Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1
Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infections, such as pneumonia and bacteremia. Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection. In this study, we determined whether sulfated vizantin affects P. ae...
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| creator | Hayashi, Naoki Furue, Yui Kai, Daichi Yamada, Noriteru Yamamoto, Hirofumi Nakano, Takashi Oda, Masataka |
| description | Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infections, such as pneumonia and bacteremia. Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection. In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity. We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity. To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ. These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa. |
| doi_str_mv | 10.1371/journal.pone.0206696 |
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Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection. In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity. We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity. To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ. These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0206696</identifier><identifier>PMID: 30383847</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibiotics ; Antimicrobial activity ; Bacteremia ; Biology and Life Sciences ; Chemotaxis ; CheW protein ; CheZ protein ; Composition ; Cystic fibrosis ; Disease control ; Flagella ; Gene expression ; Genes ; Infections ; Medicine and Health Sciences ; Motility ; Mucin ; Opportunist infection ; Pharmaceuticals ; Pneumonia ; Proteins ; Pseudomonas aeruginosa ; Urogenital system ; Virulence ; Virulence factors</subject><ispartof>PloS one, 2018-11, Vol.13 (11), p.e0206696-e0206696</ispartof><rights>2018 Hayashi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Hayashi et al 2018 Hayashi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-b99c501652b5f864975a719d06ad01a5d0db6ae178a329c74cac77ca8bcf9e623</citedby><cites>FETCH-LOGICAL-c526t-b99c501652b5f864975a719d06ad01a5d0db6ae178a329c74cac77ca8bcf9e623</cites><orcidid>0000-0001-7435-8361</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211736/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211736/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30383847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hayashi, Naoki</creatorcontrib><creatorcontrib>Furue, Yui</creatorcontrib><creatorcontrib>Kai, Daichi</creatorcontrib><creatorcontrib>Yamada, Noriteru</creatorcontrib><creatorcontrib>Yamamoto, Hirofumi</creatorcontrib><creatorcontrib>Nakano, Takashi</creatorcontrib><creatorcontrib>Oda, Masataka</creatorcontrib><title>Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infections, such as pneumonia and bacteremia. Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection. In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity. We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity. To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ. These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.</description><subject>Antibiotics</subject><subject>Antimicrobial activity</subject><subject>Bacteremia</subject><subject>Biology and Life Sciences</subject><subject>Chemotaxis</subject><subject>CheW protein</subject><subject>CheZ protein</subject><subject>Composition</subject><subject>Cystic fibrosis</subject><subject>Disease control</subject><subject>Flagella</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Infections</subject><subject>Medicine and Health Sciences</subject><subject>Motility</subject><subject>Mucin</subject><subject>Opportunist infection</subject><subject>Pharmaceuticals</subject><subject>Pneumonia</subject><subject>Proteins</subject><subject>Pseudomonas aeruginosa</subject><subject>Urogenital system</subject><subject>Virulence</subject><subject>Virulence 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Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection. In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity. We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity. To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ. These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30383847</pmid><doi>10.1371/journal.pone.0206696</doi><orcidid>https://orcid.org/0000-0001-7435-8361</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antibiotics Antimicrobial activity Bacteremia Biology and Life Sciences Chemotaxis CheW protein CheZ protein Composition Cystic fibrosis Disease control Flagella Gene expression Genes Infections Medicine and Health Sciences Motility Mucin Opportunist infection Pharmaceuticals Pneumonia Proteins Pseudomonas aeruginosa Urogenital system Virulence Virulence factors |
| title | Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1 |
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