Infectious human adenoviruses are shed in urine even after disappearance of urethral symptoms
Urethritis is a common sexually transmitted disease, and human adenoviruses (HAdVs) have been found to be associated with nonchlamydial nongonococcal urethritis. However, the level and viability of HAdV in the urine of patients with urethritis remain unclear. Male patients with urethritis and an asy...
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description | Urethritis is a common sexually transmitted disease, and human adenoviruses (HAdVs) have been found to be associated with nonchlamydial nongonococcal urethritis. However, the level and viability of HAdV in the urine of patients with urethritis remain unclear.
Male patients with urethritis and an asymptomatic group were screened using their First-void urine (FVU) for urethritis-related pathogens to identify those with HAdV DNA. FVU and gargle fluid were collected from all patients including from those in the asymptomatic group. A swab of eye discharge was also collected from patients with eye symptoms. The pharyngeal and/ or ocular fluid was also screened only in cases in which FVU was positive for HAdV DNA. HAdVs were isolated using A549 cell lines and typed by sequencing, and viral shedding during 2 years was quantified using real-time PCR. The prevalence of HAdV was assessed in the urethritis and asymptomatic groups, and viral load, isolated HAdV types, and urethral symptoms were compared between the groups.
The positive detection rate of HAdV DNA was significantly higher in the urethritis group than in the asymptomatic group. Of 398 patients with urethritis, HAdV was isolated in all 32 cases (23 cases in which only HAdV DNA was detected with a mean of 2 × 109 copies/mL in urine samples). Of 124 control cases, one had HAdV monoinfection. The most frequently detected HAdV type was 56, followed by types 37 and 64. Regarding the relationship between symptoms and isolated HAdVs, the virus was isolated for up to 12 days after urethritis symptoms disappeared.
HAdVs were significantly detected and isolated from the FVU of patients with urethritis. Furthermore, high levels of infectious HAdVs are excreted in urine for a long period even after urethritis symptoms disappear. |
doi_str_mv | 10.1371/journal.pone.0212434 |
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Male patients with urethritis and an asymptomatic group were screened using their First-void urine (FVU) for urethritis-related pathogens to identify those with HAdV DNA. FVU and gargle fluid were collected from all patients including from those in the asymptomatic group. A swab of eye discharge was also collected from patients with eye symptoms. The pharyngeal and/ or ocular fluid was also screened only in cases in which FVU was positive for HAdV DNA. HAdVs were isolated using A549 cell lines and typed by sequencing, and viral shedding during 2 years was quantified using real-time PCR. The prevalence of HAdV was assessed in the urethritis and asymptomatic groups, and viral load, isolated HAdV types, and urethral symptoms were compared between the groups.
The positive detection rate of HAdV DNA was significantly higher in the urethritis group than in the asymptomatic group. Of 398 patients with urethritis, HAdV was isolated in all 32 cases (23 cases in which only HAdV DNA was detected with a mean of 2 × 109 copies/mL in urine samples). Of 124 control cases, one had HAdV monoinfection. The most frequently detected HAdV type was 56, followed by types 37 and 64. Regarding the relationship between symptoms and isolated HAdVs, the virus was isolated for up to 12 days after urethritis symptoms disappeared.
HAdVs were significantly detected and isolated from the FVU of patients with urethritis. Furthermore, high levels of infectious HAdVs are excreted in urine for a long period even after urethritis symptoms disappear.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0212434</identifier><identifier>PMID: 30840641</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenovirus Infections, Human - urine ; Adenoviruses ; Adenoviruses, Human ; Adult ; Biology and Life Sciences ; Care and treatment ; Case-Control Studies ; Cell lines ; Control ; Deoxyribonucleic acid ; Disease transmission ; DNA ; DNA sequencing ; Eye ; Eye manifestations ; Gene sequencing ; Genetic aspects ; Humans ; Infectious diseases ; Male ; Medicine and Health Sciences ; Microorganisms ; Non-specific urethritis ; Nongonococcal urethritis ; Pathogenic microorganisms ; Patients ; Pharynx ; Risk factors ; Sexually transmitted diseases ; Sexually Transmitted Diseases - urine ; Sexually Transmitted Diseases - virology ; Signs and symptoms ; STD ; Streptococcus infections ; Studies ; Transplants & implants ; Urethritis ; Urethritis - urine ; Urethritis - virology ; Urine ; Urology ; Viability ; Viral Load ; Viral proteins ; Virus Shedding ; Viruses</subject><ispartof>PloS one, 2019-03, Vol.14 (3), p.e0212434-e0212434</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Hanaoka et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Hanaoka et al 2019 Hanaoka et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-e759fc8ebc50edff99f6b6a860b32c7735b777f6a774845d87486679a1e42f2e3</citedby><cites>FETCH-LOGICAL-c692t-e759fc8ebc50edff99f6b6a860b32c7735b777f6a774845d87486679a1e42f2e3</cites><orcidid>0000-0002-1039-6278 ; 0000-0002-4861-4349</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6402629/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6402629/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30840641$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ulasov, Ilya</contributor><creatorcontrib>Hanaoka, Nozomu</creatorcontrib><creatorcontrib>Ito, Shin</creatorcontrib><creatorcontrib>Konagaya, Masami</creatorcontrib><creatorcontrib>Nojiri, Naomi</creatorcontrib><creatorcontrib>Yasuda, Mitsuru</creatorcontrib><creatorcontrib>Fujimoto, Tsuguto</creatorcontrib><creatorcontrib>Deguchi, Takashi</creatorcontrib><title>Infectious human adenoviruses are shed in urine even after disappearance of urethral symptoms</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Urethritis is a common sexually transmitted disease, and human adenoviruses (HAdVs) have been found to be associated with nonchlamydial nongonococcal urethritis. However, the level and viability of HAdV in the urine of patients with urethritis remain unclear.
Male patients with urethritis and an asymptomatic group were screened using their First-void urine (FVU) for urethritis-related pathogens to identify those with HAdV DNA. FVU and gargle fluid were collected from all patients including from those in the asymptomatic group. A swab of eye discharge was also collected from patients with eye symptoms. The pharyngeal and/ or ocular fluid was also screened only in cases in which FVU was positive for HAdV DNA. HAdVs were isolated using A549 cell lines and typed by sequencing, and viral shedding during 2 years was quantified using real-time PCR. The prevalence of HAdV was assessed in the urethritis and asymptomatic groups, and viral load, isolated HAdV types, and urethral symptoms were compared between the groups.
The positive detection rate of HAdV DNA was significantly higher in the urethritis group than in the asymptomatic group. Of 398 patients with urethritis, HAdV was isolated in all 32 cases (23 cases in which only HAdV DNA was detected with a mean of 2 × 109 copies/mL in urine samples). Of 124 control cases, one had HAdV monoinfection. The most frequently detected HAdV type was 56, followed by types 37 and 64. Regarding the relationship between symptoms and isolated HAdVs, the virus was isolated for up to 12 days after urethritis symptoms disappeared.
HAdVs were significantly detected and isolated from the FVU of patients with urethritis. Furthermore, high levels of infectious HAdVs are excreted in urine for a long period even after urethritis symptoms disappear.</description><subject>Adenovirus Infections, Human - urine</subject><subject>Adenoviruses</subject><subject>Adenoviruses, Human</subject><subject>Adult</subject><subject>Biology and Life Sciences</subject><subject>Care and treatment</subject><subject>Case-Control Studies</subject><subject>Cell lines</subject><subject>Control</subject><subject>Deoxyribonucleic acid</subject><subject>Disease transmission</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>Eye</subject><subject>Eye manifestations</subject><subject>Gene sequencing</subject><subject>Genetic aspects</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Microorganisms</subject><subject>Non-specific urethritis</subject><subject>Nongonococcal urethritis</subject><subject>Pathogenic microorganisms</subject><subject>Patients</subject><subject>Pharynx</subject><subject>Risk factors</subject><subject>Sexually transmitted diseases</subject><subject>Sexually Transmitted Diseases - urine</subject><subject>Sexually Transmitted Diseases - virology</subject><subject>Signs and symptoms</subject><subject>STD</subject><subject>Streptococcus infections</subject><subject>Studies</subject><subject>Transplants & implants</subject><subject>Urethritis</subject><subject>Urethritis - urine</subject><subject>Urethritis - virology</subject><subject>Urine</subject><subject>Urology</subject><subject>Viability</subject><subject>Viral Load</subject><subject>Viral proteins</subject><subject>Virus Shedding</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1uL1DAUx4so7rr6DUQLgujDjLm0afMiLIuXgYUFb28S0uRkJkub1KQd3G9v6nSXqeyD5OGE5Hf-ybll2XOM1phW-N21H4OT7br3DtaIYFLQ4kF2ijklK0YQfXi0P8mexHiNUElrxh5nJxTVBWIFPs1-bpwBNVg_xnw3dtLlUoPzexvGCDGXAfK4A51bl4_BOshhD4kxA4Rc2yj7HmSQTkHuTSJg2AXZ5vGm6wffxafZIyPbCM9me5Z9__jh28Xn1eXVp83F-eVKMU6GFVQlN6qGRpUItDGcG9YwWTPUUKKqipZNVVWGyaoq6qLUdTKMVVxiKIghQM-ylwfdvvVRzJmJguCaY8oJIYnYHAjt5bXog-1kuBFeWvH3wIetkGGwqgUhNatQUxaNUbhoaimV5hqBqaluDGE8ab2fXxubDrQCN6SgF6LLG2d3Yuv3ghWIMDIJvJkFgv81QhxEZ6OCtpUOUiGmf9e8LnDJEvrqH_T-6GZqK1MA1hmf3lWTqDgvU_4QR2jSWt9DpaWhsyq1kbHpfOHwduGQmAF-D1s5xig2X7_8P3v1Y8m-PmJ3INthF307pjZ0cQkWB1AFH2MAc5dkjMQ0BbfZENMUiHkKktuL4wLdOd22Pf0D_3cDVA</recordid><startdate>20190306</startdate><enddate>20190306</enddate><creator>Hanaoka, Nozomu</creator><creator>Ito, Shin</creator><creator>Konagaya, Masami</creator><creator>Nojiri, Naomi</creator><creator>Yasuda, Mitsuru</creator><creator>Fujimoto, Tsuguto</creator><creator>Deguchi, Takashi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1039-6278</orcidid><orcidid>https://orcid.org/0000-0002-4861-4349</orcidid></search><sort><creationdate>20190306</creationdate><title>Infectious human adenoviruses are shed in urine even after disappearance of urethral symptoms</title><author>Hanaoka, Nozomu ; Ito, Shin ; Konagaya, Masami ; Nojiri, Naomi ; Yasuda, Mitsuru ; Fujimoto, Tsuguto ; Deguchi, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-e759fc8ebc50edff99f6b6a860b32c7735b777f6a774845d87486679a1e42f2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adenovirus Infections, Human - urine</topic><topic>Adenoviruses</topic><topic>Adenoviruses, Human</topic><topic>Adult</topic><topic>Biology and Life Sciences</topic><topic>Care and treatment</topic><topic>Case-Control Studies</topic><topic>Cell lines</topic><topic>Control</topic><topic>Deoxyribonucleic acid</topic><topic>Disease transmission</topic><topic>DNA</topic><topic>DNA sequencing</topic><topic>Eye</topic><topic>Eye manifestations</topic><topic>Gene sequencing</topic><topic>Genetic aspects</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medicine and Health Sciences</topic><topic>Microorganisms</topic><topic>Non-specific urethritis</topic><topic>Nongonococcal urethritis</topic><topic>Pathogenic microorganisms</topic><topic>Patients</topic><topic>Pharynx</topic><topic>Risk factors</topic><topic>Sexually transmitted diseases</topic><topic>Sexually Transmitted Diseases - urine</topic><topic>Sexually Transmitted Diseases - virology</topic><topic>Signs and symptoms</topic><topic>STD</topic><topic>Streptococcus infections</topic><topic>Studies</topic><topic>Transplants & implants</topic><topic>Urethritis</topic><topic>Urethritis - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hanaoka, Nozomu</au><au>Ito, Shin</au><au>Konagaya, Masami</au><au>Nojiri, Naomi</au><au>Yasuda, Mitsuru</au><au>Fujimoto, Tsuguto</au><au>Deguchi, Takashi</au><au>Ulasov, Ilya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Infectious human adenoviruses are shed in urine even after disappearance of urethral symptoms</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-03-06</date><risdate>2019</risdate><volume>14</volume><issue>3</issue><spage>e0212434</spage><epage>e0212434</epage><pages>e0212434-e0212434</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Urethritis is a common sexually transmitted disease, and human adenoviruses (HAdVs) have been found to be associated with nonchlamydial nongonococcal urethritis. However, the level and viability of HAdV in the urine of patients with urethritis remain unclear.
Male patients with urethritis and an asymptomatic group were screened using their First-void urine (FVU) for urethritis-related pathogens to identify those with HAdV DNA. FVU and gargle fluid were collected from all patients including from those in the asymptomatic group. A swab of eye discharge was also collected from patients with eye symptoms. The pharyngeal and/ or ocular fluid was also screened only in cases in which FVU was positive for HAdV DNA. HAdVs were isolated using A549 cell lines and typed by sequencing, and viral shedding during 2 years was quantified using real-time PCR. The prevalence of HAdV was assessed in the urethritis and asymptomatic groups, and viral load, isolated HAdV types, and urethral symptoms were compared between the groups.
The positive detection rate of HAdV DNA was significantly higher in the urethritis group than in the asymptomatic group. Of 398 patients with urethritis, HAdV was isolated in all 32 cases (23 cases in which only HAdV DNA was detected with a mean of 2 × 109 copies/mL in urine samples). Of 124 control cases, one had HAdV monoinfection. The most frequently detected HAdV type was 56, followed by types 37 and 64. Regarding the relationship between symptoms and isolated HAdVs, the virus was isolated for up to 12 days after urethritis symptoms disappeared.
HAdVs were significantly detected and isolated from the FVU of patients with urethritis. Furthermore, high levels of infectious HAdVs are excreted in urine for a long period even after urethritis symptoms disappear.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30840641</pmid><doi>10.1371/journal.pone.0212434</doi><tpages>e0212434</tpages><orcidid>https://orcid.org/0000-0002-1039-6278</orcidid><orcidid>https://orcid.org/0000-0002-4861-4349</orcidid><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
subjects | Adenovirus Infections, Human - urine Adenoviruses Adenoviruses, Human Adult Biology and Life Sciences Care and treatment Case-Control Studies Cell lines Control Deoxyribonucleic acid Disease transmission DNA DNA sequencing Eye Eye manifestations Gene sequencing Genetic aspects Humans Infectious diseases Male Medicine and Health Sciences Microorganisms Non-specific urethritis Nongonococcal urethritis Pathogenic microorganisms Patients Pharynx Risk factors Sexually transmitted diseases Sexually Transmitted Diseases - urine Sexually Transmitted Diseases - virology Signs and symptoms STD Streptococcus infections Studies Transplants & implants Urethritis Urethritis - urine Urethritis - virology Urine Urology Viability Viral Load Viral proteins Virus Shedding Viruses |
title | Infectious human adenoviruses are shed in urine even after disappearance of urethral symptoms |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T14%3A49%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Infectious%20human%20adenoviruses%20are%20shed%20in%20urine%20even%20after%20disappearance%20of%20urethral%20symptoms&rft.jtitle=PloS%20one&rft.au=Hanaoka,%20Nozomu&rft.date=2019-03-06&rft.volume=14&rft.issue=3&rft.spage=e0212434&rft.epage=e0212434&rft.pages=e0212434-e0212434&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0212434&rft_dat=%3Cgale_plos_%3EA577309006%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2189139222&rft_id=info:pmid/30840641&rft_galeid=A577309006&rft_doaj_id=oai_doaj_org_article_ad670b54bfc14b8aacd9d0ef83dbf269&rfr_iscdi=true |