Self-sampling coupled to the detection of HPV 16 and 18 E6 protein: A promising option for detection of cervical malignancies in remote areas
To evaluate both the performance and acceptability of a method coupling self-sampling with detection of cervical malignancy via elevated HPV 16 and 18 E6 oncoproteins (OncoE6™ Cervical Test) in remote areas in Brazil. Women living in rural villages in proximity to Coari city, Amazonas, Brazil were i...
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| creator | Torres, Kátia Luz Mariño, Josiane Montanho Pires Rocha, Danielle Albuquerque de Mello, Mônica Bandeira de Melo Farah, Heydy Halanna Reis, Renato Dos Santos Alves, Valquíria do Carmo Rodrigues Gomes, Edson Martins, Toni Ricardo Soares, Ana Carolina de Oliveira, Cristina Mendes Levi, José Eduardo |
| description | To evaluate both the performance and acceptability of a method coupling self-sampling with detection of cervical malignancy via elevated HPV 16 and 18 E6 oncoproteins (OncoE6™ Cervical Test) in remote areas in Brazil.
Women living in rural villages in proximity to Coari city, Amazonas, Brazil were invited to participate in a cervical cancer screening study. 412 subjects were enrolled; there were no refusals. In addition to E6 protein detection, DNA was extracted from the brushes and evaluated for HPV genotypes by PCR (PGMY09/11), followed by typing by the Papillocheck™ if positive. Subjects who were found to be positive for OncoE6 or HPV-DNA were referred for colposcopy.
For 110 subjects (27%) this was the first cervical cancer exam. Overall the HPV-DNA prevalence was 19.1% (n = 79); 1.4% (n = 6) were positive by the OncoE6 Test. Fifty-six women attended the invitation for colposcopy where nine had an abnormal cervix and were subsequently biopsied. Histopathological analysis revealed 2 CIN3, 2 carcinomas and 5 CIN1. OncoE6 called two out of the three HPV 16 or 18 associated CIN3+ lesions.
The findings suggest that self-administered sample collection in combination with OncoE6 Test is feasible in this population. This could enable expanded screening coverage while ensuring a high specificity which is imperative given the remote geographic location, since women bearing abnormal test results would necessitate travel and logistical burden to access colposcopy and treatment. |
| doi_str_mv | 10.1371/journal.pone.0201262 |
| format | Article |
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Women living in rural villages in proximity to Coari city, Amazonas, Brazil were invited to participate in a cervical cancer screening study. 412 subjects were enrolled; there were no refusals. In addition to E6 protein detection, DNA was extracted from the brushes and evaluated for HPV genotypes by PCR (PGMY09/11), followed by typing by the Papillocheck™ if positive. Subjects who were found to be positive for OncoE6 or HPV-DNA were referred for colposcopy.
For 110 subjects (27%) this was the first cervical cancer exam. Overall the HPV-DNA prevalence was 19.1% (n = 79); 1.4% (n = 6) were positive by the OncoE6 Test. Fifty-six women attended the invitation for colposcopy where nine had an abnormal cervix and were subsequently biopsied. Histopathological analysis revealed 2 CIN3, 2 carcinomas and 5 CIN1. OncoE6 called two out of the three HPV 16 or 18 associated CIN3+ lesions.
The findings suggest that self-administered sample collection in combination with OncoE6 Test is feasible in this population. This could enable expanded screening coverage while ensuring a high specificity which is imperative given the remote geographic location, since women bearing abnormal test results would necessitate travel and logistical burden to access colposcopy and treatment.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0201262</identifier><identifier>PMID: 30036381</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Biology and life sciences ; Cancer ; Cancer screening ; Carcinoma ; Cellular biology ; Cervical cancer ; Colposcopy ; Deoxyribonucleic acid ; Diagnosis ; DNA ; E6 protein ; Genotypes ; Human papillomavirus ; Lesions ; Malignancy ; Medical screening ; Medicine and Health Sciences ; Papillomavirus infections ; People and places ; Proteins ; Public health ; Remote regions ; Research and Analysis Methods ; Risk factors ; Rural areas ; Sampling ; Systematic review ; Womens health</subject><ispartof>PloS one, 2018-07, Vol.13 (7), p.e0201262-e0201262</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Torres et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Torres et al 2018 Torres et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c585t-a7fa40500aba09fb9986fd1a22fa6aad63b63e76c48eb700708b350f33d8bc093</citedby><orcidid>0000-0002-3557-2717</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056043/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056043/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30036381$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Torres, Kátia Luz</creatorcontrib><creatorcontrib>Mariño, Josiane Montanho</creatorcontrib><creatorcontrib>Pires Rocha, Danielle Albuquerque</creatorcontrib><creatorcontrib>de Mello, Mônica Bandeira</creatorcontrib><creatorcontrib>de Melo Farah, Heydy Halanna</creatorcontrib><creatorcontrib>Reis, Renato Dos Santos</creatorcontrib><creatorcontrib>Alves, Valquíria do Carmo Rodrigues</creatorcontrib><creatorcontrib>Gomes, Edson</creatorcontrib><creatorcontrib>Martins, Toni Ricardo</creatorcontrib><creatorcontrib>Soares, Ana Carolina</creatorcontrib><creatorcontrib>de Oliveira, Cristina Mendes</creatorcontrib><creatorcontrib>Levi, José Eduardo</creatorcontrib><title>Self-sampling coupled to the detection of HPV 16 and 18 E6 protein: A promising option for detection of cervical malignancies in remote areas</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>To evaluate both the performance and acceptability of a method coupling self-sampling with detection of cervical malignancy via elevated HPV 16 and 18 E6 oncoproteins (OncoE6™ Cervical Test) in remote areas in Brazil.
Women living in rural villages in proximity to Coari city, Amazonas, Brazil were invited to participate in a cervical cancer screening study. 412 subjects were enrolled; there were no refusals. In addition to E6 protein detection, DNA was extracted from the brushes and evaluated for HPV genotypes by PCR (PGMY09/11), followed by typing by the Papillocheck™ if positive. Subjects who were found to be positive for OncoE6 or HPV-DNA were referred for colposcopy.
For 110 subjects (27%) this was the first cervical cancer exam. Overall the HPV-DNA prevalence was 19.1% (n = 79); 1.4% (n = 6) were positive by the OncoE6 Test. Fifty-six women attended the invitation for colposcopy where nine had an abnormal cervix and were subsequently biopsied. Histopathological analysis revealed 2 CIN3, 2 carcinomas and 5 CIN1. OncoE6 called two out of the three HPV 16 or 18 associated CIN3+ lesions.
The findings suggest that self-administered sample collection in combination with OncoE6 Test is feasible in this population. This could enable expanded screening coverage while ensuring a high specificity which is imperative given the remote geographic location, since women bearing abnormal test results would necessitate travel and logistical burden to access colposcopy and treatment.</description><subject>Analysis</subject><subject>Biology and life sciences</subject><subject>Cancer</subject><subject>Cancer screening</subject><subject>Carcinoma</subject><subject>Cellular biology</subject><subject>Cervical cancer</subject><subject>Colposcopy</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>DNA</subject><subject>E6 protein</subject><subject>Genotypes</subject><subject>Human papillomavirus</subject><subject>Lesions</subject><subject>Malignancy</subject><subject>Medical screening</subject><subject>Medicine and Health Sciences</subject><subject>Papillomavirus infections</subject><subject>People and places</subject><subject>Proteins</subject><subject>Public 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coupled to the detection of HPV 16 and 18 E6 protein: A promising option for detection of cervical malignancies in remote areas</title><author>Torres, Kátia Luz ; Mariño, Josiane Montanho ; Pires Rocha, Danielle Albuquerque ; de Mello, Mônica Bandeira ; de Melo Farah, Heydy Halanna ; Reis, Renato Dos Santos ; Alves, Valquíria do Carmo Rodrigues ; Gomes, Edson ; Martins, Toni Ricardo ; Soares, Ana Carolina ; de Oliveira, Cristina Mendes ; Levi, José Eduardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c585t-a7fa40500aba09fb9986fd1a22fa6aad63b63e76c48eb700708b350f33d8bc093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Analysis</topic><topic>Biology and life sciences</topic><topic>Cancer</topic><topic>Cancer screening</topic><topic>Carcinoma</topic><topic>Cellular biology</topic><topic>Cervical cancer</topic><topic>Colposcopy</topic><topic>Deoxyribonucleic 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One</addtitle><date>2018-07-23</date><risdate>2018</risdate><volume>13</volume><issue>7</issue><spage>e0201262</spage><epage>e0201262</epage><pages>e0201262-e0201262</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>To evaluate both the performance and acceptability of a method coupling self-sampling with detection of cervical malignancy via elevated HPV 16 and 18 E6 oncoproteins (OncoE6™ Cervical Test) in remote areas in Brazil.
Women living in rural villages in proximity to Coari city, Amazonas, Brazil were invited to participate in a cervical cancer screening study. 412 subjects were enrolled; there were no refusals. In addition to E6 protein detection, DNA was extracted from the brushes and evaluated for HPV genotypes by PCR (PGMY09/11), followed by typing by the Papillocheck™ if positive. Subjects who were found to be positive for OncoE6 or HPV-DNA were referred for colposcopy.
For 110 subjects (27%) this was the first cervical cancer exam. Overall the HPV-DNA prevalence was 19.1% (n = 79); 1.4% (n = 6) were positive by the OncoE6 Test. Fifty-six women attended the invitation for colposcopy where nine had an abnormal cervix and were subsequently biopsied. Histopathological analysis revealed 2 CIN3, 2 carcinomas and 5 CIN1. OncoE6 called two out of the three HPV 16 or 18 associated CIN3+ lesions.
The findings suggest that self-administered sample collection in combination with OncoE6 Test is feasible in this population. This could enable expanded screening coverage while ensuring a high specificity which is imperative given the remote geographic location, since women bearing abnormal test results would necessitate travel and logistical burden to access colposcopy and treatment.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30036381</pmid><doi>10.1371/journal.pone.0201262</doi><orcidid>https://orcid.org/0000-0002-3557-2717</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-07, Vol.13 (7), p.e0201262-e0201262 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2074111378 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Analysis Biology and life sciences Cancer Cancer screening Carcinoma Cellular biology Cervical cancer Colposcopy Deoxyribonucleic acid Diagnosis DNA E6 protein Genotypes Human papillomavirus Lesions Malignancy Medical screening Medicine and Health Sciences Papillomavirus infections People and places Proteins Public health Remote regions Research and Analysis Methods Risk factors Rural areas Sampling Systematic review Womens health |
| title | Self-sampling coupled to the detection of HPV 16 and 18 E6 protein: A promising option for detection of cervical malignancies in remote areas |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T20%3A47%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Self-sampling%20coupled%20to%20the%20detection%20of%20HPV%2016%20and%2018%20E6%20protein:%20A%20promising%20option%20for%20detection%20of%20cervical%20malignancies%20in%20remote%20areas&rft.jtitle=PloS%20one&rft.au=Torres,%20K%C3%A1tia%20Luz&rft.date=2018-07-23&rft.volume=13&rft.issue=7&rft.spage=e0201262&rft.epage=e0201262&rft.pages=e0201262-e0201262&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0201262&rft_dat=%3Cgale_plos_%3EA547447024%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2074111378&rft_id=info:pmid/30036381&rft_galeid=A547447024&rft_doaj_id=oai_doaj_org_article_0c3bb69853654265821af81d3c349ea1&rfr_iscdi=true |