Atomic force microscopy reveals new biophysical markers for monitoring subcellular changes in oxidative injury: Neuroprotective effects of quercetin at the nanoscale
Oxidative stress has been recognised as an important pathological mechanism underlying the development of neurodegenerative diseases. The biomarkers for assessing the degree of oxidative stress have been attracting much interest because of their potential clinical relevance in understanding the cell...
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| description | Oxidative stress has been recognised as an important pathological mechanism underlying the development of neurodegenerative diseases. The biomarkers for assessing the degree of oxidative stress have been attracting much interest because of their potential clinical relevance in understanding the cellular effects of free radicals and evaluation of the efficacy of drug treatment. Here, an interdisciplinary approach using atomic force microscopy (AFM) and cellular and biological molecular methods were used to investigate oxidative damage in P19 neurons and to reveal the underlying mechanism of protective action of quercetin. Biological methods demonstrated the oxidative damage of P19 neurons and showed that quercetin improved neuronal survival by preventing H2O2-induced p53 and Bcl-2 down-regulation and modulated Akt and ERK1/2 signalling pathways. For the first time, AFM was employed to evaluate morphologically (roughness, height, Feret dimension) and nanomechanical (elasticity) properties in H2O2-induced neuronal damage. The AFM analysis revealed that quercetin suppressed H2O2-provoked changes in cell membrane elasticity and morphological properties, thus confirming its neuroprotective activity. The obtained results indicate the potential of AFM-measured parameters as a biophysical markers of oxidative stress-induced neurodegeneration. In general, our study suggests that AFM can be used as a highly valuable tool in other biomedical applications aimed at screening and monitoring of drug-induced effects at cellular level. |
| doi_str_mv | 10.1371/journal.pone.0200119 |
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The biomarkers for assessing the degree of oxidative stress have been attracting much interest because of their potential clinical relevance in understanding the cellular effects of free radicals and evaluation of the efficacy of drug treatment. Here, an interdisciplinary approach using atomic force microscopy (AFM) and cellular and biological molecular methods were used to investigate oxidative damage in P19 neurons and to reveal the underlying mechanism of protective action of quercetin. Biological methods demonstrated the oxidative damage of P19 neurons and showed that quercetin improved neuronal survival by preventing H2O2-induced p53 and Bcl-2 down-regulation and modulated Akt and ERK1/2 signalling pathways. For the first time, AFM was employed to evaluate morphologically (roughness, height, Feret dimension) and nanomechanical (elasticity) properties in H2O2-induced neuronal damage. The AFM analysis revealed that quercetin suppressed H2O2-provoked changes in cell membrane elasticity and morphological properties, thus confirming its neuroprotective activity. The obtained results indicate the potential of AFM-measured parameters as a biophysical markers of oxidative stress-induced neurodegeneration. In general, our study suggests that AFM can be used as a highly valuable tool in other biomedical applications aimed at screening and monitoring of drug-induced effects at cellular level.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0200119</identifier><identifier>PMID: 30303965</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>AKT protein ; Alzheimer's disease ; Alzheimers disease ; Analysis ; Antioxidants ; Apoptosis ; Atomic force microscopy ; Bcl-2 protein ; Bioindicators ; Biological markers ; Biology and Life Sciences ; Biomarkers ; Biomedical materials ; Cell membranes ; Cell survival ; Damage ; Diagnosis ; Elasticity ; Extracellular signal-regulated kinase ; Free radicals ; Gene expression ; Hydrogen peroxide ; Kinases ; Membrane elasticity ; Microscopy ; Molecular biology ; Monitoring ; Morphology ; Neurodegeneration ; Neurodegenerative diseases ; Neurological diseases ; Neurons ; Neuroprotection ; Oxidative stress ; p53 Protein ; Physical chemistry ; Physiology ; Proteins ; Quercetin ; Research and Analysis Methods ; Signal transduction ; Topography ; Transcription factors</subject><ispartof>PloS one, 2018-10, Vol.13 (10), p.e0200119-e0200119</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Jazvinšćak Jembrek et al. 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The biomarkers for assessing the degree of oxidative stress have been attracting much interest because of their potential clinical relevance in understanding the cellular effects of free radicals and evaluation of the efficacy of drug treatment. Here, an interdisciplinary approach using atomic force microscopy (AFM) and cellular and biological molecular methods were used to investigate oxidative damage in P19 neurons and to reveal the underlying mechanism of protective action of quercetin. Biological methods demonstrated the oxidative damage of P19 neurons and showed that quercetin improved neuronal survival by preventing H2O2-induced p53 and Bcl-2 down-regulation and modulated Akt and ERK1/2 signalling pathways. For the first time, AFM was employed to evaluate morphologically (roughness, height, Feret dimension) and nanomechanical (elasticity) properties in H2O2-induced neuronal damage. The AFM analysis revealed that quercetin suppressed H2O2-provoked changes in cell membrane elasticity and morphological properties, thus confirming its neuroprotective activity. The obtained results indicate the potential of AFM-measured parameters as a biophysical markers of oxidative stress-induced neurodegeneration. In general, our study suggests that AFM can be used as a highly valuable tool in other biomedical applications aimed at screening and monitoring of drug-induced effects at cellular level.</description><subject>AKT protein</subject><subject>Alzheimer's disease</subject><subject>Alzheimers disease</subject><subject>Analysis</subject><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Atomic force microscopy</subject><subject>Bcl-2 protein</subject><subject>Bioindicators</subject><subject>Biological markers</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biomedical materials</subject><subject>Cell membranes</subject><subject>Cell survival</subject><subject>Damage</subject><subject>Diagnosis</subject><subject>Elasticity</subject><subject>Extracellular signal-regulated kinase</subject><subject>Free radicals</subject><subject>Gene expression</subject><subject>Hydrogen peroxide</subject><subject>Kinases</subject><subject>Membrane elasticity</subject><subject>Microscopy</subject><subject>Molecular biology</subject><subject>Monitoring</subject><subject>Morphology</subject><subject>Neurodegeneration</subject><subject>Neurodegenerative diseases</subject><subject>Neurological diseases</subject><subject>Neurons</subject><subject>Neuroprotection</subject><subject>Oxidative stress</subject><subject>p53 Protein</subject><subject>Physical chemistry</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Quercetin</subject><subject>Research and Analysis Methods</subject><subject>Signal transduction</subject><subject>Topography</subject><subject>Transcription 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force microscopy reveals new biophysical markers for monitoring subcellular changes in oxidative injury: Neuroprotective effects of quercetin at the nanoscale</title><author>Jazvinscak Jembrek, Maja ; Vlainic, Josipa ; Cadez, Vida ; Segota, Suzana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-bb8e4c35f1da7e31646012343227983005cb0daa0c67f3bae5475490d20b14923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>AKT protein</topic><topic>Alzheimer's disease</topic><topic>Alzheimers disease</topic><topic>Analysis</topic><topic>Antioxidants</topic><topic>Apoptosis</topic><topic>Atomic force microscopy</topic><topic>Bcl-2 protein</topic><topic>Bioindicators</topic><topic>Biological markers</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Biomedical materials</topic><topic>Cell membranes</topic><topic>Cell 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markers for monitoring subcellular changes in oxidative injury: Neuroprotective effects of quercetin at the nanoscale</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-10-10</date><risdate>2018</risdate><volume>13</volume><issue>10</issue><spage>e0200119</spage><epage>e0200119</epage><pages>e0200119-e0200119</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Oxidative stress has been recognised as an important pathological mechanism underlying the development of neurodegenerative diseases. 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The AFM analysis revealed that quercetin suppressed H2O2-provoked changes in cell membrane elasticity and morphological properties, thus confirming its neuroprotective activity. The obtained results indicate the potential of AFM-measured parameters as a biophysical markers of oxidative stress-induced neurodegeneration. In general, our study suggests that AFM can be used as a highly valuable tool in other biomedical applications aimed at screening and monitoring of drug-induced effects at cellular level.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30303965</pmid><doi>10.1371/journal.pone.0200119</doi><orcidid>https://orcid.org/0000-0001-6416-3835</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | AKT protein Alzheimer's disease Alzheimers disease Analysis Antioxidants Apoptosis Atomic force microscopy Bcl-2 protein Bioindicators Biological markers Biology and Life Sciences Biomarkers Biomedical materials Cell membranes Cell survival Damage Diagnosis Elasticity Extracellular signal-regulated kinase Free radicals Gene expression Hydrogen peroxide Kinases Membrane elasticity Microscopy Molecular biology Monitoring Morphology Neurodegeneration Neurodegenerative diseases Neurological diseases Neurons Neuroprotection Oxidative stress p53 Protein Physical chemistry Physiology Proteins Quercetin Research and Analysis Methods Signal transduction Topography Transcription factors |
| title | Atomic force microscopy reveals new biophysical markers for monitoring subcellular changes in oxidative injury: Neuroprotective effects of quercetin at the nanoscale |
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