A wheat cytochrome P450 enhances both resistance to deoxynivalenol and grain yield
The mycotoxin deoxynivalenol (DON) serves as a plant disease virulence factor for the fungi Fusarium graminearum and F. culmorum during the development of Fusarium head blight (FHB) disease on wheat. A wheat cytochrome P450 gene from the subfamily CYP72A, TaCYP72A, was cloned from wheat cultivar CM8...
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| creator | Gunupuru, Lokanadha R Arunachalam, Chanemougasoundharam Malla, Keshav B Kahla, Amal Perochon, Alexandre Jia, Jianguang Thapa, Ganesh Doohan, Fiona M |
| description | The mycotoxin deoxynivalenol (DON) serves as a plant disease virulence factor for the fungi Fusarium graminearum and F. culmorum during the development of Fusarium head blight (FHB) disease on wheat. A wheat cytochrome P450 gene from the subfamily CYP72A, TaCYP72A, was cloned from wheat cultivar CM82036. TaCYP72A was located on chromosome 3A with homeologs present on 3B and 3D of the wheat genome. Using gene expression studies, we showed that TaCYP72A variants were activated in wheat spikelets as an early response to F. graminearum, and this activation was in response to the mycotoxic Fusarium virulence factor deoxynivalenol (DON). Virus induced gene silencing (VIGS) studies in wheat heads revealed that this gene family contributes to DON resistance. VIGS resulted in more DON-induced discoloration of spikelets, as compared to mock VIGS treatment. In addition to positively affecting DON resistance, TaCYP72A also had a positive effect on grain number. VIGS of TaCYP72A genes reduced grain number by more than 59%. Thus, we provide evidence that TaCYP72A contributes to host resistance to DON and conclude that this gene family warrants further assessment as positive contributors to both biotic stress resistance and grain development in wheat. |
| doi_str_mv | 10.1371/journal.pone.0204992 |
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A wheat cytochrome P450 gene from the subfamily CYP72A, TaCYP72A, was cloned from wheat cultivar CM82036. TaCYP72A was located on chromosome 3A with homeologs present on 3B and 3D of the wheat genome. Using gene expression studies, we showed that TaCYP72A variants were activated in wheat spikelets as an early response to F. graminearum, and this activation was in response to the mycotoxic Fusarium virulence factor deoxynivalenol (DON). Virus induced gene silencing (VIGS) studies in wheat heads revealed that this gene family contributes to DON resistance. VIGS resulted in more DON-induced discoloration of spikelets, as compared to mock VIGS treatment. In addition to positively affecting DON resistance, TaCYP72A also had a positive effect on grain number. VIGS of TaCYP72A genes reduced grain number by more than 59%. Thus, we provide evidence that TaCYP72A contributes to host resistance to DON and conclude that this gene family warrants further assessment as positive contributors to both biotic stress resistance and grain development in wheat.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0204992</identifier><identifier>PMID: 30312356</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Barley ; Biology ; Biology and Life Sciences ; Blight ; Computer and Information Sciences ; Crop yield ; Crop yields ; Cultivars ; Cytochrome ; Cytochrome P-450 ; Cytochrome P-450 Enzyme System - classification ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; Cytochrome P450 ; Cytochromes P450 ; Deoxynivalenol ; Deoxyribonucleic acid ; Discoloration ; Disease ; Disease Resistance - drug effects ; DNA ; Earth Sciences ; Edible Grain - physiology ; Enzymes ; Fungi ; Fusarium ; Fusarium - isolation & purification ; Fusarium - metabolism ; Fusarium culmorum ; Fusarium graminearum ; Fusarium head blight ; Gene expression ; Gene Expression Regulation, Plant - drug effects ; Gene Silencing ; Genetic aspects ; Genetic Vectors - genetics ; Genetic Vectors - metabolism ; Genomes ; Genomics ; Grain ; Host-Pathogen Interactions - drug effects ; Medicine and Health Sciences ; Mutagenesis ; Mycotoxins ; Phylogeny ; Physiological aspects ; Plant diseases ; Plant Diseases - microbiology ; Plant Diseases - prevention & control ; Plant Proteins - classification ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Viruses - genetics ; Research and analysis methods ; Seeds ; Trichothecenes ; Trichothecenes - pharmacology ; Triticum - growth & development ; Triticum - metabolism ; Virulence ; Virulence Factors ; Viruses ; Wheat</subject><ispartof>PloS one, 2018-10, Vol.13 (10), p.e0204992</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Gunupuru et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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physiology</subject><subject>Enzymes</subject><subject>Fungi</subject><subject>Fusarium</subject><subject>Fusarium - isolation & purification</subject><subject>Fusarium - metabolism</subject><subject>Fusarium culmorum</subject><subject>Fusarium graminearum</subject><subject>Fusarium head blight</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Gene Silencing</subject><subject>Genetic aspects</subject><subject>Genetic Vectors - genetics</subject><subject>Genetic Vectors - metabolism</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Grain</subject><subject>Host-Pathogen Interactions - drug effects</subject><subject>Medicine and Health Sciences</subject><subject>Mutagenesis</subject><subject>Mycotoxins</subject><subject>Phylogeny</subject><subject>Physiological aspects</subject><subject>Plant diseases</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Diseases - prevention & control</subject><subject>Plant Proteins - classification</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Viruses - genetics</subject><subject>Research and analysis methods</subject><subject>Seeds</subject><subject>Trichothecenes</subject><subject>Trichothecenes - pharmacology</subject><subject>Triticum - growth & development</subject><subject>Triticum - metabolism</subject><subject>Virulence</subject><subject>Virulence Factors</subject><subject>Viruses</subject><subject>Wheat</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkluLGyEcxYfS0r2036C0QmGhD0m9jKPzUghLL4GFLdvLqzjqzBgmmlWz3Xz7mmZ2yUALxQfl-Psf5XCK4hWCc0QYer_y2-DkMN94Z-YQw7Ku8ZPiFNUEzyoMydOj80lxFuMKQkp4VT0vTggkCBNanRY3C_CrNzIBtUte9cGvDfhaUgiM66VTJoLGpx4EE21MewEkD7Tx9ztn7-RgnB-AdBp0QVoHdtYM-kXxrJVDNC_H_bz48enj98svs6vrz8vLxdVMMcrTDBEtZSUVpUQRhjVFLeYGQ9RwjlhVYl6jVsoa1yXnTGe1xKqUpVIUKUkbcl68OfhuBh_FGEcUGCHOOMcMZWJ5ILSXK7EJdi3DTnhpxR_Bh07IkKwajGCYcaxhLZGipa5QXTOuqKGIwlI3SGevD-Nr22ZttDIuBTlMTKc3zvai83eiQpwyvP_M29Eg-NutiekfXx6pLocrrGt9NlNrG5VYUFpXiHG6p-Z_ofLSZm1VLkRrsz4ZeDcZyEwy96mT2xjF8tvN_7PXP6fsxRGbizSkPvphm6x3cQqWB1AFH2Mw7WNyCIp9nx_SEPs-i7HPeez1ceqPQw8FJr8BneLulg</recordid><startdate>20181012</startdate><enddate>20181012</enddate><creator>Gunupuru, Lokanadha R</creator><creator>Arunachalam, Chanemougasoundharam</creator><creator>Malla, Keshav B</creator><creator>Kahla, Amal</creator><creator>Perochon, Alexandre</creator><creator>Jia, Jianguang</creator><creator>Thapa, Ganesh</creator><creator>Doohan, Fiona M</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1953-6070</orcidid></search><sort><creationdate>20181012</creationdate><title>A wheat cytochrome P450 enhances both resistance to deoxynivalenol and grain yield</title><author>Gunupuru, Lokanadha R ; Arunachalam, Chanemougasoundharam ; Malla, Keshav B ; Kahla, Amal ; Perochon, Alexandre ; Jia, Jianguang ; Thapa, Ganesh ; Doohan, Fiona M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-13daa6ac553c372d51f28e201b8817642891faa9294887db8842c4a4cc51ca5b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Barley</topic><topic>Biology</topic><topic>Biology and Life Sciences</topic><topic>Blight</topic><topic>Computer and Information Sciences</topic><topic>Crop yield</topic><topic>Crop yields</topic><topic>Cultivars</topic><topic>Cytochrome</topic><topic>Cytochrome P-450</topic><topic>Cytochrome P-450 Enzyme System - 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A wheat cytochrome P450 gene from the subfamily CYP72A, TaCYP72A, was cloned from wheat cultivar CM82036. TaCYP72A was located on chromosome 3A with homeologs present on 3B and 3D of the wheat genome. Using gene expression studies, we showed that TaCYP72A variants were activated in wheat spikelets as an early response to F. graminearum, and this activation was in response to the mycotoxic Fusarium virulence factor deoxynivalenol (DON). Virus induced gene silencing (VIGS) studies in wheat heads revealed that this gene family contributes to DON resistance. VIGS resulted in more DON-induced discoloration of spikelets, as compared to mock VIGS treatment. In addition to positively affecting DON resistance, TaCYP72A also had a positive effect on grain number. VIGS of TaCYP72A genes reduced grain number by more than 59%. Thus, we provide evidence that TaCYP72A contributes to host resistance to DON and conclude that this gene family warrants further assessment as positive contributors to both biotic stress resistance and grain development in wheat.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30312356</pmid><doi>10.1371/journal.pone.0204992</doi><tpages>e0204992</tpages><orcidid>https://orcid.org/0000-0002-1953-6070</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_2118788271 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Barley Biology Biology and Life Sciences Blight Computer and Information Sciences Crop yield Crop yields Cultivars Cytochrome Cytochrome P-450 Cytochrome P-450 Enzyme System - classification Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Cytochrome P450 Cytochromes P450 Deoxynivalenol Deoxyribonucleic acid Discoloration Disease Disease Resistance - drug effects DNA Earth Sciences Edible Grain - physiology Enzymes Fungi Fusarium Fusarium - isolation & purification Fusarium - metabolism Fusarium culmorum Fusarium graminearum Fusarium head blight Gene expression Gene Expression Regulation, Plant - drug effects Gene Silencing Genetic aspects Genetic Vectors - genetics Genetic Vectors - metabolism Genomes Genomics Grain Host-Pathogen Interactions - drug effects Medicine and Health Sciences Mutagenesis Mycotoxins Phylogeny Physiological aspects Plant diseases Plant Diseases - microbiology Plant Diseases - prevention & control Plant Proteins - classification Plant Proteins - genetics Plant Proteins - metabolism Plant Viruses - genetics Research and analysis methods Seeds Trichothecenes Trichothecenes - pharmacology Triticum - growth & development Triticum - metabolism Virulence Virulence Factors Viruses Wheat |
| title | A wheat cytochrome P450 enhances both resistance to deoxynivalenol and grain yield |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T04%3A05%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20wheat%20cytochrome%20P450%20enhances%20both%20resistance%20to%20deoxynivalenol%20and%20grain%20yield&rft.jtitle=PloS%20one&rft.au=Gunupuru,%20Lokanadha%20R&rft.date=2018-10-12&rft.volume=13&rft.issue=10&rft.spage=e0204992&rft.pages=e0204992-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0204992&rft_dat=%3Cgale_plos_%3EA559617851%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2118788271&rft_id=info:pmid/30312356&rft_galeid=A559617851&rft_doaj_id=oai_doaj_org_article_72782d09a1c54d619978c5e51504db1d&rfr_iscdi=true |