Preventive effects of bovine colostrum supplementation in TNBS-induced colitis in mice
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder for which the current medical therapy is not completely effective. Bovine colostrum (BC) is a biological fluid rich in bioactive molecules that may have beneficial effects on several gastrointestinal disorders. The objectives of thi...
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| creator | Filipescu, Iulia Elena Leonardi, Leonardo Menchetti, Laura Guelfi, Gabriella Traina, Giovanna Casagrande-Proietti, Patrizia Piro, Federica Quattrone, Alda Barbato, Olimpia Brecchia, Gabriele |
| description | Inflammatory bowel disease (IBD) is a chronic inflammatory disorder for which the current medical therapy is not completely effective. Bovine colostrum (BC) is a biological fluid rich in bioactive molecules that may have beneficial effects on several gastrointestinal disorders. The objectives of this study were to assess the preventive effects of BC supplementation in a mouse model of 2,4,6 trinitrobenzene sulfonic acid (TNBS)-induced colitis using a multidisciplinary approach. Specifically, the following parameters were evaluated: (i) disease activity index (DAI), (ii) histological score, (iii) expression levels of TLR4, anti- and pro-inflammatory cytokines, and (iv) count of some bacterial species of the intestinal microbiota. Mice received a daily suspension of BC (BC group, n = 12) or saline solution (control, CN group, n = 12) for 21 days before the intrarectal inoculation with 1% of TNBS solution. BC was well tolerated and did not induce any histological damage or clinical symptoms. After TNBS treatment, BC group showed a reduction of body weight (BW) loss (P |
| doi_str_mv | 10.1371/journal.pone.0202929 |
| format | Article |
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Bovine colostrum (BC) is a biological fluid rich in bioactive molecules that may have beneficial effects on several gastrointestinal disorders. The objectives of this study were to assess the preventive effects of BC supplementation in a mouse model of 2,4,6 trinitrobenzene sulfonic acid (TNBS)-induced colitis using a multidisciplinary approach. Specifically, the following parameters were evaluated: (i) disease activity index (DAI), (ii) histological score, (iii) expression levels of TLR4, anti- and pro-inflammatory cytokines, and (iv) count of some bacterial species of the intestinal microbiota. Mice received a daily suspension of BC (BC group, n = 12) or saline solution (control, CN group, n = 12) for 21 days before the intrarectal inoculation with 1% of TNBS solution. BC was well tolerated and did not induce any histological damage or clinical symptoms. After TNBS treatment, BC group showed a reduction of body weight (BW) loss (P<0.01) and histological score (P<0.05) compared to CN. Moreover, the expression levels of TLR4 (P<0.05), IL-1β (P<0.001), IL-8 (P<0.001), and IL-10 (P<0.001) were lower in mice administered with BC, while the concentrations of TNF-α did not show any differences between groups. Finally, the supplementation with BC resulted in a differential response to TNBS treatment in the bacterial count. In CN group, E. coli and Enterococci increased (P<0.001), while Anaerobes (P<0.01), Lactobacilli, and Bifidobacteria (P<0.001) reduced. Conversely, no significant changes in bacterial load were found after the inoculation of TNBS in BC pre-treated mice. This study confirms that TNBS-induced colitis model in mice is useful for studying the mechanisms involved in IBD pathogenesis and shows that pre-treatment with BC reduces the intestinal damages and clinical signs of the colitis. Molecular mechanisms and intestinal microflora could be involved in the protective effect of colostrum.]]></description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0202929</identifier><identifier>PMID: 30138385</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Anaerobes ; Animals ; Antigens ; Bacteria ; Bacterial Load ; Binding sites ; Biology and Life Sciences ; Body weight ; Care and treatment ; Cattle ; Colitis ; Colitis - chemically induced ; Colitis - prevention & control ; Colostrum ; Cytokines ; Cytokines - metabolism ; Damage tolerance ; Disease ; E coli ; Gastrointestinal diseases ; Gastrointestinal Microbiome ; Gene expression ; Genetic aspects ; Health aspects ; Homeostasis ; Immune system ; Immunology ; Inflammation ; Inflammatory bowel disease ; Inflammatory bowel diseases ; Inflammatory diseases ; Inoculation ; Interleukin 10 ; Interleukin 8 ; Intestinal microflora ; Intestine ; Lactobacilli ; Lymphocytes ; Male ; Medicine and Health Sciences ; Mice ; Microbiota ; Molecular modelling ; Pathogenesis ; Permeability ; Properties ; Protective Agents - therapeutic use ; Proteins ; Research and Analysis Methods ; Rodents ; Saline solutions ; Sulfonic acid ; Sulfonic acids ; Supplements ; TLR4 protein ; Toll-Like Receptor 4 - metabolism ; Toll-like receptors ; Trinitrobenzenesulfonic Acid ; Tumor necrosis factor-α ; Veterinary medicine ; Weight reduction</subject><ispartof>PloS one, 2018-08, Vol.13 (8), p.e0202929</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Filipescu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Filipescu et al 2018 Filipescu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-caae1b826d88682ed1118d0967f92b5f1c494f5a66f496526b3b7405b6c065cd3</citedby><cites>FETCH-LOGICAL-c692t-caae1b826d88682ed1118d0967f92b5f1c494f5a66f496526b3b7405b6c065cd3</cites><orcidid>0000-0001-8500-7970 ; 0000-0003-1147-2540</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107273/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107273/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30138385$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Filipescu, Iulia Elena</creatorcontrib><creatorcontrib>Leonardi, Leonardo</creatorcontrib><creatorcontrib>Menchetti, Laura</creatorcontrib><creatorcontrib>Guelfi, Gabriella</creatorcontrib><creatorcontrib>Traina, Giovanna</creatorcontrib><creatorcontrib>Casagrande-Proietti, Patrizia</creatorcontrib><creatorcontrib>Piro, Federica</creatorcontrib><creatorcontrib>Quattrone, Alda</creatorcontrib><creatorcontrib>Barbato, Olimpia</creatorcontrib><creatorcontrib>Brecchia, Gabriele</creatorcontrib><title>Preventive effects of bovine colostrum supplementation in TNBS-induced colitis in mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description><![CDATA[Inflammatory bowel disease (IBD) is a chronic inflammatory disorder for which the current medical therapy is not completely effective. Bovine colostrum (BC) is a biological fluid rich in bioactive molecules that may have beneficial effects on several gastrointestinal disorders. The objectives of this study were to assess the preventive effects of BC supplementation in a mouse model of 2,4,6 trinitrobenzene sulfonic acid (TNBS)-induced colitis using a multidisciplinary approach. Specifically, the following parameters were evaluated: (i) disease activity index (DAI), (ii) histological score, (iii) expression levels of TLR4, anti- and pro-inflammatory cytokines, and (iv) count of some bacterial species of the intestinal microbiota. Mice received a daily suspension of BC (BC group, n = 12) or saline solution (control, CN group, n = 12) for 21 days before the intrarectal inoculation with 1% of TNBS solution. BC was well tolerated and did not induce any histological damage or clinical symptoms. After TNBS treatment, BC group showed a reduction of body weight (BW) loss (P<0.01) and histological score (P<0.05) compared to CN. Moreover, the expression levels of TLR4 (P<0.05), IL-1β (P<0.001), IL-8 (P<0.001), and IL-10 (P<0.001) were lower in mice administered with BC, while the concentrations of TNF-α did not show any differences between groups. Finally, the supplementation with BC resulted in a differential response to TNBS treatment in the bacterial count. In CN group, E. coli and Enterococci increased (P<0.001), while Anaerobes (P<0.01), Lactobacilli, and Bifidobacteria (P<0.001) reduced. Conversely, no significant changes in bacterial load were found after the inoculation of TNBS in BC pre-treated mice. This study confirms that TNBS-induced colitis model in mice is useful for studying the mechanisms involved in IBD pathogenesis and shows that pre-treatment with BC reduces the intestinal damages and clinical signs of the colitis. Molecular mechanisms and intestinal microflora could be involved in the protective effect of colostrum.]]></description><subject>Anaerobes</subject><subject>Animals</subject><subject>Antigens</subject><subject>Bacteria</subject><subject>Bacterial Load</subject><subject>Binding sites</subject><subject>Biology and Life Sciences</subject><subject>Body weight</subject><subject>Care and treatment</subject><subject>Cattle</subject><subject>Colitis</subject><subject>Colitis - chemically induced</subject><subject>Colitis - prevention & control</subject><subject>Colostrum</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Damage tolerance</subject><subject>Disease</subject><subject>E coli</subject><subject>Gastrointestinal diseases</subject><subject>Gastrointestinal Microbiome</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Homeostasis</subject><subject>Immune system</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Inflammatory bowel disease</subject><subject>Inflammatory bowel diseases</subject><subject>Inflammatory diseases</subject><subject>Inoculation</subject><subject>Interleukin 10</subject><subject>Interleukin 8</subject><subject>Intestinal microflora</subject><subject>Intestine</subject><subject>Lactobacilli</subject><subject>Lymphocytes</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Microbiota</subject><subject>Molecular modelling</subject><subject>Pathogenesis</subject><subject>Permeability</subject><subject>Properties</subject><subject>Protective Agents - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Filipescu, Iulia Elena</au><au>Leonardi, Leonardo</au><au>Menchetti, Laura</au><au>Guelfi, Gabriella</au><au>Traina, Giovanna</au><au>Casagrande-Proietti, Patrizia</au><au>Piro, Federica</au><au>Quattrone, Alda</au><au>Barbato, Olimpia</au><au>Brecchia, Gabriele</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preventive effects of bovine colostrum supplementation in TNBS-induced colitis in mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-08-23</date><risdate>2018</risdate><volume>13</volume><issue>8</issue><spage>e0202929</spage><pages>e0202929-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract><![CDATA[Inflammatory bowel disease (IBD) is a chronic inflammatory disorder for which the current medical therapy is not completely effective. Bovine colostrum (BC) is a biological fluid rich in bioactive molecules that may have beneficial effects on several gastrointestinal disorders. The objectives of this study were to assess the preventive effects of BC supplementation in a mouse model of 2,4,6 trinitrobenzene sulfonic acid (TNBS)-induced colitis using a multidisciplinary approach. Specifically, the following parameters were evaluated: (i) disease activity index (DAI), (ii) histological score, (iii) expression levels of TLR4, anti- and pro-inflammatory cytokines, and (iv) count of some bacterial species of the intestinal microbiota. Mice received a daily suspension of BC (BC group, n = 12) or saline solution (control, CN group, n = 12) for 21 days before the intrarectal inoculation with 1% of TNBS solution. BC was well tolerated and did not induce any histological damage or clinical symptoms. After TNBS treatment, BC group showed a reduction of body weight (BW) loss (P<0.01) and histological score (P<0.05) compared to CN. Moreover, the expression levels of TLR4 (P<0.05), IL-1β (P<0.001), IL-8 (P<0.001), and IL-10 (P<0.001) were lower in mice administered with BC, while the concentrations of TNF-α did not show any differences between groups. Finally, the supplementation with BC resulted in a differential response to TNBS treatment in the bacterial count. In CN group, E. coli and Enterococci increased (P<0.001), while Anaerobes (P<0.01), Lactobacilli, and Bifidobacteria (P<0.001) reduced. Conversely, no significant changes in bacterial load were found after the inoculation of TNBS in BC pre-treated mice. This study confirms that TNBS-induced colitis model in mice is useful for studying the mechanisms involved in IBD pathogenesis and shows that pre-treatment with BC reduces the intestinal damages and clinical signs of the colitis. Molecular mechanisms and intestinal microflora could be involved in the protective effect of colostrum.]]></abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30138385</pmid><doi>10.1371/journal.pone.0202929</doi><tpages>e0202929</tpages><orcidid>https://orcid.org/0000-0001-8500-7970</orcidid><orcidid>https://orcid.org/0000-0003-1147-2540</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-08, Vol.13 (8), p.e0202929 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2092586606 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Anaerobes Animals Antigens Bacteria Bacterial Load Binding sites Biology and Life Sciences Body weight Care and treatment Cattle Colitis Colitis - chemically induced Colitis - prevention & control Colostrum Cytokines Cytokines - metabolism Damage tolerance Disease E coli Gastrointestinal diseases Gastrointestinal Microbiome Gene expression Genetic aspects Health aspects Homeostasis Immune system Immunology Inflammation Inflammatory bowel disease Inflammatory bowel diseases Inflammatory diseases Inoculation Interleukin 10 Interleukin 8 Intestinal microflora Intestine Lactobacilli Lymphocytes Male Medicine and Health Sciences Mice Microbiota Molecular modelling Pathogenesis Permeability Properties Protective Agents - therapeutic use Proteins Research and Analysis Methods Rodents Saline solutions Sulfonic acid Sulfonic acids Supplements TLR4 protein Toll-Like Receptor 4 - metabolism Toll-like receptors Trinitrobenzenesulfonic Acid Tumor necrosis factor-α Veterinary medicine Weight reduction |
| title | Preventive effects of bovine colostrum supplementation in TNBS-induced colitis in mice |
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