Eprobe mediated RT-qPCR for the detection of leukemia-associated fusion genes

The detection and quantification of leukemia-associated fusion gene transcripts play important roles in the diagnosis and follow-up of leukemias. To establish a standardized method without interlaboratory discrepancies, we developed a novel one-step reverse transcription quantitative PCR (RT-qPCR) a...

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Veröffentlicht in:	PloS one 2018-10, Vol.13 (10), p.e0202429-e0202429
Hauptverfasser:	Tsuchiya, Koji, Tabe, Yoko, Ai, Tomohiko, Ohkawa, Takahiro, Usui, Kengo, Yuri, Maiko, Misawa, Shigeki, Morishita, Soji, Takaku, Tomoiku, Kakimoto, Atsushi, Yang, Haeun, Matsushita, Hiromichi, Hanami, Takeshi, Yamanaka, Yasunari, Okuzawa, Atsushi, Horii, Takashi, Hayashizaki, Yoshihide, Ohsaka, Akimichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Assaying Biology and Life Sciences Cancer Deoxyribonucleic acid DNA Feasibility studies Fluorescence Fusion protein Fusion proteins Gene expression Genes Genetic aspects Genetic testing Genomics Hematology Hospitals Hybridization Innovations Isoforms Laboratories Leukemia Life sciences Medical diagnosis Medicine and Health Sciences Melting Melting curve Minimal residual disease Oligonucleotides Physical Sciences Polymerase chain reaction Preventive medicine Quality control Research and Analysis Methods Reverse transcription Runx1 protein Stem cells Systems development
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