Thermotolerant isolates of Beauveria bassiana as potential control agent of insect pest in subtropical climates
The use of Beauveria bassiana in biological control of agricultural pests is mainly hampered by environmental factors, such as elevated temperatures and low humidity. These limitations, further amplified in a global warming scenario, could nullify biological control strategies based on this fungus....
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| description | The use of Beauveria bassiana in biological control of agricultural pests is mainly hampered by environmental factors, such as elevated temperatures and low humidity. These limitations, further amplified in a global warming scenario, could nullify biological control strategies based on this fungus. The identification of thermotolerant B. bassiana isolates represents a possible strategy to overcome this problem. In this study, in order to maximize the probability in the isolation of thermotolerant B. bassiana, soil samples and infected insects were collected in warm areas of Syria. The obtained fungal isolates were tested for different biological parameters (i.e., growth rate, sporulation and spore germination) at growing temperatures ranging from 20°C to 35°C. Among these isolates (eight from insects and 11 from soil samples), the five with the highest growth rate, spore production and germination at 30°C were tested for their entomopathogenicity through in vivo assays on Ephestia kuehniella larvae. Insect mortality induced by the five isolates ranged from 31% to 100%. Two isolates, one from Phyllognathus excavatus and one from soil, caused 50% of the larval mortality in less than four days, reaching values exceeding 92% in ten days. These two isolates were molecularly identified as B. bassiana sensu stricto by using three markers (i.e., ITS, Bloc and EF1-α). Considering these promising results, further studies are ongoing, testing their efficiency in field conditions as control agents for agricultural insect pests in Mediterranean and Subtropical regions. |
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These limitations, further amplified in a global warming scenario, could nullify biological control strategies based on this fungus. The identification of thermotolerant B. bassiana isolates represents a possible strategy to overcome this problem. In this study, in order to maximize the probability in the isolation of thermotolerant B. bassiana, soil samples and infected insects were collected in warm areas of Syria. The obtained fungal isolates were tested for different biological parameters (i.e., growth rate, sporulation and spore germination) at growing temperatures ranging from 20°C to 35°C. Among these isolates (eight from insects and 11 from soil samples), the five with the highest growth rate, spore production and germination at 30°C were tested for their entomopathogenicity through in vivo assays on Ephestia kuehniella larvae. Insect mortality induced by the five isolates ranged from 31% to 100%. Two isolates, one from Phyllognathus excavatus and one from soil, caused 50% of the larval mortality in less than four days, reaching values exceeding 92% in ten days. These two isolates were molecularly identified as B. bassiana sensu stricto by using three markers (i.e., ITS, Bloc and EF1-α). Considering these promising results, further studies are ongoing, testing their efficiency in field conditions as control agents for agricultural insect pests in Mediterranean and Subtropical regions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0211457</identifier><identifier>PMID: 30707750</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agricultural pests ; Agricultural production ; Animals ; Ascomycota ; Beauveria - classification ; Beauveria - genetics ; Beauveria - isolation & purification ; Beauveria - physiology ; Beauveria bassiana ; Biological control ; Biological pest control ; Biology and Life Sciences ; Biopesticides ; Climate ; Climate change ; Control ; Environmental factors ; Fungal Proteins - genetics ; Fungi ; Germination ; Global temperature changes ; Global warming ; Growth rate ; High temperature ; Humidity ; In vivo methods and tests ; Insect pests ; Insecta - growth & development ; Insecta - microbiology ; Insects ; Larva - microbiology ; Larvae ; Low humidity ; Mitochondrial DNA ; Morphology ; Mortality ; Peptide Elongation Factor 1 - genetics ; Pest control ; Pest Control, Biological - methods ; Pests ; Phylogenetics ; Phylogeny ; Plant diseases ; Research and Analysis Methods ; Soil Microbiology ; Soils ; Spore germination ; Sporulation ; Subtropical climates ; Syria ; Temperature effects ; Thermotolerance ; Tropical Climate</subject><ispartof>PloS one, 2019-02, Vol.14 (2), p.e0211457</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Alali et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Considering these promising results, further studies are ongoing, testing their efficiency in field conditions as control agents for agricultural insect pests in Mediterranean and Subtropical regions.</description><subject>Agricultural pests</subject><subject>Agricultural production</subject><subject>Animals</subject><subject>Ascomycota</subject><subject>Beauveria - classification</subject><subject>Beauveria - genetics</subject><subject>Beauveria - isolation & purification</subject><subject>Beauveria - physiology</subject><subject>Beauveria bassiana</subject><subject>Biological control</subject><subject>Biological pest control</subject><subject>Biology and Life Sciences</subject><subject>Biopesticides</subject><subject>Climate</subject><subject>Climate change</subject><subject>Control</subject><subject>Environmental factors</subject><subject>Fungal Proteins - genetics</subject><subject>Fungi</subject><subject>Germination</subject><subject>Global temperature changes</subject><subject>Global warming</subject><subject>Growth rate</subject><subject>High temperature</subject><subject>Humidity</subject><subject>In vivo methods and tests</subject><subject>Insect pests</subject><subject>Insecta - growth & development</subject><subject>Insecta - microbiology</subject><subject>Insects</subject><subject>Larva - microbiology</subject><subject>Larvae</subject><subject>Low humidity</subject><subject>Mitochondrial DNA</subject><subject>Morphology</subject><subject>Mortality</subject><subject>Peptide Elongation Factor 1 - genetics</subject><subject>Pest control</subject><subject>Pest Control, Biological - methods</subject><subject>Pests</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant diseases</subject><subject>Research and Analysis Methods</subject><subject>Soil Microbiology</subject><subject>Soils</subject><subject>Spore germination</subject><subject>Sporulation</subject><subject>Subtropical climates</subject><subject>Syria</subject><subject>Temperature effects</subject><subject>Thermotolerance</subject><subject>Tropical Climate</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01v1DAQhiMEoqXwDxBEQkJw2MV2Yju-IJWKj5UqVYLC1Zo4zq4rr721nQr-PU43rTaoB5SDo_Hzvp4Ze4riJUZLXHH84coPwYFd7rzTS0Qwril_VBxjUZEFI6h6fPB_VDyL8QohWjWMPS2OKsQR5xQdF_5yo8PWJ291AJdKE72FpGPp-_KThuFGBwNlCzEacFBCLHc-aZcM2FJ5l4K3JaxzYBQYF7VK5U7HbOTKOLR5f2fUyFqzHX2fF096sFG_mNaT4ueXz5dn3xbnF19XZ6fnC8UESQvVdaIXlCgioOFUgep60XaY4ipHhegYbUXTcwQE1bqmDWdN2zDSsgY4Q1CdFK_3vjvro5x6FSXBvOY1wYJmYrUnOg9XchdyfuGP9GDkbcCHtYSQjLJaoo5TxhlpBIVaqQqYgFqzRquuVT3n2evjdNrQbnWncj8C2JnpfMeZjVz7G8kq2mBaZ4N3k0Hw10Pun9yaqLS14LQfbvMWtSBNPeb95h_04eomag25AON6n89Vo6k8pZwgnuupMrV8gMpfp7cmX6_uTY7PBO9ngvEJ6N9pDUOMcvXj-_-zF7_m7NsDdqPBpk1-iUMy3sU5WO9BFXyMQff3TcZIjnNx1w05zoWc5iLLXh1e0L3obhCqvyLTCXY</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Alali, Sumer</creator><creator>Mereghetti, Valeria</creator><creator>Faoro, Franco</creator><creator>Bocchi, Stefano</creator><creator>Al Azmeh, Fawaz</creator><creator>Montagna, Matteo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-4465-7146</orcidid><orcidid>https://orcid.org/0000-0002-5191-8298</orcidid></search><sort><creationdate>20190201</creationdate><title>Thermotolerant isolates of Beauveria bassiana as potential control agent of insect pest in subtropical climates</title><author>Alali, Sumer ; Mereghetti, Valeria ; Faoro, Franco ; Bocchi, Stefano ; Al Azmeh, Fawaz ; Montagna, Matteo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-cdd9f952c29a875cacdf9bd151395299d65b98f70a204e458768b862b68a760a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Agricultural pests</topic><topic>Agricultural production</topic><topic>Animals</topic><topic>Ascomycota</topic><topic>Beauveria - 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These limitations, further amplified in a global warming scenario, could nullify biological control strategies based on this fungus. The identification of thermotolerant B. bassiana isolates represents a possible strategy to overcome this problem. In this study, in order to maximize the probability in the isolation of thermotolerant B. bassiana, soil samples and infected insects were collected in warm areas of Syria. The obtained fungal isolates were tested for different biological parameters (i.e., growth rate, sporulation and spore germination) at growing temperatures ranging from 20°C to 35°C. Among these isolates (eight from insects and 11 from soil samples), the five with the highest growth rate, spore production and germination at 30°C were tested for their entomopathogenicity through in vivo assays on Ephestia kuehniella larvae. Insect mortality induced by the five isolates ranged from 31% to 100%. 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| ispartof | PloS one, 2019-02, Vol.14 (2), p.e0211457 |
| issn | 1932-6203 1932-6203 |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agricultural pests Agricultural production Animals Ascomycota Beauveria - classification Beauveria - genetics Beauveria - isolation & purification Beauveria - physiology Beauveria bassiana Biological control Biological pest control Biology and Life Sciences Biopesticides Climate Climate change Control Environmental factors Fungal Proteins - genetics Fungi Germination Global temperature changes Global warming Growth rate High temperature Humidity In vivo methods and tests Insect pests Insecta - growth & development Insecta - microbiology Insects Larva - microbiology Larvae Low humidity Mitochondrial DNA Morphology Mortality Peptide Elongation Factor 1 - genetics Pest control Pest Control, Biological - methods Pests Phylogenetics Phylogeny Plant diseases Research and Analysis Methods Soil Microbiology Soils Spore germination Sporulation Subtropical climates Syria Temperature effects Thermotolerance Tropical Climate |
| title | Thermotolerant isolates of Beauveria bassiana as potential control agent of insect pest in subtropical climates |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T18%3A56%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Thermotolerant%20isolates%20of%20Beauveria%20bassiana%20as%20potential%20control%20agent%20of%20insect%20pest%20in%20subtropical%20climates&rft.jtitle=PloS%20one&rft.au=Alali,%20Sumer&rft.date=2019-02-01&rft.volume=14&rft.issue=2&rft.spage=e0211457&rft.pages=e0211457-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0211457&rft_dat=%3Cgale_plos_%3EA572076763%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2174742195&rft_id=info:pmid/30707750&rft_galeid=A572076763&rft_doaj_id=oai_doaj_org_article_0d756762895a4cc3a69a4e68ecdbcf77&rfr_iscdi=true |