Development and biological evaluation of Ti6Al7Nb scaffold implants coated with gentamycin-saturated bacterial cellulose biomaterial
Herein we present an innovative method of coating the surface of Titanium-Aluminium-Niobium bone scaffold implants with bacterial cellulose (BC) polymer saturated with antibiotic. Customized Ti6Al7Nb scaffolds manufactured using Selective Laser Melting were immersed in a suspension of Komagataeibact...
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| creator | Dydak, Karolina Junka, Adam Szymczyk, Patrycja Chodaczek, Grzegorz Toporkiewicz, Monika Fijałkowski, Karol Dudek, Bartłomiej Bartoszewicz, Marzenna |
| description | Herein we present an innovative method of coating the surface of Titanium-Aluminium-Niobium bone scaffold implants with bacterial cellulose (BC) polymer saturated with antibiotic. Customized Ti6Al7Nb scaffolds manufactured using Selective Laser Melting were immersed in a suspension of Komagataeibacter xylinus bacteria which displays an ability to produce a 3-dimensional structure of bio-cellulose polymer. The process of complete implant coating with BC took on average 7 days. Subsequently, the BC matrix was cleansed by means of alkaline lysis and saturated with gentamycin. Scanning electron microscopy revealed that BC adheres and penetrates into the implant scaffold structure. The viability and development of the cellular layer on BC micro-structure were visualized by means of confocal microscopy. The BC-coated implants displayed a significantly lower cytotoxicity against osteoblast and fibroblast cell cultures in vitro in comparison to non-coated implants. It was also noted that gentamycin released from BC-coated implants inhibited the growth of Staphylococcus aureus cultures in vitro, confirming the suitability of such implant modification for preventing hostile microbial colonization. As demonstrated using digital microscopy, the procedure used for implant coating and BC chemical cleansing did not flaw the biomaterial structure. The results presented herein are of high translational value with regard to future use of customized, BC-coated and antibiotic-saturated implants designed for use in orthopedic applications to speed up recovery and to reduce the risk of musculoskeletal infections. |
| doi_str_mv | 10.1371/journal.pone.0205205 |
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Customized Ti6Al7Nb scaffolds manufactured using Selective Laser Melting were immersed in a suspension of Komagataeibacter xylinus bacteria which displays an ability to produce a 3-dimensional structure of bio-cellulose polymer. The process of complete implant coating with BC took on average 7 days. Subsequently, the BC matrix was cleansed by means of alkaline lysis and saturated with gentamycin. Scanning electron microscopy revealed that BC adheres and penetrates into the implant scaffold structure. The viability and development of the cellular layer on BC micro-structure were visualized by means of confocal microscopy. The BC-coated implants displayed a significantly lower cytotoxicity against osteoblast and fibroblast cell cultures in vitro in comparison to non-coated implants. It was also noted that gentamycin released from BC-coated implants inhibited the growth of Staphylococcus aureus cultures in vitro, confirming the suitability of such implant modification for preventing hostile microbial colonization. As demonstrated using digital microscopy, the procedure used for implant coating and BC chemical cleansing did not flaw the biomaterial structure. The results presented herein are of high translational value with regard to future use of customized, BC-coated and antibiotic-saturated implants designed for use in orthopedic applications to speed up recovery and to reduce the risk of musculoskeletal infections.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0205205</identifier><identifier>PMID: 30356274</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aluminum ; Antibiotics ; Antimicrobial agents ; Bacteria ; Biocompatibility ; Biofilms ; Biology and Life Sciences ; Biomaterials ; Biomedical materials ; Bone implants ; Bone surgery ; Cell adhesion & migration ; Cellular structure ; Cellulose ; Cleaning ; Coating ; Coatings ; Colonization ; Confocal microscopy ; Corrosion ; Cytotoxicity ; Dosage and administration ; Drug delivery systems ; Drugs ; Electron microscopy ; Engineering and Technology ; Fractures ; Gentamicin ; Gentamicins ; Health risks ; Joint surgery ; Laboratories ; Laser beam melting ; Lysis ; Manufacturing ; Medicine and Health Sciences ; Microorganisms ; Microscopy ; Niobium ; Organic chemistry ; Orthopedic implants ; Parasitology ; Pharmaceuticals ; Physical Sciences ; Polymers ; Risk reduction ; Scaffolds ; Scanning electron microscopy ; Staphylococcus infections ; Surgical implants ; Titanium ; Titanium base alloys ; Toxicity ; Transplants & implants ; Viability</subject><ispartof>PloS one, 2018-10, Vol.13 (10), p.e0205205-e0205205</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Dydak et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Dydak et al 2018 Dydak et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-16cd8cdb5cf69102de0cb0567e80b3b2fa6667f46ca749bfe1539afc15cf91593</citedby><cites>FETCH-LOGICAL-c692t-16cd8cdb5cf69102de0cb0567e80b3b2fa6667f46ca749bfe1539afc15cf91593</cites><orcidid>0000-0002-4915-6298</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200220/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200220/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30356274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mukherjee, Amitava</contributor><creatorcontrib>Dydak, Karolina</creatorcontrib><creatorcontrib>Junka, Adam</creatorcontrib><creatorcontrib>Szymczyk, Patrycja</creatorcontrib><creatorcontrib>Chodaczek, Grzegorz</creatorcontrib><creatorcontrib>Toporkiewicz, Monika</creatorcontrib><creatorcontrib>Fijałkowski, Karol</creatorcontrib><creatorcontrib>Dudek, Bartłomiej</creatorcontrib><creatorcontrib>Bartoszewicz, Marzenna</creatorcontrib><title>Development and biological evaluation of Ti6Al7Nb scaffold implants coated with gentamycin-saturated bacterial cellulose biomaterial</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Herein we present an innovative method of coating the surface of Titanium-Aluminium-Niobium bone scaffold implants with bacterial cellulose (BC) polymer saturated with antibiotic. Customized Ti6Al7Nb scaffolds manufactured using Selective Laser Melting were immersed in a suspension of Komagataeibacter xylinus bacteria which displays an ability to produce a 3-dimensional structure of bio-cellulose polymer. The process of complete implant coating with BC took on average 7 days. Subsequently, the BC matrix was cleansed by means of alkaline lysis and saturated with gentamycin. Scanning electron microscopy revealed that BC adheres and penetrates into the implant scaffold structure. The viability and development of the cellular layer on BC micro-structure were visualized by means of confocal microscopy. The BC-coated implants displayed a significantly lower cytotoxicity against osteoblast and fibroblast cell cultures in vitro in comparison to non-coated implants. It was also noted that gentamycin released from BC-coated implants inhibited the growth of Staphylococcus aureus cultures in vitro, confirming the suitability of such implant modification for preventing hostile microbial colonization. As demonstrated using digital microscopy, the procedure used for implant coating and BC chemical cleansing did not flaw the biomaterial structure. The results presented herein are of high translational value with regard to future use of customized, BC-coated and antibiotic-saturated implants designed for use in orthopedic applications to speed up recovery and to reduce the risk of musculoskeletal infections.</description><subject>Aluminum</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Biocompatibility</subject><subject>Biofilms</subject><subject>Biology and Life Sciences</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Bone implants</subject><subject>Bone surgery</subject><subject>Cell adhesion & migration</subject><subject>Cellular structure</subject><subject>Cellulose</subject><subject>Cleaning</subject><subject>Coating</subject><subject>Coatings</subject><subject>Colonization</subject><subject>Confocal microscopy</subject><subject>Corrosion</subject><subject>Cytotoxicity</subject><subject>Dosage and 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and biological evaluation of Ti6Al7Nb scaffold implants coated with gentamycin-saturated bacterial cellulose biomaterial</title><author>Dydak, Karolina ; Junka, Adam ; Szymczyk, Patrycja ; Chodaczek, Grzegorz ; Toporkiewicz, Monika ; Fijałkowski, Karol ; Dudek, Bartłomiej ; Bartoszewicz, Marzenna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-16cd8cdb5cf69102de0cb0567e80b3b2fa6667f46ca749bfe1539afc15cf91593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Aluminum</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Biocompatibility</topic><topic>Biofilms</topic><topic>Biology and Life Sciences</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Bone implants</topic><topic>Bone surgery</topic><topic>Cell adhesion & migration</topic><topic>Cellular 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One</addtitle><date>2018-10-24</date><risdate>2018</risdate><volume>13</volume><issue>10</issue><spage>e0205205</spage><epage>e0205205</epage><pages>e0205205-e0205205</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Herein we present an innovative method of coating the surface of Titanium-Aluminium-Niobium bone scaffold implants with bacterial cellulose (BC) polymer saturated with antibiotic. Customized Ti6Al7Nb scaffolds manufactured using Selective Laser Melting were immersed in a suspension of Komagataeibacter xylinus bacteria which displays an ability to produce a 3-dimensional structure of bio-cellulose polymer. The process of complete implant coating with BC took on average 7 days. Subsequently, the BC matrix was cleansed by means of alkaline lysis and saturated with gentamycin. Scanning electron microscopy revealed that BC adheres and penetrates into the implant scaffold structure. The viability and development of the cellular layer on BC micro-structure were visualized by means of confocal microscopy. The BC-coated implants displayed a significantly lower cytotoxicity against osteoblast and fibroblast cell cultures in vitro in comparison to non-coated implants. It was also noted that gentamycin released from BC-coated implants inhibited the growth of Staphylococcus aureus cultures in vitro, confirming the suitability of such implant modification for preventing hostile microbial colonization. As demonstrated using digital microscopy, the procedure used for implant coating and BC chemical cleansing did not flaw the biomaterial structure. The results presented herein are of high translational value with regard to future use of customized, BC-coated and antibiotic-saturated implants designed for use in orthopedic applications to speed up recovery and to reduce the risk of musculoskeletal infections.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30356274</pmid><doi>10.1371/journal.pone.0205205</doi><tpages>e0205205</tpages><orcidid>https://orcid.org/0000-0002-4915-6298</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-10, Vol.13 (10), p.e0205205-e0205205 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2124861785 |
| source | Public Library of Science (PLoS) Journals Open Access; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aluminum Antibiotics Antimicrobial agents Bacteria Biocompatibility Biofilms Biology and Life Sciences Biomaterials Biomedical materials Bone implants Bone surgery Cell adhesion & migration Cellular structure Cellulose Cleaning Coating Coatings Colonization Confocal microscopy Corrosion Cytotoxicity Dosage and administration Drug delivery systems Drugs Electron microscopy Engineering and Technology Fractures Gentamicin Gentamicins Health risks Joint surgery Laboratories Laser beam melting Lysis Manufacturing Medicine and Health Sciences Microorganisms Microscopy Niobium Organic chemistry Orthopedic implants Parasitology Pharmaceuticals Physical Sciences Polymers Risk reduction Scaffolds Scanning electron microscopy Staphylococcus infections Surgical implants Titanium Titanium base alloys Toxicity Transplants & implants Viability |
| title | Development and biological evaluation of Ti6Al7Nb scaffold implants coated with gentamycin-saturated bacterial cellulose biomaterial |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-11-30T04%3A47%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20and%20biological%20evaluation%20of%20Ti6Al7Nb%20scaffold%20implants%20coated%20with%20gentamycin-saturated%20bacterial%20cellulose%20biomaterial&rft.jtitle=PloS%20one&rft.au=Dydak,%20Karolina&rft.date=2018-10-24&rft.volume=13&rft.issue=10&rft.spage=e0205205&rft.epage=e0205205&rft.pages=e0205205-e0205205&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0205205&rft_dat=%3Cgale_plos_%3EA559617249%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2124861785&rft_id=info:pmid/30356274&rft_galeid=A559617249&rft_doaj_id=oai_doaj_org_article_a9acad4aa057496f8cda50405dac77e1&rfr_iscdi=true |