Third harmonic generation imaging and analysis of the effect of low gravity on the lacuno-canalicular network of mouse bone
The lacuno-canalicular network (LCN) hosting the osteocytes in bone tissue represents a biological signature of the mechanotransduction activity in response to external biomechanical loading. Using third-harmonic generation (THG) microscopy with sub-micrometer resolution, we investigate the impact o...
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| Veröffentlicht in: | PloS one 2019-01, Vol.14 (1), p.e0209079-e0209079 |
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| creator | Genthial, Rachel Gerbaix, Maude Farlay, Delphine Vico, Laurence Beaurepaire, Emmanuel Débarre, Delphine Gourrier, Aurélien |
| description | The lacuno-canalicular network (LCN) hosting the osteocytes in bone tissue represents a biological signature of the mechanotransduction activity in response to external biomechanical loading. Using third-harmonic generation (THG) microscopy with sub-micrometer resolution, we investigate the impact of microgravity on the 3D LCN structure in mice following space flight. A specific analytical procedure to extract the LCN characteristics from THG images is described for ex vivo studies of bone sections. The analysis conducted in different anatomical quadrants of femoral cortical bone didn't reveal any statistical differences between the control, habitat control and flight groups, suggesting that the LCN connectivity is not affected by one month space flight. However, significant variations are systematically observed within each sample. We show that our current lack of understanding of the extent of the LCN heterogeneity at the organ level hinders the interpretation of such investigations based on a limited number of samples and we discuss the implications for future biomedical studies. |
| doi_str_mv | 10.1371/journal.pone.0209079 |
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Using third-harmonic generation (THG) microscopy with sub-micrometer resolution, we investigate the impact of microgravity on the 3D LCN structure in mice following space flight. A specific analytical procedure to extract the LCN characteristics from THG images is described for ex vivo studies of bone sections. The analysis conducted in different anatomical quadrants of femoral cortical bone didn't reveal any statistical differences between the control, habitat control and flight groups, suggesting that the LCN connectivity is not affected by one month space flight. However, significant variations are systematically observed within each sample. We show that our current lack of understanding of the extent of the LCN heterogeneity at the organ level hinders the interpretation of such investigations based on a limited number of samples and we discuss the implications for future biomedical studies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0209079</identifier><identifier>PMID: 30601851</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Biocompatibility ; Biology ; Biology and Life Sciences ; Biomechanics ; Biomedical materials ; Bones ; Cell adhesion & migration ; Cell cycle ; Cortical bone ; Ecosystem ; Engineering and Technology ; Extracellular matrix ; Femur ; Harmonic generations ; Heterogeneity ; Hypogravity ; Laboratory rats ; Loading ; Male ; Mechanical loading ; Mechanotransduction ; Medicine and Health Sciences ; Methods ; Mice ; Mice, Inbred C57BL ; Microgravity ; Microscopy ; Microscopy, Confocal - methods ; Optics ; Osteocytes ; Physical Sciences ; Quadrants ; Research and Analysis Methods ; Space flight ; Statistical analysis ; Statistical methods ; Weightlessness</subject><ispartof>PloS one, 2019-01, Vol.14 (1), p.e0209079-e0209079</ispartof><rights>COPYRIGHT 2019 Public Library of Science</rights><rights>2019 Genthial et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Using third-harmonic generation (THG) microscopy with sub-micrometer resolution, we investigate the impact of microgravity on the 3D LCN structure in mice following space flight. A specific analytical procedure to extract the LCN characteristics from THG images is described for ex vivo studies of bone sections. The analysis conducted in different anatomical quadrants of femoral cortical bone didn't reveal any statistical differences between the control, habitat control and flight groups, suggesting that the LCN connectivity is not affected by one month space flight. However, significant variations are systematically observed within each sample. We show that our current lack of understanding of the extent of the LCN heterogeneity at the organ level hinders the interpretation of such investigations based on a limited number of samples and we discuss the implications for future biomedical studies.</description><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Biomechanics</subject><subject>Biomedical materials</subject><subject>Bones</subject><subject>Cell adhesion & migration</subject><subject>Cell cycle</subject><subject>Cortical bone</subject><subject>Ecosystem</subject><subject>Engineering and Technology</subject><subject>Extracellular matrix</subject><subject>Femur</subject><subject>Harmonic generations</subject><subject>Heterogeneity</subject><subject>Hypogravity</subject><subject>Laboratory rats</subject><subject>Loading</subject><subject>Male</subject><subject>Mechanical loading</subject><subject>Mechanotransduction</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microgravity</subject><subject>Microscopy</subject><subject>Microscopy, Confocal - 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Using third-harmonic generation (THG) microscopy with sub-micrometer resolution, we investigate the impact of microgravity on the 3D LCN structure in mice following space flight. A specific analytical procedure to extract the LCN characteristics from THG images is described for ex vivo studies of bone sections. The analysis conducted in different anatomical quadrants of femoral cortical bone didn't reveal any statistical differences between the control, habitat control and flight groups, suggesting that the LCN connectivity is not affected by one month space flight. However, significant variations are systematically observed within each sample. We show that our current lack of understanding of the extent of the LCN heterogeneity at the organ level hinders the interpretation of such investigations based on a limited number of samples and we discuss the implications for future biomedical studies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30601851</pmid><doi>10.1371/journal.pone.0209079</doi><tpages>e0209079</tpages><orcidid>https://orcid.org/0000-0001-9526-5746</orcidid><orcidid>https://orcid.org/0000-0002-2082-8214</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biocompatibility Biology Biology and Life Sciences Biomechanics Biomedical materials Bones Cell adhesion & migration Cell cycle Cortical bone Ecosystem Engineering and Technology Extracellular matrix Femur Harmonic generations Heterogeneity Hypogravity Laboratory rats Loading Male Mechanical loading Mechanotransduction Medicine and Health Sciences Methods Mice Mice, Inbred C57BL Microgravity Microscopy Microscopy, Confocal - methods Optics Osteocytes Physical Sciences Quadrants Research and Analysis Methods Space flight Statistical analysis Statistical methods Weightlessness |
| title | Third harmonic generation imaging and analysis of the effect of low gravity on the lacuno-canalicular network of mouse bone |
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