The effect of endurance training and testosterone supplementation on the expression of blood spinal cord barrier proteins in rats
The present study aimed to estimate the effect of endurance training, two doses of testosterone, and the combination of these stimuli on the level of the endothelial proteins claudin, occludin, JAM-1, VE-cadherin, ZO-1, ZO-2, and P-glycoprotein in rat spinal cords. Adult male Wistar rats were traine...
Gespeichert in:
| Veröffentlicht in: | PloS one 2019-02, Vol.14 (2), p.e0211818-e0211818 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0211818 |
|---|---|
| container_issue | 2 |
| container_start_page | e0211818 |
| container_title | PloS one |
| container_volume | 14 |
| creator | Nierwińska, Katarzyna Nowacka-Chmielewska, Marta Bernacki, Jacek Jagsz, Sławomir Chalimoniuk, Małgorzata Langfort, Józef Małecki, Andrzej |
| description | The present study aimed to estimate the effect of endurance training, two doses of testosterone, and the combination of these stimuli on the level of the endothelial proteins claudin, occludin, JAM-1, VE-cadherin, ZO-1, ZO-2, and P-glycoprotein in rat spinal cords. Adult male Wistar rats were trained using a motor-driven treadmill for 6 weeks (40-60 min, 5 times per week) and/or were treated for 6 weeks with two doses of testosterone (i.m.; 8 mg/kg or 80 mg/kg body weight). Spinal cords were collected 48 hours after the last training cycle and stored at -80°C. The levels of selected proteins in whole tissue lysates of the spinal cord were measured by western blot. Testosterone-treated trained rats had significantly lower claudin levels than vehicle-treated trained rats. High doses of testosterone resulted in a significant decrease in claudin-5 in untrained rats compared to the control group. Both doses of testosterone significantly reduced occludin levels compared to those in vehicle-treated untrained rats. The JAM-1 level in the spinal cords of both trained and untrained animals receiving testosterone was decreased in a dose-dependent manner. The JAM-1 level in the trained group treated with high doses of testosterone was significantly higher than that in the untrained rats treated with 80 mg/kg of testosterone. VE-cadherin levels were decreased in all groups receiving testosterone regardless of endurance training and were also diminished in the vehicle-treated group compared to the control group. Testosterone treatment did not exert a significant effect on ZO-1 protein levels. Testosterone and/or training had no significant effects on ZO-2 protein levels in the rat spinal cords. Endurance training increased P-glycoprotein levels in the rat spinal cords. The results suggest that an excessive supply of testosterone may adversely impact the expression of endothelial proteins in the central nervous system, which, in turn, may affect the blood-brain barrier function. |
| doi_str_mv | 10.1371/journal.pone.0211818 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2178592352</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A573659870</galeid><doaj_id>oai_doaj_org_article_e2a6f6cea6d24237a2f15140a64889b5</doaj_id><sourcerecordid>A573659870</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-81870a8a291c2a569d02cf865c081a27e3e943e1694011e0eaacad7853c8b7553</originalsourceid><addsrcrecordid>eNqNk01r3DAQhk1padJt_0FpBYXSHnarD0u2L4UQ-hEIBNq0VzErj3e1eCVHkkt67D-vnGzCbsmhYLAZPfOO5h1PUbxkdMFExT5s_Bgc9IvBO1xQzljN6kfFMWsEnytOxeO976PiWYwbSqWolXpaHAlalVzJ-rj4c7lGgl2HJhHfEXTtGMAZJCmAddatCLiWJIzJx4Qh1yJxHIYet-gSJOsdyU-aRK6HgDHeRDqy7L1vSRxsviIxPrRkCSFYDGQIPqF1kVhHAqT4vHjSQR_xxe49K358_nR5-nV-fvHl7PTkfG5Uw9M8d1dRqIE3zHCQqmkpN12tpKE1A16hwKYUyFRTUsaQIoCBtqqlMPWyklLMite3ukPvo965FzVnmWm4kDwTZ7dE62Gjh2C3EH5rD1bfBHxYaQjJmh41clCdMgiq5SUXFfCOSVZSUGVdN8up2sddtXG5xdZktwL0B6KHJ86u9cr_0kpUlOVOZsW7nUDwV2P2X29tNNj34NCP071rKWU1TXdWvPkHfbi7HbWC3IB1nc91zSSqT2QllGyywZlaPEBNVuLWmjz-zub4QcL7g4TMJLxOKxhj1Gffv_0_e_HzkH27x64R-rSOvh-nXy4eguUtaIKPMWB3bzKjetqUOzf0tCl6tyk57dX-gO6T7lZD_AVWFg-I</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2178592352</pqid></control><display><type>article</type><title>The effect of endurance training and testosterone supplementation on the expression of blood spinal cord barrier proteins in rats</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Nierwińska, Katarzyna ; Nowacka-Chmielewska, Marta ; Bernacki, Jacek ; Jagsz, Sławomir ; Chalimoniuk, Małgorzata ; Langfort, Józef ; Małecki, Andrzej</creator><contributor>Toborek, Michal</contributor><creatorcontrib>Nierwińska, Katarzyna ; Nowacka-Chmielewska, Marta ; Bernacki, Jacek ; Jagsz, Sławomir ; Chalimoniuk, Małgorzata ; Langfort, Józef ; Małecki, Andrzej ; Toborek, Michal</creatorcontrib><description>The present study aimed to estimate the effect of endurance training, two doses of testosterone, and the combination of these stimuli on the level of the endothelial proteins claudin, occludin, JAM-1, VE-cadherin, ZO-1, ZO-2, and P-glycoprotein in rat spinal cords. Adult male Wistar rats were trained using a motor-driven treadmill for 6 weeks (40-60 min, 5 times per week) and/or were treated for 6 weeks with two doses of testosterone (i.m.; 8 mg/kg or 80 mg/kg body weight). Spinal cords were collected 48 hours after the last training cycle and stored at -80°C. The levels of selected proteins in whole tissue lysates of the spinal cord were measured by western blot. Testosterone-treated trained rats had significantly lower claudin levels than vehicle-treated trained rats. High doses of testosterone resulted in a significant decrease in claudin-5 in untrained rats compared to the control group. Both doses of testosterone significantly reduced occludin levels compared to those in vehicle-treated untrained rats. The JAM-1 level in the spinal cords of both trained and untrained animals receiving testosterone was decreased in a dose-dependent manner. The JAM-1 level in the trained group treated with high doses of testosterone was significantly higher than that in the untrained rats treated with 80 mg/kg of testosterone. VE-cadherin levels were decreased in all groups receiving testosterone regardless of endurance training and were also diminished in the vehicle-treated group compared to the control group. Testosterone treatment did not exert a significant effect on ZO-1 protein levels. Testosterone and/or training had no significant effects on ZO-2 protein levels in the rat spinal cords. Endurance training increased P-glycoprotein levels in the rat spinal cords. The results suggest that an excessive supply of testosterone may adversely impact the expression of endothelial proteins in the central nervous system, which, in turn, may affect the blood-brain barrier function.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0211818</identifier><identifier>PMID: 30742658</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aerobics ; Analysis ; Androgens ; Animals ; Antigens, CD - biosynthesis ; Atherosclerosis ; ATP Binding Cassette Transporter, Subfamily B, Member 1 - biosynthesis ; Biology and Life Sciences ; Blood-brain barrier ; Body weight ; Cadherins ; Cadherins - biosynthesis ; Cell Adhesion Molecules - biosynthesis ; Central nervous system ; Claudin-5 - biosynthesis ; Dosage and administration ; Dose-Response Relationship, Drug ; Drug dosages ; Endothelium ; Endothelium, Vascular - metabolism ; Endurance ; Exercise ; Exercise equipment ; Fatigue tests ; Fuel consumption ; Gene expression ; Gene Expression Regulation - drug effects ; Glycoproteins ; Health aspects ; Hormones ; Hypoxia ; Influence ; Laboratories ; Lysates ; Male ; Medicine and Health Sciences ; Membrane proteins ; Methamphetamine ; Molecular biology ; Oxidative stress ; P-Glycoprotein ; Permeability ; Physical Conditioning, Animal ; Physical education ; Physical Endurance - drug effects ; Physical Sciences ; Physical training ; Physiology ; Proteins ; Rats ; Rats, Wistar ; Research and Analysis Methods ; Shear stress ; Spinal cord ; Spinal Cord - chemistry ; Spinal Cord - metabolism ; Supplements ; Testosterone ; Testosterone - pharmacology ; Training ; Zonula occludens-1 protein ; Zonula Occludens-1 Protein - biosynthesis ; Zonula Occludens-2 Protein - biosynthesis</subject><ispartof>PloS one, 2019-02, Vol.14 (2), p.e0211818-e0211818</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Nierwińska et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Nierwińska et al 2019 Nierwińska et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-81870a8a291c2a569d02cf865c081a27e3e943e1694011e0eaacad7853c8b7553</citedby><cites>FETCH-LOGICAL-c692t-81870a8a291c2a569d02cf865c081a27e3e943e1694011e0eaacad7853c8b7553</cites><orcidid>0000-0001-8668-8878 ; 0000-0003-2958-783X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370194/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370194/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30742658$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Toborek, Michal</contributor><creatorcontrib>Nierwińska, Katarzyna</creatorcontrib><creatorcontrib>Nowacka-Chmielewska, Marta</creatorcontrib><creatorcontrib>Bernacki, Jacek</creatorcontrib><creatorcontrib>Jagsz, Sławomir</creatorcontrib><creatorcontrib>Chalimoniuk, Małgorzata</creatorcontrib><creatorcontrib>Langfort, Józef</creatorcontrib><creatorcontrib>Małecki, Andrzej</creatorcontrib><title>The effect of endurance training and testosterone supplementation on the expression of blood spinal cord barrier proteins in rats</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The present study aimed to estimate the effect of endurance training, two doses of testosterone, and the combination of these stimuli on the level of the endothelial proteins claudin, occludin, JAM-1, VE-cadherin, ZO-1, ZO-2, and P-glycoprotein in rat spinal cords. Adult male Wistar rats were trained using a motor-driven treadmill for 6 weeks (40-60 min, 5 times per week) and/or were treated for 6 weeks with two doses of testosterone (i.m.; 8 mg/kg or 80 mg/kg body weight). Spinal cords were collected 48 hours after the last training cycle and stored at -80°C. The levels of selected proteins in whole tissue lysates of the spinal cord were measured by western blot. Testosterone-treated trained rats had significantly lower claudin levels than vehicle-treated trained rats. High doses of testosterone resulted in a significant decrease in claudin-5 in untrained rats compared to the control group. Both doses of testosterone significantly reduced occludin levels compared to those in vehicle-treated untrained rats. The JAM-1 level in the spinal cords of both trained and untrained animals receiving testosterone was decreased in a dose-dependent manner. The JAM-1 level in the trained group treated with high doses of testosterone was significantly higher than that in the untrained rats treated with 80 mg/kg of testosterone. VE-cadherin levels were decreased in all groups receiving testosterone regardless of endurance training and were also diminished in the vehicle-treated group compared to the control group. Testosterone treatment did not exert a significant effect on ZO-1 protein levels. Testosterone and/or training had no significant effects on ZO-2 protein levels in the rat spinal cords. Endurance training increased P-glycoprotein levels in the rat spinal cords. The results suggest that an excessive supply of testosterone may adversely impact the expression of endothelial proteins in the central nervous system, which, in turn, may affect the blood-brain barrier function.</description><subject>Aerobics</subject><subject>Analysis</subject><subject>Androgens</subject><subject>Animals</subject><subject>Antigens, CD - biosynthesis</subject><subject>Atherosclerosis</subject><subject>ATP Binding Cassette Transporter, Subfamily B, Member 1 - biosynthesis</subject><subject>Biology and Life Sciences</subject><subject>Blood-brain barrier</subject><subject>Body weight</subject><subject>Cadherins</subject><subject>Cadherins - biosynthesis</subject><subject>Cell Adhesion Molecules - biosynthesis</subject><subject>Central nervous system</subject><subject>Claudin-5 - biosynthesis</subject><subject>Dosage and administration</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug dosages</subject><subject>Endothelium</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Endurance</subject><subject>Exercise</subject><subject>Exercise equipment</subject><subject>Fatigue tests</subject><subject>Fuel consumption</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Glycoproteins</subject><subject>Health aspects</subject><subject>Hormones</subject><subject>Hypoxia</subject><subject>Influence</subject><subject>Laboratories</subject><subject>Lysates</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Membrane proteins</subject><subject>Methamphetamine</subject><subject>Molecular biology</subject><subject>Oxidative stress</subject><subject>P-Glycoprotein</subject><subject>Permeability</subject><subject>Physical Conditioning, Animal</subject><subject>Physical education</subject><subject>Physical Endurance - drug effects</subject><subject>Physical Sciences</subject><subject>Physical training</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Research and Analysis Methods</subject><subject>Shear stress</subject><subject>Spinal cord</subject><subject>Spinal Cord - chemistry</subject><subject>Spinal Cord - metabolism</subject><subject>Supplements</subject><subject>Testosterone</subject><subject>Testosterone - pharmacology</subject><subject>Training</subject><subject>Zonula occludens-1 protein</subject><subject>Zonula Occludens-1 Protein - biosynthesis</subject><subject>Zonula Occludens-2 Protein - biosynthesis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01r3DAQhk1padJt_0FpBYXSHnarD0u2L4UQ-hEIBNq0VzErj3e1eCVHkkt67D-vnGzCbsmhYLAZPfOO5h1PUbxkdMFExT5s_Bgc9IvBO1xQzljN6kfFMWsEnytOxeO976PiWYwbSqWolXpaHAlalVzJ-rj4c7lGgl2HJhHfEXTtGMAZJCmAddatCLiWJIzJx4Qh1yJxHIYet-gSJOsdyU-aRK6HgDHeRDqy7L1vSRxsviIxPrRkCSFYDGQIPqF1kVhHAqT4vHjSQR_xxe49K358_nR5-nV-fvHl7PTkfG5Uw9M8d1dRqIE3zHCQqmkpN12tpKE1A16hwKYUyFRTUsaQIoCBtqqlMPWyklLMite3ukPvo965FzVnmWm4kDwTZ7dE62Gjh2C3EH5rD1bfBHxYaQjJmh41clCdMgiq5SUXFfCOSVZSUGVdN8up2sddtXG5xdZktwL0B6KHJ86u9cr_0kpUlOVOZsW7nUDwV2P2X29tNNj34NCP071rKWU1TXdWvPkHfbi7HbWC3IB1nc91zSSqT2QllGyywZlaPEBNVuLWmjz-zub4QcL7g4TMJLxOKxhj1Gffv_0_e_HzkH27x64R-rSOvh-nXy4eguUtaIKPMWB3bzKjetqUOzf0tCl6tyk57dX-gO6T7lZD_AVWFg-I</recordid><startdate>20190211</startdate><enddate>20190211</enddate><creator>Nierwińska, Katarzyna</creator><creator>Nowacka-Chmielewska, Marta</creator><creator>Bernacki, Jacek</creator><creator>Jagsz, Sławomir</creator><creator>Chalimoniuk, Małgorzata</creator><creator>Langfort, Józef</creator><creator>Małecki, Andrzej</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-8668-8878</orcidid><orcidid>https://orcid.org/0000-0003-2958-783X</orcidid></search><sort><creationdate>20190211</creationdate><title>The effect of endurance training and testosterone supplementation on the expression of blood spinal cord barrier proteins in rats</title><author>Nierwińska, Katarzyna ; Nowacka-Chmielewska, Marta ; Bernacki, Jacek ; Jagsz, Sławomir ; Chalimoniuk, Małgorzata ; Langfort, Józef ; Małecki, Andrzej</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-81870a8a291c2a569d02cf865c081a27e3e943e1694011e0eaacad7853c8b7553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aerobics</topic><topic>Analysis</topic><topic>Androgens</topic><topic>Animals</topic><topic>Antigens, CD - biosynthesis</topic><topic>Atherosclerosis</topic><topic>ATP Binding Cassette Transporter, Subfamily B, Member 1 - biosynthesis</topic><topic>Biology and Life Sciences</topic><topic>Blood-brain barrier</topic><topic>Body weight</topic><topic>Cadherins</topic><topic>Cadherins - biosynthesis</topic><topic>Cell Adhesion Molecules - biosynthesis</topic><topic>Central nervous system</topic><topic>Claudin-5 - biosynthesis</topic><topic>Dosage and administration</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug dosages</topic><topic>Endothelium</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Endurance</topic><topic>Exercise</topic><topic>Exercise equipment</topic><topic>Fatigue tests</topic><topic>Fuel consumption</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Glycoproteins</topic><topic>Health aspects</topic><topic>Hormones</topic><topic>Hypoxia</topic><topic>Influence</topic><topic>Laboratories</topic><topic>Lysates</topic><topic>Male</topic><topic>Medicine and Health Sciences</topic><topic>Membrane proteins</topic><topic>Methamphetamine</topic><topic>Molecular biology</topic><topic>Oxidative stress</topic><topic>P-Glycoprotein</topic><topic>Permeability</topic><topic>Physical Conditioning, Animal</topic><topic>Physical education</topic><topic>Physical Endurance - drug effects</topic><topic>Physical Sciences</topic><topic>Physical training</topic><topic>Physiology</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Research and Analysis Methods</topic><topic>Shear stress</topic><topic>Spinal cord</topic><topic>Spinal Cord - chemistry</topic><topic>Spinal Cord - metabolism</topic><topic>Supplements</topic><topic>Testosterone</topic><topic>Testosterone - pharmacology</topic><topic>Training</topic><topic>Zonula occludens-1 protein</topic><topic>Zonula Occludens-1 Protein - biosynthesis</topic><topic>Zonula Occludens-2 Protein - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nierwińska, Katarzyna</creatorcontrib><creatorcontrib>Nowacka-Chmielewska, Marta</creatorcontrib><creatorcontrib>Bernacki, Jacek</creatorcontrib><creatorcontrib>Jagsz, Sławomir</creatorcontrib><creatorcontrib>Chalimoniuk, Małgorzata</creatorcontrib><creatorcontrib>Langfort, Józef</creatorcontrib><creatorcontrib>Małecki, Andrzej</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nierwińska, Katarzyna</au><au>Nowacka-Chmielewska, Marta</au><au>Bernacki, Jacek</au><au>Jagsz, Sławomir</au><au>Chalimoniuk, Małgorzata</au><au>Langfort, Józef</au><au>Małecki, Andrzej</au><au>Toborek, Michal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of endurance training and testosterone supplementation on the expression of blood spinal cord barrier proteins in rats</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-02-11</date><risdate>2019</risdate><volume>14</volume><issue>2</issue><spage>e0211818</spage><epage>e0211818</epage><pages>e0211818-e0211818</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The present study aimed to estimate the effect of endurance training, two doses of testosterone, and the combination of these stimuli on the level of the endothelial proteins claudin, occludin, JAM-1, VE-cadherin, ZO-1, ZO-2, and P-glycoprotein in rat spinal cords. Adult male Wistar rats were trained using a motor-driven treadmill for 6 weeks (40-60 min, 5 times per week) and/or were treated for 6 weeks with two doses of testosterone (i.m.; 8 mg/kg or 80 mg/kg body weight). Spinal cords were collected 48 hours after the last training cycle and stored at -80°C. The levels of selected proteins in whole tissue lysates of the spinal cord were measured by western blot. Testosterone-treated trained rats had significantly lower claudin levels than vehicle-treated trained rats. High doses of testosterone resulted in a significant decrease in claudin-5 in untrained rats compared to the control group. Both doses of testosterone significantly reduced occludin levels compared to those in vehicle-treated untrained rats. The JAM-1 level in the spinal cords of both trained and untrained animals receiving testosterone was decreased in a dose-dependent manner. The JAM-1 level in the trained group treated with high doses of testosterone was significantly higher than that in the untrained rats treated with 80 mg/kg of testosterone. VE-cadherin levels were decreased in all groups receiving testosterone regardless of endurance training and were also diminished in the vehicle-treated group compared to the control group. Testosterone treatment did not exert a significant effect on ZO-1 protein levels. Testosterone and/or training had no significant effects on ZO-2 protein levels in the rat spinal cords. Endurance training increased P-glycoprotein levels in the rat spinal cords. The results suggest that an excessive supply of testosterone may adversely impact the expression of endothelial proteins in the central nervous system, which, in turn, may affect the blood-brain barrier function.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30742658</pmid><doi>10.1371/journal.pone.0211818</doi><tpages>e0211818</tpages><orcidid>https://orcid.org/0000-0001-8668-8878</orcidid><orcidid>https://orcid.org/0000-0003-2958-783X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-02, Vol.14 (2), p.e0211818-e0211818 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2178592352 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aerobics Analysis Androgens Animals Antigens, CD - biosynthesis Atherosclerosis ATP Binding Cassette Transporter, Subfamily B, Member 1 - biosynthesis Biology and Life Sciences Blood-brain barrier Body weight Cadherins Cadherins - biosynthesis Cell Adhesion Molecules - biosynthesis Central nervous system Claudin-5 - biosynthesis Dosage and administration Dose-Response Relationship, Drug Drug dosages Endothelium Endothelium, Vascular - metabolism Endurance Exercise Exercise equipment Fatigue tests Fuel consumption Gene expression Gene Expression Regulation - drug effects Glycoproteins Health aspects Hormones Hypoxia Influence Laboratories Lysates Male Medicine and Health Sciences Membrane proteins Methamphetamine Molecular biology Oxidative stress P-Glycoprotein Permeability Physical Conditioning, Animal Physical education Physical Endurance - drug effects Physical Sciences Physical training Physiology Proteins Rats Rats, Wistar Research and Analysis Methods Shear stress Spinal cord Spinal Cord - chemistry Spinal Cord - metabolism Supplements Testosterone Testosterone - pharmacology Training Zonula occludens-1 protein Zonula Occludens-1 Protein - biosynthesis Zonula Occludens-2 Protein - biosynthesis |
| title | The effect of endurance training and testosterone supplementation on the expression of blood spinal cord barrier proteins in rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T21%3A09%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20effect%20of%20endurance%20training%20and%20testosterone%20supplementation%20on%20the%20expression%20of%20blood%20spinal%20cord%20barrier%20proteins%20in%20rats&rft.jtitle=PloS%20one&rft.au=Nierwi%C5%84ska,%20Katarzyna&rft.date=2019-02-11&rft.volume=14&rft.issue=2&rft.spage=e0211818&rft.epage=e0211818&rft.pages=e0211818-e0211818&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0211818&rft_dat=%3Cgale_plos_%3EA573659870%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2178592352&rft_id=info:pmid/30742658&rft_galeid=A573659870&rft_doaj_id=oai_doaj_org_article_e2a6f6cea6d24237a2f15140a64889b5&rfr_iscdi=true |