Characterization of immunogenicity of avian influenza antigens encapsulated in PLGA nanoparticles following mucosal and subcutaneous delivery in chickens
Mucosal vaccine delivery systems have paramount importance for the induction of mucosal antibody responses. Two studies were conducted to evaluate immunogenicity of inactivated AIV antigens encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs). In the first study, seven groups of...
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| creator | Alkie, Tamiru Negash Yitbarek, Alexander Taha-Abdelaziz, Khaled Astill, Jake Sharif, Shayan |
| description | Mucosal vaccine delivery systems have paramount importance for the induction of mucosal antibody responses. Two studies were conducted to evaluate immunogenicity of inactivated AIV antigens encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs). In the first study, seven groups of specific pathogen free (SPF) layer-type chickens were immunized subcutaneously at 7-days of age with different vaccine formulations followed by booster vaccinations two weeks later. Immune responses were profiled by measuring antibody (Ab) responses in sera and lachrymal secretions of vaccinated chickens. The results indicated that inactivated AIV and CpG ODN co-encapsulated in PLGA NPs (2x NanoAI+CpG) produced higher amounts of hemagglutination inhibiting antibodies compared to a group vaccinated with non-adjuvanted AIV encapsulated in PLGA NPs (NanoAI). The tested adjuvanted NPs-based vaccine (2x NanoAI+CpG) resulted in higher IgG responses in the sera and lachrymal secretions at weeks 3, 4 and 5 post-vaccination when immunized subcutaneously. The incorporation of CpG ODN led to an increase in Ab-mediated responses and was found useful to be included both in the prime and booster vaccinations. In the second study, the ability of chitosan and mannan coated PLGA NPs that encapsulated AIV and CpG ODN was evaluated for inducing antibody responses when delivered via nasal and ocular routes in one-week-old SPF layer-type chickens. These PLGA NPs-based and surface modified formulations induced robust AIV-specific antibody responses in sera and lachrymal secretions. Chitosan coated PLGA NPs resulted in the production of large quantities of lachrymal IgA and IgG compared to mannan coated NPs, which also induced detectable amounts of IgA in addition to the induction of IgG in lachrymal secretions. In both mucosal and subcutaneous vaccination approaches, although NPs delivery enhanced Ab-mediated immunity, one booster vaccination was required to generate significant amount of Abs. These results highlight the potential of NPs-based AIV antigens for promoting the induction of both systemic and mucosal immune responses against respiratory pathogens. |
| doi_str_mv | 10.1371/journal.pone.0206324 |
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Two studies were conducted to evaluate immunogenicity of inactivated AIV antigens encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs). In the first study, seven groups of specific pathogen free (SPF) layer-type chickens were immunized subcutaneously at 7-days of age with different vaccine formulations followed by booster vaccinations two weeks later. Immune responses were profiled by measuring antibody (Ab) responses in sera and lachrymal secretions of vaccinated chickens. The results indicated that inactivated AIV and CpG ODN co-encapsulated in PLGA NPs (2x NanoAI+CpG) produced higher amounts of hemagglutination inhibiting antibodies compared to a group vaccinated with non-adjuvanted AIV encapsulated in PLGA NPs (NanoAI). The tested adjuvanted NPs-based vaccine (2x NanoAI+CpG) resulted in higher IgG responses in the sera and lachrymal secretions at weeks 3, 4 and 5 post-vaccination when immunized subcutaneously. The incorporation of CpG ODN led to an increase in Ab-mediated responses and was found useful to be included both in the prime and booster vaccinations. In the second study, the ability of chitosan and mannan coated PLGA NPs that encapsulated AIV and CpG ODN was evaluated for inducing antibody responses when delivered via nasal and ocular routes in one-week-old SPF layer-type chickens. These PLGA NPs-based and surface modified formulations induced robust AIV-specific antibody responses in sera and lachrymal secretions. Chitosan coated PLGA NPs resulted in the production of large quantities of lachrymal IgA and IgG compared to mannan coated NPs, which also induced detectable amounts of IgA in addition to the induction of IgG in lachrymal secretions. In both mucosal and subcutaneous vaccination approaches, although NPs delivery enhanced Ab-mediated immunity, one booster vaccination was required to generate significant amount of Abs. These results highlight the potential of NPs-based AIV antigens for promoting the induction of both systemic and mucosal immune responses against respiratory pathogens.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0206324</identifier><identifier>PMID: 30383798</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibodies ; Antigens ; Avian flu ; Biology and Life Sciences ; Chickens ; Chitosan ; Coatings ; CpG islands ; Delivery systems ; Drug delivery systems ; Encapsulation ; Formulations ; Hemagglutination ; Immune response ; Immunity ; Immunization ; Immunogenicity ; Immunoglobulin A ; Immunoglobulin G ; Mannan ; Medicine and Health Sciences ; Mucosal immunity ; Nanoparticles ; Oligonucleotides ; Pathogens ; Polylactide-co-glycolide ; Poultry ; Respiratory diseases ; Secretions ; Specific pathogen free ; Vaccination ; Vaccines ; Veterinary colleges ; Viruses</subject><ispartof>PloS one, 2018-11, Vol.13 (11), p.e0206324-e0206324</ispartof><rights>2018 Alkie et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Two studies were conducted to evaluate immunogenicity of inactivated AIV antigens encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs). In the first study, seven groups of specific pathogen free (SPF) layer-type chickens were immunized subcutaneously at 7-days of age with different vaccine formulations followed by booster vaccinations two weeks later. Immune responses were profiled by measuring antibody (Ab) responses in sera and lachrymal secretions of vaccinated chickens. The results indicated that inactivated AIV and CpG ODN co-encapsulated in PLGA NPs (2x NanoAI+CpG) produced higher amounts of hemagglutination inhibiting antibodies compared to a group vaccinated with non-adjuvanted AIV encapsulated in PLGA NPs (NanoAI). The tested adjuvanted NPs-based vaccine (2x NanoAI+CpG) resulted in higher IgG responses in the sera and lachrymal secretions at weeks 3, 4 and 5 post-vaccination when immunized subcutaneously. 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These results highlight the potential of NPs-based AIV antigens for promoting the induction of both systemic and mucosal immune responses against respiratory pathogens.</description><subject>Antibodies</subject><subject>Antigens</subject><subject>Avian flu</subject><subject>Biology and Life Sciences</subject><subject>Chickens</subject><subject>Chitosan</subject><subject>Coatings</subject><subject>CpG islands</subject><subject>Delivery systems</subject><subject>Drug delivery systems</subject><subject>Encapsulation</subject><subject>Formulations</subject><subject>Hemagglutination</subject><subject>Immune response</subject><subject>Immunity</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Immunoglobulin A</subject><subject>Immunoglobulin G</subject><subject>Mannan</subject><subject>Medicine and Health Sciences</subject><subject>Mucosal 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of immunogenicity of avian influenza antigens encapsulated in PLGA nanoparticles following mucosal and subcutaneous delivery in chickens</title><author>Alkie, Tamiru Negash ; Yitbarek, Alexander ; Taha-Abdelaziz, Khaled ; Astill, Jake ; Sharif, Shayan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-9cd6d04edf6a409b26f01b32a214e7dfe3540dc509323945515e3f6944afe9f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Antibodies</topic><topic>Antigens</topic><topic>Avian flu</topic><topic>Biology and Life Sciences</topic><topic>Chickens</topic><topic>Chitosan</topic><topic>Coatings</topic><topic>CpG islands</topic><topic>Delivery systems</topic><topic>Drug delivery systems</topic><topic>Encapsulation</topic><topic>Formulations</topic><topic>Hemagglutination</topic><topic>Immune response</topic><topic>Immunity</topic><topic>Immunization</topic><topic>Immunogenicity</topic><topic>Immunoglobulin A</topic><topic>Immunoglobulin G</topic><topic>Mannan</topic><topic>Medicine and Health Sciences</topic><topic>Mucosal immunity</topic><topic>Nanoparticles</topic><topic>Oligonucleotides</topic><topic>Pathogens</topic><topic>Polylactide-co-glycolide</topic><topic>Poultry</topic><topic>Respiratory diseases</topic><topic>Secretions</topic><topic>Specific pathogen free</topic><topic>Vaccination</topic><topic>Vaccines</topic><topic>Veterinary colleges</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alkie, Tamiru Negash</creatorcontrib><creatorcontrib>Yitbarek, Alexander</creatorcontrib><creatorcontrib>Taha-Abdelaziz, Khaled</creatorcontrib><creatorcontrib>Astill, Jake</creatorcontrib><creatorcontrib>Sharif, 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One</addtitle><date>2018-11-01</date><risdate>2018</risdate><volume>13</volume><issue>11</issue><spage>e0206324</spage><epage>e0206324</epage><pages>e0206324-e0206324</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mucosal vaccine delivery systems have paramount importance for the induction of mucosal antibody responses. Two studies were conducted to evaluate immunogenicity of inactivated AIV antigens encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs). In the first study, seven groups of specific pathogen free (SPF) layer-type chickens were immunized subcutaneously at 7-days of age with different vaccine formulations followed by booster vaccinations two weeks later. Immune responses were profiled by measuring antibody (Ab) responses in sera and lachrymal secretions of vaccinated chickens. The results indicated that inactivated AIV and CpG ODN co-encapsulated in PLGA NPs (2x NanoAI+CpG) produced higher amounts of hemagglutination inhibiting antibodies compared to a group vaccinated with non-adjuvanted AIV encapsulated in PLGA NPs (NanoAI). The tested adjuvanted NPs-based vaccine (2x NanoAI+CpG) resulted in higher IgG responses in the sera and lachrymal secretions at weeks 3, 4 and 5 post-vaccination when immunized subcutaneously. The incorporation of CpG ODN led to an increase in Ab-mediated responses and was found useful to be included both in the prime and booster vaccinations. In the second study, the ability of chitosan and mannan coated PLGA NPs that encapsulated AIV and CpG ODN was evaluated for inducing antibody responses when delivered via nasal and ocular routes in one-week-old SPF layer-type chickens. These PLGA NPs-based and surface modified formulations induced robust AIV-specific antibody responses in sera and lachrymal secretions. Chitosan coated PLGA NPs resulted in the production of large quantities of lachrymal IgA and IgG compared to mannan coated NPs, which also induced detectable amounts of IgA in addition to the induction of IgG in lachrymal secretions. In both mucosal and subcutaneous vaccination approaches, although NPs delivery enhanced Ab-mediated immunity, one booster vaccination was required to generate significant amount of Abs. These results highlight the potential of NPs-based AIV antigens for promoting the induction of both systemic and mucosal immune responses against respiratory pathogens.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30383798</pmid><doi>10.1371/journal.pone.0206324</doi><orcidid>https://orcid.org/0000-0002-3158-012X</orcidid><orcidid>https://orcid.org/0000-0002-3500-0305</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Antigens Avian flu Biology and Life Sciences Chickens Chitosan Coatings CpG islands Delivery systems Drug delivery systems Encapsulation Formulations Hemagglutination Immune response Immunity Immunization Immunogenicity Immunoglobulin A Immunoglobulin G Mannan Medicine and Health Sciences Mucosal immunity Nanoparticles Oligonucleotides Pathogens Polylactide-co-glycolide Poultry Respiratory diseases Secretions Specific pathogen free Vaccination Vaccines Veterinary colleges Viruses |
| title | Characterization of immunogenicity of avian influenza antigens encapsulated in PLGA nanoparticles following mucosal and subcutaneous delivery in chickens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T10%3A54%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20immunogenicity%20of%20avian%20influenza%20antigens%20encapsulated%20in%20PLGA%20nanoparticles%20following%20mucosal%20and%20subcutaneous%20delivery%20in%20chickens&rft.jtitle=PloS%20one&rft.au=Alkie,%20Tamiru%20Negash&rft.date=2018-11-01&rft.volume=13&rft.issue=11&rft.spage=e0206324&rft.epage=e0206324&rft.pages=e0206324-e0206324&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0206324&rft_dat=%3Cproquest_plos_%3E2129536038%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2127945489&rft_id=info:pmid/30383798&rft_doaj_id=oai_doaj_org_article_cc4f341e8838430186f4ad50746f70a3&rfr_iscdi=true |