Rapid and sensitive detection of NADPH via mBFP-mediated enhancement of its fluorescence

The reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) functions as a reducing agent involved in many biosynthetic and antioxidant reactions in cells. Therefore, a lots of detection or assaying method of this cofactor are developed and used broadly in various research and applicatio...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2019-02, Vol.14 (2), p.e0212061-e0212061
Hauptverfasser:	You, Sung-Hwan, Lim, Ho-Dong, Cheong, Dae-Eun, Kim, Eung-Sam, Kim, Geun-Joong
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Antioxidants Antioxidants (Nutrients) Assaying Biology and Life Sciences Biomarkers Cancer Catalysis Cells (Biology) Cells, Cultured Cofactors Cytochrome Dehydrogenases Enzymatic activity Enzymes Fluorescence Fluorescent proteins Glucosephosphate Dehydrogenase - metabolism Humans Luminescent Measurements - methods Luminescent Proteins - chemistry Luminescent Proteins - genetics Luminescent Proteins - metabolism Lysates Metabolism Metabolites Metagenome Methods NADH NADP NADP - analysis NADP - metabolism NADPH-diaphorase Niacinamide Nicotinamide Nicotinamide adenine dinucleotide Nicotinamide adenine dinucleotide phosphate Nicotine Oxidation-Reduction Phosphates Phosphotransferases - metabolism Physical Sciences Polymer antioxidants (Additives) Proteins Purines Reducing agents Research and Analysis Methods Sensitivity and Specificity Time Factors
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!