Centrifugation-induced release of ATP from red blood cells
Centrifugation is the primary preparation step for isolating red blood cells (RBCs) from whole blood, including for use in studies focused on transduction of adenosine triphosphate (ATP), an important vasodilatory signaling molecule. Despite the wide use of centrifugation, little work has focused on...
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| Veröffentlicht in: | PloS one 2018-09, Vol.13 (9), p.e0203270-e0203270 |
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| description | Centrifugation is the primary preparation step for isolating red blood cells (RBCs) from whole blood, including for use in studies focused on transduction of adenosine triphosphate (ATP), an important vasodilatory signaling molecule. Despite the wide use of centrifugation, little work has focused on how the centrifugation itself affects release of ATP from RBCs prior to subsequent experimentation. Here we report that both the centrifugation force and duration have a pronounced impact on the concentration of ATP present in the packed RBCs following centrifugation. Multiple subsequent centrifugations yield extracellular ATP concentrations comparable to the amount released during the initial centrifugation, suggesting this effect is cumulative. Pairwise measurements of hemoglobin and ATP suggest the presence of ATP is primarily due to an increase in centrifugation-induced hemolysis. These results indicate that common centrifugation parameters, within the ranges explored here, can release ATP in quantities comparable to the low end of the range of values measured in typical ATP transduction experiments, potentially complicating experimental interpretation of those results. |
| doi_str_mv | 10.1371/journal.pone.0203270 |
| format | Article |
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These results indicate that common centrifugation parameters, within the ranges explored here, can release ATP in quantities comparable to the low end of the range of values measured in typical ATP transduction experiments, potentially complicating experimental interpretation of those results.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0203270</identifier><identifier>PMID: 30183749</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenosine triphosphate ; Adenosine Triphosphate - metabolism ; ATP ; Biology and Life Sciences ; Biomechanical Phenomena ; Blood ; Blood cells ; Centrifugation ; Centrifugation - methods ; Chemical engineering ; Cystic fibrosis ; Erythrocytes ; Erythrocytes - metabolism ; Experimentation ; Experiments ; Health aspects ; Hemoglobin ; Hemoglobins ; Hemoglobins - metabolism ; Humans ; Laboratories ; Mechanical properties ; Medicine and Health Sciences ; Morphology ; Physical Sciences ; Physiology ; Rangefinding ; Red blood cells ; Research and Analysis Methods ; Researchers ; Signal transduction ; Time Factors</subject><ispartof>PloS one, 2018-09, Vol.13 (9), p.e0203270-e0203270</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Mancuso et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Despite the wide use of centrifugation, little work has focused on how the centrifugation itself affects release of ATP from RBCs prior to subsequent experimentation. Here we report that both the centrifugation force and duration have a pronounced impact on the concentration of ATP present in the packed RBCs following centrifugation. Multiple subsequent centrifugations yield extracellular ATP concentrations comparable to the amount released during the initial centrifugation, suggesting this effect is cumulative. Pairwise measurements of hemoglobin and ATP suggest the presence of ATP is primarily due to an increase in centrifugation-induced hemolysis. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mancuso, Jordan E</au><au>Jayaraman, Anjana</au><au>Ristenpart, William D</au><au>Connes, Philippe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Centrifugation-induced release of ATP from red blood cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-09-05</date><risdate>2018</risdate><volume>13</volume><issue>9</issue><spage>e0203270</spage><epage>e0203270</epage><pages>e0203270-e0203270</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Centrifugation is the primary preparation step for isolating red blood cells (RBCs) from whole blood, including for use in studies focused on transduction of adenosine triphosphate (ATP), an important vasodilatory signaling molecule. Despite the wide use of centrifugation, little work has focused on how the centrifugation itself affects release of ATP from RBCs prior to subsequent experimentation. Here we report that both the centrifugation force and duration have a pronounced impact on the concentration of ATP present in the packed RBCs following centrifugation. Multiple subsequent centrifugations yield extracellular ATP concentrations comparable to the amount released during the initial centrifugation, suggesting this effect is cumulative. Pairwise measurements of hemoglobin and ATP suggest the presence of ATP is primarily due to an increase in centrifugation-induced hemolysis. 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| subjects | Adenosine triphosphate Adenosine Triphosphate - metabolism ATP Biology and Life Sciences Biomechanical Phenomena Blood Blood cells Centrifugation Centrifugation - methods Chemical engineering Cystic fibrosis Erythrocytes Erythrocytes - metabolism Experimentation Experiments Health aspects Hemoglobin Hemoglobins Hemoglobins - metabolism Humans Laboratories Mechanical properties Medicine and Health Sciences Morphology Physical Sciences Physiology Rangefinding Red blood cells Research and Analysis Methods Researchers Signal transduction Time Factors |
| title | Centrifugation-induced release of ATP from red blood cells |
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