Differential expression of mRNA-miRNAs related to intramuscular fat content in the longissimus dorsi in Xinjiang brown cattle
In this study, we examined the role of mRNAs and miRNAs in variations in intramuscular fat content in the longissimus dorsi muscle in Xinjiang brown cattle. Two groups of Xinjiang brown cattle with extremely different intramuscular fat content in the longissimus dorsi were selected for combined of m...
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| creator | Li, Na Zhang, Yang Li, Hai-Peng Han, Ling Yan, Xiang-Min Li, Hong-Bo Du, Wei Zhang, Jin-Shan Yu, Qun-Li |
| description | In this study, we examined the role of mRNAs and miRNAs in variations in intramuscular fat content in the longissimus dorsi muscle in Xinjiang brown cattle. Two groups of Xinjiang brown cattle with extremely different intramuscular fat content in the longissimus dorsi were selected for combined of miRNA and mRNA analysis using an RNA-Seq. In total, 296 mRNAs and 362 miRNAs were significantly differentially expressed, including 155 newly predicted miRNAs, 275 significantly upregulated genes, 252 significantly upregulated miRNAs, 21 significantly downregulated genes and 110 significantly downregulated miRNAs. The combined miRNA and mRNA analysis identified 96 differentially expressed miRNAs and 27 differentially expressed mRNAs. In all, 47 upregulated miRNAs had a regulatory effect on 14 differentially downregulated target genes, and 49 downregulated miRNAs had a regulatory effect on 13 upregulated target genes. To verify the sequencing results, 10 differentially expressed genes (DEGs) and 10 differentially expressed miRNAs were selected for qRT-PCR. The qRT-PCR results confirmed the sequencing results. The results of this study shed light on the molecular regulation of bovine adipose tissue, which might help with the development of new strategies for improving meat quality and animal productivity in beef cattle to provide healthier meat products for consumers. |
| doi_str_mv | 10.1371/journal.pone.0206757 |
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Two groups of Xinjiang brown cattle with extremely different intramuscular fat content in the longissimus dorsi were selected for combined of miRNA and mRNA analysis using an RNA-Seq. In total, 296 mRNAs and 362 miRNAs were significantly differentially expressed, including 155 newly predicted miRNAs, 275 significantly upregulated genes, 252 significantly upregulated miRNAs, 21 significantly downregulated genes and 110 significantly downregulated miRNAs. The combined miRNA and mRNA analysis identified 96 differentially expressed miRNAs and 27 differentially expressed mRNAs. In all, 47 upregulated miRNAs had a regulatory effect on 14 differentially downregulated target genes, and 49 downregulated miRNAs had a regulatory effect on 13 upregulated target genes. To verify the sequencing results, 10 differentially expressed genes (DEGs) and 10 differentially expressed miRNAs were selected for qRT-PCR. The qRT-PCR results confirmed the sequencing results. The results of this study shed light on the molecular regulation of bovine adipose tissue, which might help with the development of new strategies for improving meat quality and animal productivity in beef cattle to provide healthier meat products for consumers.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0206757</identifier><identifier>PMID: 30412616</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adipocytes ; Adipose tissue ; Adipose Tissue - anatomy & histology ; Adipose Tissue - metabolism ; Animal sciences ; Animals ; Beef cattle ; Biology and life sciences ; Cattle ; Cattle - anatomy & histology ; Cattle - metabolism ; Food Quality ; Food science ; Gene expression ; Gene Expression Regulation ; Gene sequencing ; Genes ; Insulin ; Kinases ; Livestock ; Mammals ; Meat ; Meat products ; Medicine and Health Sciences ; Metabolism ; MicroRNAs ; MicroRNAs - metabolism ; miRNA ; Mitochondrial DNA ; mRNA ; Muscle, Skeletal - anatomy & histology ; Muscle, Skeletal - metabolism ; Muscles ; Research and analysis methods ; Ribonucleic acid ; RNA ; RNA, Messenger - metabolism ; Tumor necrosis factor-TNF ; Zoology</subject><ispartof>PloS one, 2018-11, Vol.13 (11), p.e0206757-e0206757</ispartof><rights>2018 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Li et al 2018 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-662058afdff6ecf119e0b592d89d9f87c9eac9175cd61398cdef2292b03818be3</citedby><cites>FETCH-LOGICAL-c526t-662058afdff6ecf119e0b592d89d9f87c9eac9175cd61398cdef2292b03818be3</cites><orcidid>0000-0001-5842-1703</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226300/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226300/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30412616$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zheng, Yun</contributor><creatorcontrib>Li, Na</creatorcontrib><creatorcontrib>Zhang, Yang</creatorcontrib><creatorcontrib>Li, Hai-Peng</creatorcontrib><creatorcontrib>Han, Ling</creatorcontrib><creatorcontrib>Yan, Xiang-Min</creatorcontrib><creatorcontrib>Li, Hong-Bo</creatorcontrib><creatorcontrib>Du, Wei</creatorcontrib><creatorcontrib>Zhang, Jin-Shan</creatorcontrib><creatorcontrib>Yu, Qun-Li</creatorcontrib><title>Differential expression of mRNA-miRNAs related to intramuscular fat content in the longissimus dorsi in Xinjiang brown cattle</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In this study, we examined the role of mRNAs and miRNAs in variations in intramuscular fat content in the longissimus dorsi muscle in Xinjiang brown cattle. Two groups of Xinjiang brown cattle with extremely different intramuscular fat content in the longissimus dorsi were selected for combined of miRNA and mRNA analysis using an RNA-Seq. In total, 296 mRNAs and 362 miRNAs were significantly differentially expressed, including 155 newly predicted miRNAs, 275 significantly upregulated genes, 252 significantly upregulated miRNAs, 21 significantly downregulated genes and 110 significantly downregulated miRNAs. The combined miRNA and mRNA analysis identified 96 differentially expressed miRNAs and 27 differentially expressed mRNAs. In all, 47 upregulated miRNAs had a regulatory effect on 14 differentially downregulated target genes, and 49 downregulated miRNAs had a regulatory effect on 13 upregulated target genes. To verify the sequencing results, 10 differentially expressed genes (DEGs) and 10 differentially expressed miRNAs were selected for qRT-PCR. The qRT-PCR results confirmed the sequencing results. The results of this study shed light on the molecular regulation of bovine adipose tissue, which might help with the development of new strategies for improving meat quality and animal productivity in beef cattle to provide healthier meat products for consumers.</description><subject>Adipocytes</subject><subject>Adipose tissue</subject><subject>Adipose Tissue - anatomy & histology</subject><subject>Adipose Tissue - metabolism</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>Beef cattle</subject><subject>Biology and life sciences</subject><subject>Cattle</subject><subject>Cattle - anatomy & histology</subject><subject>Cattle - metabolism</subject><subject>Food Quality</subject><subject>Food science</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Insulin</subject><subject>Kinases</subject><subject>Livestock</subject><subject>Mammals</subject><subject>Meat</subject><subject>Meat products</subject><subject>Medicine and Health Sciences</subject><subject>Metabolism</subject><subject>MicroRNAs</subject><subject>MicroRNAs - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Na</au><au>Zhang, Yang</au><au>Li, Hai-Peng</au><au>Han, Ling</au><au>Yan, Xiang-Min</au><au>Li, Hong-Bo</au><au>Du, Wei</au><au>Zhang, Jin-Shan</au><au>Yu, Qun-Li</au><au>Zheng, Yun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of mRNA-miRNAs related to intramuscular fat content in the longissimus dorsi in Xinjiang brown cattle</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-11-09</date><risdate>2018</risdate><volume>13</volume><issue>11</issue><spage>e0206757</spage><epage>e0206757</epage><pages>e0206757-e0206757</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In this study, we examined the role of mRNAs and miRNAs in variations in intramuscular fat content in the longissimus dorsi muscle in Xinjiang brown cattle. Two groups of Xinjiang brown cattle with extremely different intramuscular fat content in the longissimus dorsi were selected for combined of miRNA and mRNA analysis using an RNA-Seq. In total, 296 mRNAs and 362 miRNAs were significantly differentially expressed, including 155 newly predicted miRNAs, 275 significantly upregulated genes, 252 significantly upregulated miRNAs, 21 significantly downregulated genes and 110 significantly downregulated miRNAs. The combined miRNA and mRNA analysis identified 96 differentially expressed miRNAs and 27 differentially expressed mRNAs. In all, 47 upregulated miRNAs had a regulatory effect on 14 differentially downregulated target genes, and 49 downregulated miRNAs had a regulatory effect on 13 upregulated target genes. To verify the sequencing results, 10 differentially expressed genes (DEGs) and 10 differentially expressed miRNAs were selected for qRT-PCR. The qRT-PCR results confirmed the sequencing results. The results of this study shed light on the molecular regulation of bovine adipose tissue, which might help with the development of new strategies for improving meat quality and animal productivity in beef cattle to provide healthier meat products for consumers.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30412616</pmid><doi>10.1371/journal.pone.0206757</doi><orcidid>https://orcid.org/0000-0001-5842-1703</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adipocytes Adipose tissue Adipose Tissue - anatomy & histology Adipose Tissue - metabolism Animal sciences Animals Beef cattle Biology and life sciences Cattle Cattle - anatomy & histology Cattle - metabolism Food Quality Food science Gene expression Gene Expression Regulation Gene sequencing Genes Insulin Kinases Livestock Mammals Meat Meat products Medicine and Health Sciences Metabolism MicroRNAs MicroRNAs - metabolism miRNA Mitochondrial DNA mRNA Muscle, Skeletal - anatomy & histology Muscle, Skeletal - metabolism Muscles Research and analysis methods Ribonucleic acid RNA RNA, Messenger - metabolism Tumor necrosis factor-TNF Zoology |
| title | Differential expression of mRNA-miRNAs related to intramuscular fat content in the longissimus dorsi in Xinjiang brown cattle |
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