Maternal total cell-free DNA in preeclampsia and fetal growth restriction: Evidence of differences in maternal response to abnormal implantation
Preeclampsia and fetal growth restriction are obstetrical syndromes associated with abnormal placental implantation and changes in the activation status of maternal leukocytes. This study is aimed to determine by a simple, rapid fluorescent assay the changes in maternal serum total cell-free DNA (t-...
Gespeichert in:
| Veröffentlicht in: | PloS one 2018-07, Vol.13 (7), p.e0200360-e0200360 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0200360 |
|---|---|
| container_issue | 7 |
| container_start_page | e0200360 |
| container_title | PloS one |
| container_volume | 13 |
| creator | Rafaeli-Yehudai, Tal Imterat, Majdi Douvdevani, Amos Tirosh, Dan Benshalom-Tirosh, Neta Mastrolia, Salvatore Andrea Beer-Weisel, Ruthy Klaitman, Vered Riff, Reut Greenbaum, Shirley Alioshin, Alex Rodavsky Hanegbi, Gal Loverro, Giuseppe Catalano, Mariana Rita Erez, Offer |
| description | Preeclampsia and fetal growth restriction are obstetrical syndromes associated with abnormal placental implantation and changes in the activation status of maternal leukocytes. This study is aimed to determine by a simple, rapid fluorescent assay the changes in maternal serum total cell-free DNA (t-cfDNA) concentrations in women with preeclampsia and those with fetal growth restriction (FGR).
A cross-sectional study was conducted measuring maternal serum t-cfDNA concentrations. Women were classified into the following groups: 1) patients with preeclampsia (n = 21); 2) FGR-estimated fetal weight below the 10thpercentile (n = 28); and 3) normal pregnancy (n = 39). Serum samples were directly assayed for t-cfDNA using a rapid fluorescent SYBR Gold assay. Elevated maternal serum t-cfDNA concentrations were defined as a cutoff>850ng/ml. Nonparametric statistics were used for analysis.
Women with preeclampsia had a higher median maternal serum concentration (802 ng/ml, 400-2272 ng/ml) than women with a normal pregnancy (499 ng/ml, 0-1892 ng/ml, p = 0.004) and those with FGR (484 ng/ml, 72-2187 ng/ml, p = 0.012). Moreover, even patients with FGR |
| doi_str_mv | 10.1371/journal.pone.0200360 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2068892959</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A546354638</galeid><doaj_id>oai_doaj_org_article_ca65dff7b7ea43dda8475a6efae25e75</doaj_id><sourcerecordid>A546354638</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-a2322b88b7e65d4a2885832f9330a2b726c409cd5632363d8fd44718fd11a1713</originalsourceid><addsrcrecordid>eNqNk91u0zAUxyMEYqPwBggiISG4aHHsxEl2gVSNAZUGk_i6tU4du3WV2MF2BrwFj4xN06lBu0BRYsf-nf_5sE-SPM7QIiNl9mpnBquhXfRGiwXCCBGK7iSnWU3wnGJE7h7NT5IHzu0QKkhF6f3khCCEshyR0-T3B_Ai6qTe-PDlom3n0gqRvvm4TJVO-zDnLXS9U5CCblIpIrex5offplY4bxX3yuiz9OJaNUJzkRqZNkpKYeOfiyrdwUswCAE7EdylsNbGdmFRdX0L2kOUeZjck9A68WgcZ8nXtxdfzt_PL6_erc6Xl3NOa-zngAnG66pal4IWTQ64qoqKYFkTggCvS0x5jmreFJRgQklTySbPyywMWQZZmZFZ8nSv27fGsbGYjmFEq6rGdVEHYrUnGgM71lvVgf3FDCj2d8HYDQPrFW8F4xCCkLIM0UBOmgaqvCyACgkCF6Isgtbr0duw7kTDhfYW2onodEerLduYa0ZRjsuQxCx5MQpY830IVWedcvGwQAszxLhLFBLNKAros3_Q27MbqQ2EBJSWJvjlUZQti5yS-FaBWtxChacRneLh5kkV1icGLycGgfHip9_A4Bxbff70_-zVtyn7_IjdCmj91pl2iFfGTcF8D3JrnLNC3hQ5Qyw2zqEaLDYOGxsnmD05PqAbo0OnkD8ZoxS3</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2068892959</pqid></control><display><type>article</type><title>Maternal total cell-free DNA in preeclampsia and fetal growth restriction: Evidence of differences in maternal response to abnormal implantation</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Rafaeli-Yehudai, Tal ; Imterat, Majdi ; Douvdevani, Amos ; Tirosh, Dan ; Benshalom-Tirosh, Neta ; Mastrolia, Salvatore Andrea ; Beer-Weisel, Ruthy ; Klaitman, Vered ; Riff, Reut ; Greenbaum, Shirley ; Alioshin, Alex ; Rodavsky Hanegbi, Gal ; Loverro, Giuseppe ; Catalano, Mariana Rita ; Erez, Offer</creator><contributor>Sánchez-Margalet, Víctor</contributor><creatorcontrib>Rafaeli-Yehudai, Tal ; Imterat, Majdi ; Douvdevani, Amos ; Tirosh, Dan ; Benshalom-Tirosh, Neta ; Mastrolia, Salvatore Andrea ; Beer-Weisel, Ruthy ; Klaitman, Vered ; Riff, Reut ; Greenbaum, Shirley ; Alioshin, Alex ; Rodavsky Hanegbi, Gal ; Loverro, Giuseppe ; Catalano, Mariana Rita ; Erez, Offer ; Sánchez-Margalet, Víctor</creatorcontrib><description>Preeclampsia and fetal growth restriction are obstetrical syndromes associated with abnormal placental implantation and changes in the activation status of maternal leukocytes. This study is aimed to determine by a simple, rapid fluorescent assay the changes in maternal serum total cell-free DNA (t-cfDNA) concentrations in women with preeclampsia and those with fetal growth restriction (FGR).
A cross-sectional study was conducted measuring maternal serum t-cfDNA concentrations. Women were classified into the following groups: 1) patients with preeclampsia (n = 21); 2) FGR-estimated fetal weight below the 10thpercentile (n = 28); and 3) normal pregnancy (n = 39). Serum samples were directly assayed for t-cfDNA using a rapid fluorescent SYBR Gold assay. Elevated maternal serum t-cfDNA concentrations were defined as a cutoff>850ng/ml. Nonparametric statistics were used for analysis.
Women with preeclampsia had a higher median maternal serum concentration (802 ng/ml, 400-2272 ng/ml) than women with a normal pregnancy (499 ng/ml, 0-1892 ng/ml, p = 0.004) and those with FGR (484 ng/ml, 72-2187 ng/ml, p = 0.012). Moreover, even patients with FGR <5th percentile and abnormal Doppler had a lower median maternal serum t-cfDNA than those with preeclampsia (median 487 ng/ml, 144-1971 ng/ml, p = 0.022). The median concentration of t-cfDNA did not differ between women with a normal pregnancy and those with FGR (p = 0.54), as well as those with fetuses <5th percentile and abnormal Doppler (p = 0.7). Women with preeclampsia had a higher proportion of elevated t-cfDNA than those with a normal pregnancy (p = 0.015) and patients with FGR (p = 0.025).
Preeclampsia is associated with higher maternal serum t-cfDNA concentration than normal pregnancy or FGR. This observation may reflect an increased systemic activation of the maternal inflammation, rather than placental; this assumption is supported by the fact that we did not observe a significant change in the maternal serum t-cfDNA in patients with placental-mediated FGR.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0200360</identifier><identifier>PMID: 30001403</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Activation ; Apoptosis ; Assaying ; Biochemistry ; Biology and Life Sciences ; Biomarkers ; Cell activation ; Deoxyribonucleic acid ; DNA ; Fetal growth retardation ; Fetuses ; Fluorescence ; Genetic aspects ; Gold ; Gynecology ; Health aspects ; Health risk assessment ; Implantation ; Leukocytes ; Median (statistics) ; Medicine ; Medicine and Health Sciences ; Morphogenesis ; Neutrophils ; Nonparametric statistics ; Obstetrics ; Patients ; Placenta ; Plasma ; Pre-eclampsia ; Preeclampsia ; Pregnancy ; Pregnant women ; Prenatal development ; Risk factors ; Statistical analysis ; Statistical methods ; White blood cells</subject><ispartof>PloS one, 2018-07, Vol.13 (7), p.e0200360-e0200360</ispartof><rights>COPYRIGHT 2018 Public Library of Science</rights><rights>2018 Rafaeli-Yehudai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Rafaeli-Yehudai et al 2018 Rafaeli-Yehudai et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a2322b88b7e65d4a2885832f9330a2b726c409cd5632363d8fd44718fd11a1713</citedby><cites>FETCH-LOGICAL-c692t-a2322b88b7e65d4a2885832f9330a2b726c409cd5632363d8fd44718fd11a1713</cites><orcidid>0000-0002-0680-7652 ; 0000-0002-2213-8129</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042756/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042756/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30001403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sánchez-Margalet, Víctor</contributor><creatorcontrib>Rafaeli-Yehudai, Tal</creatorcontrib><creatorcontrib>Imterat, Majdi</creatorcontrib><creatorcontrib>Douvdevani, Amos</creatorcontrib><creatorcontrib>Tirosh, Dan</creatorcontrib><creatorcontrib>Benshalom-Tirosh, Neta</creatorcontrib><creatorcontrib>Mastrolia, Salvatore Andrea</creatorcontrib><creatorcontrib>Beer-Weisel, Ruthy</creatorcontrib><creatorcontrib>Klaitman, Vered</creatorcontrib><creatorcontrib>Riff, Reut</creatorcontrib><creatorcontrib>Greenbaum, Shirley</creatorcontrib><creatorcontrib>Alioshin, Alex</creatorcontrib><creatorcontrib>Rodavsky Hanegbi, Gal</creatorcontrib><creatorcontrib>Loverro, Giuseppe</creatorcontrib><creatorcontrib>Catalano, Mariana Rita</creatorcontrib><creatorcontrib>Erez, Offer</creatorcontrib><title>Maternal total cell-free DNA in preeclampsia and fetal growth restriction: Evidence of differences in maternal response to abnormal implantation</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Preeclampsia and fetal growth restriction are obstetrical syndromes associated with abnormal placental implantation and changes in the activation status of maternal leukocytes. This study is aimed to determine by a simple, rapid fluorescent assay the changes in maternal serum total cell-free DNA (t-cfDNA) concentrations in women with preeclampsia and those with fetal growth restriction (FGR).
A cross-sectional study was conducted measuring maternal serum t-cfDNA concentrations. Women were classified into the following groups: 1) patients with preeclampsia (n = 21); 2) FGR-estimated fetal weight below the 10thpercentile (n = 28); and 3) normal pregnancy (n = 39). Serum samples were directly assayed for t-cfDNA using a rapid fluorescent SYBR Gold assay. Elevated maternal serum t-cfDNA concentrations were defined as a cutoff>850ng/ml. Nonparametric statistics were used for analysis.
Women with preeclampsia had a higher median maternal serum concentration (802 ng/ml, 400-2272 ng/ml) than women with a normal pregnancy (499 ng/ml, 0-1892 ng/ml, p = 0.004) and those with FGR (484 ng/ml, 72-2187 ng/ml, p = 0.012). Moreover, even patients with FGR <5th percentile and abnormal Doppler had a lower median maternal serum t-cfDNA than those with preeclampsia (median 487 ng/ml, 144-1971 ng/ml, p = 0.022). The median concentration of t-cfDNA did not differ between women with a normal pregnancy and those with FGR (p = 0.54), as well as those with fetuses <5th percentile and abnormal Doppler (p = 0.7). Women with preeclampsia had a higher proportion of elevated t-cfDNA than those with a normal pregnancy (p = 0.015) and patients with FGR (p = 0.025).
Preeclampsia is associated with higher maternal serum t-cfDNA concentration than normal pregnancy or FGR. This observation may reflect an increased systemic activation of the maternal inflammation, rather than placental; this assumption is supported by the fact that we did not observe a significant change in the maternal serum t-cfDNA in patients with placental-mediated FGR.</description><subject>Activation</subject><subject>Apoptosis</subject><subject>Assaying</subject><subject>Biochemistry</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Cell activation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Fetal growth retardation</subject><subject>Fetuses</subject><subject>Fluorescence</subject><subject>Genetic aspects</subject><subject>Gold</subject><subject>Gynecology</subject><subject>Health aspects</subject><subject>Health risk assessment</subject><subject>Implantation</subject><subject>Leukocytes</subject><subject>Median (statistics)</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Morphogenesis</subject><subject>Neutrophils</subject><subject>Nonparametric statistics</subject><subject>Obstetrics</subject><subject>Patients</subject><subject>Placenta</subject><subject>Plasma</subject><subject>Pre-eclampsia</subject><subject>Preeclampsia</subject><subject>Pregnancy</subject><subject>Pregnant women</subject><subject>Prenatal development</subject><subject>Risk factors</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>White blood cells</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk91u0zAUxyMEYqPwBggiISG4aHHsxEl2gVSNAZUGk_i6tU4du3WV2MF2BrwFj4xN06lBu0BRYsf-nf_5sE-SPM7QIiNl9mpnBquhXfRGiwXCCBGK7iSnWU3wnGJE7h7NT5IHzu0QKkhF6f3khCCEshyR0-T3B_Ai6qTe-PDlom3n0gqRvvm4TJVO-zDnLXS9U5CCblIpIrex5offplY4bxX3yuiz9OJaNUJzkRqZNkpKYeOfiyrdwUswCAE7EdylsNbGdmFRdX0L2kOUeZjck9A68WgcZ8nXtxdfzt_PL6_erc6Xl3NOa-zngAnG66pal4IWTQ64qoqKYFkTggCvS0x5jmreFJRgQklTySbPyywMWQZZmZFZ8nSv27fGsbGYjmFEq6rGdVEHYrUnGgM71lvVgf3FDCj2d8HYDQPrFW8F4xCCkLIM0UBOmgaqvCyACgkCF6Isgtbr0duw7kTDhfYW2onodEerLduYa0ZRjsuQxCx5MQpY830IVWedcvGwQAszxLhLFBLNKAros3_Q27MbqQ2EBJSWJvjlUZQti5yS-FaBWtxChacRneLh5kkV1icGLycGgfHip9_A4Bxbff70_-zVtyn7_IjdCmj91pl2iFfGTcF8D3JrnLNC3hQ5Qyw2zqEaLDYOGxsnmD05PqAbo0OnkD8ZoxS3</recordid><startdate>20180712</startdate><enddate>20180712</enddate><creator>Rafaeli-Yehudai, Tal</creator><creator>Imterat, Majdi</creator><creator>Douvdevani, Amos</creator><creator>Tirosh, Dan</creator><creator>Benshalom-Tirosh, Neta</creator><creator>Mastrolia, Salvatore Andrea</creator><creator>Beer-Weisel, Ruthy</creator><creator>Klaitman, Vered</creator><creator>Riff, Reut</creator><creator>Greenbaum, Shirley</creator><creator>Alioshin, Alex</creator><creator>Rodavsky Hanegbi, Gal</creator><creator>Loverro, Giuseppe</creator><creator>Catalano, Mariana Rita</creator><creator>Erez, Offer</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-0680-7652</orcidid><orcidid>https://orcid.org/0000-0002-2213-8129</orcidid></search><sort><creationdate>20180712</creationdate><title>Maternal total cell-free DNA in preeclampsia and fetal growth restriction: Evidence of differences in maternal response to abnormal implantation</title><author>Rafaeli-Yehudai, Tal ; Imterat, Majdi ; Douvdevani, Amos ; Tirosh, Dan ; Benshalom-Tirosh, Neta ; Mastrolia, Salvatore Andrea ; Beer-Weisel, Ruthy ; Klaitman, Vered ; Riff, Reut ; Greenbaum, Shirley ; Alioshin, Alex ; Rodavsky Hanegbi, Gal ; Loverro, Giuseppe ; Catalano, Mariana Rita ; Erez, Offer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-a2322b88b7e65d4a2885832f9330a2b726c409cd5632363d8fd44718fd11a1713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Activation</topic><topic>Apoptosis</topic><topic>Assaying</topic><topic>Biochemistry</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Cell activation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Fetal growth retardation</topic><topic>Fetuses</topic><topic>Fluorescence</topic><topic>Genetic aspects</topic><topic>Gold</topic><topic>Gynecology</topic><topic>Health aspects</topic><topic>Health risk assessment</topic><topic>Implantation</topic><topic>Leukocytes</topic><topic>Median (statistics)</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Morphogenesis</topic><topic>Neutrophils</topic><topic>Nonparametric statistics</topic><topic>Obstetrics</topic><topic>Patients</topic><topic>Placenta</topic><topic>Plasma</topic><topic>Pre-eclampsia</topic><topic>Preeclampsia</topic><topic>Pregnancy</topic><topic>Pregnant women</topic><topic>Prenatal development</topic><topic>Risk factors</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><topic>White blood cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rafaeli-Yehudai, Tal</creatorcontrib><creatorcontrib>Imterat, Majdi</creatorcontrib><creatorcontrib>Douvdevani, Amos</creatorcontrib><creatorcontrib>Tirosh, Dan</creatorcontrib><creatorcontrib>Benshalom-Tirosh, Neta</creatorcontrib><creatorcontrib>Mastrolia, Salvatore Andrea</creatorcontrib><creatorcontrib>Beer-Weisel, Ruthy</creatorcontrib><creatorcontrib>Klaitman, Vered</creatorcontrib><creatorcontrib>Riff, Reut</creatorcontrib><creatorcontrib>Greenbaum, Shirley</creatorcontrib><creatorcontrib>Alioshin, Alex</creatorcontrib><creatorcontrib>Rodavsky Hanegbi, Gal</creatorcontrib><creatorcontrib>Loverro, Giuseppe</creatorcontrib><creatorcontrib>Catalano, Mariana Rita</creatorcontrib><creatorcontrib>Erez, Offer</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rafaeli-Yehudai, Tal</au><au>Imterat, Majdi</au><au>Douvdevani, Amos</au><au>Tirosh, Dan</au><au>Benshalom-Tirosh, Neta</au><au>Mastrolia, Salvatore Andrea</au><au>Beer-Weisel, Ruthy</au><au>Klaitman, Vered</au><au>Riff, Reut</au><au>Greenbaum, Shirley</au><au>Alioshin, Alex</au><au>Rodavsky Hanegbi, Gal</au><au>Loverro, Giuseppe</au><au>Catalano, Mariana Rita</au><au>Erez, Offer</au><au>Sánchez-Margalet, Víctor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maternal total cell-free DNA in preeclampsia and fetal growth restriction: Evidence of differences in maternal response to abnormal implantation</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-07-12</date><risdate>2018</risdate><volume>13</volume><issue>7</issue><spage>e0200360</spage><epage>e0200360</epage><pages>e0200360-e0200360</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Preeclampsia and fetal growth restriction are obstetrical syndromes associated with abnormal placental implantation and changes in the activation status of maternal leukocytes. This study is aimed to determine by a simple, rapid fluorescent assay the changes in maternal serum total cell-free DNA (t-cfDNA) concentrations in women with preeclampsia and those with fetal growth restriction (FGR).
A cross-sectional study was conducted measuring maternal serum t-cfDNA concentrations. Women were classified into the following groups: 1) patients with preeclampsia (n = 21); 2) FGR-estimated fetal weight below the 10thpercentile (n = 28); and 3) normal pregnancy (n = 39). Serum samples were directly assayed for t-cfDNA using a rapid fluorescent SYBR Gold assay. Elevated maternal serum t-cfDNA concentrations were defined as a cutoff>850ng/ml. Nonparametric statistics were used for analysis.
Women with preeclampsia had a higher median maternal serum concentration (802 ng/ml, 400-2272 ng/ml) than women with a normal pregnancy (499 ng/ml, 0-1892 ng/ml, p = 0.004) and those with FGR (484 ng/ml, 72-2187 ng/ml, p = 0.012). Moreover, even patients with FGR <5th percentile and abnormal Doppler had a lower median maternal serum t-cfDNA than those with preeclampsia (median 487 ng/ml, 144-1971 ng/ml, p = 0.022). The median concentration of t-cfDNA did not differ between women with a normal pregnancy and those with FGR (p = 0.54), as well as those with fetuses <5th percentile and abnormal Doppler (p = 0.7). Women with preeclampsia had a higher proportion of elevated t-cfDNA than those with a normal pregnancy (p = 0.015) and patients with FGR (p = 0.025).
Preeclampsia is associated with higher maternal serum t-cfDNA concentration than normal pregnancy or FGR. This observation may reflect an increased systemic activation of the maternal inflammation, rather than placental; this assumption is supported by the fact that we did not observe a significant change in the maternal serum t-cfDNA in patients with placental-mediated FGR.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30001403</pmid><doi>10.1371/journal.pone.0200360</doi><orcidid>https://orcid.org/0000-0002-0680-7652</orcidid><orcidid>https://orcid.org/0000-0002-2213-8129</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2018-07, Vol.13 (7), p.e0200360-e0200360 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2068892959 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Activation Apoptosis Assaying Biochemistry Biology and Life Sciences Biomarkers Cell activation Deoxyribonucleic acid DNA Fetal growth retardation Fetuses Fluorescence Genetic aspects Gold Gynecology Health aspects Health risk assessment Implantation Leukocytes Median (statistics) Medicine Medicine and Health Sciences Morphogenesis Neutrophils Nonparametric statistics Obstetrics Patients Placenta Plasma Pre-eclampsia Preeclampsia Pregnancy Pregnant women Prenatal development Risk factors Statistical analysis Statistical methods White blood cells |
| title | Maternal total cell-free DNA in preeclampsia and fetal growth restriction: Evidence of differences in maternal response to abnormal implantation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T13%3A30%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Maternal%20total%20cell-free%20DNA%20in%20preeclampsia%20and%20fetal%20growth%20restriction:%20Evidence%20of%20differences%20in%20maternal%20response%20to%20abnormal%20implantation&rft.jtitle=PloS%20one&rft.au=Rafaeli-Yehudai,%20Tal&rft.date=2018-07-12&rft.volume=13&rft.issue=7&rft.spage=e0200360&rft.epage=e0200360&rft.pages=e0200360-e0200360&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0200360&rft_dat=%3Cgale_plos_%3EA546354638%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2068892959&rft_id=info:pmid/30001403&rft_galeid=A546354638&rft_doaj_id=oai_doaj_org_article_ca65dff7b7ea43dda8475a6efae25e75&rfr_iscdi=true |