Impact of mydriasis in fluorescence lifetime imaging ophthalmoscopy
Fluorescence lifetime imaging ophthalmoscopy (FLIO) is a novel technique that measures in vivo autofluorescence intensity decay over time of endogenous fluorophores in the retina. The Heidelberg Engineering FLIO system was used to obtain two 30 degree scans centered on the fovea of both eyes. The FL...
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| description | Fluorescence lifetime imaging ophthalmoscopy (FLIO) is a novel technique that measures in vivo autofluorescence intensity decay over time of endogenous fluorophores in the retina. The Heidelberg Engineering FLIO system was used to obtain two 30 degree scans centered on the fovea of both eyes. The FLIO system uses a 473nm blue scanning laser light source and the emitted fluorescence is detected in two wavelengths channels, short and long spectral channels (SSC, LSC). Since the mydriatic status influence the FLIO result, the impact of mydriasis on FLIO need to be clarified. In this prospective, observational study, the impact of mydriasis on measurements from fluorescence lifetime imaging ophthalmoscope (FLIO) images in normal subjects were evaluated. 12 healthy participants (24 eyes) were volunteered and all subjects were scanned twice and the mean fluorescence lifetime (τm) values were computed with dilation and without dilation on different days. Intraclass correlation coefficients (ICC) and coefficients of variation (CV) were calculated from the measured τm in dilated, nondilated and between the dilated and non-dilated setting. Test duration was also compared and correlated with lifetimes in both settings. Repeatability was excellent for both the dilation and non-dilation settings (ICC; 0.967-0.996; 0.926-0.986, respectively). The agreement between the dilation and non-dilation settings, however, were lower (ICC; 0.688-0.970). The τm in the non-dilation setting was significantly longer than in the dilation setting for the SSC (P |
| doi_str_mv | 10.1371/journal.pone.0209194 |
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The Heidelberg Engineering FLIO system was used to obtain two 30 degree scans centered on the fovea of both eyes. The FLIO system uses a 473nm blue scanning laser light source and the emitted fluorescence is detected in two wavelengths channels, short and long spectral channels (SSC, LSC). Since the mydriatic status influence the FLIO result, the impact of mydriasis on FLIO need to be clarified. In this prospective, observational study, the impact of mydriasis on measurements from fluorescence lifetime imaging ophthalmoscope (FLIO) images in normal subjects were evaluated. 12 healthy participants (24 eyes) were volunteered and all subjects were scanned twice and the mean fluorescence lifetime (τm) values were computed with dilation and without dilation on different days. Intraclass correlation coefficients (ICC) and coefficients of variation (CV) were calculated from the measured τm in dilated, nondilated and between the dilated and non-dilated setting. Test duration was also compared and correlated with lifetimes in both settings. Repeatability was excellent for both the dilation and non-dilation settings (ICC; 0.967-0.996; 0.926-0.986, respectively). The agreement between the dilation and non-dilation settings, however, were lower (ICC; 0.688-0.970). The τm in the non-dilation setting was significantly longer than in the dilation setting for the SSC (P<0.05). The FLIO test duration in the non-dilation setting was significantly longer than with dilation for the SSC (P <0.05). Although good repeatability in τm measurements between imaging sessions were observed both with and without dilation, the agreement was not as good when comparing dilated with non-dilated measurements. Since FLIO without mydriasis results in longer τm in the SSC and takes a longer time for image acquisition, maximal dilation is recommended for FLIO testing.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0209194</identifier><identifier>PMID: 30592718</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Alzheimer's disease ; Alzheimers disease ; Biology and Life Sciences ; Channels ; Chemical compounds ; Coefficient of variation ; Correlation coefficient ; Correlation coefficients ; Diabetes ; Diabetic retinopathy ; Diagnosis ; Dilation ; Eye ; Eye (anatomy) ; Female ; Fluorescence ; Fluorophores ; Fovea ; Humans ; Image acquisition ; In vivo methods and tests ; Lifetime ; Light sources ; Macular degeneration ; Male ; Mathematical analysis ; Medical imaging ; Medicine ; Medicine and Health Sciences ; Mydriasis ; Ophthalmoscopes ; Ophthalmoscopy - methods ; Optical Imaging - methods ; Physical Sciences ; Prospective Studies ; Pupil ; Reproducibility ; Reproducibility of Results ; Research and Analysis Methods ; Retina ; Retina - diagnostic imaging ; Retinal diseases ; Time Factors ; Wavelengths</subject><ispartof>PloS one, 2018-12, Vol.13 (12), p.e0209194-e0209194</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Sadda et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Sadda et al 2018 Sadda et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-132e33c70e910b620daacd5b34a3fae85e9d33d86d443adddcbdd2610845a0353</citedby><cites>FETCH-LOGICAL-c758t-132e33c70e910b620daacd5b34a3fae85e9d33d86d443adddcbdd2610845a0353</cites><orcidid>0000-0003-1543-981X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310355/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310355/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30592718$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Awadein, Ahmed</contributor><creatorcontrib>Sadda, SriniVas R</creatorcontrib><creatorcontrib>Borrelli, Enrico</creatorcontrib><creatorcontrib>Fan, Wenying</creatorcontrib><creatorcontrib>Ebraheem, Adel</creatorcontrib><creatorcontrib>Marion, Kenneth M</creatorcontrib><creatorcontrib>Kwon, Soonil</creatorcontrib><title>Impact of mydriasis in fluorescence lifetime imaging ophthalmoscopy</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Fluorescence lifetime imaging ophthalmoscopy (FLIO) is a novel technique that measures in vivo autofluorescence intensity decay over time of endogenous fluorophores in the retina. 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Test duration was also compared and correlated with lifetimes in both settings. Repeatability was excellent for both the dilation and non-dilation settings (ICC; 0.967-0.996; 0.926-0.986, respectively). The agreement between the dilation and non-dilation settings, however, were lower (ICC; 0.688-0.970). The τm in the non-dilation setting was significantly longer than in the dilation setting for the SSC (P<0.05). The FLIO test duration in the non-dilation setting was significantly longer than with dilation for the SSC (P <0.05). Although good repeatability in τm measurements between imaging sessions were observed both with and without dilation, the agreement was not as good when comparing dilated with non-dilated measurements. 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The Heidelberg Engineering FLIO system was used to obtain two 30 degree scans centered on the fovea of both eyes. The FLIO system uses a 473nm blue scanning laser light source and the emitted fluorescence is detected in two wavelengths channels, short and long spectral channels (SSC, LSC). Since the mydriatic status influence the FLIO result, the impact of mydriasis on FLIO need to be clarified. In this prospective, observational study, the impact of mydriasis on measurements from fluorescence lifetime imaging ophthalmoscope (FLIO) images in normal subjects were evaluated. 12 healthy participants (24 eyes) were volunteered and all subjects were scanned twice and the mean fluorescence lifetime (τm) values were computed with dilation and without dilation on different days. Intraclass correlation coefficients (ICC) and coefficients of variation (CV) were calculated from the measured τm in dilated, nondilated and between the dilated and non-dilated setting. Test duration was also compared and correlated with lifetimes in both settings. Repeatability was excellent for both the dilation and non-dilation settings (ICC; 0.967-0.996; 0.926-0.986, respectively). The agreement between the dilation and non-dilation settings, however, were lower (ICC; 0.688-0.970). The τm in the non-dilation setting was significantly longer than in the dilation setting for the SSC (P<0.05). The FLIO test duration in the non-dilation setting was significantly longer than with dilation for the SSC (P <0.05). Although good repeatability in τm measurements between imaging sessions were observed both with and without dilation, the agreement was not as good when comparing dilated with non-dilated measurements. Since FLIO without mydriasis results in longer τm in the SSC and takes a longer time for image acquisition, maximal dilation is recommended for FLIO testing.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30592718</pmid><doi>10.1371/journal.pone.0209194</doi><tpages>e0209194</tpages><orcidid>https://orcid.org/0000-0003-1543-981X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Alzheimer's disease Alzheimers disease Biology and Life Sciences Channels Chemical compounds Coefficient of variation Correlation coefficient Correlation coefficients Diabetes Diabetic retinopathy Diagnosis Dilation Eye Eye (anatomy) Female Fluorescence Fluorophores Fovea Humans Image acquisition In vivo methods and tests Lifetime Light sources Macular degeneration Male Mathematical analysis Medical imaging Medicine Medicine and Health Sciences Mydriasis Ophthalmoscopes Ophthalmoscopy - methods Optical Imaging - methods Physical Sciences Prospective Studies Pupil Reproducibility Reproducibility of Results Research and Analysis Methods Retina Retina - diagnostic imaging Retinal diseases Time Factors Wavelengths |
| title | Impact of mydriasis in fluorescence lifetime imaging ophthalmoscopy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T18%3A03%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Impact%20of%20mydriasis%20in%20fluorescence%20lifetime%20imaging%20ophthalmoscopy&rft.jtitle=PloS%20one&rft.au=Sadda,%20SriniVas%20R&rft.date=2018-12-28&rft.volume=13&rft.issue=12&rft.spage=e0209194&rft.epage=e0209194&rft.pages=e0209194-e0209194&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0209194&rft_dat=%3Cgale_plos_%3EA567605972%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2161292492&rft_id=info:pmid/30592718&rft_galeid=A567605972&rft_doaj_id=oai_doaj_org_article_6e68215daf7a4490afff927f5e5b8349&rfr_iscdi=true |