DNA barcodes corroborating identification of mosquito species and multiplex real-time PCR differentiating Culex pipiens complex and Culex torrentium in Iran
Identifying mosquito species is a fundamental step in risk assessment and implementation of preventative strategies. Moreover, Culex pipiens is the most widespread mosquito vector in several regions of Iran and is the main vector for transmission of West Nile virus (WNV). Mosquitoes were collected a...
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| creator | Shahhosseini, Nariman Kayedi, Mohammad Hassan Sedaghat, Mohammad Mehdi Racine, Trina P Kobinger, Gary Moosa-Kazemi, Seyed Hassan |
| description | Identifying mosquito species is a fundamental step in risk assessment and implementation of preventative strategies. Moreover, Culex pipiens is the most widespread mosquito vector in several regions of Iran and is the main vector for transmission of West Nile virus (WNV). Mosquitoes were collected at 14 sites in northern regions of Iran in 2015 and 2016. A subset of mosquito specimens was selected for identification confirmation using a DNA-barcoding technique. Construction of a phylogenetic tree showed clustering of mosquito sequences into three main genera: Aedes, Anopheles and Culex with individuals of a single species clustered closely together, regardless of where and when they were collected. Cx. pipiens complex and Cx. torrentium were identified and differentiated using multiplex real-time PCR targeting the gene locus for acetylcholinesterase 2 (ace2) to discriminate between Cx. pipiens pipiens and Cx. torrentium. The CQ11 microsatellite locus was used for discrimination between Cpp. biotypes. The predominant mosquito species in investigated regions were Cx. pipiens pipiens biotype pipiens, but we also detected Culex pipiens pipiens biotype molestus and hybrids of the two pipiens biotypes, as well as Cx. torrentium. The results of this study represent the first certain evidence of the presence of Cx. pipiens pipiens biotype molestus and hybrids between pipiens and molestus forms, and Cx. torrentium in Iran through a molecular identification approach. This report of a potentially important bridge vector for WNV might have key influence in the risk projections for WNV in Iran. |
| doi_str_mv | 10.1371/journal.pone.0207308 |
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Moreover, Culex pipiens is the most widespread mosquito vector in several regions of Iran and is the main vector for transmission of West Nile virus (WNV). Mosquitoes were collected at 14 sites in northern regions of Iran in 2015 and 2016. A subset of mosquito specimens was selected for identification confirmation using a DNA-barcoding technique. Construction of a phylogenetic tree showed clustering of mosquito sequences into three main genera: Aedes, Anopheles and Culex with individuals of a single species clustered closely together, regardless of where and when they were collected. Cx. pipiens complex and Cx. torrentium were identified and differentiated using multiplex real-time PCR targeting the gene locus for acetylcholinesterase 2 (ace2) to discriminate between Cx. pipiens pipiens and Cx. torrentium. The CQ11 microsatellite locus was used for discrimination between Cpp. biotypes. The predominant mosquito species in investigated regions were Cx. pipiens pipiens biotype pipiens, but we also detected Culex pipiens pipiens biotype molestus and hybrids of the two pipiens biotypes, as well as Cx. torrentium. The results of this study represent the first certain evidence of the presence of Cx. pipiens pipiens biotype molestus and hybrids between pipiens and molestus forms, and Cx. torrentium in Iran through a molecular identification approach. This report of a potentially important bridge vector for WNV might have key influence in the risk projections for WNV in Iran.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0207308</identifier><identifier>PMID: 30427929</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acetylcholinesterase ; Animals ; Aquatic insects ; Bar codes ; Biology and Life Sciences ; Biotypes ; Clustering ; Computer and Information Sciences ; Culex ; Culex - classification ; Culex - genetics ; Culex pipiens ; Culicidae - classification ; Culicidae - genetics ; Dengue fever ; Deoxyribonucleic acid ; Disease transmission ; DNA ; DNA Barcoding, Taxonomic - methods ; Female ; Gene sequencing ; Hybrids ; Identification ; Infectious diseases ; Insect control ; Iran ; Laboratories ; Loci ; Medicine ; Medicine and Health Sciences ; Mitochondrial DNA ; Morphology ; Mosquitoes ; Multiplex Polymerase Chain Reaction - methods ; Multiplexing ; People and Places ; Phylogenetics ; Phylogeny ; Polymerase chain reaction ; Real time ; Research and Analysis Methods ; Risk assessment ; Sequence Analysis, DNA - methods ; Social Sciences ; Species ; Tropical diseases ; Vector-borne diseases ; Viruses ; West Nile virus</subject><ispartof>PloS one, 2018-11, Vol.13 (11), p.e0207308-e0207308</ispartof><rights>2018 Shahhosseini et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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This report of a potentially important bridge vector for WNV might have key influence in the risk projections for WNV in Iran.</description><subject>Acetylcholinesterase</subject><subject>Animals</subject><subject>Aquatic insects</subject><subject>Bar codes</subject><subject>Biology and Life Sciences</subject><subject>Biotypes</subject><subject>Clustering</subject><subject>Computer and Information Sciences</subject><subject>Culex</subject><subject>Culex - classification</subject><subject>Culex - genetics</subject><subject>Culex pipiens</subject><subject>Culicidae - classification</subject><subject>Culicidae - genetics</subject><subject>Dengue fever</subject><subject>Deoxyribonucleic acid</subject><subject>Disease transmission</subject><subject>DNA</subject><subject>DNA Barcoding, Taxonomic - methods</subject><subject>Female</subject><subject>Gene sequencing</subject><subject>Hybrids</subject><subject>Identification</subject><subject>Infectious diseases</subject><subject>Insect control</subject><subject>Iran</subject><subject>Laboratories</subject><subject>Loci</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mitochondrial DNA</subject><subject>Morphology</subject><subject>Mosquitoes</subject><subject>Multiplex Polymerase Chain Reaction - methods</subject><subject>Multiplexing</subject><subject>People and Places</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Real time</subject><subject>Research and Analysis Methods</subject><subject>Risk assessment</subject><subject>Sequence Analysis, DNA - methods</subject><subject>Social Sciences</subject><subject>Species</subject><subject>Tropical diseases</subject><subject>Vector-borne diseases</subject><subject>Viruses</subject><subject>West Nile virus</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptkstu1DAUhiMEoqXwBggsdcNmBsd24niDVA23kSpACNaWYx8PHiV2aicI3oWHxWnSqkXIC9_-_zsXnaJ4XuJtSXn5-him6FW3HYKHLSaYU9w8KE5LQcmmJpg-vHM-KZ6kdMS4ok1dPy5OKGaECyJOiz9vP12gVkUdDCSkQ4yhDVGNzh-QM-BHZ53O1-BRsKgP6WpyY0BpAO2yQXmD-qkb3dDBLxRBdZvR9YC-7L4i46yFOCMW3G6aNYMbHPg5VH_tmQnLz5iDz-qpR86jfVT-afHIqi7Bs3U_K76_f_dt93Fz-fnDfndxudEVqccNVZpVVWtbbLniDdjGNA0ljag4Bq5azbixtm25ZlprRgS13DJhCUBjiajoWfFy4Q5dSHJtbJKkpJSVJHcqK_aLwgR1lEN0vYq_ZVBOXj-EeJAqjk53IAVnmrYNYNNWjPFaCKAqJ2BqUwuO68x6s0ab2h6MzjVH1d2D3v_x7oc8hJ-yJrTKKwNerYAYriZIo-xd0tB1ykOYlrwbIkrOsvT8H-n_q2OLSseQUgR7m0yJ5TxsNy45D5tchy3bXtwt5NZ0M130L9B71zM</recordid><startdate>20181114</startdate><enddate>20181114</enddate><creator>Shahhosseini, Nariman</creator><creator>Kayedi, Mohammad Hassan</creator><creator>Sedaghat, Mohammad Mehdi</creator><creator>Racine, Trina</creator><creator>P Kobinger, Gary</creator><creator>Moosa-Kazemi, Seyed Hassan</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-0921-1050</orcidid><orcidid>https://orcid.org/0000-0001-8827-7191</orcidid><orcidid>https://orcid.org/0000-0001-6370-8661</orcidid></search><sort><creationdate>20181114</creationdate><title>DNA barcodes corroborating identification of mosquito species and multiplex real-time PCR differentiating Culex pipiens complex and Culex torrentium in Iran</title><author>Shahhosseini, Nariman ; Kayedi, Mohammad Hassan ; Sedaghat, Mohammad Mehdi ; Racine, Trina ; P Kobinger, Gary ; Moosa-Kazemi, Seyed Hassan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-3ac455bfb0f7a78ef8d883289570e7abc47dffbb7c4ccc4293f7f49f2ee8f2953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acetylcholinesterase</topic><topic>Animals</topic><topic>Aquatic insects</topic><topic>Bar codes</topic><topic>Biology and Life Sciences</topic><topic>Biotypes</topic><topic>Clustering</topic><topic>Computer and Information Sciences</topic><topic>Culex</topic><topic>Culex - 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The predominant mosquito species in investigated regions were Cx. pipiens pipiens biotype pipiens, but we also detected Culex pipiens pipiens biotype molestus and hybrids of the two pipiens biotypes, as well as Cx. torrentium. The results of this study represent the first certain evidence of the presence of Cx. pipiens pipiens biotype molestus and hybrids between pipiens and molestus forms, and Cx. torrentium in Iran through a molecular identification approach. This report of a potentially important bridge vector for WNV might have key influence in the risk projections for WNV in Iran.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30427929</pmid><doi>10.1371/journal.pone.0207308</doi><orcidid>https://orcid.org/0000-0003-0921-1050</orcidid><orcidid>https://orcid.org/0000-0001-8827-7191</orcidid><orcidid>https://orcid.org/0000-0001-6370-8661</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2018-11, Vol.13 (11), p.e0207308-e0207308 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2133412279 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acetylcholinesterase Animals Aquatic insects Bar codes Biology and Life Sciences Biotypes Clustering Computer and Information Sciences Culex Culex - classification Culex - genetics Culex pipiens Culicidae - classification Culicidae - genetics Dengue fever Deoxyribonucleic acid Disease transmission DNA DNA Barcoding, Taxonomic - methods Female Gene sequencing Hybrids Identification Infectious diseases Insect control Iran Laboratories Loci Medicine Medicine and Health Sciences Mitochondrial DNA Morphology Mosquitoes Multiplex Polymerase Chain Reaction - methods Multiplexing People and Places Phylogenetics Phylogeny Polymerase chain reaction Real time Research and Analysis Methods Risk assessment Sequence Analysis, DNA - methods Social Sciences Species Tropical diseases Vector-borne diseases Viruses West Nile virus |
| title | DNA barcodes corroborating identification of mosquito species and multiplex real-time PCR differentiating Culex pipiens complex and Culex torrentium in Iran |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T13%3A26%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA%20barcodes%20corroborating%20identification%20of%20mosquito%20species%20and%20multiplex%20real-time%20PCR%20differentiating%20Culex%20pipiens%20complex%20and%20Culex%20torrentium%20in%20Iran&rft.jtitle=PloS%20one&rft.au=Shahhosseini,%20Nariman&rft.date=2018-11-14&rft.volume=13&rft.issue=11&rft.spage=e0207308&rft.epage=e0207308&rft.pages=e0207308-e0207308&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0207308&rft_dat=%3Cproquest_plos_%3E2133829174%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2133412279&rft_id=info:pmid/30427929&rft_doaj_id=oai_doaj_org_article_974c3b8e0db5447699e3a7dfd6d69706&rfr_iscdi=true |