Differential detection of tuberculous and non-tuberculous mycobacteria by qPCR in lavage fluids of tuberculosis-suspicious white rhinoceros
Tuberculosis (TB) occurs in a wide range of mammalian species and thus poses a health risk to humans living or working in close proximity with TB infected animals. Despite a high incidence of M. bovis infections in domestic or wildlife species tuberculosis infections in rhinoceros have so far been v...
Gespeichert in:
| Veröffentlicht in: | PloS one 2018-11, Vol.13 (11), p.e0207365 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 11 |
| container_start_page | e0207365 |
| container_title | PloS one |
| container_volume | 13 |
| creator | Hermes, Robert Saragusty, Joseph Moser, Irmgard Barth, Stefanie A Holtze, Susanne Lecu, Alexis Cracknell, Jonathan Williams, Duncan Göritz, Frank Hildebrandt, Thomas Bernd |
| description | Tuberculosis (TB) occurs in a wide range of mammalian species and thus poses a health risk to humans living or working in close proximity with TB infected animals. Despite a high incidence of M. bovis infections in domestic or wildlife species tuberculosis infections in rhinoceros have so far been very limited. Over the past 53 years, tuberculosis of the respiratory tract has been confirmed in just 22 rhinoceros, most of those infected not by M. bovis but M. tuberculosis. However, because of the zoonotic risk TB testing is recommended or becomes even mandatory in endangered species. The dilemma in rhinoceros and many other wildlife species; non-validated tests are highly inconsistent in their ability to identify TB infection. Current lack of TB diagnostics may result in TB positive rhinoceros living with the infection, transmitting it to those around them or in euthanasia of animals found unconfirmed at necropsy. This is an unacceptable diagnostic status considering that some species are critically endangered and therefore should not be euthanized in order to confirm suspicion of disease. To overcome this shortcoming we used bronchoscopy to detect mycobacteria in respiratory fluids of TB suspicious rhinoceros. Fluids from seven, TB suspicious white rhinoceros were harvested during 21 bronchoscopies. Our new approach: In addition to bacterial culture a dual quantitative PCR system tested for the general presence of DNA from NTM and more specifically for DNA from MTC. Both, bacterial culture and qPCR were negative for MTC in respiratory fluids of all rhinoceros (7/7). At the same time, respiratory fluids from six rhinoceros tested positive for the presence of NTM or other closely related bacteria (6/7). M. tuberculosis was found only once in an oesophageal aspirate. The high incidence of mycobacterial DNA in the respiratory tract suggests that white rhinoceros, as strict grazers, are immensely exposed to environmental bacteria of this genus. Presence of NTM in the respiratory or intestinal system could possibly cause false positive results in intradermal tests. A wider use of bronchoalveolar lavage is warranted to further elucidate immunologic response to NTM and exposure to, incidence and prevalence of MTC infections in rhinoceros. |
| doi_str_mv | 10.1371/journal.pone.0207365 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2139104138</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A563638757</galeid><doaj_id>oai_doaj_org_article_e1cda2a3a704426381a210698c5c96bf</doaj_id><sourcerecordid>A563638757</sourcerecordid><originalsourceid>FETCH-LOGICAL-c641t-10f23bd94d55531d76bd7b9f6b9207cb6b40b27e2ce13384bed54001adbccf0d3</originalsourceid><addsrcrecordid>eNqNk2uL1DAUhoso7rr6D0QDguCHjrm0aftlYRlvAwsr6-VryHUmQyeZTdLV-Q3-aTNOd5mCguRDw-lz3ry8nFMUzxGcIdKgt2s_BMf72dY7PYMYNoTWD4pT1BFcUgzJw6P7SfEkxjWENWkpfVycEFi1NWnwafHrnTVGB-2S5T1QOmmZrHfAG5AGoYMcej9EwJ0CzrvyuLbZSS-4TDpYDsQO3HyeXwPrQM9v-VID0w9WxYlQtLGMQ9xaafcCP1Y2aRBW1nmpg49Pi0eG91E_G79nxbcP77_OP5WXVx8X84vLUtIKpRJBg4lQXaXquiZINVSoRnSGii6nIAUVFRS40VhqREhbCa3qCkLElZDSQEXOipcH3W22xMYcI8OIdAhWiLSZWBwI5fmabYPd8LBjnlv2p-DDkvGQrOw100gqjjnhDawqTEmLOEaQdq2sZUeFyVrn42uD2Gglc9SB9xPR6R9nV2zpbxnFFNUNzAKvRoHgbwYd0z8sj9SSZ1fWGZ_F5MZGyS5qSrKxpm4yNfsLlY_SGyvzJBmb65OGN5OGzCT9My35ECNbfLn-f_bq-5R9fcSuNO_TKvp-2A9fnILVAZR5QmLQ5j45BNl-Ee7SYPtFYOMi5LYXx6nfN91NPvkNlrsG-Q</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2139104138</pqid></control><display><type>article</type><title>Differential detection of tuberculous and non-tuberculous mycobacteria by qPCR in lavage fluids of tuberculosis-suspicious white rhinoceros</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Hermes, Robert ; Saragusty, Joseph ; Moser, Irmgard ; Barth, Stefanie A ; Holtze, Susanne ; Lecu, Alexis ; Cracknell, Jonathan ; Williams, Duncan ; Göritz, Frank ; Hildebrandt, Thomas Bernd</creator><contributor>Spigelman, Mark</contributor><creatorcontrib>Hermes, Robert ; Saragusty, Joseph ; Moser, Irmgard ; Barth, Stefanie A ; Holtze, Susanne ; Lecu, Alexis ; Cracknell, Jonathan ; Williams, Duncan ; Göritz, Frank ; Hildebrandt, Thomas Bernd ; Spigelman, Mark</creatorcontrib><description>Tuberculosis (TB) occurs in a wide range of mammalian species and thus poses a health risk to humans living or working in close proximity with TB infected animals. Despite a high incidence of M. bovis infections in domestic or wildlife species tuberculosis infections in rhinoceros have so far been very limited. Over the past 53 years, tuberculosis of the respiratory tract has been confirmed in just 22 rhinoceros, most of those infected not by M. bovis but M. tuberculosis. However, because of the zoonotic risk TB testing is recommended or becomes even mandatory in endangered species. The dilemma in rhinoceros and many other wildlife species; non-validated tests are highly inconsistent in their ability to identify TB infection. Current lack of TB diagnostics may result in TB positive rhinoceros living with the infection, transmitting it to those around them or in euthanasia of animals found unconfirmed at necropsy. This is an unacceptable diagnostic status considering that some species are critically endangered and therefore should not be euthanized in order to confirm suspicion of disease. To overcome this shortcoming we used bronchoscopy to detect mycobacteria in respiratory fluids of TB suspicious rhinoceros. Fluids from seven, TB suspicious white rhinoceros were harvested during 21 bronchoscopies. Our new approach: In addition to bacterial culture a dual quantitative PCR system tested for the general presence of DNA from NTM and more specifically for DNA from MTC. Both, bacterial culture and qPCR were negative for MTC in respiratory fluids of all rhinoceros (7/7). At the same time, respiratory fluids from six rhinoceros tested positive for the presence of NTM or other closely related bacteria (6/7). M. tuberculosis was found only once in an oesophageal aspirate. The high incidence of mycobacterial DNA in the respiratory tract suggests that white rhinoceros, as strict grazers, are immensely exposed to environmental bacteria of this genus. Presence of NTM in the respiratory or intestinal system could possibly cause false positive results in intradermal tests. A wider use of bronchoalveolar lavage is warranted to further elucidate immunologic response to NTM and exposure to, incidence and prevalence of MTC infections in rhinoceros.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0207365</identifier><identifier>PMID: 30485372</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Alveoli ; Animals ; Antigens ; Bacteria ; Beef cattle ; Biology and Life Sciences ; Bronchoalveolar Lavage ; Bronchoscopy ; Bronchus ; Cattle ; Ceratotherium simum ; Deoxyribonucleic acid ; Diagnosis ; Diagnostic systems ; Disease transmission ; DNA ; Endangered species ; Esophagus ; Euthanasia ; Fluids ; Health aspects ; Health risks ; Incidence ; Infections ; Intestine ; Mammals ; Medical diagnosis ; Medicine and Health Sciences ; Mycobacterium bovis - genetics ; Mycobacterium tuberculosis - genetics ; Necropsy ; Occupational health ; Pathogenesis ; Polymerase chain reaction ; Prevalence ; Prevalence studies (Epidemiology) ; Real-Time Polymerase Chain Reaction ; Research and Analysis Methods ; Respiratory tract ; Risk factors ; Tuberculosis ; Tuberculosis, Pulmonary - diagnosis ; Tuberculosis, Pulmonary - genetics ; Tuberculosis, Pulmonary - veterinary ; Veterinary medicine ; White rhinoceros ; Wildlife ; Wildlife conservation ; Zoonoses</subject><ispartof>PloS one, 2018-11, Vol.13 (11), p.e0207365</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Hermes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Hermes et al 2018 Hermes et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c641t-10f23bd94d55531d76bd7b9f6b9207cb6b40b27e2ce13384bed54001adbccf0d3</cites><orcidid>0000-0002-9274-7800 ; 0000-0003-2487-9171</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261570/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261570/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23864,27922,27923,53789,53791,79370,79371</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30485372$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Spigelman, Mark</contributor><creatorcontrib>Hermes, Robert</creatorcontrib><creatorcontrib>Saragusty, Joseph</creatorcontrib><creatorcontrib>Moser, Irmgard</creatorcontrib><creatorcontrib>Barth, Stefanie A</creatorcontrib><creatorcontrib>Holtze, Susanne</creatorcontrib><creatorcontrib>Lecu, Alexis</creatorcontrib><creatorcontrib>Cracknell, Jonathan</creatorcontrib><creatorcontrib>Williams, Duncan</creatorcontrib><creatorcontrib>Göritz, Frank</creatorcontrib><creatorcontrib>Hildebrandt, Thomas Bernd</creatorcontrib><title>Differential detection of tuberculous and non-tuberculous mycobacteria by qPCR in lavage fluids of tuberculosis-suspicious white rhinoceros</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Tuberculosis (TB) occurs in a wide range of mammalian species and thus poses a health risk to humans living or working in close proximity with TB infected animals. Despite a high incidence of M. bovis infections in domestic or wildlife species tuberculosis infections in rhinoceros have so far been very limited. Over the past 53 years, tuberculosis of the respiratory tract has been confirmed in just 22 rhinoceros, most of those infected not by M. bovis but M. tuberculosis. However, because of the zoonotic risk TB testing is recommended or becomes even mandatory in endangered species. The dilemma in rhinoceros and many other wildlife species; non-validated tests are highly inconsistent in their ability to identify TB infection. Current lack of TB diagnostics may result in TB positive rhinoceros living with the infection, transmitting it to those around them or in euthanasia of animals found unconfirmed at necropsy. This is an unacceptable diagnostic status considering that some species are critically endangered and therefore should not be euthanized in order to confirm suspicion of disease. To overcome this shortcoming we used bronchoscopy to detect mycobacteria in respiratory fluids of TB suspicious rhinoceros. Fluids from seven, TB suspicious white rhinoceros were harvested during 21 bronchoscopies. Our new approach: In addition to bacterial culture a dual quantitative PCR system tested for the general presence of DNA from NTM and more specifically for DNA from MTC. Both, bacterial culture and qPCR were negative for MTC in respiratory fluids of all rhinoceros (7/7). At the same time, respiratory fluids from six rhinoceros tested positive for the presence of NTM or other closely related bacteria (6/7). M. tuberculosis was found only once in an oesophageal aspirate. The high incidence of mycobacterial DNA in the respiratory tract suggests that white rhinoceros, as strict grazers, are immensely exposed to environmental bacteria of this genus. Presence of NTM in the respiratory or intestinal system could possibly cause false positive results in intradermal tests. A wider use of bronchoalveolar lavage is warranted to further elucidate immunologic response to NTM and exposure to, incidence and prevalence of MTC infections in rhinoceros.</description><subject>Alveoli</subject><subject>Animals</subject><subject>Antigens</subject><subject>Bacteria</subject><subject>Beef cattle</subject><subject>Biology and Life Sciences</subject><subject>Bronchoalveolar Lavage</subject><subject>Bronchoscopy</subject><subject>Bronchus</subject><subject>Cattle</subject><subject>Ceratotherium simum</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>Disease transmission</subject><subject>DNA</subject><subject>Endangered species</subject><subject>Esophagus</subject><subject>Euthanasia</subject><subject>Fluids</subject><subject>Health aspects</subject><subject>Health risks</subject><subject>Incidence</subject><subject>Infections</subject><subject>Intestine</subject><subject>Mammals</subject><subject>Medical diagnosis</subject><subject>Medicine and Health Sciences</subject><subject>Mycobacterium bovis - genetics</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Necropsy</subject><subject>Occupational health</subject><subject>Pathogenesis</subject><subject>Polymerase chain reaction</subject><subject>Prevalence</subject><subject>Prevalence studies (Epidemiology)</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Research and Analysis Methods</subject><subject>Respiratory tract</subject><subject>Risk factors</subject><subject>Tuberculosis</subject><subject>Tuberculosis, Pulmonary - diagnosis</subject><subject>Tuberculosis, Pulmonary - genetics</subject><subject>Tuberculosis, Pulmonary - veterinary</subject><subject>Veterinary medicine</subject><subject>White rhinoceros</subject><subject>Wildlife</subject><subject>Wildlife conservation</subject><subject>Zoonoses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk2uL1DAUhoso7rr6D0QDguCHjrm0aftlYRlvAwsr6-VryHUmQyeZTdLV-Q3-aTNOd5mCguRDw-lz3ry8nFMUzxGcIdKgt2s_BMf72dY7PYMYNoTWD4pT1BFcUgzJw6P7SfEkxjWENWkpfVycEFi1NWnwafHrnTVGB-2S5T1QOmmZrHfAG5AGoYMcej9EwJ0CzrvyuLbZSS-4TDpYDsQO3HyeXwPrQM9v-VID0w9WxYlQtLGMQ9xaafcCP1Y2aRBW1nmpg49Pi0eG91E_G79nxbcP77_OP5WXVx8X84vLUtIKpRJBg4lQXaXquiZINVSoRnSGii6nIAUVFRS40VhqREhbCa3qCkLElZDSQEXOipcH3W22xMYcI8OIdAhWiLSZWBwI5fmabYPd8LBjnlv2p-DDkvGQrOw100gqjjnhDawqTEmLOEaQdq2sZUeFyVrn42uD2Gglc9SB9xPR6R9nV2zpbxnFFNUNzAKvRoHgbwYd0z8sj9SSZ1fWGZ_F5MZGyS5qSrKxpm4yNfsLlY_SGyvzJBmb65OGN5OGzCT9My35ECNbfLn-f_bq-5R9fcSuNO_TKvp-2A9fnILVAZR5QmLQ5j45BNl-Ee7SYPtFYOMi5LYXx6nfN91NPvkNlrsG-Q</recordid><startdate>20181128</startdate><enddate>20181128</enddate><creator>Hermes, Robert</creator><creator>Saragusty, Joseph</creator><creator>Moser, Irmgard</creator><creator>Barth, Stefanie A</creator><creator>Holtze, Susanne</creator><creator>Lecu, Alexis</creator><creator>Cracknell, Jonathan</creator><creator>Williams, Duncan</creator><creator>Göritz, Frank</creator><creator>Hildebrandt, Thomas Bernd</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-9274-7800</orcidid><orcidid>https://orcid.org/0000-0003-2487-9171</orcidid></search><sort><creationdate>20181128</creationdate><title>Differential detection of tuberculous and non-tuberculous mycobacteria by qPCR in lavage fluids of tuberculosis-suspicious white rhinoceros</title><author>Hermes, Robert ; Saragusty, Joseph ; Moser, Irmgard ; Barth, Stefanie A ; Holtze, Susanne ; Lecu, Alexis ; Cracknell, Jonathan ; Williams, Duncan ; Göritz, Frank ; Hildebrandt, Thomas Bernd</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c641t-10f23bd94d55531d76bd7b9f6b9207cb6b40b27e2ce13384bed54001adbccf0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alveoli</topic><topic>Animals</topic><topic>Antigens</topic><topic>Bacteria</topic><topic>Beef cattle</topic><topic>Biology and Life Sciences</topic><topic>Bronchoalveolar Lavage</topic><topic>Bronchoscopy</topic><topic>Bronchus</topic><topic>Cattle</topic><topic>Ceratotherium simum</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnosis</topic><topic>Diagnostic systems</topic><topic>Disease transmission</topic><topic>DNA</topic><topic>Endangered species</topic><topic>Esophagus</topic><topic>Euthanasia</topic><topic>Fluids</topic><topic>Health aspects</topic><topic>Health risks</topic><topic>Incidence</topic><topic>Infections</topic><topic>Intestine</topic><topic>Mammals</topic><topic>Medical diagnosis</topic><topic>Medicine and Health Sciences</topic><topic>Mycobacterium bovis - genetics</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Necropsy</topic><topic>Occupational health</topic><topic>Pathogenesis</topic><topic>Polymerase chain reaction</topic><topic>Prevalence</topic><topic>Prevalence studies (Epidemiology)</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Research and Analysis Methods</topic><topic>Respiratory tract</topic><topic>Risk factors</topic><topic>Tuberculosis</topic><topic>Tuberculosis, Pulmonary - diagnosis</topic><topic>Tuberculosis, Pulmonary - genetics</topic><topic>Tuberculosis, Pulmonary - veterinary</topic><topic>Veterinary medicine</topic><topic>White rhinoceros</topic><topic>Wildlife</topic><topic>Wildlife conservation</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hermes, Robert</creatorcontrib><creatorcontrib>Saragusty, Joseph</creatorcontrib><creatorcontrib>Moser, Irmgard</creatorcontrib><creatorcontrib>Barth, Stefanie A</creatorcontrib><creatorcontrib>Holtze, Susanne</creatorcontrib><creatorcontrib>Lecu, Alexis</creatorcontrib><creatorcontrib>Cracknell, Jonathan</creatorcontrib><creatorcontrib>Williams, Duncan</creatorcontrib><creatorcontrib>Göritz, Frank</creatorcontrib><creatorcontrib>Hildebrandt, Thomas Bernd</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hermes, Robert</au><au>Saragusty, Joseph</au><au>Moser, Irmgard</au><au>Barth, Stefanie A</au><au>Holtze, Susanne</au><au>Lecu, Alexis</au><au>Cracknell, Jonathan</au><au>Williams, Duncan</au><au>Göritz, Frank</au><au>Hildebrandt, Thomas Bernd</au><au>Spigelman, Mark</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential detection of tuberculous and non-tuberculous mycobacteria by qPCR in lavage fluids of tuberculosis-suspicious white rhinoceros</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-11-28</date><risdate>2018</risdate><volume>13</volume><issue>11</issue><spage>e0207365</spage><pages>e0207365-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Tuberculosis (TB) occurs in a wide range of mammalian species and thus poses a health risk to humans living or working in close proximity with TB infected animals. Despite a high incidence of M. bovis infections in domestic or wildlife species tuberculosis infections in rhinoceros have so far been very limited. Over the past 53 years, tuberculosis of the respiratory tract has been confirmed in just 22 rhinoceros, most of those infected not by M. bovis but M. tuberculosis. However, because of the zoonotic risk TB testing is recommended or becomes even mandatory in endangered species. The dilemma in rhinoceros and many other wildlife species; non-validated tests are highly inconsistent in their ability to identify TB infection. Current lack of TB diagnostics may result in TB positive rhinoceros living with the infection, transmitting it to those around them or in euthanasia of animals found unconfirmed at necropsy. This is an unacceptable diagnostic status considering that some species are critically endangered and therefore should not be euthanized in order to confirm suspicion of disease. To overcome this shortcoming we used bronchoscopy to detect mycobacteria in respiratory fluids of TB suspicious rhinoceros. Fluids from seven, TB suspicious white rhinoceros were harvested during 21 bronchoscopies. Our new approach: In addition to bacterial culture a dual quantitative PCR system tested for the general presence of DNA from NTM and more specifically for DNA from MTC. Both, bacterial culture and qPCR were negative for MTC in respiratory fluids of all rhinoceros (7/7). At the same time, respiratory fluids from six rhinoceros tested positive for the presence of NTM or other closely related bacteria (6/7). M. tuberculosis was found only once in an oesophageal aspirate. The high incidence of mycobacterial DNA in the respiratory tract suggests that white rhinoceros, as strict grazers, are immensely exposed to environmental bacteria of this genus. Presence of NTM in the respiratory or intestinal system could possibly cause false positive results in intradermal tests. A wider use of bronchoalveolar lavage is warranted to further elucidate immunologic response to NTM and exposure to, incidence and prevalence of MTC infections in rhinoceros.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30485372</pmid><doi>10.1371/journal.pone.0207365</doi><tpages>e0207365</tpages><orcidid>https://orcid.org/0000-0002-9274-7800</orcidid><orcidid>https://orcid.org/0000-0003-2487-9171</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-11, Vol.13 (11), p.e0207365 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2139104138 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Alveoli Animals Antigens Bacteria Beef cattle Biology and Life Sciences Bronchoalveolar Lavage Bronchoscopy Bronchus Cattle Ceratotherium simum Deoxyribonucleic acid Diagnosis Diagnostic systems Disease transmission DNA Endangered species Esophagus Euthanasia Fluids Health aspects Health risks Incidence Infections Intestine Mammals Medical diagnosis Medicine and Health Sciences Mycobacterium bovis - genetics Mycobacterium tuberculosis - genetics Necropsy Occupational health Pathogenesis Polymerase chain reaction Prevalence Prevalence studies (Epidemiology) Real-Time Polymerase Chain Reaction Research and Analysis Methods Respiratory tract Risk factors Tuberculosis Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - genetics Tuberculosis, Pulmonary - veterinary Veterinary medicine White rhinoceros Wildlife Wildlife conservation Zoonoses |
| title | Differential detection of tuberculous and non-tuberculous mycobacteria by qPCR in lavage fluids of tuberculosis-suspicious white rhinoceros |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T15%3A26%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20detection%20of%20tuberculous%20and%20non-tuberculous%20mycobacteria%20by%20qPCR%20in%20lavage%20fluids%20of%20tuberculosis-suspicious%20white%20rhinoceros&rft.jtitle=PloS%20one&rft.au=Hermes,%20Robert&rft.date=2018-11-28&rft.volume=13&rft.issue=11&rft.spage=e0207365&rft.pages=e0207365-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0207365&rft_dat=%3Cgale_plos_%3EA563638757%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2139104138&rft_id=info:pmid/30485372&rft_galeid=A563638757&rft_doaj_id=oai_doaj_org_article_e1cda2a3a704426381a210698c5c96bf&rfr_iscdi=true |