High-resolution antibody array analysis of proteins from primary human keratinocytes and leukocytes

Antibody array analysis of labeled proteomes has high throughput and is simple to perform, but validation remains challenging. Here, we used differential detergent fractionation and size exclusion chromatography in sequence for high-resolution separation of biotinylated proteins from human primary k...

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Veröffentlicht in:	PloS one 2018-12, Vol.13 (12), p.e0209271-e0209271
Hauptverfasser:	de la Rosa Carrillo, Daniel, Sikorski, Krzysztof, Khnykin, Denis, Wu, Weiwei, Lund-Johansen, Fridtjof
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Antibodies Arrays Biology Biology and Life Sciences Biotinylation Cellular proteins Chromatography Chromatography, Gel Cytosol Dermatology Flow Cytometry Fractionation Gene Expression High resolution High resolution spectroscopy Humans Immunoglobulins Immunology Immunotherapy Keratinocytes Keratinocytes - cytology Keratinocytes - metabolism Leukocytes Leukocytes - cytology Leukocytes - metabolism Mass spectrometry Medicine and Health Sciences Membranes Methods Microspheres Middle Aged Organelles Physical Sciences Primary Cell Culture Protein Array Analysis - methods Protein Binding Proteins Proteins - immunology Proteins - metabolism Proteomics Reactivity Research and Analysis Methods RNA polymerase Scientific imaging Signal transduction Size exclusion chromatography Skin - cytology Skin - metabolism White blood cells
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