Induction of phytoalexins and proteins related to pathogenesis in plants treated with extracts of cutaneous secretions of southern Amazonian Bufonidae amphibians
Cutaneous secretions produced by amphibians of the family Bufonidae are rich sources of bioactive compounds that can be useful as new chemical templates for agrochemicals. In crop protection, the use of elicitors to induce responses offers the prospect of durable, broad-spectrum disease control usin...
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| Veröffentlicht in: | PloS one 2019-01, Vol.14 (1), p.e0211020 |
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| creator | Deice Raasch-Fernandes, Livia Bonaldo, Solange Maria de Jesus Rodrigues, Domingos Magela Vieira-Junior, Gerardo Regina Freitas Schwan-Estrada, Kátia Rocco da Silva, Camila Gabriela Araújo Verçosa, Ana Lopes de Oliveira, Daiane Wender Debiasi, Bryan |
| description | Cutaneous secretions produced by amphibians of the family Bufonidae are rich sources of bioactive compounds that can be useful as new chemical templates for agrochemicals. In crop protection, the use of elicitors to induce responses offers the prospect of durable, broad-spectrum disease control using the plant's own resistance. Therefore, we evaluated the potential of methanolic extracts of cutaneous secretions of two species of amphibians of the family Bufonidae found in the Amazon biome-Rhaebo guttatus (species 1) and Rhinella marina (species 2)-in the synthesis of phytoalexins in soybean cotyledons, bean hypocotyls, and sorghum mesocotyls. Additionally, changes in the enzyme activity of β-1,3-glucanase, peroxidase (POX), and polyphenol oxidase (PPO) and in the total protein content of soybean cotyledons were determined. In the soybean cultivar 'TMG 132 RR', our results indicated that the methanolic extract of R. guttatus cutaneous secretions suppressed glyceollin synthesis and β-1,3-glucanase activity and increased POX and PPO activities at higher concentrations and total protein content at a concentration of 0.2 mg/mL. On the other hand, the methanolic extract of R. marina cutaneous secretions induced glyceollin synthesis in the soybean cultivars 'TMG 132 RR' and 'Monsoy 8372 IPRO' at 0.1-0.2 mg/mL and 0.2 mg/mL, respectively. The methanolic extract of R. marina cutaneous secretions also increased the specific activity of POX and PPO in 'Monsoy 8372 IPRO' and 'TMG 132 RR', respectively, and decreased the activity of β-1,3-glucanases in 'Monsoy 8372 IPRO'. At 0.3 mg/mL, it stimulated phaseolin synthesis. The extracts did not express bioactivity in the synthesis of deoxyanthocyanidins in sorghum mesocotyls. The study in soybean suggests that the bioactivity in defense responses is influenced by cultivar genotypes. Therefore, these results provide evidence that extracts of cutaneous secretions of these amphibians species may contribute to the bioactivity of defense metabolites in plants. |
| doi_str_mv | 10.1371/journal.pone.0211020 |
| format | Article |
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In crop protection, the use of elicitors to induce responses offers the prospect of durable, broad-spectrum disease control using the plant's own resistance. Therefore, we evaluated the potential of methanolic extracts of cutaneous secretions of two species of amphibians of the family Bufonidae found in the Amazon biome-Rhaebo guttatus (species 1) and Rhinella marina (species 2)-in the synthesis of phytoalexins in soybean cotyledons, bean hypocotyls, and sorghum mesocotyls. Additionally, changes in the enzyme activity of β-1,3-glucanase, peroxidase (POX), and polyphenol oxidase (PPO) and in the total protein content of soybean cotyledons were determined. In the soybean cultivar 'TMG 132 RR', our results indicated that the methanolic extract of R. guttatus cutaneous secretions suppressed glyceollin synthesis and β-1,3-glucanase activity and increased POX and PPO activities at higher concentrations and total protein content at a concentration of 0.2 mg/mL. On the other hand, the methanolic extract of R. marina cutaneous secretions induced glyceollin synthesis in the soybean cultivars 'TMG 132 RR' and 'Monsoy 8372 IPRO' at 0.1-0.2 mg/mL and 0.2 mg/mL, respectively. The methanolic extract of R. marina cutaneous secretions also increased the specific activity of POX and PPO in 'Monsoy 8372 IPRO' and 'TMG 132 RR', respectively, and decreased the activity of β-1,3-glucanases in 'Monsoy 8372 IPRO'. At 0.3 mg/mL, it stimulated phaseolin synthesis. The extracts did not express bioactivity in the synthesis of deoxyanthocyanidins in sorghum mesocotyls. The study in soybean suggests that the bioactivity in defense responses is influenced by cultivar genotypes. Therefore, these results provide evidence that extracts of cutaneous secretions of these amphibians species may contribute to the bioactivity of defense metabolites in plants.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0211020</identifier><identifier>PMID: 30653617</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Agricultural chemicals ; Agrochemicals ; Amphibia ; Amphibian Proteins - chemistry ; Amphibian Proteins - pharmacology ; Amphibians ; Animals ; Bioactive compounds ; Biological activity ; Biology and Life Sciences ; Bodily secretions ; Bufonidae ; Bufonids ; Cane toad ; Chemical properties ; Cotyledons ; Crop diseases ; Crops ; Cultivars ; Disease control ; Environmental science ; Enzymatic activity ; Enzyme activity ; Enzymes ; Gene expression ; Genotypes ; Glycine max - metabolism ; Hypocotyls ; Kinases ; Medicine and Health Sciences ; Metabolites ; Natural products ; Organic chemistry ; Pathogenesis ; Peroxidase ; Pesticides ; Phaseolin ; Physiological aspects ; Physiology ; Phytoalexins ; Plant Diseases ; Plant extracts ; Plant metabolites ; Plant protection ; Plants (botany) ; Polyphenol oxidase ; Proteins ; Pterocarpans - biosynthesis ; Research and Analysis Methods ; Rhaebo guttatus ; Rhinella marina ; Secretions ; Sesquiterpenes - chemistry ; Sesquiterpenes - pharmacology ; Skin - chemistry ; Skin - metabolism ; Sorghum ; Soybeans ; Species ; Synthesis</subject><ispartof>PloS one, 2019-01, Vol.14 (1), p.e0211020</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Deice Raasch-Fernandes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Deice Raasch-Fernandes et al 2019 Deice Raasch-Fernandes et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-6391bba5cb1d5bb42d1978291e07514d8bc8d89a4459e048a726a86db907b2153</citedby><cites>FETCH-LOGICAL-c692t-6391bba5cb1d5bb42d1978291e07514d8bc8d89a4459e048a726a86db907b2153</cites><orcidid>0000-0002-2240-2700 ; 0000-0001-8926-2362</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336429/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336429/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30653617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deice Raasch-Fernandes, Livia</creatorcontrib><creatorcontrib>Bonaldo, Solange Maria</creatorcontrib><creatorcontrib>de Jesus Rodrigues, Domingos</creatorcontrib><creatorcontrib>Magela Vieira-Junior, Gerardo</creatorcontrib><creatorcontrib>Regina Freitas Schwan-Estrada, Kátia</creatorcontrib><creatorcontrib>Rocco da Silva, Camila</creatorcontrib><creatorcontrib>Gabriela Araújo Verçosa, Ana</creatorcontrib><creatorcontrib>Lopes de Oliveira, Daiane</creatorcontrib><creatorcontrib>Wender Debiasi, Bryan</creatorcontrib><title>Induction of phytoalexins and proteins related to pathogenesis in plants treated with extracts of cutaneous secretions of southern Amazonian Bufonidae amphibians</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Cutaneous secretions produced by amphibians of the family Bufonidae are rich sources of bioactive compounds that can be useful as new chemical templates for agrochemicals. In crop protection, the use of elicitors to induce responses offers the prospect of durable, broad-spectrum disease control using the plant's own resistance. Therefore, we evaluated the potential of methanolic extracts of cutaneous secretions of two species of amphibians of the family Bufonidae found in the Amazon biome-Rhaebo guttatus (species 1) and Rhinella marina (species 2)-in the synthesis of phytoalexins in soybean cotyledons, bean hypocotyls, and sorghum mesocotyls. Additionally, changes in the enzyme activity of β-1,3-glucanase, peroxidase (POX), and polyphenol oxidase (PPO) and in the total protein content of soybean cotyledons were determined. In the soybean cultivar 'TMG 132 RR', our results indicated that the methanolic extract of R. guttatus cutaneous secretions suppressed glyceollin synthesis and β-1,3-glucanase activity and increased POX and PPO activities at higher concentrations and total protein content at a concentration of 0.2 mg/mL. On the other hand, the methanolic extract of R. marina cutaneous secretions induced glyceollin synthesis in the soybean cultivars 'TMG 132 RR' and 'Monsoy 8372 IPRO' at 0.1-0.2 mg/mL and 0.2 mg/mL, respectively. The methanolic extract of R. marina cutaneous secretions also increased the specific activity of POX and PPO in 'Monsoy 8372 IPRO' and 'TMG 132 RR', respectively, and decreased the activity of β-1,3-glucanases in 'Monsoy 8372 IPRO'. At 0.3 mg/mL, it stimulated phaseolin synthesis. The extracts did not express bioactivity in the synthesis of deoxyanthocyanidins in sorghum mesocotyls. The study in soybean suggests that the bioactivity in defense responses is influenced by cultivar genotypes. Therefore, these results provide evidence that extracts of cutaneous secretions of these amphibians species may contribute to the bioactivity of defense metabolites in plants.</description><subject>Acids</subject><subject>Agricultural chemicals</subject><subject>Agrochemicals</subject><subject>Amphibia</subject><subject>Amphibian Proteins - chemistry</subject><subject>Amphibian Proteins - pharmacology</subject><subject>Amphibians</subject><subject>Animals</subject><subject>Bioactive compounds</subject><subject>Biological activity</subject><subject>Biology and Life Sciences</subject><subject>Bodily secretions</subject><subject>Bufonidae</subject><subject>Bufonids</subject><subject>Cane toad</subject><subject>Chemical properties</subject><subject>Cotyledons</subject><subject>Crop diseases</subject><subject>Crops</subject><subject>Cultivars</subject><subject>Disease control</subject><subject>Environmental science</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Genotypes</subject><subject>Glycine max - metabolism</subject><subject>Hypocotyls</subject><subject>Kinases</subject><subject>Medicine and Health Sciences</subject><subject>Metabolites</subject><subject>Natural products</subject><subject>Organic chemistry</subject><subject>Pathogenesis</subject><subject>Peroxidase</subject><subject>Pesticides</subject><subject>Phaseolin</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Phytoalexins</subject><subject>Plant Diseases</subject><subject>Plant extracts</subject><subject>Plant metabolites</subject><subject>Plant protection</subject><subject>Plants (botany)</subject><subject>Polyphenol oxidase</subject><subject>Proteins</subject><subject>Pterocarpans - biosynthesis</subject><subject>Research and Analysis Methods</subject><subject>Rhaebo guttatus</subject><subject>Rhinella marina</subject><subject>Secretions</subject><subject>Sesquiterpenes - chemistry</subject><subject>Sesquiterpenes - pharmacology</subject><subject>Skin - chemistry</subject><subject>Skin - metabolism</subject><subject>Sorghum</subject><subject>Soybeans</subject><subject>Species</subject><subject>Synthesis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk2uL1DAUhoso7rr6D0QDguCHGZOmTZsvwrh4GVhY8PY15HI6zdJJapLqrv_Gf2rmsssMKEg_NH37nDfhPTlF8ZTgOaENeX3lp-DkMB-9gzkuCcElvlecEk7LGSsxvX-wPikexXiFcU1bxh4WJxSzmjLSnBa_l85MOlnvkO_Q2N8kLwe4ti4i6Qwag0-w-QgwyAQGJY9GmXq_AgfRRmQdGgfpUkQpwJb4aVOP4DoFqbOaTfWUpAM_RRRBB9jstdWjn1IPwaHFWv7yzkqH3k5dXhgJSK7H3qqsxcfFg04OEZ7s32fF1_fvvpx_nF1cflieLy5mmvEyzRjlRClZa0VMrVRVGsKbtuQEcFOTyrRKt6blsqpqDrhqZVMy2TKjOG5USWp6Vjzf-Y6Dj2KfbhQlYS1pCcU4E8sdYby8EmOwaxluhJdWbAUfVkKGZPUAQqqaNByMqSpa0a7kjW4UqbmqcFvlg2avN_vdJrUGo8HlwIYj0-M_zvZi5X8IRimrSp4NXuwNgv8-QUz_OPKeWuWuCus6v-nL2kYtFjXjOSFG20zN_0Llx8Da6ny_Opv1o4JXRwWZSbnlKznFKJafP_0_e_ntmH15wPYgh9RHP0zbO3MMVjtQBx9jgO4uOYLFZjxu0xCb8RD78chlzw5Tvyu6nQf6B38eDnc</recordid><startdate>20190117</startdate><enddate>20190117</enddate><creator>Deice Raasch-Fernandes, Livia</creator><creator>Bonaldo, Solange Maria</creator><creator>de Jesus Rodrigues, Domingos</creator><creator>Magela Vieira-Junior, Gerardo</creator><creator>Regina Freitas Schwan-Estrada, Kátia</creator><creator>Rocco da Silva, Camila</creator><creator>Gabriela Araújo Verçosa, Ana</creator><creator>Lopes de Oliveira, Daiane</creator><creator>Wender Debiasi, Bryan</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-2240-2700</orcidid><orcidid>https://orcid.org/0000-0001-8926-2362</orcidid></search><sort><creationdate>20190117</creationdate><title>Induction of phytoalexins and proteins related to pathogenesis in plants treated with extracts of cutaneous secretions of southern Amazonian Bufonidae amphibians</title><author>Deice Raasch-Fernandes, Livia ; Bonaldo, Solange Maria ; de Jesus Rodrigues, Domingos ; Magela Vieira-Junior, Gerardo ; Regina Freitas Schwan-Estrada, Kátia ; Rocco da Silva, Camila ; Gabriela Araújo Verçosa, Ana ; Lopes de Oliveira, Daiane ; Wender Debiasi, Bryan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-6391bba5cb1d5bb42d1978291e07514d8bc8d89a4459e048a726a86db907b2153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acids</topic><topic>Agricultural chemicals</topic><topic>Agrochemicals</topic><topic>Amphibia</topic><topic>Amphibian Proteins - chemistry</topic><topic>Amphibian Proteins - pharmacology</topic><topic>Amphibians</topic><topic>Animals</topic><topic>Bioactive compounds</topic><topic>Biological activity</topic><topic>Biology and Life Sciences</topic><topic>Bodily secretions</topic><topic>Bufonidae</topic><topic>Bufonids</topic><topic>Cane toad</topic><topic>Chemical properties</topic><topic>Cotyledons</topic><topic>Crop diseases</topic><topic>Crops</topic><topic>Cultivars</topic><topic>Disease control</topic><topic>Environmental science</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Genotypes</topic><topic>Glycine max - metabolism</topic><topic>Hypocotyls</topic><topic>Kinases</topic><topic>Medicine and Health Sciences</topic><topic>Metabolites</topic><topic>Natural products</topic><topic>Organic chemistry</topic><topic>Pathogenesis</topic><topic>Peroxidase</topic><topic>Pesticides</topic><topic>Phaseolin</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Phytoalexins</topic><topic>Plant Diseases</topic><topic>Plant extracts</topic><topic>Plant metabolites</topic><topic>Plant protection</topic><topic>Plants (botany)</topic><topic>Polyphenol oxidase</topic><topic>Proteins</topic><topic>Pterocarpans - biosynthesis</topic><topic>Research and Analysis Methods</topic><topic>Rhaebo guttatus</topic><topic>Rhinella marina</topic><topic>Secretions</topic><topic>Sesquiterpenes - chemistry</topic><topic>Sesquiterpenes - pharmacology</topic><topic>Skin - chemistry</topic><topic>Skin - metabolism</topic><topic>Sorghum</topic><topic>Soybeans</topic><topic>Species</topic><topic>Synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deice Raasch-Fernandes, Livia</creatorcontrib><creatorcontrib>Bonaldo, Solange Maria</creatorcontrib><creatorcontrib>de Jesus Rodrigues, Domingos</creatorcontrib><creatorcontrib>Magela Vieira-Junior, Gerardo</creatorcontrib><creatorcontrib>Regina Freitas Schwan-Estrada, Kátia</creatorcontrib><creatorcontrib>Rocco da Silva, Camila</creatorcontrib><creatorcontrib>Gabriela Araújo Verçosa, Ana</creatorcontrib><creatorcontrib>Lopes de Oliveira, Daiane</creatorcontrib><creatorcontrib>Wender Debiasi, Bryan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deice Raasch-Fernandes, Livia</au><au>Bonaldo, Solange Maria</au><au>de Jesus Rodrigues, Domingos</au><au>Magela Vieira-Junior, Gerardo</au><au>Regina Freitas Schwan-Estrada, Kátia</au><au>Rocco da Silva, Camila</au><au>Gabriela Araújo Verçosa, Ana</au><au>Lopes de Oliveira, Daiane</au><au>Wender Debiasi, Bryan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of phytoalexins and proteins related to pathogenesis in plants treated with extracts of cutaneous secretions of southern Amazonian Bufonidae amphibians</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-01-17</date><risdate>2019</risdate><volume>14</volume><issue>1</issue><spage>e0211020</spage><pages>e0211020-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cutaneous secretions produced by amphibians of the family Bufonidae are rich sources of bioactive compounds that can be useful as new chemical templates for agrochemicals. In crop protection, the use of elicitors to induce responses offers the prospect of durable, broad-spectrum disease control using the plant's own resistance. Therefore, we evaluated the potential of methanolic extracts of cutaneous secretions of two species of amphibians of the family Bufonidae found in the Amazon biome-Rhaebo guttatus (species 1) and Rhinella marina (species 2)-in the synthesis of phytoalexins in soybean cotyledons, bean hypocotyls, and sorghum mesocotyls. Additionally, changes in the enzyme activity of β-1,3-glucanase, peroxidase (POX), and polyphenol oxidase (PPO) and in the total protein content of soybean cotyledons were determined. In the soybean cultivar 'TMG 132 RR', our results indicated that the methanolic extract of R. guttatus cutaneous secretions suppressed glyceollin synthesis and β-1,3-glucanase activity and increased POX and PPO activities at higher concentrations and total protein content at a concentration of 0.2 mg/mL. On the other hand, the methanolic extract of R. marina cutaneous secretions induced glyceollin synthesis in the soybean cultivars 'TMG 132 RR' and 'Monsoy 8372 IPRO' at 0.1-0.2 mg/mL and 0.2 mg/mL, respectively. The methanolic extract of R. marina cutaneous secretions also increased the specific activity of POX and PPO in 'Monsoy 8372 IPRO' and 'TMG 132 RR', respectively, and decreased the activity of β-1,3-glucanases in 'Monsoy 8372 IPRO'. At 0.3 mg/mL, it stimulated phaseolin synthesis. The extracts did not express bioactivity in the synthesis of deoxyanthocyanidins in sorghum mesocotyls. The study in soybean suggests that the bioactivity in defense responses is influenced by cultivar genotypes. Therefore, these results provide evidence that extracts of cutaneous secretions of these amphibians species may contribute to the bioactivity of defense metabolites in plants.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30653617</pmid><doi>10.1371/journal.pone.0211020</doi><tpages>e0211020</tpages><orcidid>https://orcid.org/0000-0002-2240-2700</orcidid><orcidid>https://orcid.org/0000-0001-8926-2362</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-01, Vol.14 (1), p.e0211020 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2168181300 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Agricultural chemicals Agrochemicals Amphibia Amphibian Proteins - chemistry Amphibian Proteins - pharmacology Amphibians Animals Bioactive compounds Biological activity Biology and Life Sciences Bodily secretions Bufonidae Bufonids Cane toad Chemical properties Cotyledons Crop diseases Crops Cultivars Disease control Environmental science Enzymatic activity Enzyme activity Enzymes Gene expression Genotypes Glycine max - metabolism Hypocotyls Kinases Medicine and Health Sciences Metabolites Natural products Organic chemistry Pathogenesis Peroxidase Pesticides Phaseolin Physiological aspects Physiology Phytoalexins Plant Diseases Plant extracts Plant metabolites Plant protection Plants (botany) Polyphenol oxidase Proteins Pterocarpans - biosynthesis Research and Analysis Methods Rhaebo guttatus Rhinella marina Secretions Sesquiterpenes - chemistry Sesquiterpenes - pharmacology Skin - chemistry Skin - metabolism Sorghum Soybeans Species Synthesis |
| title | Induction of phytoalexins and proteins related to pathogenesis in plants treated with extracts of cutaneous secretions of southern Amazonian Bufonidae amphibians |
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