Cloning of the pks3 gene of Aurantiochytrium limacinum and functional study of the 3-ketoacyl-ACP reductase and dehydratase enzyme domains

Aurantiochytrium limacinum has received attention because of its abundance of polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA). DHA is synthesized through the polyketide synthase (PKS) pathway in A. limacinum. The related enzymes of the PKS pathway are mainly expressed by...

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Veröffentlicht in:	PloS one 2018-12, Vol.13 (12), p.e0208853-e0208853
Hauptverfasser:	Liu, Zhu, Zang, Xiaonan, Cao, Xuexue, Wang, Zhendong, Liu, Chang, Sun, Deguang, Guo, Yalin, Zhang, Feng, Yang, Qin, Hou, Pan, Pang, Chunhong
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acid sequence Amino acids Biology and Life Sciences Biosynthesis Biotechnology Chytrids Cloning Cloning, Molecular Dehydration Docosahexaenoic acid Docosahexaenoic Acids - metabolism Domains E coli Education Engineering and Technology Enzymes Fatty acids Fish oils Gene clusters Gene expression Gene mapping Genes Genetic aspects Genetics Genomes Genomics Glucose Homologous recombination Homology Hydro-Lyases - genetics Industrial applications Industrial equipment Laboratories Lipids Medicine and Health Sciences Metabolic syndrome Nicotinamide adenine dinucleotide phosphate Omega 3 fatty acids Oxidoreductases - genetics Plasmids Polyketide synthase Polymerase chain reaction Polyunsaturated fatty acids Reductase Research and Analysis Methods Salinity Seawater Stramenopiles - genetics Technology Tuberculosis Unsaturated fatty acids
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