiérrez, Carolina Lopez-Abente, Jacobo Pérez-Fernández, Verónica Prieto-Sánchez, Adrián Correa-Rocha, Rafael Moreno-Guillen, Santiago Muñoz-Fernández, María-Ángeles Pion, Marjorie |
| description | This study examines the relationship between regulatory B (Breg) and T (Treg) compartments, which play crucial roles in the maintenance of immune homeostasis in the context of HIV. Using flow cytometry, the phenotypes of different Breg and Treg subsets from HIV-infected and healthy individuals were analyzed, along with the suppressive capacity of Breg. Peripheral blood samples of thirteen HIV+ treatment-naïve individuals, fourteen treated-HIV+ individuals with undetectable viral load and twelve healthy individuals were analyzed. The absolute counts of Breg and Treg subsets were decreased in HIV+ treatment-naïve individuals in comparison to treated-HIV+ and healthy individuals. Interestingly, correlations between Breg subsets (CD24hiCD27+ and PD-L1+ B cells) and IL-10-producing Breg observed in healthy individuals were lost in HIV+ treatment-naïve individuals. However, a correlation between frequencies of CD24hiCD38hi or TIM-1+-Breg subsets and Treg was observed in HIV+ treatment-naïve individuals and not in healthy individuals. Therefore, we hypothesized that various Breg subsets might have different functions during B and T-cell homeostasis during HIV-1 infection. In parallel, stimulated Breg from HIV-infected treatment-naïve individuals presented a decreased ability to suppress CD4+ T-cell proliferation in comparison to the stimulated Breg from treated-HIV+ or healthy individuals. We demonstrate a dysregulation between Breg and Treg subsets in HIV-infected individuals, which might participate in the hyper-activation and exhaustion of the immune system that occurs in such patients. |
| doi_str_mv | 10.1371/journal.pone.0213744 |
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Using flow cytometry, the phenotypes of different Breg and Treg subsets from HIV-infected and healthy individuals were analyzed, along with the suppressive capacity of Breg. Peripheral blood samples of thirteen HIV+ treatment-naïve individuals, fourteen treated-HIV+ individuals with undetectable viral load and twelve healthy individuals were analyzed. The absolute counts of Breg and Treg subsets were decreased in HIV+ treatment-naïve individuals in comparison to treated-HIV+ and healthy individuals. Interestingly, correlations between Breg subsets (CD24hiCD27+ and PD-L1+ B cells) and IL-10-producing Breg observed in healthy individuals were lost in HIV+ treatment-naïve individuals. However, a correlation between frequencies of CD24hiCD38hi or TIM-1+-Breg subsets and Treg was observed in HIV+ treatment-naïve individuals and not in healthy individuals. Therefore, we hypothesized that various Breg subsets might have different functions during B and T-cell homeostasis during HIV-1 infection. In parallel, stimulated Breg from HIV-infected treatment-naïve individuals presented a decreased ability to suppress CD4+ T-cell proliferation in comparison to the stimulated Breg from treated-HIV+ or healthy individuals. We demonstrate a dysregulation between Breg and Treg subsets in HIV-infected individuals, which might participate in the hyper-activation and exhaustion of the immune system that occurs in such patients.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0213744</identifier><identifier>PMID: 30917149</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Arthritis ; B-Lymphocyte Subsets - cytology ; B-Lymphocyte Subsets - immunology ; B-Lymphocyte Subsets - metabolism ; B-Lymphocytes, Regulatory - cytology ; B-Lymphocytes, Regulatory - immunology ; B-Lymphocytes, Regulatory - metabolism ; Biology and Life Sciences ; Breast cancer ; Cardiovascular disease ; Case-Control Studies ; CD4 antigen ; Cell Proliferation ; Correlation ; Dendritic cells ; Exhaustion ; Female ; Flow cytometry ; HIV ; HIV - isolation & purification ; HIV - physiology ; HIV Infections - immunology ; HIV Infections - pathology ; Homeostasis ; Hospitals ; Human immunodeficiency virus ; Humans ; Immune system ; Infections ; Infectious diseases ; Inflammation ; Interleukin 10 ; Interleukin-10 - metabolism ; Laboratories ; Leukocytes, Mononuclear - cytology ; Leukocytes, Mononuclear - metabolism ; Ligands ; Lymphocytes ; Lymphocytes B ; Lymphocytes T ; Male ; Medical research ; Medicine ; Medicine and Health Sciences ; Middle Aged ; Patients ; PD-L1 protein ; Peripheral blood ; Phenotype ; Phenotypes ; Research and Analysis Methods ; Surgery ; T-Lymphocytes, Regulatory - cytology ; T-Lymphocytes, Regulatory - immunology ; T-Lymphocytes, Regulatory - metabolism ; Viral Load ; Viruses</subject><ispartof>PloS one, 2019-03, Vol.14 (3), p.e0213744-e0213744</ispartof><rights>2019 Gutiérrez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Gutiérrez et al 2019 Gutiérrez et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-f417a656a224bef85b8a3372694fd981d0dcc0f498eb9b63c4ebbd2f865bcbfa3</citedby><cites>FETCH-LOGICAL-c526t-f417a656a224bef85b8a3372694fd981d0dcc0f498eb9b63c4ebbd2f865bcbfa3</cites><orcidid>0000-0002-6406-747X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436717/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436717/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30917149$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kim, Chang H.</contributor><creatorcontrib>Gutiérrez, Carolina</creatorcontrib><creatorcontrib>Lopez-Abente, Jacobo</creatorcontrib><creatorcontrib>Pérez-Fernández, Verónica</creatorcontrib><creatorcontrib>Prieto-Sánchez, Adrián</creatorcontrib><creatorcontrib>Correa-Rocha, Rafael</creatorcontrib><creatorcontrib>Moreno-Guillen, Santiago</creatorcontrib><creatorcontrib>Muñoz-Fernández, María-Ángeles</creatorcontrib><creatorcontrib>Pion, Marjorie</creatorcontrib><title>Analysis of the dysregulation between regulatory B and T cells (Breg and Treg) in human immunodeficiency virus (HIV)-infected patients</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>This study examines the relationship between regulatory B (Breg) and T (Treg) compartments, which play crucial roles in the maintenance of immune homeostasis in the context of HIV. Using flow cytometry, the phenotypes of different Breg and Treg subsets from HIV-infected and healthy individuals were analyzed, along with the suppressive capacity of Breg. Peripheral blood samples of thirteen HIV+ treatment-naïve individuals, fourteen treated-HIV+ individuals with undetectable viral load and twelve healthy individuals were analyzed. The absolute counts of Breg and Treg subsets were decreased in HIV+ treatment-naïve individuals in comparison to treated-HIV+ and healthy individuals. Interestingly, correlations between Breg subsets (CD24hiCD27+ and PD-L1+ B cells) and IL-10-producing Breg observed in healthy individuals were lost in HIV+ treatment-naïve individuals. However, a correlation between frequencies of CD24hiCD38hi or TIM-1+-Breg subsets and Treg was observed in HIV+ treatment-naïve individuals and not in healthy individuals. Therefore, we hypothesized that various Breg subsets might have different functions during B and T-cell homeostasis during HIV-1 infection. In parallel, stimulated Breg from HIV-infected treatment-naïve individuals presented a decreased ability to suppress CD4+ T-cell proliferation in comparison to the stimulated Breg from treated-HIV+ or healthy individuals. We demonstrate a dysregulation between Breg and Treg subsets in HIV-infected individuals, which might participate in the hyper-activation and exhaustion of the immune system that occurs in such patients.</description><subject>Adult</subject><subject>Arthritis</subject><subject>B-Lymphocyte Subsets - cytology</subject><subject>B-Lymphocyte Subsets - immunology</subject><subject>B-Lymphocyte Subsets - metabolism</subject><subject>B-Lymphocytes, Regulatory - cytology</subject><subject>B-Lymphocytes, Regulatory - immunology</subject><subject>B-Lymphocytes, Regulatory - metabolism</subject><subject>Biology and Life Sciences</subject><subject>Breast cancer</subject><subject>Cardiovascular disease</subject><subject>Case-Control Studies</subject><subject>CD4 antigen</subject><subject>Cell Proliferation</subject><subject>Correlation</subject><subject>Dendritic cells</subject><subject>Exhaustion</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>HIV</subject><subject>HIV - isolation & purification</subject><subject>HIV - physiology</subject><subject>HIV Infections - immunology</subject><subject>HIV Infections - pathology</subject><subject>Homeostasis</subject><subject>Hospitals</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Immune system</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Inflammation</subject><subject>Interleukin 10</subject><subject>Interleukin-10 - metabolism</subject><subject>Laboratories</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Ligands</subject><subject>Lymphocytes</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Middle Aged</subject><subject>Patients</subject><subject>PD-L1 protein</subject><subject>Peripheral blood</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Research and Analysis Methods</subject><subject>Surgery</subject><subject>T-Lymphocytes, Regulatory - cytology</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>T-Lymphocytes, Regulatory - metabolism</subject><subject>Viral Load</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1v1DAQjRCIlsI_QGCJS3vYxY4dJ7kgtRVtV6rEpXC1_DHe9SqxFzsp2j_A78ZL0qpFnDx68-bNzPMUxXuCl4TW5PM2jNHLbrkLHpa4zBhjL4pj0tJywUtMXz6Jj4o3KW0xrmjD-eviiOKW1IS1x8Xv86yxTy6hYNGwAWT2KcJ67OTggkcKhl8AHs1QiHt0gaQ36A5p6LqETi9yakJycIacR5uxlx65vh99MGCdduD1Ht27OGb-zerH2cJ5C3oAg3a5DfghvS1eWdkleDe_J8X3q693lzeL22_Xq8vz24WuSj4sLCO15BWXZckU2KZSjaS0LnnLrGkbYrDRGlvWNqBaxalmoJQpbcMrpZWV9KT4OOnuupDEbGESJWmzVZTwOjNWE8MEuRW76HoZ9yJIJ_4CIa6FjIPTHYiSNq2xhtKDlxwbVUkitQQMmoK0Kmt9mbuNqgej86ZRds9En2e824h1uBecUV6TwzCns0AMP0dIg-hdOhgvPYRxmps0jFdtpn76h_r_7djE0jGk_NH2cRiCxeGsHqrE4azEfFa57MPTRR6LHu6I_gFR6c4F</recordid><startdate>20190327</startdate><enddate>20190327</enddate><creator>Gutiérrez, Carolina</creator><creator>Lopez-Abente, Jacobo</creator><creator>Pérez-Fernández, Verónica</creator><creator>Prieto-Sánchez, Adrián</creator><creator>Correa-Rocha, Rafael</creator><creator>Moreno-Guillen, Santiago</creator><creator>Muñoz-Fernández, María-Ángeles</creator><creator>Pion, Marjorie</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6406-747X</orcidid></search><sort><creationdate>20190327</creationdate><title>Analysis of the dysregulation between regulatory B and T cells (Breg and Treg) in human immunodeficiency virus (HIV)-infected patients</title><author>Gutiérrez, Carolina ; Lopez-Abente, Jacobo ; Pérez-Fernández, Verónica ; Prieto-Sánchez, Adrián ; Correa-Rocha, Rafael ; Moreno-Guillen, Santiago ; Muñoz-Fernández, María-Ángeles ; Pion, Marjorie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-f417a656a224bef85b8a3372694fd981d0dcc0f498eb9b63c4ebbd2f865bcbfa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Arthritis</topic><topic>B-Lymphocyte Subsets - cytology</topic><topic>B-Lymphocyte Subsets - immunology</topic><topic>B-Lymphocyte Subsets - metabolism</topic><topic>B-Lymphocytes, Regulatory - cytology</topic><topic>B-Lymphocytes, Regulatory - immunology</topic><topic>B-Lymphocytes, Regulatory - metabolism</topic><topic>Biology and Life Sciences</topic><topic>Breast cancer</topic><topic>Cardiovascular disease</topic><topic>Case-Control Studies</topic><topic>CD4 antigen</topic><topic>Cell Proliferation</topic><topic>Correlation</topic><topic>Dendritic cells</topic><topic>Exhaustion</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>HIV</topic><topic>HIV - isolation & purification</topic><topic>HIV - physiology</topic><topic>HIV Infections - immunology</topic><topic>HIV Infections - pathology</topic><topic>Homeostasis</topic><topic>Hospitals</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Immune system</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Inflammation</topic><topic>Interleukin 10</topic><topic>Interleukin-10 - metabolism</topic><topic>Laboratories</topic><topic>Leukocytes, Mononuclear - cytology</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Ligands</topic><topic>Lymphocytes</topic><topic>Lymphocytes B</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Middle Aged</topic><topic>Patients</topic><topic>PD-L1 protein</topic><topic>Peripheral blood</topic><topic>Phenotype</topic><topic>Phenotypes</topic><topic>Research and Analysis Methods</topic><topic>Surgery</topic><topic>T-Lymphocytes, Regulatory - cytology</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>T-Lymphocytes, Regulatory - metabolism</topic><topic>Viral Load</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gutiérrez, Carolina</creatorcontrib><creatorcontrib>Lopez-Abente, Jacobo</creatorcontrib><creatorcontrib>Pérez-Fernández, Verónica</creatorcontrib><creatorcontrib>Prieto-Sánchez, Adrián</creatorcontrib><creatorcontrib>Correa-Rocha, Rafael</creatorcontrib><creatorcontrib>Moreno-Guillen, Santiago</creatorcontrib><creatorcontrib>Muñoz-Fernández, María-Ángeles</creatorcontrib><creatorcontrib>Pion, Marjorie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gutiérrez, Carolina</au><au>Lopez-Abente, Jacobo</au><au>Pérez-Fernández, Verónica</au><au>Prieto-Sánchez, Adrián</au><au>Correa-Rocha, Rafael</au><au>Moreno-Guillen, Santiago</au><au>Muñoz-Fernández, María-Ángeles</au><au>Pion, Marjorie</au><au>Kim, Chang H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the dysregulation between regulatory B and T cells (Breg and Treg) in human immunodeficiency virus (HIV)-infected patients</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-03-27</date><risdate>2019</risdate><volume>14</volume><issue>3</issue><spage>e0213744</spage><epage>e0213744</epage><pages>e0213744-e0213744</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This study examines the relationship between regulatory B (Breg) and T (Treg) compartments, which play crucial roles in the maintenance of immune homeostasis in the context of HIV. Using flow cytometry, the phenotypes of different Breg and Treg subsets from HIV-infected and healthy individuals were analyzed, along with the suppressive capacity of Breg. Peripheral blood samples of thirteen HIV+ treatment-naïve individuals, fourteen treated-HIV+ individuals with undetectable viral load and twelve healthy individuals were analyzed. The absolute counts of Breg and Treg subsets were decreased in HIV+ treatment-naïve individuals in comparison to treated-HIV+ and healthy individuals. Interestingly, correlations between Breg subsets (CD24hiCD27+ and PD-L1+ B cells) and IL-10-producing Breg observed in healthy individuals were lost in HIV+ treatment-naïve individuals. However, a correlation between frequencies of CD24hiCD38hi or TIM-1+-Breg subsets and Treg was observed in HIV+ treatment-naïve individuals and not in healthy individuals. Therefore, we hypothesized that various Breg subsets might have different functions during B and T-cell homeostasis during HIV-1 infection. In parallel, stimulated Breg from HIV-infected treatment-naïve individuals presented a decreased ability to suppress CD4+ T-cell proliferation in comparison to the stimulated Breg from treated-HIV+ or healthy individuals. We demonstrate a dysregulation between Breg and Treg subsets in HIV-infected individuals, which might participate in the hyper-activation and exhaustion of the immune system that occurs in such patients.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30917149</pmid><doi>10.1371/journal.pone.0213744</doi><orcidid>https://orcid.org/0000-0002-6406-747X</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_2199323167 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Arthritis B-Lymphocyte Subsets - cytology B-Lymphocyte Subsets - immunology B-Lymphocyte Subsets - metabolism B-Lymphocytes, Regulatory - cytology B-Lymphocytes, Regulatory - immunology B-Lymphocytes, Regulatory - metabolism Biology and Life Sciences Breast cancer Cardiovascular disease Case-Control Studies CD4 antigen Cell Proliferation Correlation Dendritic cells Exhaustion Female Flow cytometry HIV HIV - isolation & purification HIV - physiology HIV Infections - immunology HIV Infections - pathology Homeostasis Hospitals Human immunodeficiency virus Humans Immune system Infections Infectious diseases Inflammation Interleukin 10 Interleukin-10 - metabolism Laboratories Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - metabolism Ligands Lymphocytes Lymphocytes B Lymphocytes T Male Medical research Medicine Medicine and Health Sciences Middle Aged Patients PD-L1 protein Peripheral blood Phenotype Phenotypes Research and Analysis Methods Surgery T-Lymphocytes, Regulatory - cytology T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - metabolism Viral Load Viruses |
| title | Analysis of the dysregulation between regulatory B and T cells (Breg and Treg) in human immunodeficiency virus (HIV)-infected patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T11%3A51%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analysis%20of%20the%20dysregulation%20between%20regulatory%20B%20and%20T%20cells%20(Breg%20and%20Treg)%20in%20human%20immunodeficiency%20virus%20(HIV)-infected%20patients&rft.jtitle=PloS%20one&rft.au=Guti%C3%A9rrez,%20Carolina&rft.date=2019-03-27&rft.volume=14&rft.issue=3&rft.spage=e0213744&rft.epage=e0213744&rft.pages=e0213744-e0213744&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0213744&rft_dat=%3Cproquest_plos_%3E2199323167%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2199323167&rft_id=info:pmid/30917149&rft_doaj_id=oai_doaj_org_article_2389dfd33171460db5a1acae0ec3eafb&rfr_iscdi=true |