Anthocyanins from Cornus kousa ethanolic extract attenuate obesity in association with anti-angiogenic activities in 3T3-L1 cells by down-regulating adipogeneses and lipogenesis
Cornus kousa the Korean dogwood has been traditionally used in East Asia as therapeutic traditional medicine however biological activities of Cornus kousa have not been investigated previously. The aim of the present study was to evaluate anti-obesity activities coupled with anti-angiogenic activiti...
Gespeichert in:
| Veröffentlicht in: | PloS one 2018-12, Vol.13 (12), p.e0208556-e0208556 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0208556 |
|---|---|
| container_issue | 12 |
| container_start_page | e0208556 |
| container_title | PloS one |
| container_volume | 13 |
| creator | Khan, Muhammad Imran Shin, Jin Hyuk Shin, Tai Sun Kim, Min Yong Cho, Nam Jun Kim, Jong Deog |
| description | Cornus kousa the Korean dogwood has been traditionally used in East Asia as therapeutic traditional medicine however biological activities of Cornus kousa have not been investigated previously. The aim of the present study was to evaluate anti-obesity activities coupled with anti-angiogenic activities of anthocyanins rich fraction of ethanolic leaf extract of Cornus kousa (ELECk) in HUVECs and 3T3- L1 cells. Dried plants leaves were extracted with 70% ethanol and anthocyanin fraction (AnT Fr) was obtained by eluting the ethanolic extract through non-polar macroporous resin and further purification by HPLC. Antiangiogenic activities were determined by antiproliferative effect of AnT Fr on HUVECs. In the presence of various concentrations of AnT Fr, 3T3-L1 preadipocytes were induced to differentiate. Lipid accumulation in differentiated adipocytes were quantified by Oil-Red O staining. AnT Fr significantly suppressed angiogenesis by inhibiting proliferation and tube formation of HUVECs via downregulating VEGRF 2, PI3K, β-catenin, NF-kB, and Akt1 in a dose dependent manner. AnT Fr inhibited lipid accumulation by down-regulating adipogenesis and lipogenesis promoting signaling proteins, PPARγ, CCAAT, C/EBPα, aP2, FAS, and LPL, however enhanced AMPK activation to p-AMPK in 3T3 cells quantified and expressed by western blotting. AnT Fr inhibit lipid accumulation by regulating adipogenesis and lipogenesis related genes and signaling proteins. The anti-obesity activities exerted by Cornus kousa are associated with antiangiogenic activities of anthocyanins rich fraction of Cornus kousa. Hence the presence of bioactive anthocyanins, Cornus kosa, is a good candidate for nutraceutical and pharmaceutical formulation for treating or controlling obesity. |
| doi_str_mv | 10.1371/journal.pone.0208556 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2151196754</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A564549566</galeid><doaj_id>oai_doaj_org_article_ed723772450d4cbd978865482415c6bb</doaj_id><sourcerecordid>A564549566</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-686af3cd3b60e13465070558839e4a056b1afa033ede78b5fadb9a6e9aeba7fd3</originalsourceid><addsrcrecordid>eNqNk91u1DAQhSMEoqXwBggsISG4yGLHsZPcIK0qfipVqgSFW2tiT7IuWXuJnZZ9LN4Qh26rLuoFykVi5zsn8ZmZLHvO6ILxir278NPoYFhsvMMFLWgthHyQHbKGF7ksKH945_kgexLCBaWC11I-zg44FQWTVBxmv5currzegrMukG70a3LsRzcF8sNPAQjGFTg_WE3wVxxBRwIxopsgIvEtBhu3xDoCIXhtIVrvyJWNKwIu2hxcb32PLqmT0l7aaDHMOD_n-SkjGochkHZLjL9y-Yj9NCQL1xMwdjMLMSQenCHDzdqGp9mjDoaAz3b3o-zbxw_nx5_z07NPJ8fL01zLpoi5rCV0XBveSoqMl1LQigpR17zBEqiQLYMOKOdosKpb0YFpG5DYALZQdYYfZS-vfTeDD2qXdlAFE4w1shJlIk6uCePhQm1Gu4ZxqzxY9XfDj72CMVo9oEJTFbyqilJQU-rWNFVdS1HWRcmElm2bvN7vvja1azQaXUp72DPdf-PsSvX-Usmi5rJkyeDNzmD0PycMUa1tmAMGh6mS83-nbmCiqhL66h_0_tPtqB7SAazr_Fz_2VQthSxF2QgpE7W4h0qXwbXVqTU7m_b3BG_3BImJqbV6mEJQJ1-__D979n2ffX2HXSEMcRX8MM0tGfbB8hrUow9hxO42ZEbVPFk3aah5stRuspLsxd0C3YpuRon_ARwcIlU</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2151196754</pqid></control><display><type>article</type><title>Anthocyanins from Cornus kousa ethanolic extract attenuate obesity in association with anti-angiogenic activities in 3T3-L1 cells by down-regulating adipogeneses and lipogenesis</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Khan, Muhammad Imran ; Shin, Jin Hyuk ; Shin, Tai Sun ; Kim, Min Yong ; Cho, Nam Jun ; Kim, Jong Deog</creator><contributor>López Lluch, Guillermo</contributor><creatorcontrib>Khan, Muhammad Imran ; Shin, Jin Hyuk ; Shin, Tai Sun ; Kim, Min Yong ; Cho, Nam Jun ; Kim, Jong Deog ; López Lluch, Guillermo</creatorcontrib><description>Cornus kousa the Korean dogwood has been traditionally used in East Asia as therapeutic traditional medicine however biological activities of Cornus kousa have not been investigated previously. The aim of the present study was to evaluate anti-obesity activities coupled with anti-angiogenic activities of anthocyanins rich fraction of ethanolic leaf extract of Cornus kousa (ELECk) in HUVECs and 3T3- L1 cells. Dried plants leaves were extracted with 70% ethanol and anthocyanin fraction (AnT Fr) was obtained by eluting the ethanolic extract through non-polar macroporous resin and further purification by HPLC. Antiangiogenic activities were determined by antiproliferative effect of AnT Fr on HUVECs. In the presence of various concentrations of AnT Fr, 3T3-L1 preadipocytes were induced to differentiate. Lipid accumulation in differentiated adipocytes were quantified by Oil-Red O staining. AnT Fr significantly suppressed angiogenesis by inhibiting proliferation and tube formation of HUVECs via downregulating VEGRF 2, PI3K, β-catenin, NF-kB, and Akt1 in a dose dependent manner. AnT Fr inhibited lipid accumulation by down-regulating adipogenesis and lipogenesis promoting signaling proteins, PPARγ, CCAAT, C/EBPα, aP2, FAS, and LPL, however enhanced AMPK activation to p-AMPK in 3T3 cells quantified and expressed by western blotting. AnT Fr inhibit lipid accumulation by regulating adipogenesis and lipogenesis related genes and signaling proteins. The anti-obesity activities exerted by Cornus kousa are associated with antiangiogenic activities of anthocyanins rich fraction of Cornus kousa. Hence the presence of bioactive anthocyanins, Cornus kosa, is a good candidate for nutraceutical and pharmaceutical formulation for treating or controlling obesity.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0208556</identifier><identifier>PMID: 30521605</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>1-Phosphatidylinositol 3-kinase ; Accumulation ; Adipocytes ; Adipogenesis ; Adipogenesis - drug effects ; AKT1 protein ; Angiogenesis ; Animals ; Anthocyanins ; Anthocyanins - isolation & purification ; Anthocyanins - pharmacology ; Antiangiogenics ; Antineoplastic agents ; Biology and Life Sciences ; Biotechnology ; Cancer ; Cornus - chemistry ; Cornus kousa ; Diabetes ; Down-Regulation - drug effects ; Ethanol ; Fatty acids ; Food ; Functional foods ; Functional foods & nutraceuticals ; Gene expression ; Genes ; Health aspects ; Herbal medicine ; High performance liquid chromatography ; Human Umbilical Vein Endothelial Cells ; Humans ; Kinases ; Leaves ; Lipids ; Lipogenesis ; Lipogenesis - drug effects ; Liquid chromatography ; Medicine and Health Sciences ; Metabolic disorders ; Metastasis ; Mice ; Natural products ; NIH 3T3 Cells ; Obesity ; Obesity - drug therapy ; Physiology ; Phytochemicals ; Plant cells ; Plant extracts ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Preadipocytes ; Proteins ; Research and analysis methods ; Signaling ; Weight control ; Western blotting</subject><ispartof>PloS one, 2018-12, Vol.13 (12), p.e0208556-e0208556</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Khan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Khan et al 2018 Khan et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-686af3cd3b60e13465070558839e4a056b1afa033ede78b5fadb9a6e9aeba7fd3</citedby><cites>FETCH-LOGICAL-c692t-686af3cd3b60e13465070558839e4a056b1afa033ede78b5fadb9a6e9aeba7fd3</cites><orcidid>0000-0003-1096-1529</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283641/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283641/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30521605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>López Lluch, Guillermo</contributor><creatorcontrib>Khan, Muhammad Imran</creatorcontrib><creatorcontrib>Shin, Jin Hyuk</creatorcontrib><creatorcontrib>Shin, Tai Sun</creatorcontrib><creatorcontrib>Kim, Min Yong</creatorcontrib><creatorcontrib>Cho, Nam Jun</creatorcontrib><creatorcontrib>Kim, Jong Deog</creatorcontrib><title>Anthocyanins from Cornus kousa ethanolic extract attenuate obesity in association with anti-angiogenic activities in 3T3-L1 cells by down-regulating adipogeneses and lipogenesis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Cornus kousa the Korean dogwood has been traditionally used in East Asia as therapeutic traditional medicine however biological activities of Cornus kousa have not been investigated previously. The aim of the present study was to evaluate anti-obesity activities coupled with anti-angiogenic activities of anthocyanins rich fraction of ethanolic leaf extract of Cornus kousa (ELECk) in HUVECs and 3T3- L1 cells. Dried plants leaves were extracted with 70% ethanol and anthocyanin fraction (AnT Fr) was obtained by eluting the ethanolic extract through non-polar macroporous resin and further purification by HPLC. Antiangiogenic activities were determined by antiproliferative effect of AnT Fr on HUVECs. In the presence of various concentrations of AnT Fr, 3T3-L1 preadipocytes were induced to differentiate. Lipid accumulation in differentiated adipocytes were quantified by Oil-Red O staining. AnT Fr significantly suppressed angiogenesis by inhibiting proliferation and tube formation of HUVECs via downregulating VEGRF 2, PI3K, β-catenin, NF-kB, and Akt1 in a dose dependent manner. AnT Fr inhibited lipid accumulation by down-regulating adipogenesis and lipogenesis promoting signaling proteins, PPARγ, CCAAT, C/EBPα, aP2, FAS, and LPL, however enhanced AMPK activation to p-AMPK in 3T3 cells quantified and expressed by western blotting. AnT Fr inhibit lipid accumulation by regulating adipogenesis and lipogenesis related genes and signaling proteins. The anti-obesity activities exerted by Cornus kousa are associated with antiangiogenic activities of anthocyanins rich fraction of Cornus kousa. Hence the presence of bioactive anthocyanins, Cornus kosa, is a good candidate for nutraceutical and pharmaceutical formulation for treating or controlling obesity.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>Accumulation</subject><subject>Adipocytes</subject><subject>Adipogenesis</subject><subject>Adipogenesis - drug effects</subject><subject>AKT1 protein</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Anthocyanins</subject><subject>Anthocyanins - isolation & purification</subject><subject>Anthocyanins - pharmacology</subject><subject>Antiangiogenics</subject><subject>Antineoplastic agents</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>Cornus - chemistry</subject><subject>Cornus kousa</subject><subject>Diabetes</subject><subject>Down-Regulation - drug effects</subject><subject>Ethanol</subject><subject>Fatty acids</subject><subject>Food</subject><subject>Functional foods</subject><subject>Functional foods & nutraceuticals</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Health aspects</subject><subject>Herbal medicine</subject><subject>High performance liquid chromatography</subject><subject>Human Umbilical Vein Endothelial Cells</subject><subject>Humans</subject><subject>Kinases</subject><subject>Leaves</subject><subject>Lipids</subject><subject>Lipogenesis</subject><subject>Lipogenesis - drug effects</subject><subject>Liquid chromatography</subject><subject>Medicine and Health Sciences</subject><subject>Metabolic disorders</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Natural products</subject><subject>NIH 3T3 Cells</subject><subject>Obesity</subject><subject>Obesity - drug therapy</subject><subject>Physiology</subject><subject>Phytochemicals</subject><subject>Plant cells</subject><subject>Plant extracts</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Preadipocytes</subject><subject>Proteins</subject><subject>Research and analysis methods</subject><subject>Signaling</subject><subject>Weight control</subject><subject>Western blotting</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk91u1DAQhSMEoqXwBggsISG4yGLHsZPcIK0qfipVqgSFW2tiT7IuWXuJnZZ9LN4Qh26rLuoFykVi5zsn8ZmZLHvO6ILxir278NPoYFhsvMMFLWgthHyQHbKGF7ksKH945_kgexLCBaWC11I-zg44FQWTVBxmv5currzegrMukG70a3LsRzcF8sNPAQjGFTg_WE3wVxxBRwIxopsgIvEtBhu3xDoCIXhtIVrvyJWNKwIu2hxcb32PLqmT0l7aaDHMOD_n-SkjGochkHZLjL9y-Yj9NCQL1xMwdjMLMSQenCHDzdqGp9mjDoaAz3b3o-zbxw_nx5_z07NPJ8fL01zLpoi5rCV0XBveSoqMl1LQigpR17zBEqiQLYMOKOdosKpb0YFpG5DYALZQdYYfZS-vfTeDD2qXdlAFE4w1shJlIk6uCePhQm1Gu4ZxqzxY9XfDj72CMVo9oEJTFbyqilJQU-rWNFVdS1HWRcmElm2bvN7vvja1azQaXUp72DPdf-PsSvX-Usmi5rJkyeDNzmD0PycMUa1tmAMGh6mS83-nbmCiqhL66h_0_tPtqB7SAazr_Fz_2VQthSxF2QgpE7W4h0qXwbXVqTU7m_b3BG_3BImJqbV6mEJQJ1-__D979n2ffX2HXSEMcRX8MM0tGfbB8hrUow9hxO42ZEbVPFk3aah5stRuspLsxd0C3YpuRon_ARwcIlU</recordid><startdate>20181206</startdate><enddate>20181206</enddate><creator>Khan, Muhammad Imran</creator><creator>Shin, Jin Hyuk</creator><creator>Shin, Tai Sun</creator><creator>Kim, Min Yong</creator><creator>Cho, Nam Jun</creator><creator>Kim, Jong Deog</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1096-1529</orcidid></search><sort><creationdate>20181206</creationdate><title>Anthocyanins from Cornus kousa ethanolic extract attenuate obesity in association with anti-angiogenic activities in 3T3-L1 cells by down-regulating adipogeneses and lipogenesis</title><author>Khan, Muhammad Imran ; Shin, Jin Hyuk ; Shin, Tai Sun ; Kim, Min Yong ; Cho, Nam Jun ; Kim, Jong Deog</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-686af3cd3b60e13465070558839e4a056b1afa033ede78b5fadb9a6e9aeba7fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>Accumulation</topic><topic>Adipocytes</topic><topic>Adipogenesis</topic><topic>Adipogenesis - drug effects</topic><topic>AKT1 protein</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Anthocyanins</topic><topic>Anthocyanins - isolation & purification</topic><topic>Anthocyanins - pharmacology</topic><topic>Antiangiogenics</topic><topic>Antineoplastic agents</topic><topic>Biology and Life Sciences</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>Cornus - chemistry</topic><topic>Cornus kousa</topic><topic>Diabetes</topic><topic>Down-Regulation - drug effects</topic><topic>Ethanol</topic><topic>Fatty acids</topic><topic>Food</topic><topic>Functional foods</topic><topic>Functional foods & nutraceuticals</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Health aspects</topic><topic>Herbal medicine</topic><topic>High performance liquid chromatography</topic><topic>Human Umbilical Vein Endothelial Cells</topic><topic>Humans</topic><topic>Kinases</topic><topic>Leaves</topic><topic>Lipids</topic><topic>Lipogenesis</topic><topic>Lipogenesis - drug effects</topic><topic>Liquid chromatography</topic><topic>Medicine and Health Sciences</topic><topic>Metabolic disorders</topic><topic>Metastasis</topic><topic>Mice</topic><topic>Natural products</topic><topic>NIH 3T3 Cells</topic><topic>Obesity</topic><topic>Obesity - drug therapy</topic><topic>Physiology</topic><topic>Phytochemicals</topic><topic>Plant cells</topic><topic>Plant extracts</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Preadipocytes</topic><topic>Proteins</topic><topic>Research and analysis methods</topic><topic>Signaling</topic><topic>Weight control</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khan, Muhammad Imran</creatorcontrib><creatorcontrib>Shin, Jin Hyuk</creatorcontrib><creatorcontrib>Shin, Tai Sun</creatorcontrib><creatorcontrib>Kim, Min Yong</creatorcontrib><creatorcontrib>Cho, Nam Jun</creatorcontrib><creatorcontrib>Kim, Jong Deog</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khan, Muhammad Imran</au><au>Shin, Jin Hyuk</au><au>Shin, Tai Sun</au><au>Kim, Min Yong</au><au>Cho, Nam Jun</au><au>Kim, Jong Deog</au><au>López Lluch, Guillermo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anthocyanins from Cornus kousa ethanolic extract attenuate obesity in association with anti-angiogenic activities in 3T3-L1 cells by down-regulating adipogeneses and lipogenesis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-12-06</date><risdate>2018</risdate><volume>13</volume><issue>12</issue><spage>e0208556</spage><epage>e0208556</epage><pages>e0208556-e0208556</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cornus kousa the Korean dogwood has been traditionally used in East Asia as therapeutic traditional medicine however biological activities of Cornus kousa have not been investigated previously. The aim of the present study was to evaluate anti-obesity activities coupled with anti-angiogenic activities of anthocyanins rich fraction of ethanolic leaf extract of Cornus kousa (ELECk) in HUVECs and 3T3- L1 cells. Dried plants leaves were extracted with 70% ethanol and anthocyanin fraction (AnT Fr) was obtained by eluting the ethanolic extract through non-polar macroporous resin and further purification by HPLC. Antiangiogenic activities were determined by antiproliferative effect of AnT Fr on HUVECs. In the presence of various concentrations of AnT Fr, 3T3-L1 preadipocytes were induced to differentiate. Lipid accumulation in differentiated adipocytes were quantified by Oil-Red O staining. AnT Fr significantly suppressed angiogenesis by inhibiting proliferation and tube formation of HUVECs via downregulating VEGRF 2, PI3K, β-catenin, NF-kB, and Akt1 in a dose dependent manner. AnT Fr inhibited lipid accumulation by down-regulating adipogenesis and lipogenesis promoting signaling proteins, PPARγ, CCAAT, C/EBPα, aP2, FAS, and LPL, however enhanced AMPK activation to p-AMPK in 3T3 cells quantified and expressed by western blotting. AnT Fr inhibit lipid accumulation by regulating adipogenesis and lipogenesis related genes and signaling proteins. The anti-obesity activities exerted by Cornus kousa are associated with antiangiogenic activities of anthocyanins rich fraction of Cornus kousa. Hence the presence of bioactive anthocyanins, Cornus kosa, is a good candidate for nutraceutical and pharmaceutical formulation for treating or controlling obesity.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30521605</pmid><doi>10.1371/journal.pone.0208556</doi><tpages>e0208556</tpages><orcidid>https://orcid.org/0000-0003-1096-1529</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-12, Vol.13 (12), p.e0208556-e0208556 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2151196754 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 1-Phosphatidylinositol 3-kinase Accumulation Adipocytes Adipogenesis Adipogenesis - drug effects AKT1 protein Angiogenesis Animals Anthocyanins Anthocyanins - isolation & purification Anthocyanins - pharmacology Antiangiogenics Antineoplastic agents Biology and Life Sciences Biotechnology Cancer Cornus - chemistry Cornus kousa Diabetes Down-Regulation - drug effects Ethanol Fatty acids Food Functional foods Functional foods & nutraceuticals Gene expression Genes Health aspects Herbal medicine High performance liquid chromatography Human Umbilical Vein Endothelial Cells Humans Kinases Leaves Lipids Lipogenesis Lipogenesis - drug effects Liquid chromatography Medicine and Health Sciences Metabolic disorders Metastasis Mice Natural products NIH 3T3 Cells Obesity Obesity - drug therapy Physiology Phytochemicals Plant cells Plant extracts Plant Extracts - isolation & purification Plant Extracts - pharmacology Preadipocytes Proteins Research and analysis methods Signaling Weight control Western blotting |
| title | Anthocyanins from Cornus kousa ethanolic extract attenuate obesity in association with anti-angiogenic activities in 3T3-L1 cells by down-regulating adipogeneses and lipogenesis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T12%3A15%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Anthocyanins%20from%20Cornus%20kousa%20ethanolic%20extract%20attenuate%20obesity%20in%20association%20with%20anti-angiogenic%20activities%20in%203T3-L1%20cells%20by%20down-regulating%20adipogeneses%20and%20lipogenesis&rft.jtitle=PloS%20one&rft.au=Khan,%20Muhammad%20Imran&rft.date=2018-12-06&rft.volume=13&rft.issue=12&rft.spage=e0208556&rft.epage=e0208556&rft.pages=e0208556-e0208556&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0208556&rft_dat=%3Cgale_plos_%3EA564549566%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2151196754&rft_id=info:pmid/30521605&rft_galeid=A564549566&rft_doaj_id=oai_doaj_org_article_ed723772450d4cbd978865482415c6bb&rfr_iscdi=true |