Characterization of the blastogenic response to LPS of bovine peripheral blood mononuclear cells

Mitogens are diverse compounds of plant and microbial origin, widely employed to test immunocompetence in animals. The blastogenic response of bovine Peripheral Blood Mononuclear Cells (PBMC) to lypopolysaccharides (LPS) has been investigated in our laboratories for a long time. In particular, a pos...

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Veröffentlicht in:	PloS one 2018-10, Vol.13 (10), p.e0204827
Hauptverfasser:	Amadori, Massimo, Soares-Filipe, Joel Fernando, Riva, Federica, Vitali, Andrea, Ruggeri, Jessica, Lacetera, Nicola
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal models Animals B-Lymphocytes - cytology B-Lymphocytes - metabolism Biology and Life Sciences Blood Blood cells Bromodeoxyuridine - adverse effects Cattle CD4 antigen CD4-Positive T-Lymphocytes - cytology CD4-Positive T-Lymphocytes - metabolism Cell cycle Cell Cycle - drug effects Cell proliferation Cell Proliferation - drug effects Concanavalin A Concanavalin A - pharmacology Correlation Dairy cattle Disease resistance Endotoxins Female Flow cytometry Gene expression Gene Expression Profiling - veterinary Gene Expression Regulation - drug effects Genes Immunocompetence Immunological tolerance Indoleamine-Pyrrole 2,3,-Dioxygenase - genetics Inflammation Kinases Laboratories Leukocytes (mononuclear) Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - drug effects Lipopolysaccharides Lipopolysaccharides - adverse effects Lipopolysaccharides - pharmacology Lymphocyte transformation Lymphocytes Lymphocytes B Lymphocytes T Magnetic separation Medicine and Health Sciences Microorganisms Mitogens Peripheral blood mononuclear cells Replication S phase Stimulation TLR4 protein Toll-Like Receptor 4 - genetics Toll-like receptors Tryptophan 2,3-dioxygenase Tumor Necrosis Factor-alpha - genetics Tumor necrosis factor-α
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